Reviewed, UniProtKB/Swiss-Prot Q9NZT1 (CALL5_HUMAN)

Names and origin

Protein names

Recommended name:
    Calmodulin-like protein 5
Alternative name(s):
    Calmodulin-like skin protein

Gene names

Name:	CALML5
Synonyms:	CLSP

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds calcium. May be involved in terminal differentiation of keratinocytes.
Subunit structure	Associates with transglutaminase 3.
Tissue specificity	Particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation. Very low expression in lung.
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calcium
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	epidermis development Ref.1 Traceable author statement. Source: ProtInc signal transduction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	calcium ion binding Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MAGED1	Q9Y5V3	1	EBI-1051681,EBI-716006
YWHAQ	P27348	1	EBI-1051681,EBI-359854

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.4

Chain

2 – 146

145

Calmodulin-like protein 5

PRO_0000073854

Regions

Domain

8 – 43

36

EF-hand 1

Domain

44 – 77

34

EF-hand 2

Domain

78 – 113

36

EF-hand 3

Domain

114 – 146

33

EF-hand 4

Calcium binding

21 – 32

12

1 Ref.5

Calcium binding

57 – 68

12

2 Potential

Calcium binding

91 – 102

12

3 Potential

Calcium binding

127 – 138

12

4 Potential

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.4

Natural variations

Natural variant

58

1

S → G: dbSNP rs11546426. Ref.3

VAR_047545

Natural variant

74

1

K → R: dbSNP rs10904516. Ref.3 Ref.1

VAR_047546

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

146

Helix Strand Turn

Details...

Helix

80 – 87

8

Beta strand

93 – 99

7

Helix

100 – 106

7

Helix

107 – 109

3

Helix

116 – 125

10

Beta strand

128 – 135

8

Helix

138 – 143

6

Sequences

	Sequence		Length	Mass (Da)
	Q9NZT1-1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: 70746291268494CC	FASTA	146	15,893
	10 20 30 40 50 60 MAGELTPEEE AQYKKAFSAV DTDGNGTINA QELGAALKAT GKNLSEAQLR KLISEVDSDG 70 80 90 100 110 120 DGEISFQEFL TAAKKARAGL EDLQVAFRAF DQDGDGHITV DELRRAMAGL GQPLPQEELD 130 140 AMIREADVDQ DGRVNYEEFA RMLAQE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and cloning of a new calmodulin-like protein from human epidermis." Mehul B., Bernard D., Simonetti L., Bernard M.A., Schmidt R. J. Biol. Chem. 275:12841-12847(2000) [PubMed: 10777582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, VARIANT ARG-74. Tissue: Skin.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLY-58 AND ARG-74. Tissue: Skin.
[4]	Bienvenut W.V., Dhillon A.S., Kolch W. Submitted (FEB-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-50, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Hepatoma.
[5]	"A structural and dynamic characterization of the EF-hand protein CLSP." Babini E., Bertini I., Capozzi F., Chirivino E., Luchinat C. Structure 14:1029-1038(2006) [PubMed: 16765896] [Abstract] Cited for: STRUCTURE BY NMR OF 76-146, CALCIUM-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF172852 mRNA. Translation: AAF66821.1.
AL732437 Genomic DNA. Translation: CAI11030.1.
BC039172 mRNA. Translation: AAH39172.1.

IPI

IPI00021536.

RefSeq

NP_059118.2.

UniGene

Hs.180142

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B1U	NMR	-	A	76-146	[»]

SMR

Q9NZT1. Positions 2-74, 4-142.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NZT1. 5 interactions.

STRING

Q9NZT1.

PTM databases

PhosphoSite

Q9NZT1.

Genome annotation databases

Ensembl

ENST00000380332; ENSP00000369689; ENSG00000178372; Homo sapiens. [Genome view]

GeneID

51806.

KEGG

hsa:51806.

UCSC

uc001iic.1. human.

Organism-specific databases

CTD

51806.

GeneCards

GC10M005530.

H-InvDB

HIX0025916.

HGNC

HGNC:18180. CALML5.

MIM

605183. gene.

PharmGKB

PA134862009.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

HBG746798.

HOVERGEN

Q9NZT1.

InParanoid

Q9NZT1.

OMA

GTINAQE.

OrthoDB

EOG9Z657F.

PhylomeDB

Q9NZT1.

Gene expression databases

ArrayExpress

Q9NZT1.

Bgee

Q9NZT1.

CleanEx

HS_CALML5.

Genevestigator

Q9NZT1.

GermOnline

ENSG00000178372. Homo sapiens.

Family and domain databases

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

SMART

SM00054. EFh. 4 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

55928.

SOURCE

Search...

Entry information

Entry name

CALL5_HUMAN

Accession

Primary (citable) accession number: Q9NZT1
Secondary accession number(s): Q5SQI3, Q8IXU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 25, 2008
Last modified:	February 9, 2010
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.