Q9P0J7 (KCMF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase KCMF1
EC=6.3.2.-

Alternative name(s):
FGF-induced in gastric cancer
Potassium channel modulatory factor
Short name=PCMF
ZZ-type zinc finger-containing protein 1

Gene names

Name:	KCMF1
Synonyms:	FIGC, ZZZ1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has intrinsic E3 ubiquitin ligase activity and promotes ubiquitination. Ref.1
Tissue specificity	Spleen, small intestine, ovary, peripheral blood, lung, kidney and pancreas. Expressed at low levels in the thymus, prostate, testis, colon, heart, brain, placenta and liver. Ref.1
Induction	Up-regulated by FGF2 in gastric cancer cells. Ref.1
Sequence similarities	Belongs to the KCMF1 family. Contains 1 C2H2-type zinc finger. Contains 1 ZZ-type zinc finger.
Sequence caution	The sequence AAX88897.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	E3 ubiquitin-protein ligase KCMF1		PRO_0000349219
Regions
	Zinc finger	3 – 50	48	ZZ-type
	Zinc finger	78 – 101	24	C2H2-type
	Coiled coil	225 – 257	33	Potential
	Compositional bias	333 – 336	4	Poly-Ser
	Compositional bias	376 – 380	5	Poly-Pro
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	95	1	E → G in BAB14563. Ref.3
	Sequence conflict	242	1	Q → R in BAC43745. Ref.1
	Sequence conflict	242	1	Q → R in AAF67009. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9P0J7 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 23B2279C56CF199B	FASTA	381	41,945
	10 20 30 40 50 60 MSRHEGVSCD ACLKGNFRGR RYKCLICYDY DLCASCYESG ATTTRHTTDH PMQCILTRVD 70 80 90 100 110 120 FDLYYGGEAF SVEQPQSFTC PYCGKMGYTE TSLQEHVTSE HAETSTEVIC PICAALPGGD 130 140 150 160 170 180 PNHVTDDFAA HLTLEHRAPR DLDESSGVRH VRRMFHPGRG LGGPRARRSN MHFTSSSTGG 190 200 210 220 230 240 LSSSQSSYSP SNREAMDPIA ELLSQLSGVR RSAGGQLNSS GPSASQLQQL QMQLQLERQH 250 260 270 280 290 300 AQAARQQLET ARNATRRTNT SSVTTTITQS TATTNIANTE SSQQTLQNSQ FLLTRLNDPK 310 320 330 340 350 360 MSETERQSME SERADRSLFV QELLLSTLVR EESSSSDEDD RGEMADFGAM GCVDIMPLDV 370 380 ALENLNLKES NKGNEPPPPP L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"FIGC, a novel FGF-induced ubiquitin-protein ligase in gastric cancers." Jang J.-H. FEBS Lett. 578:21-25(2004) [PubMed: 15581609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY. Tissue: Stomach.
[2]	"A novel gene expressed in the human adrenal gland." Ren S., Li Y., Huang C., Jiang C., Gu W., Zhou J., Yu Y., Xu S., Wang Y., Fu G., Chen Z., Han Z. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Adrenal gland.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary and Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 102-381. Tissue: Testis.
[6]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083199 mRNA. Translation: BAC43745.1. AF155652 mRNA. Translation: AAF67009.1. AK023403 mRNA. Translation: BAB14563.1. AK314761 mRNA. Translation: BAG37299.1. AC022210 Genomic DNA. Translation: AAY24192.1. AC078974 Genomic DNA. Translation: AAX88897.1. Different initiation. BC000178 mRNA. Translation: AAH00178.1. AL122115 mRNA. Translation: CAB59274.1.
IPI	IPI00306661.
PIR	T34540.
RefSeq	NP_064507.3. NM_020122.4.
UniGene	Hs.654968.
3D structure databases
ProteinModelPortal	Q9P0J7.
SMR	Q9P0J7. Positions 6-55.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47300N.
STRING	Q9P0J7.
Polymorphism databases
DMDM	205815553.
Proteomic databases
PRIDE	Q9P0J7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000409785; ENSP00000386738; ENSG00000176407.
GeneID	56888.
KEGG	hsa:56888.
Organism-specific databases
CTD	56888.
GeneCards	GC02P085198.
H-InvDB	HIX0020753.
HGNC	HGNC:20589. KCMF1.
HPA	HPA030383. HPA030384.
neXtProt	NX_Q9P0J7.
PharmGKB	PA134967694.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13025.
GeneTree	ENSGT00510000047171.
HOVERGEN	HBG101229.
Gene expression databases
ArrayExpress	Q9P0J7.
Bgee	Q9P0J7.
CleanEx	HS_KCMF1.
Genevestigator	Q9P0J7.
Family and domain databases
InterPro	IPR008598. Di19_RING_finger_144. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR000433. Znf_ZZ. [Graphical view]
Pfam	PF05605. Di19. 1 hit. PF00569. ZZ. 1 hit. [Graphical view]
SMART	SM00355. ZnF_C2H2. 1 hit. SM00291. ZnF_ZZ. 1 hit. [Graphical view]
PROSITE	PS01357. ZF_ZZ_1. 1 hit. PS50135. ZF_ZZ_2. 1 hit. PS00028. ZINC_FINGER_C2H2_1. False negative. PS50157. ZINC_FINGER_C2H2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCMF1_HUMAN

Accession

Primary (citable) accession number: Q9P0J7
Secondary accession number(s): Q4ZG04 , Q53SC7, Q9BWK2, Q9H8P5, Q9UFE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.