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Protein

E3 ubiquitin-protein ligase KCMF1

Gene

KCMF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5048ZZ-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri78 – 10124C2H2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase KCMF1 (EC:6.3.2.-)
Alternative name(s):
FGF-induced in gastric cancer
Potassium channel modulatory factor
Short name:
PCMF
ZZ-type zinc finger-containing protein 1
Gene namesi
Name:KCMF1
Synonyms:FIGC, ZZZ1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:20589. KCMF1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134967694.

Polymorphism and mutation databases

BioMutaiKCMF1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 381380E3 ubiquitin-protein ligase KCMF1PRO_0000349219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei212 – 2121PhosphoserineCombined sources
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei336 – 3361PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9P0J7.
MaxQBiQ9P0J7.
PaxDbiQ9P0J7.
PRIDEiQ9P0J7.

PTM databases

iPTMnetiQ9P0J7.

Expressioni

Tissue specificityi

Spleen, small intestine, ovary, peripheral blood, lung, kidney and pancreas. Expressed at low levels in the thymus, prostate, testis, colon, heart, brain, placenta and liver.1 Publication

Inductioni

Up-regulated by FGF2 in gastric cancer cells.1 Publication

Gene expression databases

BgeeiQ9P0J7.
CleanExiHS_KCMF1.
ExpressionAtlasiQ9P0J7. baseline and differential.
GenevisibleiQ9P0J7. HS.

Organism-specific databases

HPAiHPA030383.
HPA030384.

Interactioni

Protein-protein interaction databases

BioGridi121218. 63 interactions.
DIPiDIP-47300N.
IntActiQ9P0J7. 5 interactions.
STRINGi9606.ENSP00000386738.

Structurei

3D structure databases

ProteinModelPortaliQ9P0J7.
SMRiQ9P0J7. Positions 2-59.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili225 – 25733Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi333 – 3364Poly-Ser
Compositional biasi376 – 3805Poly-Pro

Sequence similaritiesi

Belongs to the KCMF1 family.Curated
Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation
Contains 1 ZZ-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 5048ZZ-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri78 – 10124C2H2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1280. Eukaryota.
ENOG410XRX5. LUCA.
GeneTreeiENSGT00510000047171.
HOGENOMiHOG000007727.
HOVERGENiHBG101229.
InParanoidiQ9P0J7.
OMAiIGVSCDA.
PhylomeDBiQ9P0J7.
TreeFamiTF318128.

Family and domain databases

InterProiIPR008598. Di19_Zn_binding_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
PROSITEiPS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P0J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRHEGVSCD ACLKGNFRGR RYKCLICYDY DLCASCYESG ATTTRHTTDH
60 70 80 90 100
PMQCILTRVD FDLYYGGEAF SVEQPQSFTC PYCGKMGYTE TSLQEHVTSE
110 120 130 140 150
HAETSTEVIC PICAALPGGD PNHVTDDFAA HLTLEHRAPR DLDESSGVRH
160 170 180 190 200
VRRMFHPGRG LGGPRARRSN MHFTSSSTGG LSSSQSSYSP SNREAMDPIA
210 220 230 240 250
ELLSQLSGVR RSAGGQLNSS GPSASQLQQL QMQLQLERQH AQAARQQLET
260 270 280 290 300
ARNATRRTNT SSVTTTITQS TATTNIANTE SSQQTLQNSQ FLLTRLNDPK
310 320 330 340 350
MSETERQSME SERADRSLFV QELLLSTLVR EESSSSDEDD RGEMADFGAM
360 370 380
GCVDIMPLDV ALENLNLKES NKGNEPPPPP L
Length:381
Mass (Da):41,945
Last modified:September 2, 2008 - v2
Checksum:i23B2279C56CF199B
GO

Sequence cautioni

The sequence AAX88897.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti95 – 951E → G in BAB14563 (PubMed:14702039).Curated
Sequence conflicti242 – 2421Q → R in BAC43745 (PubMed:15581609).Curated
Sequence conflicti242 – 2421Q → R in AAF67009 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083199 mRNA. Translation: BAC43745.1.
AF155652 mRNA. Translation: AAF67009.1.
AK023403 mRNA. Translation: BAB14563.1.
AK314761 mRNA. Translation: BAG37299.1.
AC022210 Genomic DNA. Translation: AAY24192.1.
AC078974 Genomic DNA. Translation: AAX88897.1. Different initiation.
BC000178 mRNA. Translation: AAH00178.1.
AL122115 mRNA. Translation: CAB59274.1.
CCDSiCCDS46350.1.
PIRiT34540.
RefSeqiNP_064507.3. NM_020122.4.
UniGeneiHs.654968.

Genome annotation databases

EnsembliENST00000409785; ENSP00000386738; ENSG00000176407.
GeneIDi56888.
KEGGihsa:56888.
UCSCiuc002sox.5. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB083199 mRNA. Translation: BAC43745.1.
AF155652 mRNA. Translation: AAF67009.1.
AK023403 mRNA. Translation: BAB14563.1.
AK314761 mRNA. Translation: BAG37299.1.
AC022210 Genomic DNA. Translation: AAY24192.1.
AC078974 Genomic DNA. Translation: AAX88897.1. Different initiation.
BC000178 mRNA. Translation: AAH00178.1.
AL122115 mRNA. Translation: CAB59274.1.
CCDSiCCDS46350.1.
PIRiT34540.
RefSeqiNP_064507.3. NM_020122.4.
UniGeneiHs.654968.

3D structure databases

ProteinModelPortaliQ9P0J7.
SMRiQ9P0J7. Positions 2-59.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121218. 63 interactions.
DIPiDIP-47300N.
IntActiQ9P0J7. 5 interactions.
STRINGi9606.ENSP00000386738.

PTM databases

iPTMnetiQ9P0J7.

Polymorphism and mutation databases

BioMutaiKCMF1.

Proteomic databases

EPDiQ9P0J7.
MaxQBiQ9P0J7.
PaxDbiQ9P0J7.
PRIDEiQ9P0J7.

Protocols and materials databases

DNASUi56888.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409785; ENSP00000386738; ENSG00000176407.
GeneIDi56888.
KEGGihsa:56888.
UCSCiuc002sox.5. human.

Organism-specific databases

CTDi56888.
GeneCardsiKCMF1.
H-InvDBHIX0020753.
HGNCiHGNC:20589. KCMF1.
HPAiHPA030383.
HPA030384.
MIMi614719. gene.
neXtProtiNX_Q9P0J7.
PharmGKBiPA134967694.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1280. Eukaryota.
ENOG410XRX5. LUCA.
GeneTreeiENSGT00510000047171.
HOGENOMiHOG000007727.
HOVERGENiHBG101229.
InParanoidiQ9P0J7.
OMAiIGVSCDA.
PhylomeDBiQ9P0J7.
TreeFamiTF318128.

Miscellaneous databases

ChiTaRSiKCMF1. human.
GenomeRNAii56888.
PROiQ9P0J7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9P0J7.
CleanExiHS_KCMF1.
ExpressionAtlasiQ9P0J7. baseline and differential.
GenevisibleiQ9P0J7. HS.

Family and domain databases

InterProiIPR008598. Di19_Zn_binding_dom.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR000433. Znf_ZZ.
[Graphical view]
PfamiPF05605. zf-Di19. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
PROSITEiPS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "FIGC, a novel FGF-induced ubiquitin-protein ligase in gastric cancers."
    Jang J.-H.
    FEBS Lett. 578:21-25(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
    Tissue: Stomach.
  2. "A novel gene expressed in the human adrenal gland."
    Ren S., Li Y., Huang C., Jiang C., Gu W., Zhou J., Yu Y., Xu S., Wang Y., Fu G., Chen Z., Han Z.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Adrenal gland.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary and Testis.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Cervix.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 102-381.
    Tissue: Testis.
  6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335 AND SER-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiKCMF1_HUMAN
AccessioniPrimary (citable) accession number: Q9P0J7
Secondary accession number(s): Q4ZG04
, Q53SC7, Q9BWK2, Q9H8P5, Q9UFE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: June 8, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.