Reviewed, UniProtKB/Swiss-Prot Q9NZU7 (CABP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Calcium-binding protein 1
      Short name=CaBP1
Alternative name(s):
    Calbrain

Gene names

Name:

CABP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with MYO1C By similarity.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Cell membrane; Lipid-anchor; Cytoplasmic side. Note: L-CaBP1 is associated most likely with the cytoskeletal structures, whereas S-CaBP1 is localized at or near the plasma membrane.
Tissue specificity	Retina and brain. Calbrain was found exclusively in brain where it is abundant in the hippocampus, habenular area in the epithalamus and in the cerebellum.
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic density Inferred from direct assay. Source: UniProtKB
Molecular function	calcium ion binding Ref.2 Traceable author statement. Source: ProtInc calcium-dependent protein binding Inferred from physical interaction. Source: UniProtKB enzyme inhibitor activity Ref.2 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CACNA1C	Q13936	2	EBI-907894,EBI-1038838
CNP	P06623	1	EBI-907894,EBI-907934	From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform L-CaBP1 (identifier: Q9NZU7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform S-CaBP1 (identifier: Q9NZU7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     16-75: Missing.

Isoform Calbrain (identifier: Q9NZU7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.

Note: It is currently uncertain whether calbrain represent a spliced isoform.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 227	226	Calcium-binding protein 1		PRO_0000073513
Regions
	Domain	82 – 117	36	EF-hand 1
	Domain	118 – 153	36	EF-hand 2
	Domain	159 – 194	36	EF-hand 3
	Domain	196 – 227	32	EF-hand 4
	Calcium binding	95 – 106	12	1 Potential
	Calcium binding	172 – 183	12	2 Potential
	Calcium binding	209 – 220	12	3 Potential
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine
Natural variations
	Alternative sequence	1 – 157	157	Missing in isoform Calbrain.		VSP_000732
	Alternative sequence	16 – 75	60	Missing in isoform S-CaBP1.		VSP_000731

Sequences

	Sequence		Length	Mass (Da)
	Isoform L-CaBP1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FF3F7A9B575486AB	FASTA	227	25,979
	10 20 30 40 50 60 MGNCVKYPLR NLSRKMCQEE QTSYMVVQTS EEGLAADAEL PGPLLMLAQN CAVMHNLLGP 70 80 90 100 110 120 ACIFLRKGFA ENRQPDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE 130 140 150 160 170 180 MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS 190 200 210 220 TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR
	Isoform S-CaBP1. Checksum: B8C19D9E24D384FD	FASTA	167	19,430
	10 20 30 40 50 60 MGNCVKYPLR NLSRKDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE 70 80 90 100 110 120 MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS 130 140 150 160 TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR
	Isoform Calbrain. Checksum: 2807BEB35ADCDA48	FASTA	70	8,139
	10 20 30 40 50 60 MIGVKELRDA FREFDTNGDG EISTSELREA MRKLLGHQVG HRDIEEIIRD VDLNGDGRVD 70 FEEFVRMMSR

References

[1]	"Five members of a novel Ca(2+)-binding protein (CABP) subfamily with similarity to calmodulin." Haeseleer F., Sokal I., Verlinde C.L.M.J., Erdjument-Bromage H., Tempst P., Pronin A.N., Benovic J.L., Fariss R.N., Palczewski K. J. Biol. Chem. 275:1247-1260(2000) [PubMed: 10625670] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L-CABP1 AND S-CABP1), MYRISTOYLATION AT GLY-2. Tissue: Retina.
[2]	"Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulin-dependent protein kinase II activity in the brain." Yamaguchi K., Yamaguchi F., Miyamoto O., Sugimoto K., Konishi R., Hatase O. J. Biol. Chem. 274:3610-3616(1999) [PubMed: 9920909] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CALBRAIN). Tissue: Cerebellum.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF169149 mRNA. Translation: AAF25783.1.
AF169148 mRNA. Translation: AAF25782.1.
X94700 mRNA. Translation: CAA64361.1.

IPI

IPI00003571.
IPI00220810.
IPI00472586.

RefSeq

NP_112482.1.

UniGene

Hs.458482

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2K7B	NMR	-	A	76-151	[»]
2K7C	NMR	-	A	156-227	[»]
2K7D	NMR	-	A	156-227	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NZU7. 2 interactions.

Proteomic databases

PRIDE

Q9NZU7.

Genome annotation databases

Ensembl

ENSG00000157782. Homo sapiens. [Contig view]

GeneID

9478.

Organism-specific databases

GeneCards

GC12P119541.

H-InvDB

HIX0011074.

HGNC

HGNC:1384. CABP1.

MIM

605563. gene.

PharmGKB

PA26000.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9NZU7.

OMA

Q9NZU7. ENRQPDR.

Gene expression databases

ArrayExpress

Q9NZU7.

Bgee

Q9NZU7.

CleanEx

HS_CABP1.

GermOnline

ENSG00000157782. Homo sapiens.

Family and domain databases

InterPro

IPR015754. Ca_binding_pro.
IPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

PANTHER

PTHR23050:SF21. Ca_binding_pro. 1 hit.

Pfam

PF00036. efhand. 3 hits.
[Graphical view]

ProDom

PD000012. EF-hand. 2 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

35520.

SOURCE

Search...

Entry information

Entry name

CABP1_HUMAN

Accession

Primary (citable) accession number: Q9NZU7
Secondary accession number(s): O95663, Q9NZU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)