Q9P0K8 (FOXJ2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Forkhead box protein J2

Alternative name(s):
Fork head homologous X

Gene names

Name:	FOXJ2
Synonyms:	FHX

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator. Able to bind to two different type of DNA binding sites. Isoform FOXJ2.L behaves as a more potent transactivator than FOXJ2.S.
Subcellular location	Nucleus.
Tissue specificity	Widely expressed.
Sequence similarities	Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryo development Inferred from Biological aspect of Ancestor. Source: RefGenome organ development Inferred from Biological aspect of Ancestor. Source: RefGenome pattern specification process Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of transcription, DNA-dependent Inferred from direct assay Ref.1Ref.2. Source: UniProtKB regulation of sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome tissue development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	nucleolus Inferred from direct assay. Source: HPA nucleus Inferred by curator Ref.1. Source: UniProtKB transcription factor complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	DNA binding, bending Inferred from Biological aspect of Ancestor. Source: RefGenome RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome double-stranded DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein domain specific binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific DNA binding Inferred from direct assay Ref.1. Source: UniProtKB transcription factor binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform FOXJ2.L (identifier: Q9P0K8-1)

Also known as: FHX.L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform FOXJ2.S (identifier: Q9P0K8-2)

Also known as: FHX.S;

The sequence of this isoform differs from the canonical sequence as follows:
     513-574: VNSYGHPQAP...PDDFDWDLIT → GTAPSQLPWRWRLC

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.8
	Chain	2 – 574	573	Forkhead box protein J2		PRO_0000091853
Regions
	DNA binding	66 – 143	78	Fork-head
	Compositional bias	266 – 270	5	Poly-Ser
	Compositional bias	291 – 294	4	Poly-Gln
	Compositional bias	295 – 298	4	Poly-Pro
	Compositional bias	299 – 306	8	Poly-Gln
	Compositional bias	313 – 321	9	Poly-Gln
	Compositional bias	390 – 395	6	Poly-Pro
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.8
	Modified residue	3	1	Phosphoserine Ref.8
	Modified residue	161	1	Phosphoserine Ref.5
	Modified residue	172	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	513 – 574	62	VNSYG…WDLIT → GTAPSQLPWRWRLC in isoform FOXJ2.S.		VSP_001544
	Natural variant	229	1	P → R. Corresponds to variant rs35642012 [ dbSNP | Ensembl ].		VAR_049162
	Natural variant	310	1	P → S. Corresponds to variant rs2277415 [ dbSNP | Ensembl ].		VAR_021842

Sequences

	Sequence		Length	Mass (Da)
	Isoform FOXJ2.L (FHX.L) [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 258120EDAE4B11EB	FASTA	574	62,395
	10 20 30 40 50 60 MASDLESSLT SIDWLPQLTL RATIEKLGSA SQAGPPGSSR KCSPGSPTDP NATLSKDEAA 70 80 90 100 110 120 VHQDGKPRYS YATLITYAIN SSPAKKMTLS EIYRWICDNF PYYKNAGIGW KNSIRHNLSL 130 140 150 160 170 180 NKCFRKVPRP RDDPGKGSYW TIDTCPDISR KRRHPPDDDL SQDSPEQEAS KSPRGGVAGS 190 200 210 220 230 240 GEASLPPEGN PQMSLQSPTS IASYSQGTGS VDGGAVAAGA SGRESAEGPP PLYNTNHDFK 250 260 270 280 290 300 FSYSEINFQD LSWSFRNLYK SMLEKSSSSS QHGFSSLLGD IPPSNNYYMY QQQQPPPPQQ 310 320 330 340 350 360 QQQQQQPPQP PPQQSQPQQQ QAPAQGPSAV GGAPPLHTPS TDGCTPPGGK QAGAEGYGPP 370 380 390 400 410 420 PVMAMHPPPL QHGGYHPHQH HPHSHPAQQP PPPQPQAQGQ APINNTGFAF PSDWCSNIDS 430 440 450 460 470 480 LKESFKMVNR LNWSSIEQSQ FSELMESLRQ AEQKNWTLDQ HHIANLCDSL NHFLTQTGHV 490 500 510 520 530 540 PPQGGTHRPP APARIADSCA LTSGKQESAM SQVNSYGHPQ APHLYPGPSP MYPIPTQDSA 550 560 570 GYNRPAHHMV PRPSVPPPGA NEEIPDDFDW DLIT
	Isoform FOXJ2.S (FHX.S) [UniParc]. Checksum: 666CFE383030805D	FASTA	526	57,220
	10 20 30 40 50 60 MASDLESSLT SIDWLPQLTL RATIEKLGSA SQAGPPGSSR KCSPGSPTDP NATLSKDEAA 70 80 90 100 110 120 VHQDGKPRYS YATLITYAIN SSPAKKMTLS EIYRWICDNF PYYKNAGIGW KNSIRHNLSL 130 140 150 160 170 180 NKCFRKVPRP RDDPGKGSYW TIDTCPDISR KRRHPPDDDL SQDSPEQEAS KSPRGGVAGS 190 200 210 220 230 240 GEASLPPEGN PQMSLQSPTS IASYSQGTGS VDGGAVAAGA SGRESAEGPP PLYNTNHDFK 250 260 270 280 290 300 FSYSEINFQD LSWSFRNLYK SMLEKSSSSS QHGFSSLLGD IPPSNNYYMY QQQQPPPPQQ 310 320 330 340 350 360 QQQQQQPPQP PPQQSQPQQQ QAPAQGPSAV GGAPPLHTPS TDGCTPPGGK QAGAEGYGPP 370 380 390 400 410 420 PVMAMHPPPL QHGGYHPHQH HPHSHPAQQP PPPQPQAQGQ APINNTGFAF PSDWCSNIDS 430 440 450 460 470 480 LKESFKMVNR LNWSSIEQSQ FSELMESLRQ AEQKNWTLDQ HHIANLCDSL NHFLTQTGHV 490 500 510 520 PPQGGTHRPP APARIADSCA LTSGKQESAM SQGTAPSQLP WRWRLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FHX, a novel fork head factor with a dual DNA binding specificity." Perez-Sanchez C., Gomez-Ferreria M.A., de la Fuente C.A., Granadino B., Velasco G., Esteban A., Rey-Campos J. J. Biol. Chem. 275:12909-12916(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FOXJ2.L).
[2]	"FHX.L and FHX.S, two isoforms of the human fork-head factor FHX (FOXJ2) with differential activity." Perez-Sanchez C., de la Fuente C.A., Gomez-Ferreria M.A., Granadino B., Rey-Campos J. J. Mol. Biol. 301:795-806(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM FOXJ2.S).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM FOXJ2.L). Tissue: Lung.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 458-574 (ISOFORM FOXJ2.L). Tissue: Melanoma.
[5]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-161, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF155132 mRNA. Translation: AAF65927.1. AF155133 mRNA. Translation: AAK49016.1. BC126396 mRNA. Translation: AAI26397.1. BC136305 mRNA. Translation: AAI36306.1. AL161978 mRNA. Translation: CAB82315.1.
IPI	IPI00000781. IPI00221299.
PIR	T47161.
RefSeq	NP_060886.1. NM_018416.2.
UniGene	Hs.120844.
3D structure databases
ProteinModelPortal	Q9P0K8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P0K8. 1 interaction.
PTM databases
PhosphoSite	Q9P0K8.
Polymorphism databases
DMDM	13626933.
Proteomic databases
PaxDb	Q9P0K8.
PRIDE	Q9P0K8.
Protocols and materials databases
DNASU	55810.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000162391; ENSP00000162391; ENSG00000065970. ENST00000428177; ENSP00000403411; ENSG00000065970.
GeneID	55810.
KEGG	hsa:55810.
UCSC	uc001qtt.1. human. uc001qtu.3. human.
Organism-specific databases
CTD	55810.
GeneCards	GC12P008185.
HGNC	HGNC:24818. FOXJ2.
HPA	HPA008723.
neXtProt	NX_Q9P0K8.
PharmGKB	PA134982817.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5025.
HOGENOM	HOG000231184.
HOVERGEN	HBG051648.
InParanoid	Q9P0K8.
KO	K09403.
OMA	DLSQDSP.
OrthoDB	EOG473PR7.
PhylomeDB	Q9P0K8.
Gene expression databases
Bgee	Q9P0K8.
CleanEx	HS_FOXJ2.
Genevestigator	Q9P0K8.
GermOnline	ENSG00000065970. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR001766. TF_fork_head. IPR018122. TF_fork_head_CS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
SMART	SM00339. FH. 1 hit. [Graphical view]
PROSITE	PS00657. FORK_HEAD_1. False negative. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55810.
NextBio	60973.

Entry information

Entry name

FOXJ2_HUMAN

Accession

Primary (citable) accession number: Q9P0K8
Secondary accession number(s): A0AVK4 , B2RMP3, Q96PS9, Q9NSN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.