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Protein

ER membrane protein complex subunit 3

Gene

EMC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
ER membrane protein complex subunit 3
Alternative name(s):
Transmembrane protein 111
Gene namesi
Name:EMC3
Synonyms:TMEM111
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:23999. EMC3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • ER membrane protein complex Source: UniProtKB
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670762.

Polymorphism and mutation databases

BioMutaiEMC3.
DMDMi71153383.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 261260ER membrane protein complex subunit 3PRO_0000211406Add
BLAST

Proteomic databases

MaxQBiQ9P0I2.
PaxDbiQ9P0I2.
PRIDEiQ9P0I2.

PTM databases

PhosphoSiteiQ9P0I2.

Expressioni

Gene expression databases

BgeeiQ9P0I2.
CleanExiHS_TMEM111.
ExpressionAtlasiQ9P0I2. baseline and differential.
GenevisibleiQ9P0I2. HS.

Organism-specific databases

HPAiHPA042372.

Interactioni

Subunit structurei

Component of the ER membrane protein complex (EMC).1 Publication

Protein-protein interaction databases

BioGridi120936. 18 interactions.
STRINGi9606.ENSP00000245046.

Structurei

3D structure databases

ProteinModelPortaliQ9P0I2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EMC3 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG306838.
GeneTreeiENSGT00390000005780.
HOGENOMiHOG000158234.
HOVERGENiHBG056099.
InParanoidiQ9P0I2.
OMAiNIRGWVF.
OrthoDBiEOG708W0H.
PhylomeDBiQ9P0I2.

Family and domain databases

InterProiIPR002809. DUF106_TM.
IPR008568. UCP010045_TM_euk.
[Graphical view]
PANTHERiPTHR13116. PTHR13116. 1 hit.
PfamiPF01956. DUF106. 1 hit.
[Graphical view]
PIRSFiPIRSF010045. DUF850_TM_euk. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9P0I2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPELLLDS NIRLWVVLPI VIITFFVGMI RHYVSILLQS DKKLTQEQVS
60 70 80 90 100
DSQVLIRSRV LRENGKYIPK QSFLTRKYYF NNPEDGFFKK TKRKVVPPSP
110 120 130 140 150
MTDPTMLTDM MKGNVTNVLP MILIGGWINM TFSGFVTTKV PFPLTLRFKP
160 170 180 190 200
MLQQGIELLT LDASWVSSAS WYFLNVFGLR SIYSLILGQD NAADQSRMMQ
210 220 230 240 250
EQMTGAAMAM PADTNKAFKT EWEALELTDH QWALDDVEEE LMAKDLHFEG
260
MFKKELQTSI F
Length:261
Mass (Da):29,952
Last modified:January 23, 2007 - v3
Checksum:i0FB279E54D6C1FCC
GO
Isoform 2 (identifier: Q9P0I2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-261: Missing.

Note: No experimental confirmation available.
Show »
Length:219
Mass (Da):24,915
Checksum:i2591071C7822CBBC
GO

Sequence cautioni

The sequence BAD18807.1 differs from that shown. Reason: Erroneous termination at position 222. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291M → T in BAD18807 (PubMed:14702039).Curated
Sequence conflicti60 – 601V → I in BAD96673 (Ref. 3) Curated
Sequence conflicti172 – 1721Y → H in BAD18807 (PubMed:14702039).Curated
Sequence conflicti250 – 2501G → D in BAD18807 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei220 – 26142Missing in isoform 2. 1 PublicationVSP_014886Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157321 mRNA. Translation: AAF67487.1.
AK172843 mRNA. Translation: BAD18807.1. Sequence problems.
AK222953 mRNA. Translation: BAD96673.1.
AK312008 mRNA. Translation: BAG34946.1.
CH471055 Genomic DNA. Translation: EAW64047.1.
BC022807 mRNA. Translation: AAH22807.1.
CCDSiCCDS2594.1. [Q9P0I2-1]
RefSeqiNP_060917.1. NM_018447.2. [Q9P0I2-1]
XP_011532258.1. XM_011533956.1. [Q9P0I2-1]
UniGeneiHs.475392.

Genome annotation databases

EnsembliENST00000245046; ENSP00000245046; ENSG00000125037.
GeneIDi55831.
KEGGihsa:55831.
UCSCiuc003bun.3. human. [Q9P0I2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157321 mRNA. Translation: AAF67487.1.
AK172843 mRNA. Translation: BAD18807.1. Sequence problems.
AK222953 mRNA. Translation: BAD96673.1.
AK312008 mRNA. Translation: BAG34946.1.
CH471055 Genomic DNA. Translation: EAW64047.1.
BC022807 mRNA. Translation: AAH22807.1.
CCDSiCCDS2594.1. [Q9P0I2-1]
RefSeqiNP_060917.1. NM_018447.2. [Q9P0I2-1]
XP_011532258.1. XM_011533956.1. [Q9P0I2-1]
UniGeneiHs.475392.

3D structure databases

ProteinModelPortaliQ9P0I2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120936. 18 interactions.
STRINGi9606.ENSP00000245046.

PTM databases

PhosphoSiteiQ9P0I2.

Polymorphism and mutation databases

BioMutaiEMC3.
DMDMi71153383.

Proteomic databases

MaxQBiQ9P0I2.
PaxDbiQ9P0I2.
PRIDEiQ9P0I2.

Protocols and materials databases

DNASUi55831.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000245046; ENSP00000245046; ENSG00000125037.
GeneIDi55831.
KEGGihsa:55831.
UCSCiuc003bun.3. human. [Q9P0I2-1]

Organism-specific databases

CTDi55831.
GeneCardsiGC03M010007.
HGNCiHGNC:23999. EMC3.
HPAiHPA042372.
neXtProtiNX_Q9P0I2.
PharmGKBiPA142670762.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG306838.
GeneTreeiENSGT00390000005780.
HOGENOMiHOG000158234.
HOVERGENiHBG056099.
InParanoidiQ9P0I2.
OMAiNIRGWVF.
OrthoDBiEOG708W0H.
PhylomeDBiQ9P0I2.

Miscellaneous databases

GenomeRNAii55831.
NextBioi61045.
PROiQ9P0I2.

Gene expression databases

BgeeiQ9P0I2.
CleanExiHS_TMEM111.
ExpressionAtlasiQ9P0I2. baseline and differential.
GenevisibleiQ9P0I2. HS.

Family and domain databases

InterProiIPR002809. DUF106_TM.
IPR008568. UCP010045_TM_euk.
[Graphical view]
PANTHERiPTHR13116. PTHR13116. 1 hit.
PfamiPF01956. DUF106. 1 hit.
[Graphical view]
PIRSFiPIRSF010045. DUF850_TM_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Adrenal gland.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain and Stomach cancer.
  3. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Small intestine.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  6. Bienvenut W.V.
    Submitted (JUN-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-13; 140-147 AND 181-197, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: B-cell lymphoma.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION IN THE EMC COMPLEX, SUBCELLULAR LOCATION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiEMC3_HUMAN
AccessioniPrimary (citable) accession number: Q9P0I2
Secondary accession number(s): B2R4Z9, Q53GH8, Q6ZMC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.