Reviewed,
UniProtKB/Swiss-Prot Q9NZZ3 (CHMP5_HUMAN)
Last modified
July 7, 2009.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Charged multivesicular body protein 5 Alternative name(s): Chromatin-modifying protein 5 Vacuolar protein-sorting-associated protein 60 Short name=Vps60 Short name=hVps60 SNF7 domain-containing protein 2 | ||||||
| Gene names |
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| Organism | Homo sapiens (Human) [Complete proteome] | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 219 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. Ref.6 |
| Subunit structure | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with VTA1. Interacts with CHMP2A. Interacts with VTA1; the interaction involves soluble CHMP5. Ref.6 Ref.8 Ref.10 |
| Subcellular location | Cytoplasm › cytosol. Endosome membrane; Peripheral membrane protein Probable. Note: Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body. |
| Sequence similarities | Belongs to the SNF7 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein transport Transport |
| Cellular component | Cytoplasm Endosome Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Coiled coil |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | protein transport Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell endosome membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CAMK2A | Q9UQM7 | 1 | EBI-751303,EBI-1383687 | |
| STAMBP | O95630 | 1 | EBI-751303,EBI-396676 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 219 | 219 | Charged multivesicular body protein 5 | PRO_0000211500 | |||||
Regions | |||||||||
| Region | 121 – 158 | 38 | Interaction with VTA1 | ||||||
| Coiled coil | 26 – 179 | 154 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 219 | 1 | Phosphoserine Ref.11 | ||||||
Natural variations | |||||||||
| Natural variant | 86 | 1 | S → P: dbSNP rs11540558. | VAR_052029 | |||||
Experimental info | |||||||||
| Sequence conflict | 14 | 1 | P → R in AAD27743. Ref.1 | ||||||
| Sequence conflict | 19 | 1 | D → G in AAH16698. Ref.4 | ||||||
| Sequence conflict | 84 – 93 | 10 | QQSFNMEQAN → NSHSTWTGH in AAD27743. Ref.1 | ||||||
| Sequence conflict | 122 | 1 | Q → P in AAD27743. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [2] | "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. Chen Z.Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood. |
| [3] | "DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. Dunham I.Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow, Brain, Kidney and Urinary bladder. |
| [5] | "Human acute promyelocytic leukemia cell line NB4's apoptosis related genes." Yu W.-Q., Sun B.-Z., Chai Y.-B., Zhu F., Liu X.-S., Li Z., Lu F., Yan W., Yang H., Zhao Z.-L. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-219. Tissue: Promyelocytic leukemia. |
| [6] | "Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins." Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D. Proc. Natl. Acad. Sci. U.S.A. 100:12414-12419(2003) [PubMed: 14519844] [Abstract] Cited for: FUNCTION IN HIV-1 BUDDING, INTERACTION WITH CHMP2A. |
| [7] | Erratum Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D. Proc. Natl. Acad. Sci. U.S.A. 100:152845-152845(2003) |
| [8] | "The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells." Ward D.M., Vaughn M.B., Shiflett S.L., White P.L., Pollock A.L., Hill J., Schnegelberger R., Sundquist W.I., Kaplan J. J. Biol. Chem. 280:10548-10555(2005) [PubMed: 15644320] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH VTA1. |
| [9] | "Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis." Morita E., Sandrin V., Chung H.Y., Morham S.G., Gygi S.P., Rodesch C.K., Sundquist W.I. EMBO J. 26:4215-4227(2007) [PubMed: 17853893] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| [10] | "Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradation." Ma Y.M., Boucrot E., Villen J., Affar el B., Gygi S.P., Goettlinger H.G., Kirchhausen T. J. Biol. Chem. 282:9805-9812(2007) [PubMed: 17261583] [Abstract] Cited for: INTERACTION WITH VTA1, MASS SPECTROMETRY. |
| [11] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, MASS SPECTROMETRY. |
| [12] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| AF132968 mRNA. Translation: AAD27743.1. AF161525 mRNA. Translation: AAF29140.1. AL356472 Genomic DNA. Translation: CAH72744.1. BC006974 mRNA. Translation: AAH06974.1. BC007457 mRNA. Translation: AAH07457.1. BC016698 mRNA. Translation: AAH16698.1. BC020796 mRNA. Translation: AAH20796.1. BC021168 mRNA. Translation: AAH21168.1. AF275810 mRNA. Translation: AAG23821.1. Different initiation. AF229832 mRNA. Translation: AAF42917.1. | |
| IPI | IPI00100796. |
| RefSeq | NP_057494.3. |
| UniGene | Hs.635313 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9NZZ3. 3 interactions. |
PTM databases | |
| PhosphoSite | Q9NZZ3. |
Proteomic databases | |
| PeptideAtlas | Q9NZZ3. |
| PRIDE | Q9NZZ3. |
Genome annotation databases | |
| Ensembl | ENSG00000086065. Homo sapiens. [Contig view] |
| GeneID | 51510. |
| KEGG | hsa:51510. |
| UCSC | uc003zsl.2. human. |
Organism-specific databases | |
| GeneCards | GC09P033257. |
| H-InvDB | HIX0007979. |
| HGNC | HGNC:26942. CHMP5. |
| MIM | 610900. gene. |
| PharmGKB | PA134903143. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q9NZZ3. |
| HOVERGEN | Q9NZZ3. |
| OMA | Q9NZZ3. QADEVQE. |
Gene expression databases | |
| ArrayExpress | Q9NZZ3. |
| Bgee | Q9NZZ3. |
| CleanEx | HS_CHMP5. |
| GermOnline | ENSG00000086065. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005024. Snf7. [Graphical view] |
| Pfam | PF03357. Snf7. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 55198. |
| SOURCE | Search... |
Entry information
| Entry name | CHMP5_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q9NZZ3 Secondary accession number(s): Q5VXW2 Q9Y323 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 9 Human chromosome 9: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


