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Q9P0G3

- KLK14_HUMAN

UniProt

Q9P0G3 - KLK14_HUMAN

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Protein

Kallikrein-14

Gene

KLK14

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis.6 Publications

Enzyme regulationi

Inhibited by SERPINA1, SERPINC1, SERPINE1, SERPINF2, aprotinin, soybean, trypsin inhibitor and leupeptin. Inhibited by serine protease inhibitor SPINK5. Has an autoproteolytic activity which may have a regulatory effect. Activated by citrate and inhibited by zinc and to a lower extent by manganese.3 Publications

Kineticsi

Has a higher catalytic efficiency for the trypsin-like enzyme substrates S-2288, S-2222 and S-2302 compared to S-2586 a chymotrypsin-like enzyme substrate. Has a lower catalytic activity compared to trypsin towards S-2288, S-2222 and S-2302. Cleaves preferentially after Arg residues.

  1. KM=0.3 mM for S-22882 Publications
  2. KM=0.2 mM for S-22222 Publications
  3. KM=0.2 mM for S-23022 Publications
  4. KM=0.7 mM for S-25862 Publications
  5. KM=0.045 mM for Gln-Ala-Arg synthetic peptide2 Publications
  6. KM=0.043 mM for Val-Pro-Arg synthetic peptide2 Publications
  7. KM=0.09 mM for Pro-Phe-Arg synthetic peptide2 Publications
  8. KM=0.278 mM for Phe-Ser-Arg synthetic peptide2 Publications
  9. KM=0.0577 mM for Leu-Gly-Arg synthetic peptide2 Publications
  10. KM=0.139 mM for Gln-Gly-Arg synthetic peptide2 Publications
  11. KM=0.173 mM for Gly-Pro-Arg synthetic peptide2 Publications
  12. KM=0.0268 mM for Gln-Arg-Arg synthetic peptide2 Publications
  13. KM=0.130 mM for Gly-Gly-Arg synthetic peptide2 Publications
  14. KM=0.578 mM for Val-Leu-Lys synthetic peptide2 Publications

pH dependencei

Optimum pH is 8.0.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei83 – 831Charge relay systemBy similarity
Active sitei127 – 1271Charge relay systemBy similarity
Active sitei220 – 2201Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  1. epidermis morphogenesis Source: UniProtKB
  2. fertilization Source: UniProtKB
  3. negative regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  4. positive regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  5. proteolysis Source: UniProtKB
  6. seminal clot liquefaction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.029.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-14 (EC:3.4.21.-)
Short name:
hK14
Alternative name(s):
Kallikrein-like protein 6
Short name:
KLK-L6
Gene namesi
Name:KLK14
Synonyms:KLKL6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:6362. KLK14.

Subcellular locationi

Secretedextracellular space 3 Publications

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
  2. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30151.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Add
BLAST
Propeptidei35 – 406Activation peptide1 PublicationPRO_0000027958
Chaini41 – 267227Kallikrein-14PRO_0000027959Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 180PROSITE-ProRule annotation
Disulfide bondi68 ↔ 84PROSITE-ProRule annotation
Disulfide bondi159 ↔ 226PROSITE-ProRule annotation
Disulfide bondi191 ↔ 205PROSITE-ProRule annotation
Disulfide bondi216 ↔ 241PROSITE-ProRule annotation

Post-translational modificationi

Proteolytic cleavage of the activation peptide produces the active enzyme.1 Publication

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Zymogen

Proteomic databases

PaxDbiQ9P0G3.
PRIDEiQ9P0G3.

PTM databases

PhosphoSiteiQ9P0G3.

Miscellaneous databases

PMAP-CutDBQ9P0G3.

Expressioni

Tissue specificityi

Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level).6 Publications

Inductioni

Up-regulated by steroid hormone.1 Publication

Gene expression databases

BgeeiQ9P0G3.
CleanExiHS_KLK14.
GenevestigatoriQ9P0G3.

Organism-specific databases

HPAiCAB026228.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000156499.

Structurei

3D structure databases

ProteinModelPortaliQ9P0G3.
SMRiQ9P0G3. Positions 41-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 265225Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9P0G3.
KOiK09622.
OMAiGMERCAQ.
OrthoDBiEOG7MKW6Q.
PhylomeDBiQ9P0G3.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P0G3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLRVLGSGT WPSAPKMFLL LTALQVLAIA MTQSQEDENK IIGGHTCTRS
60 70 80 90 100
SQPWQAALLA GPRRRFLCGG ALLSGQWVIT AAHCGRPILQ VALGKHNLRR
110 120 130 140 150
WEATQQVLRV VRQVTHPNYN SRTHDNDLML LQLQQPARIG RAVRPIEVTQ
160 170 180 190 200
ACASPGTSCR VSGWGTISSP IARYPASLQC VNINISPDEV CQKAYPRTIT
210 220 230 240 250
PGMVCAGVPQ GGKDSCQGDS GGPLVCRGQL QGLVSWGMER CALPGYPGVY
260
TNLCKYRSWI EETMRDK
Length:267
Mass (Da):29,122
Last modified:July 7, 2009 - v2
Checksum:i0CE085DA7BD1D92B
GO

Sequence cautioni

The sequence AAK48524.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAD50773.2 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAG23260.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAK48523.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence ABU63131.1 differs from that shown. Reason: Erroneous gene model prediction.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331Q → R.1 Publication
Corresponds to variant rs35287116 [ dbSNP | Ensembl ].
VAR_058018
Natural varianti45 – 451H → Y.1 Publication
Corresponds to variant rs2569491 [ dbSNP | Ensembl ].
VAR_058019
Natural varianti64 – 641R → H.
Corresponds to variant rs2569490 [ dbSNP | Ensembl ].
VAR_058020

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF161221 Genomic DNA. Translation: AAD50773.2. Sequence problems.
AF283669 Genomic DNA. Translation: AAK48523.1. Sequence problems.
AF283670 mRNA. Translation: AAK48524.1. Different initiation.
EU091477 Genomic DNA. Translation: ABU63131.1. Sequence problems.
AC011473 Genomic DNA. Translation: AAG23260.1. Sequence problems.
CH471135 Genomic DNA. Translation: EAW71982.1.
BC074904 mRNA. Translation: AAH74904.2.
BC074905 mRNA. Translation: AAH74905.2.
BC114614 mRNA. Translation: AAI14615.2.
CCDSiCCDS12823.2.
RefSeqiNP_071329.2. NM_022046.4.
XP_006723288.1. XM_006723225.1.
UniGeneiHs.283925.

Genome annotation databases

EnsembliENST00000156499; ENSP00000156499; ENSG00000129437.
ENST00000391802; ENSP00000375678; ENSG00000129437.
GeneIDi43847.
KEGGihsa:43847.
UCSCiuc002pvs.1. human.

Polymorphism databases

DMDMi251757292.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF161221 Genomic DNA. Translation: AAD50773.2 . Sequence problems.
AF283669 Genomic DNA. Translation: AAK48523.1 . Sequence problems.
AF283670 mRNA. Translation: AAK48524.1 . Different initiation.
EU091477 Genomic DNA. Translation: ABU63131.1 . Sequence problems.
AC011473 Genomic DNA. Translation: AAG23260.1 . Sequence problems.
CH471135 Genomic DNA. Translation: EAW71982.1 .
BC074904 mRNA. Translation: AAH74904.2 .
BC074905 mRNA. Translation: AAH74905.2 .
BC114614 mRNA. Translation: AAI14615.2 .
CCDSi CCDS12823.2.
RefSeqi NP_071329.2. NM_022046.4.
XP_006723288.1. XM_006723225.1.
UniGenei Hs.283925.

3D structure databases

ProteinModelPortali Q9P0G3.
SMRi Q9P0G3. Positions 41-265.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000156499.

Chemistry

BindingDBi Q9P0G3.
ChEMBLi CHEMBL2641.

Protein family/group databases

MEROPSi S01.029.

PTM databases

PhosphoSitei Q9P0G3.

Polymorphism databases

DMDMi 251757292.

Proteomic databases

PaxDbi Q9P0G3.
PRIDEi Q9P0G3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000156499 ; ENSP00000156499 ; ENSG00000129437 .
ENST00000391802 ; ENSP00000375678 ; ENSG00000129437 .
GeneIDi 43847.
KEGGi hsa:43847.
UCSCi uc002pvs.1. human.

Organism-specific databases

CTDi 43847.
GeneCardsi GC19M051580.
HGNCi HGNC:6362. KLK14.
HPAi CAB026228.
MIMi 606135. gene.
neXtProti NX_Q9P0G3.
PharmGKBi PA30151.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00760000118862.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi Q9P0G3.
KOi K09622.
OMAi GMERCAQ.
OrthoDBi EOG7MKW6Q.
PhylomeDBi Q9P0G3.
TreeFami TF331065.

Miscellaneous databases

GeneWikii KLK14.
GenomeRNAii 43847.
NextBioi 53000.
PMAP-CutDB Q9P0G3.
PROi Q9P0G3.
SOURCEi Search...

Gene expression databases

Bgeei Q9P0G3.
CleanExi HS_KLK14.
Genevestigatori Q9P0G3.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a new member of the human kallikrein gene family, KLK14, which is down-regulated in different malignancies."
    Yousef G.M., Magklara A., Chang A., Jung K., Katsaros D., Diamandis E.P.
    Cancer Res. 61:3425-3431(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
  2. "Identification and characterization of KLK14, a novel kallikrein serine protease gene located on human chromosome 19q13.4 and expressed in prostate and skeletal muscle."
    Hooper J.D., Bui L.T., Rae F.K., Harvey T.J., Myers S.A., Ashworth L.K., Clements J.A.
    Genomics 73:117-122(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
  3. "Understanding the accuracy of statistical haplotype inference with sequence data of known phase."
    Andres A.M., Clark A.G., Shimmin L., Boerwinkle E., Sing C.F., Hixson J.E.
    Genet. Epidemiol. 31:659-671(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ARG-33 AND TYR-45.
    Tissue: Lung.
  7. "Tissue-specific expression patterns and fine mapping of the human kallikrein (KLK) locus on proximal 19q13.4."
    Harvey T.J., Hooper J.D., Myers S.A., Stephenson S.A., Ashworth L.K., Clements J.A.
    J. Biol. Chem. 275:37397-37406(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Steroid hormone regulation and prognostic value of the human kallikrein gene 14 in ovarian cancer."
    Yousef G.M., Fracchioli S., Scorilas A., Borgono C.A., Iskander L., Puopolo M., Massobrio M., Diamandis E.P., Katsaros D.
    Am. J. Clin. Pathol. 119:346-355(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY STEROID HORMONE.
  9. Cited for: CHARACTERIZATION.
  10. "A proteolytic cascade of kallikreins in the stratum corneum."
    Brattsand M., Stefansson K., Lundh C., Haasum Y., Egelrud T.
    J. Invest. Dermatol. 124:198-203(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
  11. "Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum."
    Stefansson K., Brattsand M., Ny A., Glas B., Egelrud T.
    Biol. Chem. 387:761-768(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 41-55, TISSUE SPECIFICITY.
  12. Cited for: FUNCTION IN G PROTEIN-COUPLED RECEPTOR SIGNALING.
  13. "Quantification of eight tissue kallikreins in the stratum corneum and sweat."
    Komatsu N., Tsai B., Sidiropoulos M., Saijoh K., Levesque M.A., Takehara K., Diamandis E.P.
    J. Invest. Dermatol. 126:925-929(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  14. "Expression and functional characterization of the cancer-related serine protease, human tissue kallikrein 14."
    Borgono C.A., Michael I.P., Shaw J.L.V., Luo L.-Y., Ghosh M.C., Soosaipillai A., Grass L., Katsaros D., Diamandis E.P.
    J. Biol. Chem. 282:2405-2422(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PROTEOLYTIC PROCESSING, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
  15. "A potential role for multiple tissue kallikrein serine proteases in epidermal desquamation."
    Borgono C.A., Michael I.P., Komatsu N., Jayakumar A., Kapadia R., Clayman G.L., Sotiropoulou G., Diamandis E.P.
    J. Biol. Chem. 282:3640-3652(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  16. "Human kallikrein-related peptidase 14 (KLK14) is a new activator component of the KLK proteolytic cascade. Possible function in seminal plasma and skin."
    Emami N., Diamandis E.P.
    J. Biol. Chem. 283:3031-3041(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "Major role of human KLK14 in seminal clot liquefaction."
    Emami N., Deperthes D., Malm J., Diamandis E.P.
    J. Biol. Chem. 283:19561-19569(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "Activation of proteinase-activated receptor-2 by human kallikrein-related peptidases."
    Stefansson K., Brattsand M., Roosterman D., Kempkes C., Bocheva G., Steinhoff M., Egelrud T.
    J. Invest. Dermatol. 128:18-25(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiKLK14_HUMAN
AccessioniPrimary (citable) accession number: Q9P0G3
Secondary accession number(s): A7UNK5, Q1RMZ2, Q6B089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: July 7, 2009
Last modified: October 29, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-17 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3