Reviewed, UniProtKB/Swiss-Prot Q9P0K8 (FOXJ2_HUMAN)

Names and origin

Protein names

Recommended name:
    Forkhead box protein J2
Alternative name(s):
    Fork head homologous X

Gene names

Name:	FOXJ2
Synonyms:	FHX

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.
Subcellular location	Nucleus.
Tissue specificity	Widely expressed.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.5 Ref.6
Sequence similarities	Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: HPA
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform FOXJ2.L (identifier: Q9P0K8-1)

Also known as: FHX.L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform FOXJ2.S (identifier: Q9P0K8-2)

Also known as: FHX.S;

The sequence of this isoform differs from the canonical sequence as follows:
     513-574: VNSYGHPQAP...PDDFDWDLIT → GTAPSQLPWRWRLC

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Forkhead box protein J2		PRO_0000091853
Regions
	DNA binding	66 – 143	78	Fork-head
	Compositional bias	266 – 270	5	Poly-Ser
	Compositional bias	291 – 294	4	Poly-Gln
	Compositional bias	295 – 298	4	Poly-Pro
	Compositional bias	299 – 306	8	Poly-Gln
	Compositional bias	313 – 321	9	Poly-Gln
	Compositional bias	390 – 395	6	Poly-Pro
Amino acid modifications
	Modified residue	161	1	Phosphoserine Ref.5
	Modified residue	259	1	Phosphotyrosine Ref.6
	Modified residue	261	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	513 – 574	62	VNSYG…WDLIT → GTAPSQLPWRWRLC in isoform FOXJ2.S.		VSP_001544
	Natural variant	229	1	P → R: dbSNP rs35642012.		VAR_049162
	Natural variant	310	1	P → S: dbSNP rs2277415.		VAR_021842

Sequences

	Sequence		Length	Mass (Da)
	Isoform FOXJ2.L (FHX.L) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 258120EDAE4B11EB	FASTA	574	62,395
	10 20 30 40 50 60 MASDLESSLT SIDWLPQLTL RATIEKLGSA SQAGPPGSSR KCSPGSPTDP NATLSKDEAA 70 80 90 100 110 120 VHQDGKPRYS YATLITYAIN SSPAKKMTLS EIYRWICDNF PYYKNAGIGW KNSIRHNLSL 130 140 150 160 170 180 NKCFRKVPRP RDDPGKGSYW TIDTCPDISR KRRHPPDDDL SQDSPEQEAS KSPRGGVAGS 190 200 210 220 230 240 GEASLPPEGN PQMSLQSPTS IASYSQGTGS VDGGAVAAGA SGRESAEGPP PLYNTNHDFK 250 260 270 280 290 300 FSYSEINFQD LSWSFRNLYK SMLEKSSSSS QHGFSSLLGD IPPSNNYYMY QQQQPPPPQQ 310 320 330 340 350 360 QQQQQQPPQP PPQQSQPQQQ QAPAQGPSAV GGAPPLHTPS TDGCTPPGGK QAGAEGYGPP 370 380 390 400 410 420 PVMAMHPPPL QHGGYHPHQH HPHSHPAQQP PPPQPQAQGQ APINNTGFAF PSDWCSNIDS 430 440 450 460 470 480 LKESFKMVNR LNWSSIEQSQ FSELMESLRQ AEQKNWTLDQ HHIANLCDSL NHFLTQTGHV 490 500 510 520 530 540 PPQGGTHRPP APARIADSCA LTSGKQESAM SQVNSYGHPQ APHLYPGPSP MYPIPTQDSA 550 560 570 GYNRPAHHMV PRPSVPPPGA NEEIPDDFDW DLIT
	Isoform FOXJ2.S (FHX.S). Checksum: 666CFE383030805D	FASTA	526	57,220
	10 20 30 40 50 60 MASDLESSLT SIDWLPQLTL RATIEKLGSA SQAGPPGSSR KCSPGSPTDP NATLSKDEAA 70 80 90 100 110 120 VHQDGKPRYS YATLITYAIN SSPAKKMTLS EIYRWICDNF PYYKNAGIGW KNSIRHNLSL 130 140 150 160 170 180 NKCFRKVPRP RDDPGKGSYW TIDTCPDISR KRRHPPDDDL SQDSPEQEAS KSPRGGVAGS 190 200 210 220 230 240 GEASLPPEGN PQMSLQSPTS IASYSQGTGS VDGGAVAAGA SGRESAEGPP PLYNTNHDFK 250 260 270 280 290 300 FSYSEINFQD LSWSFRNLYK SMLEKSSSSS QHGFSSLLGD IPPSNNYYMY QQQQPPPPQQ 310 320 330 340 350 360 QQQQQQPPQP PPQQSQPQQQ QAPAQGPSAV GGAPPLHTPS TDGCTPPGGK QAGAEGYGPP 370 380 390 400 410 420 PVMAMHPPPL QHGGYHPHQH HPHSHPAQQP PPPQPQAQGQ APINNTGFAF PSDWCSNIDS 430 440 450 460 470 480 LKESFKMVNR LNWSSIEQSQ FSELMESLRQ AEQKNWTLDQ HHIANLCDSL NHFLTQTGHV 490 500 510 520 PPQGGTHRPP APARIADSCA LTSGKQESAM SQGTAPSQLP WRWRLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FHX, a novel fork head factor with a dual DNA binding specificity." Perez-Sanchez C., Gomez-Ferreria M.A., de la Fuente C.A., Granadino B., Velasco G., Esteban A., Rey-Campos J. J. Biol. Chem. 275:12909-12916(2000) [PubMed: 10777590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FOXJ2.L).
[2]	"FHX.L and FHX.S, two isoforms of the human fork-head factor FHX (FOXJ2) with differential activity." Perez-Sanchez C., de la Fuente C.A., Gomez-Ferreria M.A., Granadino B., Rey-Campos J. J. Mol. Biol. 301:795-806(2000) [PubMed: 10966786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FOXJ2.S).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM FOXJ2.L). Tissue: Lung.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 458-574 (ISOFORM FOXJ2.L). Tissue: Melanoma.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-161, MASS SPECTROMETRY.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-259 AND SER-261, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF155132 mRNA. Translation: AAF65927.1. AF155133 mRNA. Translation: AAK49016.1. BC126396 mRNA. Translation: AAI26397.1. BC136305 mRNA. Translation: AAI36306.1. AL161978 mRNA. Translation: CAB82315.1.
IPI	IPI00000781. IPI00221299.
PIR	T47161.
RefSeq	NP_060886.1.
UniGene	Hs.120844
3D structure databases
HSSP	HSSP built from PDB template 1D5V based on UniProtKB Q99958.
ModBase	Search...
PTM databases
PhosphoSite	Q9P0K8.
Proteomic databases
PRIDE	Q9P0K8.
Genome annotation databases
Ensembl	ENSG00000065970. Homo sapiens. [Contig view]
GeneID	55810.
KEGG	hsa:55810.
Organism-specific databases
GeneCards	GC12P008079.
H-InvDB	HIX0010401.
HGNC	HGNC:24818. FOXJ2.
HPA	HPA008723.
PharmGKB	PA134982817.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9P0K8.
HOVERGEN	Q9P0K8.
OMA	Q9P0K8. RHPPDDD.
Gene expression databases
ArrayExpress	Q9P0K8.
Bgee	Q9P0K8.
CleanEx	HS_FOXJ2.
GermOnline	ENSG00000065970. Homo sapiens.
Family and domain databases
InterPro	IPR001766. TF_fork_head. IPR018122. TF_fork_head_CS. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR11829. Fork_box_protein. 1 hit.
Pfam	PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
ProDom	PD000425. TF_Fork_head. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00339. FH. 1 hit. [Graphical view]
PROSITE	PS00657. FORK_HEAD_1. False negative. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	60973.

Entry information

Entry name

FOXJ2_HUMAN

Accession

Primary (citable) accession number: Q9P0K8
Secondary accession number(s): A0AVK4 , B2RMP3, Q96PS9, Q9NSN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)