Q9NZU5 (LMCD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LIM and cysteine-rich domains protein 1

Alternative name(s):
Dyxin

Gene names

Name:

LMCD1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter By similarity. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T cells signaling pathway. Ref.6
Subunit structure	Interacts with GATA1 and GATA4 By similarity. Interacts with beta-dystroglycan. Interacts with GATA6. Ref.5
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: May shuttle between the cytoplasm and the nucleus By similarity.
Tissue specificity	Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle. Ref.6
Domain	The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6 By similarity.
Sequence similarities	Contains 2 LIM zinc-binding domains. Contains 1 PET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	LIM domain Repeat
Ligand	Metal-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of calcineurin-NFAT signaling pathway Inferred from mutant phenotype Ref.6. Source: UniProtKB regulation of cardiac muscle hypertrophy Inferred from mutant phenotype Ref.6. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from direct assay. Source: BHF-UCL nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription corepressor activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	LIM and cysteine-rich domains protein 1		PRO_0000075815
Regions
	Domain	99 – 206	108	PET
	Domain	241 – 306	66	LIM zinc-binding 1
	Domain	307 – 365	59	LIM zinc-binding 2
	Compositional bias	28 – 58	31	Cys-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9NZU5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D84BFD53C43D2DD7	FASTA	365	40,833
	10 20 30 40 50 60 MAKVAKDLNP GVKKMSLGQL QSARGVACLG CKGTCSGFEP HSWRKICKSC KCSQEDHCLT 70 80 90 100 110 120 SDLEDDRKIG RLLMDSKYST LTARVKGGDG IRIYKRNRMI MTNPIATGKD PTFDTITYEW 130 140 150 160 170 180 APPGVTQKLG LQYMELIPKE KQPVTGTEGA FYRRRQLMHQ LPIYDQDPSR CRGLLENELK 190 200 210 220 230 240 LMEEFVKQYK SEALGVGEVA LPGQGGLPKE EGKQQEKPEG AETTAATTNG SLSDPSKEVE 250 260 270 280 290 300 YVCELCKGAA PPDSPVVYSD RAGYNKQWHP TCFVCAKCSE PLVDLIYFWK DGAPWCGRHY 310 320 330 340 350 360 CESLRPRCSG CDEIIFAEDY QRVEDLAWHR KHFVCEGCEQ LLSGRAYIVT KGQLLCPTCS KSKRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel LIM domain gene, LMCD1, and chromosomal localization in human and mouse." Bespalova I.N., Burmeister M. Genomics 63:69-74(2000) [PubMed: 10662546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of Dyxin: a novel intracellular binding partner of beta-dystroglycan." Holt H.H., Crosbie R.H., Saito F., Campbell K.P. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[5]	"LMCD1/Dyxin is a novel transcriptional cofactor that restricts GATA6 function by inhibiting DNA binding." Rath N., Wang Z., Lu M.M., Morrisey E.E. Mol. Cell. Biol. 25:8864-8873(2005) [PubMed: 16199866] [Abstract] Cited for: INTERACTION WITH GATA6.
[6]	"LIM and cysteine-rich domains 1 regulates cardiac hypertrophy by targeting calcineurin/nuclear factor of activated T cells signaling." Bian Z.Y., Huang H., Jiang H., Shen D.F., Yan L., Zhu L.H., Wang L., Cao F., Liu C., Tang Q.Z., Li H. Hypertension 55:257-263(2010) [PubMed: 20026769] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF169284 mRNA. Translation: AAF34411.1. AF216709 mRNA. Translation: AAG36778.1. AK022176 mRNA. Translation: BAB13976.1. BC000646 mRNA. Translation: AAH00646.1.
IPI	IPI00303258.
RefSeq	NP_055398.1. NM_014583.2.
UniGene	Hs.475353.
3D structure databases
ProteinModelPortal	Q9NZU5.
SMR	Q9NZU5. Positions 240-365.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6492974.
STRING	Q9NZU5.
Polymorphism databases
DMDM	20978521.
Proteomic databases
PeptideAtlas	Q9NZU5.
PRIDE	Q9NZU5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000157600; ENSP00000157600; ENSG00000071282.
GeneID	29995.
KEGG	hsa:29995.
UCSC	uc003bqq.1. human.
Organism-specific databases
CTD	29995.
GeneCards	GC03P008518.
H-InvDB	HIX0003018.
HGNC	HGNC:6633. LMCD1.
HPA	HPA024059.
MIM	604859. gene.
neXtProt	NX_Q9NZU5.
PharmGKB	PA30401.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06308.
GeneTree	ENSGT00550000074438.
HOGENOM	HBG716344.
HOVERGEN	HBG001038.
InParanoid	Q9NZU5.
OMA	LSGRAYI.
OrthoDB	EOG45B1G6.
PhylomeDB	Q9NZU5.
Gene expression databases
ArrayExpress	Q9NZU5.
Bgee	Q9NZU5.
CleanEx	HS_LMCD1.
Genevestigator	Q9NZU5.
GermOnline	ENSG00000071282. Homo sapiens.
Family and domain databases
InterPro	IPR010442. PET_domain. IPR001781. Znf_LIM. [Graphical view]
Gene3D	G3DSA:2.10.110.10. Znf_LIM. 2 hits.
Pfam	PF00412. LIM. 2 hits. PF06297. PET. 1 hit. [Graphical view]
SMART	SM00132. LIM. 2 hits. [Graphical view]
PROSITE	PS00478. LIM_DOMAIN_1. 2 hits. PS50023. LIM_DOMAIN_2. 2 hits. PS51303. PET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	52796.
SOURCE	Search...

Entry information

Entry name

LMCD1_HUMAN

Accession

Primary (citable) accession number: Q9NZU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.