Reviewed, UniProtKB/Swiss-Prot Q9P0R6 (GSKIP_HUMAN)

Names and origin

Protein names

Recommended name:
    GSK3-beta interaction protein
      Short name=GSKIP

Gene names

Name:	GSKIP
Synonyms:	C14orf129
ORF Names:	HSPC210

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with GSK3B. Ref.6
Subcellular location	Cytoplasm Ref.6.
Tissue specificity	Detected in heart, brain, placenta, liver, skeletal muscle, kidney, testis, lung and pancreas. Ref.6
Sequence similarities	Belongs to the UPF0279 family.
Sequence caution	The sequence AAF36130.1 differs from that shown. Reason: Frameshift at position 132.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GSK3B	P49841	1	EBI-1052580,EBI-373586

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

GSK3-beta interaction protein

PRO_0000220951

Regions

Region

115 – 139

25

Interaction with GSK3B

Experimental info

Mutagenesis

130

1

L → P: Loss of interaction with GSK3B. Ref.6

Sequence conflict

46

1

F → S in BAA91380. Ref.2

Secondary structure

1

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139

Helix Strand Turn

Details...

Beta strand

10 – 13

4

Helix

33 – 44

12

Helix

45 – 47

3

Beta strand

48 – 53

6

Beta strand

63 – 69

7

Beta strand

75 – 81

7

Beta strand

84 – 91

8

Beta strand

103 – 107

5

Helix

108 – 114

7

Helix

116 – 135

20

Sequences

	Sequence		Length	Mass (Da)
	Q9P0R6-1 [UniParc]. Last modified September 9, 2003. Version 2. Checksum: A2D8BAF4B458B316	FASTA	139	15,648
	10 20 30 40 50 60 METDCNPMEL SSMSGFEEGS ELNGFEGTDM KDMRLEAEAV VNDVLFAVNN MFVSKSLRCA 70 80 90 100 110 120 DDVAYINVET KERNRYCLEL TEAGLKVVGY AFDQVDDHLQ TPYHETVYSL LDTLSPAYRE 130 AFGNALLQRL EALKRDGQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala and Colon.
[3]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	"GSKIP is homologous to the axin GSK3beta interaction domain and functions as a negative regulator of GSK3beta." Chou H.-Y., Howng S.-L., Cheng T.-S., Hsiao Y.-L., Lieu A.-S., Loh J.-K., Hwang S.-L., Lin C.-C., Hsu C.-M., Wang C., Lee C.-I., Lu P.-J., Chou C.-K., Huang C.-Y., Hong Y.-R. Biochemistry 45:11379-11389(2006) [PubMed: 16981698] [Abstract] Cited for: INTERACTION WITH GSK3B, SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-130, TISSUE SPECIFICITY.
[7]	"NMR structure of the human C14orf129 gene product, HSPC210. Northeast structural genomics target HR969." Northeast structural genomics consortium (NESG) Submitted (JAN-2005) to the PDB data bank Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF151044 mRNA. Translation: AAF36130.1. Frameshift.
AK000796 mRNA. Translation: BAA91380.1.
AK094654 mRNA. Translation: BAG52902.1.
AL359240 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81634.1.
BC004818 mRNA. Translation: AAH04818.1.

IPI

IPI00009374.

RefSeq

NP_057556.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SGO	NMR	-	A	1-139	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9P0R6. 3 interactions.

STRING

Q9P0R6.

Proteomic databases

PRIDE

Q9P0R6.

Genome annotation databases

Ensembl

ENST00000329856; ENSP00000330180; ENSG00000100744; Homo sapiens. [Genome view]
ENST00000438650; ENSP00000412315; ENSG00000100744; Homo sapiens. [Genome view]

GeneID

51527.

KEGG

hsa:51527.

UCSC

uc001yfj.2. human.

Organism-specific databases

CTD

51527.

HGNC

HGNC:20343. C14orf129.

PharmGKB

PA134987554.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG07796.

HOGENOM

HBG282296.

HOVERGEN

Q9P0R6.

InParanoid

Q9P0R6.

OMA

VETRERN.

OrthoDB

EOG9Z0DSB.

Gene expression databases

ArrayExpress

Q9P0R6.

Bgee

Q9P0R6.

CleanEx

HS_C14orf129.

Genevestigator

Q9P0R6.

Family and domain databases

InterPro

IPR007967. DUF727.
[Graphical view]

PANTHER

PTHR12490. DUF727. 1 hit.

Pfam

PF05303. DUF727. 1 hit.
[Graphical view]

ProDom

PD080843. DUF727. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other Resources

NextBio

55240.

Entry information

Entry name

GSKIP_HUMAN

Accession

Primary (citable) accession number: Q9P0R6
Secondary accession number(s): B3KSZ0, Q9BST1, Q9NWK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 9, 2003
Last sequence update:	September 9, 2003
Last modified:	January 19, 2010
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.