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Q9P0N8 (MARH2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase MARCH2

EC=6.3.2.-
Alternative name(s):
Membrane-associated RING finger protein 2
Membrane-associated RING-CH protein II
Short name=MARCH-II
RING finger protein 172
Gene names
Name:MARCH2
Synonyms:RNF172
ORF Names:HSPC240
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May be involved in endosomal trafficking through interaction with STX6. Ref.1 Ref.5 Ref.6

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with STX6 By similarity. Interacts with MARCH3. Ref.6

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein By similarity Ref.5.

Tissue specificity

Broadly expressed. Ref.5

Domain

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Sequence similarities

Contains 1 RING-CH-type zinc finger.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P0N8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P0N8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     125-194: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246E3 ubiquitin-protein ligase MARCH2
PRO_0000055925

Regions

Transmembrane138 – 15821Helical; Potential
Transmembrane175 – 19521Helical; Potential
Zinc finger56 – 11661RING-CH-type

Natural variations

Alternative sequence125 – 19470Missing in isoform 2.
VSP_041478
Natural variant541A → T. Ref.4
Corresponds to variant rs1133893 [ dbSNP | Ensembl ].
VAR_030303
Natural variant2191R → P.
Corresponds to variant rs34099346 [ dbSNP | Ensembl ].
VAR_053638

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: FBD877D55211C929

FASTA24626,995
        10         20         30         40         50         60 
MTTGDCCHLP GSLCDCSGSP AFSKVVEATG LGPPQYVAQV TSRDGRLLST VIRALDTPSD 

        70         80         90        100        110        120 
GPFCRICHEG ANGECLLSPC GCTGTLGAVH KSCLEKWLSS SNTSYCELCH TEFAVEKRPR 

       130        140        150        160        170        180 
PLTEWLKDPG PRTEKRTLCC DMVCFLFITP LAAISGWLCL RGAQDHLRLH SQLEAVGLIA 

       190        200        210        220        230        240 
LTIALFTIYV LWTLVSFRYH CQLYSEWRKT NQKVRLKIRE ADSPEGPQHS PLAAGLLKKV 


AEETPV 

« Hide

Isoform 2 [UniParc].

Checksum: 1AA18937FB6F7652
Show »

FASTA17619,099

References

« Hide 'large scale' references
[1]"MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking."
Nakamura N., Fukuda H., Kato A., Hirose S.
Mol. Biol. Cell 16:1696-1710(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Umbilical cord blood.
[3]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT THR-54.
Tissue: Brain and Skin.
[5]"Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins."
Bartee E., Mansouri M., Hovey Nerenberg B.T., Gouveia K., Frueh K.
J. Virol. 78:1109-1120(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[6]"MARCH-III is a novel component of endosomes with properties similar to those of MARCH-II."
Fukuda H., Nakamura N., Hirose S.
J. Biochem. 139:137-145(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MARCH3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB197929 mRNA. Translation: BAD89359.1.
AF151074 mRNA. Translation: AAF36160.1.
AC136469 Genomic DNA. No translation available.
BC032624 mRNA. Translation: AAH32624.1.
BC015910 mRNA. Translation: AAH15910.1.
BC111388 mRNA. Translation: AAI11389.1.
CCDSCCDS12202.1. [Q9P0N8-1]
CCDS32894.1. [Q9P0N8-2]
RefSeqNP_001005415.1. NM_001005415.1. [Q9P0N8-1]
NP_001005416.1. NM_001005416.1. [Q9P0N8-2]
NP_057580.3. NM_016496.4. [Q9P0N8-1]
XP_006722825.1. XM_006722762.1. [Q9P0N8-1]
XP_006722826.1. XM_006722763.1. [Q9P0N8-2]
UniGeneHs.631861.

3D structure databases

ProteinModelPortalQ9P0N8.
SMRQ9P0N8. Positions 57-118.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119413. 9 interactions.
STRING9606.ENSP00000215555.

Polymorphism databases

DMDM57012977.

Proteomic databases

PaxDbQ9P0N8.
PRIDEQ9P0N8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000215555; ENSP00000215555; ENSG00000099785. [Q9P0N8-1]
ENST00000381035; ENSP00000370423; ENSG00000099785. [Q9P0N8-2]
ENST00000393944; ENSP00000377518; ENSG00000099785. [Q9P0N8-1]
ENST00000602117; ENSP00000471536; ENSG00000099785. [Q9P0N8-1]
GeneID51257.
KEGGhsa:51257.
UCSCuc002mjv.3. human. [Q9P0N8-1]
uc002mjx.3. human. [Q9P0N8-2]

Organism-specific databases

CTD51257.
GeneCardsGC19P008478.
H-InvDBHIX0022474.
HGNCHGNC:28038. MARCH2.
HPAHPA014063.
MIM613332. gene.
neXtProtNX_Q9P0N8.
PharmGKBPA134993565.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5183.
HOGENOMHOG000293394.
HOVERGENHBG052411.
InParanoidQ9P0N8.
KOK10657.
OMAICHEGAG.
OrthoDBEOG7B05F1.
PhylomeDBQ9P0N8.
TreeFamTF319557.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ9P0N8.
BgeeQ9P0N8.
CleanExHS_MARCH2.
GenevestigatorQ9P0N8.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF12906. RINGv. 1 hit.
[Graphical view]
SMARTSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEPS51292. ZF_RING_CH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMARCH2.
GenomeRNAi51257.
NextBio54414.
PROQ9P0N8.
SOURCESearch...

Entry information

Entry nameMARH2_HUMAN
AccessionPrimary (citable) accession number: Q9P0N8
Secondary accession number(s): A6NP10 expand/collapse secondary AC list , Q5H785, Q8N5A3, Q96B78
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM