ID   ADA22_HUMAN    STANDARD;      PRT;   906 AA.
AC   Q9P0K1; O75075; O75076; Q9P0K2; Q9UIA1; Q9UKK2;
DT   16-OCT-2001 (Rel. 40, Created)
DT   16-OCT-2001 (Rel. 40, Last sequence update)
DT   10-MAY-2005 (Rel. 47, Last annotation update)
DE   ADAM 22 precursor (A disintegrin and metalloproteinase domain 22)
DE   (Metalloproteinase-like, disintegrin-like, and cysteine-rich protein
DE   2) (Metalloproteinase-disintegrin ADAM22-3).
GN   Name=ADAM22; Synonyms=MDC2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2).
RX   MEDLINE=20504287; PubMed=11050470;
RA   Harada T., Nishie A., Torigoe K., Ikezaki K., Shono T., Maehara Y.,
RA   Kuwano M., Wada M.;
RT   "The specific expression of three novel splice variant forms of human
RT   metalloprotease-like disintegrin-like cysteine-rich protein 2 gene in
RT   brain tissues and gliomas.";
RL   Jpn. J. Cancer Res. 91:1001-1006(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE (ISOFORMS 3 AND 4).
RC   TISSUE=Brain;
RX   MEDLINE=98359734; PubMed=9693107;
RA   Sagane K., Ohya Y., Hasegawa Y., Tanaka I.;
RT   "Metalloproteinase-like, disintegrin-like, cysteine-rich proteins MDC2
RT   and MDC3: novel human cellular disintegrins highly expressed in the
RT   brain.";
RL   Biochem. J. 334:93-98(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE (ISOFORM 5), AND VARIANT ARG-81.
RC   TISSUE=Brain;
RA   Wada M., Torigoe K., Harada T., Kuwano M.;
RT   "Isolation and tissue specific expression of novel ADAM family from
RT   7q21.1 region.";
RL   Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE OF 40-906 (ISOFORM 1), AND VARIANT ARG-81.
RC   TISSUE=Cerebellum;
RX   MEDLINE=99453762; PubMed=10524237; DOI=10.1016/S0378-1119(99)00302-9;
RA   Poindexter K., Nelson N., DuBose R.F., Black R.A., Cerretti D.P.;
RT   "The identification of seven metalloproteinase-disintegrin (ADAM)
RT   genes from genomic libraries.";
RL   Gene 237:61-70(1999).
RN   [5]
RP   SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
RX   PubMed=14759258; DOI=10.1186/gb-2004-5-2-r8;
RA   Hillman R.T., Green R.E., Brenner S.E.;
RT   "An unappreciated role for RNA surveillance.";
RL   Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004).
CC   -!- FUNCTION: Probable ligand for integrin in the brain. This is a non
CC       catalytic metalloprotease-like protein.
CC   -!- SUBCELLULAR LOCATION: Type I membrane protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=5;
CC       Name=1; Synonyms=Epsilon;
CC         IsoId=Q9P0K1-1; Sequence=Displayed;
CC       Name=2; Synonyms=Delta;
CC         IsoId=Q9P0K1-2; Sequence=VSP_005482, VSP_005484;
CC         Note=May be produced at very low levels due to a premature stop
CC         codon in the mRNA, leading to nonsense-mediated mRNA decay;
CC       Name=3; Synonyms=Alpha;
CC         IsoId=Q9P0K1-3; Sequence=VSP_005483;
CC       Name=4; Synonyms=Beta;
CC         IsoId=Q9P0K1-4; Sequence=VSP_005482, VSP_005483;
CC       Name=5;
CC         IsoId=Q9P0K1-5; Sequence=VSP_005482;
CC   -!- TISSUE SPECIFICITY: Highly expressed in the brain. Detected
CC       slighly or not at all in other tissues.
CC   -!- PTM: The precursor is cleaved by a furin endopeptidase (By
CC       similarity).
CC   -!- SIMILARITY: Contains 1 disintegrin domain.
CC   -!- SIMILARITY: Contains 1 EGF-like domain.
CC   -!- SIMILARITY: Contains 1 peptidase M12B domain.
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DR   EMBL; AF155382; AAF73289.1; -; mRNA.
DR   EMBL; AF155381; AAF73288.1; -; mRNA.
DR   EMBL; AB009671; BAA32349.1; -; mRNA.
DR   EMBL; AB009671; BAA32350.1; -; mRNA.
DR   EMBL; AF073291; AAF22476.2; -; mRNA.
DR   EMBL; AF158637; AAD55251.1; -; mRNA.
DR   HSSP; P18619; 1FVL.
DR   MEROPS; M12.978; -.
DR   Ensembl; ENSG00000008277; Homo sapiens.
DR   HGNC; HGNC:201; ADAM22.
DR   MIM; 603709; -.
DR   GO; GO:0016021; C:integral to membrane; NAS.
DR   GO; GO:0005178; F:integrin binding; NAS.
DR   GO; GO:0007417; P:central nervous system development; TAS.
DR   GO; GO:0007162; P:negative regulation of cell adhesion; NAS.
DR   InterPro; IPR006586; ADAM_cysteine.
DR   InterPro; IPR001762; Disintegrin.
DR   InterPro; IPR000742; EGF_2.
DR   InterPro; IPR006209; EGF_like.
DR   InterPro; IPR006210; IEGF.
DR   InterPro; IPR006025; Pept_M_Zn_BS.
DR   InterPro; IPR001590; Peptidase_M12B.
DR   InterPro; IPR002870; Peptidase_M12B_N.
DR   Pfam; PF00200; Disintegrin; 1.
DR   Pfam; PF01562; Pep_M12B_propep; 1.
DR   Pfam; PF01421; Reprolysin; 1.
DR   PRINTS; PR00289; DISINTEGRIN.
DR   ProDom; PD000664; Disintegrin; 1.
DR   SMART; SM00608; ACR; 1.
DR   SMART; SM00050; DISIN; 1.
DR   SMART; SM00181; EGF; 1.
DR   PROSITE; PS50215; ADAM_MEPRO; 1.
DR   PROSITE; PS00427; DISINTEGRIN_1; FALSE_NEG.
DR   PROSITE; PS50214; DISINTEGRIN_2; 1.
DR   PROSITE; PS00022; EGF_1; 1.
DR   PROSITE; PS01186; EGF_2; FALSE_NEG.
DR   PROSITE; PS50026; EGF_3; FALSE_NEG.
DR   PROSITE; PS00142; ZINC_PROTEASE; FALSE_NEG.
KW   Alternative splicing; EGF-like domain; Glycoprotein; Polymorphism;
KW   Signal; Transmembrane.
FT   SIGNAL        1     25       Potential.
FT   PROPEP       26    222       By similarity.
FT                                /FTId=PRO_0000029112.
FT   CHAIN       223    906       ADAM 22.
FT                                /FTId=PRO_0000029113.
FT   TOPO_DOM    223    736       Extracellular (Potential).
FT   TRANSMEM    737    757       Potential.
FT   TOPO_DOM    758    906       Cytoplasmic (Potential).
FT   DOMAIN      239    438       Peptidase M12B.
FT   DOMAIN      444    531       Disintegrin.
FT   DOMAIN      675    712       EGF-like.
FT   COMPBIAS    532    678       Cys-rich.
FT   CARBOHYD    175    175       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    519    519       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    634    634       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    675    675       N-linked (GlcNAc...) (Potential).
FT   DISULFID    349    433       By similarity.
FT   DISULFID    503    516       Potential.
FT   DISULFID    679    694       By similarity.
FT   DISULFID    688    700       By similarity.
FT   DISULFID    702    711       By similarity.
FT   VARSPLIC    768    803       Missing (in isoform 2, isoform 4 and
FT                                isoform 5).
FT                                /FTId=VSP_005482.
FT   VARSPLIC    859    859       E -> EYLNPWFKRDYNVAKWVEDVNKNTEEPYFR (in
FT                                isoform 2).
FT                                /FTId=VSP_005484.
FT   VARSPLIC    860    906       Missing (in isoform 3 and isoform 4).
FT                                /FTId=VSP_005483.
FT   VARIANT      81     81       P -> R (in dbSNP:2279542).
FT                                /FTId=VAR_020057.
SQ   SEQUENCE   906 AA;  100433 MW;  265ECCD0FA6C088B CRC64;
     MQAAVAVSVP FLLLCVLGTC PPARCGQAGD ASLMELEKRK ENRFVERQSI VPLRLIYRSG
     GEDESRHDAL DTRVRGDLGG PQLTHVDQAS FQVDAFGTSF ILDVVLNHDL LSSEYIERHI
     EHGGKTVEVK GGEHCYYQGH IRGNPDSFVA LSTCHGLHGM FYDGNHTYLI EPEENDTTQE
     DFHFHSVYKS RLFEFSLDDL PSEFQQVNIT PSKFILKPRP KRSKRQLRRY PRNVEEETKY
     IELMIVNDHL MFKKHRLSVV HTNTYAKSVV NMADLIYKDQ LKTRIVLVAM ETWATDNKFA
     ISENPLITLR EFMKYRRDFI KEKSDAVHLF SGSQFESSRS GAAYIGGICS LLKGGGVNEF
     GKTDLMAVTL AQSLAHNIGI ISDKRKLASG ECKCEDTWSG CIMGDTGYYL PKKFTQCNIE
     EYHDFLNSGG GACLFNKPSK LLDPPECGNG FIETGEECDC GTPAECVLEG AECCKKCTLT
     QDSQCSDGLC CKKCKFQPMG TVCREAVNDC DIRETCSGNS SQCAPNIHKM DGYSCDGVQG
     ICFGGRCKTR DRQCKYIWGQ KVTASDKYCY EKLNIEGTEK GNCGKDKDTW IQCNKRDVLC
     GYLLCTNIGN IPRLGELDGE ITSTLVVQQG RTLNCSGGHV KLEEDVDLGY VEDGTPCGPQ
     MMCLEHRCLP VASFNFSTCL SSKEGTICSG NGVCSNELKC VCNRHWIGSD CNTYFPHNDD
     AKTGITLSGN GVAGTNIIIG IIAGTILVLA LILGITAWGY KNYREQRQLP QGDYVKKPGD
     GDSFYSDIPP GVSTNSASSS KKRSNGLSHS WSERIPDTKH ISDICENGRP RSNSWQGNLG
     GNKKKIRGKR FRPRSNSTET LSPAKSPSSS TGSIASSRKY PYPMPPLPDE DKKVNRQSAR
     LWETSI
//