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Q9P0M6

- H2AW_HUMAN

UniProt

Q9P0M6 - H2AW_HUMAN

Protein

Core histone macro-H2A.2

Gene

H2AFY2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.1 Publication

    GO - Molecular functioni

    1. chromatin DNA binding Source: UniProt
    2. transcription regulatory region DNA binding Source: UniProt

    GO - Biological processi

    1. brain development Source: UniProt
    2. chromatin modification Source: UniProtKB-KW
    3. dosage compensation Source: MGI
    4. establishment of protein localization to chromatin Source: UniProt
    5. negative regulation of gene expression, epigenetic Source: UniProt
    6. negative regulation of transcription from RNA polymerase II promoter Source: UniProt
    7. negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter Source: UniProt
    8. nucleosome assembly Source: InterPro

    Keywords - Molecular functioni

    Chromatin regulator

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Core histone macro-H2A.2
    Short name:
    Histone macroH2A2
    Short name:
    mH2A2
    Gene namesi
    Name:H2AFY2
    Synonyms:MACROH2A2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:14453. H2AFY2.

    Subcellular locationi

    Nucleus. Chromosome
    Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.

    GO - Cellular componenti

    1. Barr body Source: MGI
    2. extracellular vesicular exosome Source: UniProt
    3. nuclear chromatin Source: UniProt
    4. nucleosome Source: UniProtKB-KW
    5. nucleus Source: HPA

    Keywords - Cellular componenti

    Chromosome, Nucleosome core, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29118.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Core histone macro-H2A.2PRO_0000055320Add
    BLAST

    Proteomic databases

    MaxQBiQ9P0M6.
    PaxDbiQ9P0M6.
    PRIDEiQ9P0M6.

    PTM databases

    PhosphoSiteiQ9P0M6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9P0M6.
    BgeeiQ9P0M6.
    CleanExiHS_H2AFY2.
    GenevestigatoriQ9P0M6.

    Organism-specific databases

    HPAiHPA035865.

    Interactioni

    Subunit structurei

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

    Protein-protein interaction databases

    BioGridi120686. 9 interactions.
    DIPiDIP-48563N.
    IntActiQ9P0M6. 2 interactions.
    MINTiMINT-6609507.
    STRINGi9606.ENSP00000362352.

    Structurei

    Secondary structure

    1
    372
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi184 – 1907
    Beta strandi196 – 2016
    Helixi204 – 2096
    Beta strandi213 – 2197
    Helixi228 – 25124
    Beta strandi260 – 2645
    Beta strandi268 – 27710
    Helixi286 – 30318
    Beta strandi307 – 3115
    Helixi323 – 34018
    Beta strandi348 – 3536
    Helixi356 – 36712

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2XD7X-ray2.09A/B/C/D177-369[»]
    ProteinModelPortaliQ9P0M6.
    SMRiQ9P0M6. Positions 12-117, 177-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9P0M6.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 117116Histone H2AAdd
    BLAST
    Domaini184 – 370187MacroPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi118 – 16144Lys-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 histone H2A domain.Curated
    Contains 1 Macro domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2110.
    HOGENOMiHOG000234653.
    HOVERGENiHBG009342.
    InParanoidiQ9P0M6.
    KOiK11251.
    OMAiKVEGIVH.
    OrthoDBiEOG71G9VP.
    PhylomeDBiQ9P0M6.
    TreeFamiTF332276.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR021171. Core_histone_macro-H2A.
    IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR002119. Histone_H2A.
    IPR002589. Macro_dom.
    [Graphical view]
    PfamiPF00125. Histone. 1 hit.
    PF01661. Macro. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037942. Core_histone_macro-H2A. 1 hit.
    PRINTSiPR00620. HISTONEH2A.
    SMARTiSM00414. H2A. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.
    PROSITEiPS51154. MACRO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9P0M6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGRSGKKKM SKLSRSARAG VIFPVGRLMR YLKKGTFKYR ISVGAPVYMA    50
    AVIEYLAAEI LELAGNAARD NKKARIAPRH ILLAVANDEE LNQLLKGVTI 100
    ASGGVLPRIH PELLAKKRGT KGKSETILSP PPEKRGRKAT SGKKGGKKSK 150
    AAKPRTSKKS KPKDSDKEGT SNSTSEDGPG DGFTILSSKS LVLGQKLSLT 200
    QSDISHIGSM RVEGIVHPTT AEIDLKEDIG KALEKAGGKE FLETVKELRK 250
    SQGPLEVAEA AVSQSSGLAA KFVIHCHIPQ WGSDKCEEQL EETIKNCLSA 300
    AEDKKLKSVA FPPFPSGRNC FPKQTAAQVT LKAISAHFDD SSASSLKNVY 350
    FLLFDSESIG IYVQEMAKLD AK 372
    Length:372
    Mass (Da):40,058
    Last modified:January 23, 2007 - v3
    Checksum:iC4B35CD156031AF1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF336304 mRNA. Translation: AAK52471.1.
    AF151534 mRNA. Translation: AAF72101.1.
    AK022776 mRNA. Translation: BAB14239.1.
    AL731540 Genomic DNA. Translation: CAI13683.1.
    BC016172 mRNA. Translation: AAH16172.1.
    CCDSiCCDS7296.1.
    RefSeqiNP_061119.1. NM_018649.2.
    UniGeneiHs.499953.

    Genome annotation databases

    EnsembliENST00000373255; ENSP00000362352; ENSG00000099284.
    GeneIDi55506.
    KEGGihsa:55506.
    UCSCiuc001jqm.3. human.

    Polymorphism databases

    DMDMi12585260.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF336304 mRNA. Translation: AAK52471.1 .
    AF151534 mRNA. Translation: AAF72101.1 .
    AK022776 mRNA. Translation: BAB14239.1 .
    AL731540 Genomic DNA. Translation: CAI13683.1 .
    BC016172 mRNA. Translation: AAH16172.1 .
    CCDSi CCDS7296.1.
    RefSeqi NP_061119.1. NM_018649.2.
    UniGenei Hs.499953.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2XD7 X-ray 2.09 A/B/C/D 177-369 [» ]
    ProteinModelPortali Q9P0M6.
    SMRi Q9P0M6. Positions 12-117, 177-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120686. 9 interactions.
    DIPi DIP-48563N.
    IntActi Q9P0M6. 2 interactions.
    MINTi MINT-6609507.
    STRINGi 9606.ENSP00000362352.

    PTM databases

    PhosphoSitei Q9P0M6.

    Polymorphism databases

    DMDMi 12585260.

    Proteomic databases

    MaxQBi Q9P0M6.
    PaxDbi Q9P0M6.
    PRIDEi Q9P0M6.

    Protocols and materials databases

    DNASUi 55506.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000373255 ; ENSP00000362352 ; ENSG00000099284 .
    GeneIDi 55506.
    KEGGi hsa:55506.
    UCSCi uc001jqm.3. human.

    Organism-specific databases

    CTDi 55506.
    GeneCardsi GC10P071812.
    H-InvDB HIX0008890.
    HGNCi HGNC:14453. H2AFY2.
    HPAi HPA035865.
    neXtProti NX_Q9P0M6.
    PharmGKBi PA29118.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2110.
    HOGENOMi HOG000234653.
    HOVERGENi HBG009342.
    InParanoidi Q9P0M6.
    KOi K11251.
    OMAi KVEGIVH.
    OrthoDBi EOG71G9VP.
    PhylomeDBi Q9P0M6.
    TreeFami TF332276.

    Miscellaneous databases

    ChiTaRSi H2AFY2. human.
    EvolutionaryTracei Q9P0M6.
    GeneWikii H2AFY2.
    GenomeRNAii 55506.
    NextBioi 59903.
    PROi Q9P0M6.

    Gene expression databases

    ArrayExpressi Q9P0M6.
    Bgeei Q9P0M6.
    CleanExi HS_H2AFY2.
    Genevestigatori Q9P0M6.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR021171. Core_histone_macro-H2A.
    IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR002119. Histone_H2A.
    IPR002589. Macro_dom.
    [Graphical view ]
    Pfami PF00125. Histone. 1 hit.
    PF01661. Macro. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037942. Core_histone_macro-H2A. 1 hit.
    PRINTSi PR00620. HISTONEH2A.
    SMARTi SM00414. H2A. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    PROSITEi PS51154. MACRO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant."
      Chadwick B.P., Willard H.F.
      Hum. Mol. Genet. 10:1101-1113(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    2. "MACROH2A2, a new member of the MACROH2A core histone family."
      Costanzi C., Pehrson J.R.
      J. Biol. Chem. 276:21776-21784(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Uterus.
    6. "Formation of MacroH2A-containing senescence-associated heterochromatin foci and senescence driven by ASF1a and HIRA."
      Zhang R., Poustovoitov M.V., Ye X., Santos H.A., Chen W., Daganzo S.M., Erzberger J.P., Serebriiskii I.G., Canutescu A.A., Dunbrack R.L., Pehrson J.R., Berger J.M., Kaufman P.D., Adams P.D.
      Dev. Cell 8:19-30(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiH2AW_HUMAN
    AccessioniPrimary (citable) accession number: Q9P0M6
    Secondary accession number(s): Q5SQT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3