Ankycorbin - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Ankycorbin
Alternative name(s):
    Ankyrin repeat and coiled-coil structure-containing protein
    Retinoic acid-induced protein 14
    Novel retinal pigment epithelial cell protein

Gene names

Name:	RAI14
Synonyms:	KIAA1334, NORPEG

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Subcellular location	Cytoplasm › cell cortex By similarity. Cytoplasm › cytoskeleton By similarity. Note: Associated with the cortical actin cytoskeleton structures in terminal web and cell-cell adhesion sites and stress fibers By similarity.
Tissue specificity	Highly expressed in placenta, muscle, kidney and testis. Moderately expressed in heart, brain, lung, liver and intestine. Isoform 2 is widely expressed and expressed in fetal and adult testes, and spermatozoa. Ref.1 Ref.2 Ref.7
Induction	Up-regulated by all-trans-retinoic acid in human retinal pigment epithelial cells (ARPE-19). Ref.1
Miscellaneous	Doesn't bind directly to F-actin By similarity.
Sequence similarities	Contains 7 ANK repeats.

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Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Coiled coil Repeat
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
YWHAB	P31946	1	EBI-1023749,EBI-359815
YWHAG	P61981	1	EBI-1023749,EBI-359832
YWHAQ	P27348	1	EBI-1023749,EBI-359854
YWHAZ	P63104	1	EBI-1023749,EBI-347088

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9P0K7-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9P0K7-2) Also known as: sNORPEG; The sequence of this isoform differs from the canonical sequence as follows: 1-12: MKSLKAKFRKSD → MQPTYLPWLSAKEKK

Isoform 3 (identifier: Q9P0K7-3) The sequence of this isoform differs from the canonical sequence as follows: 1-12: MKSLKAKFRKSD → MEAK

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 980

980

Ankycorbin

PRO_0000239630

Regions

Repeat

18 – 51

34

ANK 1

Repeat

52 – 81

30

ANK 2

Repeat

85 – 114

30

ANK 3

Repeat

118 – 147

30

ANK 4

Repeat

151 – 180

30

ANK 5

Repeat

184 – 213

30

ANK 6

Repeat

217 – 247

31

ANK 7

Coiled coil

349 – 374

26

Potential

Coiled coil

425 – 947

523

Potential

Amino acid modifications

Modified residue

249

1

Phosphothreonine Ref.8

Modified residue

293

1

Phosphoserine Ref.9

Modified residue

358

1

Phosphoserine Ref.8

Natural variations

Alternative sequence

1 – 12

12

MKSLK…FRKSD → MQPTYLPWLSAKEKK in isoform 2.

VSP_019248

Alternative sequence

1 – 12

12

MKSLK…FRKSD → MEAK in isoform 3.

VSP_019249

Natural variant

44

1

A → T: dbSNP rs17521570.

VAR_026673

Natural variant

45

1

S → N: dbSNP rs35941954.

VAR_055517

Natural variant

499

1

V → L: dbSNP rs10472941.

VAR_055518

Natural variant

870

1

A → S: dbSNP rs1048944.

VAR_055519

Experimental info

Sequence conflict

104

1

K → R in AAF44722. Ref.1

Sequence conflict

272

1

K → T in AAF44722. Ref.1

Sequence conflict

272

1

K → T in AAQ63889. Ref.3

Sequence conflict

434

1

L → P in AAQ63889. Ref.3

Sequence conflict

491

1

K → Q in AAQ63889. Ref.3

Sequence conflict

602

1

Q → P in AAQ63889. Ref.3

Sequence conflict

607

1

E → G in AAQ63889. Ref.3

Sequence conflict

610

1

K → E in AAQ63889. Ref.3

Sequence conflict

636

1

R → G in CAB43236. Ref.6

Sequence conflict

766

1

A → L in AAQ63889. Ref.3

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 13, 2006. Version 2. Checksum: DFCF4B7913B30B69 Show »	FASTA	980	110,041
10 20 30 40 50 60 MKSLKAKFRK SDTNEWNKND DRLLQAVENG DAEKVASLLG KKGASATKHD SEGKTAFHLA 70 80 90 100 110 120 AAKGHVECLR VMITHGVDVT AQDTTGHSAL HLAAKNSHHE CIRKLLQSKC PAESVDSSGK 130 140 150 160 170 180 TALHYAAAQG CLQAVQILCE HKSPINLKDL DGNIPLLLAV QNGHSEICHF LLDHGADVNS 190 200 210 220 230 240 RNKSGRTALM LACEIGSSNA VEALIKKGAD LNLVDSLGYN ALHYSKLSEN AGIQSLLLSK 250 260 270 280 290 300 ISQDADLKTP TKPKQHDQVS KISSERSGTP KKRKAPPPPI SPTQLSDVSS PRSITSTPLS 310 320 330 340 350 360 GKESVFFAEP PFKAEISSIR ENKDRLSDST TGADSLLDIS SEADQQDLLS LLQAKVASLT 370 380 390 400 410 420 LHNKELQDKL QAKSPKEAEA DLSFDSYHST QTDLGPSLGK PGETSPPDSK SSPSVLIHSL 430 440 450 460 470 480 GKSTTDNDVR IQQLQEILQD LQKRLESSEA ERKQLQVELQ SRRAELVCLN NTEISENSSD 490 500 510 520 530 540 LSQKLKETQS KYEEAMKEVL SVQKQMKLGL VSPESMDNYS HFHELRVTEE EINVLKQDLQ 550 560 570 580 590 600 NALEESERNK EKVRELEEKL VEREKGTVIK PPVEEYEEMK SSYCSVIENM NKEKAFLFEK 610 620 630 640 650 660 YQEAQEEIMK LKDTLKSQMT QEASDEAEDM KEAMNRMIDE LNKQVSELSQ LYKEAQAELE 670 680 690 700 710 720 DYRKRKSLED VTAEYIHKAE HEKLMQLTNV SRAKAEDALS EMKSQYSKVL NELTQLKQLV 730 740 750 760 770 780 DAQKENSVSI TEHLQVITTL RTAAKEMEEK ISNLKEHLAS KEVEVAKLEK QLLEEKAAMT 790 800 810 820 830 840 DAMVPRSSYE KLQSSLESEV SVLASKLKES VKEKEKVHSE VVQIRSEVSQ VKREKENIQT 850 860 870 880 890 900 LLKSKEQEVN ELLQKFQQAQ EELAEMKRYA ESSSKLEEDK DKKINEMSKE VTKLKEALNS 910 920 930 940 950 960 LSQLSYSTSS SKRQSQQLEA LQQQVKQLQN QLAECKKQHQ EVISVYRMHL LYAVQGQMDE 970 980 DVQKVLKQIL TMCKNQSQKK « Hide
Isoform 2 (sNORPEG). Checksum: 9B18E2F9DBFF5A6D Show »	FASTA	983	110,423
10 20 30 40 50 60 MQPTYLPWLS AKEKKTNEWN KNDDRLLQAV ENGDAEKVAS LLGKKGASAT KHDSEGKTAF 70 80 90 100 110 120 HLAAAKGHVE CLRVMITHGV DVTAQDTTGH SALHLAAKNS HHECIRKLLQ SKCPAESVDS 130 140 150 160 170 180 SGKTALHYAA AQGCLQAVQI LCEHKSPINL KDLDGNIPLL LAVQNGHSEI CHFLLDHGAD 190 200 210 220 230 240 VNSRNKSGRT ALMLACEIGS SNAVEALIKK GADLNLVDSL GYNALHYSKL SENAGIQSLL 250 260 270 280 290 300 LSKISQDADL KTPTKPKQHD QVSKISSERS GTPKKRKAPP PPISPTQLSD VSSPRSITST 310 320 330 340 350 360 PLSGKESVFF AEPPFKAEIS SIRENKDRLS DSTTGADSLL DISSEADQQD LLSLLQAKVA 370 380 390 400 410 420 SLTLHNKELQ DKLQAKSPKE AEADLSFDSY HSTQTDLGPS LGKPGETSPP DSKSSPSVLI 430 440 450 460 470 480 HSLGKSTTDN DVRIQQLQEI LQDLQKRLES SEAERKQLQV ELQSRRAELV CLNNTEISEN 490 500 510 520 530 540 SSDLSQKLKE TQSKYEEAMK EVLSVQKQMK LGLVSPESMD NYSHFHELRV TEEEINVLKQ 550 560 570 580 590 600 DLQNALEESE RNKEKVRELE EKLVEREKGT VIKPPVEEYE EMKSSYCSVI ENMNKEKAFL 610 620 630 640 650 660 FEKYQEAQEE IMKLKDTLKS QMTQEASDEA EDMKEAMNRM IDELNKQVSE LSQLYKEAQA 670 680 690 700 710 720 ELEDYRKRKS LEDVTAEYIH KAEHEKLMQL TNVSRAKAED ALSEMKSQYS KVLNELTQLK 730 740 750 760 770 780 QLVDAQKENS VSITEHLQVI TTLRTAAKEM EEKISNLKEH LASKEVEVAK LEKQLLEEKA 790 800 810 820 830 840 AMTDAMVPRS SYEKLQSSLE SEVSVLASKL KESVKEKEKV HSEVVQIRSE VSQVKREKEN 850 860 870 880 890 900 IQTLLKSKEQ EVNELLQKFQ QAQEELAEMK RYAESSSKLE EDKDKKINEM SKEVTKLKEA 910 920 930 940 950 960 LNSLSQLSYS TSSSKRQSQQ LEALQQQVKQ LQNQLAECKK QHQEVISVYR MHLLYAVQGQ 970 980 MDEDVQKVLK QILTMCKNQS QKK « Hide
Isoform 3. Checksum: 5862375423E1C605 Show »	FASTA	972	109,080
10 20 30 40 50 60 MEAKTNEWNK NDDRLLQAVE NGDAEKVASL LGKKGASATK HDSEGKTAFH LAAAKGHVEC 70 80 90 100 110 120 LRVMITHGVD VTAQDTTGHS ALHLAAKNSH HECIRKLLQS KCPAESVDSS GKTALHYAAA 130 140 150 160 170 180 QGCLQAVQIL CEHKSPINLK DLDGNIPLLL AVQNGHSEIC HFLLDHGADV NSRNKSGRTA 190 200 210 220 230 240 LMLACEIGSS NAVEALIKKG ADLNLVDSLG YNALHYSKLS ENAGIQSLLL SKISQDADLK 250 260 270 280 290 300 TPTKPKQHDQ VSKISSERSG TPKKRKAPPP PISPTQLSDV SSPRSITSTP LSGKESVFFA 310 320 330 340 350 360 EPPFKAEISS IRENKDRLSD STTGADSLLD ISSEADQQDL LSLLQAKVAS LTLHNKELQD 370 380 390 400 410 420 KLQAKSPKEA EADLSFDSYH STQTDLGPSL GKPGETSPPD SKSSPSVLIH SLGKSTTDND 430 440 450 460 470 480 VRIQQLQEIL QDLQKRLESS EAERKQLQVE LQSRRAELVC LNNTEISENS SDLSQKLKET 490 500 510 520 530 540 QSKYEEAMKE VLSVQKQMKL GLVSPESMDN YSHFHELRVT EEEINVLKQD LQNALEESER 550 560 570 580 590 600 NKEKVRELEE KLVEREKGTV IKPPVEEYEE MKSSYCSVIE NMNKEKAFLF EKYQEAQEEI 610 620 630 640 650 660 MKLKDTLKSQ MTQEASDEAE DMKEAMNRMI DELNKQVSEL SQLYKEAQAE LEDYRKRKSL 670 680 690 700 710 720 EDVTAEYIHK AEHEKLMQLT NVSRAKAEDA LSEMKSQYSK VLNELTQLKQ LVDAQKENSV 730 740 750 760 770 780 SITEHLQVIT TLRTAAKEME EKISNLKEHL ASKEVEVAKL EKQLLEEKAA MTDAMVPRSS 790 800 810 820 830 840 YEKLQSSLES EVSVLASKLK ESVKEKEKVH SEVVQIRSEV SQVKREKENI QTLLKSKEQE 850 860 870 880 890 900 VNELLQKFQQ AQEELAEMKR YAESSSKLEE DKDKKINEMS KEVTKLKEAL NSLSQLSYST 910 920 930 940 950 960 SSSKRQSQQL EALQQQVKQL QNQLAECKKQ HQEVISVYRM HLLYAVQGQM DEDVQKVLKQ 970 ILTMCKNQSQ KK « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization and developmental expression of NORPEG, a novel gene induced by retinoic acid." Kutty R.K., Kutty G., Samuel W., Duncan T., Bridges C.C., El-Sherbeeny A., Nagineni C.N., Smith S.B., Wiggert B. J. Biol. Chem. 276:2831-2840(2001) [PubMed: 11042181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY. Tissue: Retinal pigment epithelium.
[2]	"Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes." Yuan W., Zheng Y., Huo R., Lu L., Huang X.-Y., Yin L.-L., Li J.-M., Zhou Z.-M., Sha J.-H. Asian J. Androl. 7:277-288(2005) [PubMed: 16110356] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY. Tissue: Testis.
[3]	Sha J.H., Zhou Z.M., Li J.M. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Testis.
[4]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-870. Tissue: Skin.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 636-980. Tissue: Brain.
[7]	"Ankycorbin: a novel actin cytoskeleton-associated protein." Peng Y.-F., Mandai K., Sakisaka T., Okabe N., Yamamoto Y., Yokoyama S., Mizoguchi A., Shiozaki H., Monden M., Takai Y. Genes Cells 5:1001-1008(2000) [PubMed: 11168586] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-249 AND SER-358, MASS SPECTROMETRY.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-293, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF155135 mRNA. Translation: AAF44722.1. AY317139 mRNA. Translation: AAP84319.1. AY354204 mRNA. Translation: AAQ63889.2. AB037755 mRNA. Translation: BAA92572.1. Different initiation. BC052988 mRNA. Translation: AAH52988.1. AL050011 mRNA. Translation: CAB43236.2. Different initiation.
IPI	IPI00292953. IPI00759532. IPI00759780.
PIR	T08700.
RefSeq	NP_001138992.1. NP_001138993.1. NP_001138995.1. NP_056392.2.
UniGene	Hs.431400
3D structure databases
HSSP	HSSP built from PDB template 1IHB based on UniProtKB P42773.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P0K7. 7 interactions.
PTM databases
PhosphoSite	Q9P0K7.
Proteomic databases
PRIDE	Q9P0K7.
Genome annotation databases
Ensembl	ENSG00000039560. Homo sapiens. [Contig view]
GeneID	26064.
KEGG	hsa:26064.
NMPDR	fig\|9606.3.peg.25121.
Organism-specific databases
GeneCards	GC05P034692.
H-InvDB	HIX0004796.
HGNC	HGNC:14873. RAI14.
MIM	606586. gene.
PharmGKB	PA34189.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9P0K7.
HOVERGEN	Q9P0K7.
OMA	Q9P0K7. NEWNKND.
Gene expression databases
ArrayExpress	Q9P0K7.
Bgee	Q9P0K7.
CleanEx	HS_RAI14.
Family and domain databases
InterPro	IPR002110. ANK. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 7 hits. [Graphical view]
SMART	SM00248. ANK. 6 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 5 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	47969.
SOURCE	Search...

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Entry information

Entry name

RAI14_HUMAN

Accession

Primary (citable) accession number: Q9P0K7
Secondary accession number(s): Q6V1W9

Q9Y3T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 13, 2006
Last modified:	June 16, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents