##gff-version 3
Q9P0I2	UniProtKB	Initiator methionine	1	1	.	.	.	Note=Removed;Ontology_term=ECO:0000269;evidence=ECO:0000269|Ref.6	
Q9P0I2	UniProtKB	Chain	2	261	.	.	.	ID=PRO_0000211406;Note=ER membrane protein complex subunit 3	
Q9P0I2	UniProtKB	Topological domain	2	14	.	.	.	Note=Lumenal;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Transmembrane	15	38	.	.	.	Note=Helical;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Topological domain	39	114	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Transmembrane	115	130	.	.	.	Note=Helical;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Topological domain	131	168	.	.	.	Note=Lumenal;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Transmembrane	169	187	.	.	.	Note=Helical;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Topological domain	188	261	.	.	.	Note=Cytoplasmic;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Alternative sequence	220	261	.	.	.	ID=VSP_014886;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|Ref.3	
Q9P0I2	UniProtKB	Mutagenesis	31	31	.	.	.	Note=No effect on EMC assembly but decreased membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC. R->A%2CE;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	31	31	.	.	.	Note=No effect on EMC assembly and no effect on membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC. R->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	101	101	.	.	.	Note=No effect on EMC assembly but decreased membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC%3B when associated S-106%2C S-110 and S-111. M->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	106	106	.	.	.	Note=No effect on EMC assembly but decreased membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC%3B when associated S-101%2C S-110 and S-111. M->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	110	110	.	.	.	Note=No effect on EMC assembly but decreased membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC%3B when associated S-101%2C S-106 and S-111. M->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	111	111	.	.	.	Note=No effect on EMC assembly but decreased membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC%3B when associated S-101%2C S-106 and S-110. M->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	173	173	.	.	.	Note=No effect on EMC assembly and no effect on membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC. F->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	180	180	.	.	.	Note=No effect on EMC assembly but decreased membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC. R->A%2CE;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Mutagenesis	180	180	.	.	.	Note=No effect on EMC assembly and no effect on membrane insertion of hydrophobic transmembrane helices-containing proteins by the EMC. R->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:32439656;Dbxref=PMID:32439656	
Q9P0I2	UniProtKB	Sequence conflict	29	29	.	.	.	Note=M->T;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9P0I2	UniProtKB	Sequence conflict	60	60	.	.	.	Note=V->I;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9P0I2	UniProtKB	Sequence conflict	172	172	.	.	.	Note=Y->H;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9P0I2	UniProtKB	Sequence conflict	250	250	.	.	.	Note=G->D;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q9P0I2	UniProtKB	Helix	10	16	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	18	38	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	46	64	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	65	67	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:8EOI	
Q9P0I2	UniProtKB	Helix	70	81	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Turn	83	85	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	87	89	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	115	131	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	146	148	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WW7	
Q9P0I2	UniProtKB	Helix	149	151	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Beta strand	153	155	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Beta strand	159	161	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WW7	
Q9P0I2	UniProtKB	Beta strand	163	166	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6WW7	
Q9P0I2	UniProtKB	Helix	168	178	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	180	182	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	184	186	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Beta strand	187	190	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:8EOI	
Q9P0I2	UniProtKB	Helix	214	226	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO	
Q9P0I2	UniProtKB	Helix	237	241	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7ADO