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Q9P0I2 (EMC3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ER membrane protein complex subunit 3
Alternative name(s):
Transmembrane protein 111
Gene names
Name:EMC3
Synonyms:TMEM111
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the ER membrane protein complex (EMC). Ref.8

Subcellular location

Membrane; Multi-pass membrane protein Ref.8.

Sequence similarities

Belongs to the EMC3 family.

Sequence caution

The sequence BAD18807.1 differs from that shown. Reason: Erroneous termination at position 222. Translated as Trp.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentER membrane protein complex

Inferred from direct assay Ref.8. Source: UniProtKB

integral component of membrane

Inferred from direct assay Ref.8. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9P0I2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9P0I2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     220-261: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 261260ER membrane protein complex subunit 3
PRO_0000211406

Regions

Transmembrane14 – 3421Helical; Potential
Transmembrane118 – 13821Helical; Potential

Natural variations

Alternative sequence220 – 26142Missing in isoform 2.
VSP_014886

Experimental info

Sequence conflict291M → T in BAD18807. Ref.2
Sequence conflict601V → I in BAD96673. Ref.3
Sequence conflict1721Y → H in BAD18807. Ref.2
Sequence conflict2501G → D in BAD18807. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 0FB279E54D6C1FCC

FASTA26129,952
        10         20         30         40         50         60 
MAGPELLLDS NIRLWVVLPI VIITFFVGMI RHYVSILLQS DKKLTQEQVS DSQVLIRSRV 

        70         80         90        100        110        120 
LRENGKYIPK QSFLTRKYYF NNPEDGFFKK TKRKVVPPSP MTDPTMLTDM MKGNVTNVLP 

       130        140        150        160        170        180 
MILIGGWINM TFSGFVTTKV PFPLTLRFKP MLQQGIELLT LDASWVSSAS WYFLNVFGLR 

       190        200        210        220        230        240 
SIYSLILGQD NAADQSRMMQ EQMTGAAMAM PADTNKAFKT EWEALELTDH QWALDDVEEE 

       250        260 
LMAKDLHFEG MFKKELQTSI F 

« Hide

Isoform 2 [UniParc].

Checksum: 2591071C7822CBBC
Show »

FASTA21924,915

References

« Hide 'large scale' references
[1]"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. expand/collapse author list , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Adrenal gland.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Stomach cancer.
[3]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Small intestine.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]Bienvenut W.V.
Submitted (JUN-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-13; 140-147 AND 181-197, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Defining human ERAD networks through an integrative mapping strategy."
Christianson J.C., Olzmann J.A., Shaler T.A., Sowa M.E., Bennett E.J., Richter C.M., Tyler R.E., Greenblatt E.J., Harper J.W., Kopito R.R.
Nat. Cell Biol. 14:93-105(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE EMC COMPLEX, SUBCELLULAR LOCATION.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF157321 mRNA. Translation: AAF67487.1.
AK172843 mRNA. Translation: BAD18807.1. Sequence problems.
AK222953 mRNA. Translation: BAD96673.1.
AK312008 mRNA. Translation: BAG34946.1.
CH471055 Genomic DNA. Translation: EAW64047.1.
BC022807 mRNA. Translation: AAH22807.1.
CCDSCCDS2594.1. [Q9P0I2-1]
RefSeqNP_060917.1. NM_018447.2. [Q9P0I2-1]
UniGeneHs.475392.

3D structure databases

ProteinModelPortalQ9P0I2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120936. 2 interactions.
STRING9606.ENSP00000245046.

PTM databases

PhosphoSiteQ9P0I2.

Polymorphism databases

DMDM71153383.

Proteomic databases

MaxQBQ9P0I2.
PaxDbQ9P0I2.
PRIDEQ9P0I2.

Protocols and materials databases

DNASU55831.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000245046; ENSP00000245046; ENSG00000125037. [Q9P0I2-1]
GeneID55831.
KEGGhsa:55831.
UCSCuc003bun.3. human. [Q9P0I2-1]

Organism-specific databases

CTD55831.
GeneCardsGC03M010007.
HGNCHGNC:23999. EMC3.
HPAHPA042372.
neXtProtNX_Q9P0I2.
PharmGKBPA142670762.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG306838.
HOGENOMHOG000158234.
HOVERGENHBG056099.
InParanoidQ9P0I2.
OMANIRGWVF.
OrthoDBEOG708W0H.
PhylomeDBQ9P0I2.

Gene expression databases

ArrayExpressQ9P0I2.
BgeeQ9P0I2.
CleanExHS_TMEM111.
GenevestigatorQ9P0I2.

Family and domain databases

InterProIPR002809. DUF106_TM.
IPR008568. UCP010045_TM_euk.
[Graphical view]
PANTHERPTHR13116. PTHR13116. 1 hit.
PfamPF01956. DUF106. 1 hit.
[Graphical view]
PIRSFPIRSF010045. DUF850_TM_euk. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi55831.
NextBio61045.
PROQ9P0I2.

Entry information

Entry nameEMC3_HUMAN
AccessionPrimary (citable) accession number: Q9P0I2
Secondary accession number(s): B2R4Z9, Q53GH8, Q6ZMC2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM