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Q9P0N8

- MARH2_HUMAN

UniProt

Q9P0N8 - MARH2_HUMAN

Protein

E3 ubiquitin-protein ligase MARCH2

Gene

MARCH2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6.3 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri56 – 11661RING-CH-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase MARCH2 (EC:6.3.2.-)
    Alternative name(s):
    Membrane-associated RING finger protein 2
    Membrane-associated RING-CH protein II
    Short name:
    MARCH-II
    RING finger protein 172
    Gene namesi
    Name:MARCH2
    Synonyms:RNF172
    ORF Names:HSPC240
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:28038. MARCH2.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication. Lysosome membrane 1 Publication; Multi-pass membrane protein 1 Publication. Endosome membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: UniProtKB
    2. endoplasmic reticulum Source: UniProtKB
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. endosome membrane Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW
    6. lysosomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Endosome, Lysosome, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134993565.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246E3 ubiquitin-protein ligase MARCH2PRO_0000055925Add
    BLAST

    Proteomic databases

    PaxDbiQ9P0N8.
    PRIDEiQ9P0N8.

    Expressioni

    Tissue specificityi

    Broadly expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ9P0N8.
    BgeeiQ9P0N8.
    CleanExiHS_MARCH2.
    GenevestigatoriQ9P0N8.

    Organism-specific databases

    HPAiHPA014063.

    Interactioni

    Subunit structurei

    Interacts with STX6 By similarity. Interacts with MARCH3.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi119413. 9 interactions.
    STRINGi9606.ENSP00000215555.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P0N8.
    SMRiQ9P0N8. Positions 57-118.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei138 – 15821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    The RING-CH-type zinc finger domain is required for E3 ligase activity.

    Sequence similaritiesi

    Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri56 – 11661RING-CH-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5183.
    HOGENOMiHOG000293394.
    HOVERGENiHBG052411.
    InParanoidiQ9P0N8.
    KOiK10657.
    OMAiICHEGAG.
    OrthoDBiEOG7B05F1.
    PhylomeDBiQ9P0N8.
    TreeFamiTF319557.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR011016. Znf_RING-CH.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF12906. RINGv. 1 hit.
    [Graphical view]
    SMARTiSM00744. RINGv. 1 hit.
    [Graphical view]
    PROSITEiPS51292. ZF_RING_CH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9P0N8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTTGDCCHLP GSLCDCSGSP AFSKVVEATG LGPPQYVAQV TSRDGRLLST    50
    VIRALDTPSD GPFCRICHEG ANGECLLSPC GCTGTLGAVH KSCLEKWLSS 100
    SNTSYCELCH TEFAVEKRPR PLTEWLKDPG PRTEKRTLCC DMVCFLFITP 150
    LAAISGWLCL RGAQDHLRLH SQLEAVGLIA LTIALFTIYV LWTLVSFRYH 200
    CQLYSEWRKT NQKVRLKIRE ADSPEGPQHS PLAAGLLKKV AEETPV 246
    Length:246
    Mass (Da):26,995
    Last modified:October 1, 2000 - v1
    Checksum:iFBD877D55211C929
    GO
    Isoform 2 (identifier: Q9P0N8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         125-194: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:176
    Mass (Da):19,099
    Checksum:i1AA18937FB6F7652
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti54 – 541A → T.1 Publication
    Corresponds to variant rs1133893 [ dbSNP | Ensembl ].
    VAR_030303
    Natural varianti219 – 2191R → P.
    Corresponds to variant rs34099346 [ dbSNP | Ensembl ].
    VAR_053638

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei125 – 19470Missing in isoform 2. CuratedVSP_041478Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB197929 mRNA. Translation: BAD89359.1.
    AF151074 mRNA. Translation: AAF36160.1.
    AC136469 Genomic DNA. No translation available.
    BC032624 mRNA. Translation: AAH32624.1.
    BC015910 mRNA. Translation: AAH15910.1.
    BC111388 mRNA. Translation: AAI11389.1.
    CCDSiCCDS12202.1. [Q9P0N8-1]
    CCDS32894.1. [Q9P0N8-2]
    RefSeqiNP_001005415.1. NM_001005415.1. [Q9P0N8-1]
    NP_001005416.1. NM_001005416.1. [Q9P0N8-2]
    NP_057580.3. NM_016496.4. [Q9P0N8-1]
    XP_006722825.1. XM_006722762.1. [Q9P0N8-1]
    XP_006722826.1. XM_006722763.1. [Q9P0N8-2]
    UniGeneiHs.631861.

    Genome annotation databases

    EnsembliENST00000215555; ENSP00000215555; ENSG00000099785. [Q9P0N8-1]
    ENST00000381035; ENSP00000370423; ENSG00000099785. [Q9P0N8-2]
    ENST00000602117; ENSP00000471536; ENSG00000099785. [Q9P0N8-1]
    GeneIDi51257.
    KEGGihsa:51257.
    UCSCiuc002mjv.3. human. [Q9P0N8-1]
    uc002mjx.3. human. [Q9P0N8-2]

    Polymorphism databases

    DMDMi57012977.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB197929 mRNA. Translation: BAD89359.1 .
    AF151074 mRNA. Translation: AAF36160.1 .
    AC136469 Genomic DNA. No translation available.
    BC032624 mRNA. Translation: AAH32624.1 .
    BC015910 mRNA. Translation: AAH15910.1 .
    BC111388 mRNA. Translation: AAI11389.1 .
    CCDSi CCDS12202.1. [Q9P0N8-1 ]
    CCDS32894.1. [Q9P0N8-2 ]
    RefSeqi NP_001005415.1. NM_001005415.1. [Q9P0N8-1 ]
    NP_001005416.1. NM_001005416.1. [Q9P0N8-2 ]
    NP_057580.3. NM_016496.4. [Q9P0N8-1 ]
    XP_006722825.1. XM_006722762.1. [Q9P0N8-1 ]
    XP_006722826.1. XM_006722763.1. [Q9P0N8-2 ]
    UniGenei Hs.631861.

    3D structure databases

    ProteinModelPortali Q9P0N8.
    SMRi Q9P0N8. Positions 57-118.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119413. 9 interactions.
    STRINGi 9606.ENSP00000215555.

    Polymorphism databases

    DMDMi 57012977.

    Proteomic databases

    PaxDbi Q9P0N8.
    PRIDEi Q9P0N8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000215555 ; ENSP00000215555 ; ENSG00000099785 . [Q9P0N8-1 ]
    ENST00000381035 ; ENSP00000370423 ; ENSG00000099785 . [Q9P0N8-2 ]
    ENST00000602117 ; ENSP00000471536 ; ENSG00000099785 . [Q9P0N8-1 ]
    GeneIDi 51257.
    KEGGi hsa:51257.
    UCSCi uc002mjv.3. human. [Q9P0N8-1 ]
    uc002mjx.3. human. [Q9P0N8-2 ]

    Organism-specific databases

    CTDi 51257.
    GeneCardsi GC19P008478.
    H-InvDB HIX0022474.
    HGNCi HGNC:28038. MARCH2.
    HPAi HPA014063.
    MIMi 613332. gene.
    neXtProti NX_Q9P0N8.
    PharmGKBi PA134993565.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5183.
    HOGENOMi HOG000293394.
    HOVERGENi HBG052411.
    InParanoidi Q9P0N8.
    KOi K10657.
    OMAi ICHEGAG.
    OrthoDBi EOG7B05F1.
    PhylomeDBi Q9P0N8.
    TreeFami TF319557.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    GeneWikii MARCH2.
    GenomeRNAii 51257.
    NextBioi 54414.
    PROi Q9P0N8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9P0N8.
    Bgeei Q9P0N8.
    CleanExi HS_MARCH2.
    Genevestigatori Q9P0N8.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR011016. Znf_RING-CH.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF12906. RINGv. 1 hit.
    [Graphical view ]
    SMARTi SM00744. RINGv. 1 hit.
    [Graphical view ]
    PROSITEi PS51292. ZF_RING_CH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking."
      Nakamura N., Fukuda H., Kato A., Hirose S.
      Mol. Biol. Cell 16:1696-1710(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
    2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Umbilical cord blood.
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-54.
      Tissue: Brain and Skin.
    5. "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins."
      Bartee E., Mansouri M., Hovey Nerenberg B.T., Gouveia K., Frueh K.
      J. Virol. 78:1109-1120(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    6. "MARCH-III is a novel component of endosomes with properties similar to those of MARCH-II."
      Fukuda H., Nakamura N., Hirose S.
      J. Biochem. 139:137-145(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MARCH3.

    Entry informationi

    Entry nameiMARH2_HUMAN
    AccessioniPrimary (citable) accession number: Q9P0N8
    Secondary accession number(s): A6NP10
    , Q5H785, Q8N5A3, Q96B78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 4, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3