Charged multivesicular body protein 5

Names and origin

Protein names

Recommended name:
    Charged multivesicular body protein 5
Alternative name(s):
    Chromatin-modifying protein 5
    Vacuolar protein-sorting-associated protein 60
      Short name=Vps60
      Short name=hVps60
    SNF7 domain-containing protein 2

Gene names

Name:	CHMP5
Synonyms:	C9orf83, SNF7DC2
ORF Names:	CGI-34, HSPC177, PNAS-2, PNAS-114

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. Ref.6
Subunit structure	Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III). ESCRT-III components are thought to multimerize to form a flat lattice on the perimeter membrane of the endosome. Several assembly forms of ESCRT-III may exist that interact and act sequentally. Interacts with VTA1. Interacts with CHMP2A. Interacts with VTA1; the interaction involves soluble CHMP5. Ref.6 Ref.8 Ref.10
Subcellular location	Cytoplasm › cytosol. Endosome membrane; Peripheral membrane protein Probable. Note: Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
Sequence similarities	Belongs to the SNF7 family.

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Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAMK2A	Q9UQM7	1	EBI-751303,EBI-1383687
STAMBP	O95630	1	EBI-751303,EBI-396676

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 219

219

Charged multivesicular body protein 5

PRO_0000211500

Regions

Region

121 – 158

38

Interaction with VTA1

Coiled coil

26 – 179

154

Potential

Amino acid modifications

Modified residue

219

1

Phosphoserine Ref.11

Natural variations

Natural variant

86

1

S → P: dbSNP rs11540558.

VAR_052029

Experimental info

Sequence conflict

14

1

P → R in AAD27743. Ref.1

Sequence conflict

19

1

D → G in AAH16698. Ref.4

Sequence conflict

84 – 93

10

QQSFNMEQAN → NSHSTWTGH in AAD27743. Ref.1

Sequence conflict

122

1

Q → P in AAD27743. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9NZZ3-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C99695F66FD7E126
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FASTA

219

24,571

        10         20         30         40         50         60 
MNRLFGKAKP KAPPPSLTDC IGTVDSRAES IDKKISRLDA ELVKYKDQIK KMREGPAKNM 

        70         80         90        100        110        120 
VKQKALRVLK QKRMYEQQRD NLAQQSFNME QANYTIQSLK DTKTTVDAMK LGVKEMKKAY 

       130        140        150        160        170        180 
KQVKIDQIED LQDQLEDMME DANEIQEALS RSYGTPELDE DDLEAELDAL GDELLADEDS 

       190        200        210 
SYLDEAASAP AIPEGVPTDT KNKDGVLVDE FGLPQIPAS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics." Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C. Genome Res. 10:703-713(2000) [PubMed: 10810093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow, Brain, Kidney and Urinary bladder.
[5]	"Human acute promyelocytic leukemia cell line NB4's apoptosis related genes." Yu W.-Q., Sun B.-Z., Chai Y.-B., Zhu F., Liu X.-S., Li Z., Lu F., Yan W., Yang H., Zhao Z.-L. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-219. Tissue: Promyelocytic leukemia.
[6]	"Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins." Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D. Proc. Natl. Acad. Sci. U.S.A. 100:12414-12419(2003) [PubMed: 14519844] [Abstract] Cited for: FUNCTION IN HIV-1 BUDDING, INTERACTION WITH CHMP2A.
[7]	Erratum Martin-Serrano J., Yarovoy A., Perez-Caballero D., Bieniasz P.D. Proc. Natl. Acad. Sci. U.S.A. 100:152845-152845(2003)
[8]	"The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells." Ward D.M., Vaughn M.B., Shiflett S.L., White P.L., Pollock A.L., Hill J., Schnegelberger R., Sundquist W.I., Kaplan J. J. Biol. Chem. 280:10548-10555(2005) [PubMed: 15644320] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH VTA1.
[9]	"Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis." Morita E., Sandrin V., Chung H.Y., Morham S.G., Gygi S.P., Rodesch C.K., Sundquist W.I. EMBO J. 26:4215-4227(2007) [PubMed: 17853893] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradation." Ma Y.M., Boucrot E., Villen J., Affar el B., Gygi S.P., Goettlinger H.G., Kirchhausen T. J. Biol. Chem. 282:9805-9812(2007) [PubMed: 17261583] [Abstract] Cited for: INTERACTION WITH VTA1, MASS SPECTROMETRY.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-219, MASS SPECTROMETRY.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

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Cross-references

Sequence databases
	AF132968 mRNA. Translation: AAD27743.1. AF161525 mRNA. Translation: AAF29140.1. AL356472 Genomic DNA. Translation: CAH72744.1. BC006974 mRNA. Translation: AAH06974.1. BC007457 mRNA. Translation: AAH07457.1. BC016698 mRNA. Translation: AAH16698.1. BC020796 mRNA. Translation: AAH20796.1. BC021168 mRNA. Translation: AAH21168.1. AF275810 mRNA. Translation: AAG23821.1. Different initiation. AF229832 mRNA. Translation: AAF42917.1.
IPI	IPI00100796.
RefSeq	NP_057494.3.
UniGene	Hs.635313
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NZZ3. 3 interactions.
PTM databases
PhosphoSite	Q9NZZ3.
Proteomic databases
PeptideAtlas	Q9NZZ3.
PRIDE	Q9NZZ3.
Genome annotation databases
Ensembl	ENSG00000086065. Homo sapiens. [Contig view]
GeneID	51510.
KEGG	hsa:51510.
UCSC	uc003zsl.2. human.
Organism-specific databases
GeneCards	GC09P033257.
H-InvDB	HIX0007979.
HGNC	HGNC:26942. CHMP5.
MIM	610900. gene.
PharmGKB	PA134903143.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NZZ3.
HOVERGEN	Q9NZZ3.
OMA	Q9NZZ3. QADEVQE.
Gene expression databases
ArrayExpress	Q9NZZ3.
Bgee	Q9NZZ3.
CleanEx	HS_CHMP5.
GermOnline	ENSG00000086065. Homo sapiens.
Family and domain databases
InterPro	IPR005024. Snf7. [Graphical view]
Pfam	PF03357. Snf7. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	55198.
SOURCE	Search...

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Entry information

Entry name

CHMP5_HUMAN

Accession

Primary (citable) accession number: Q9NZZ3
Secondary accession number(s): Q5VXW2

Q9Y323

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	October 1, 2000
Last modified:	July 7, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents