Reviewed, UniProtKB/Swiss-Prot Q9NZS9 (BFAR_HUMAN)

Names and origin

Protein names

Recommended name:
    Bifunctional apoptosis regulator
Alternative name(s):
    RING finger protein 47

Gene names

Name:	BFAR
Synonyms:	BAR, RNF47

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. Ref.3
Subunit structure	Interacts with CASP8, BCL2 and BCL2L1 through SAM domain and also with HIP1, IFT57, ESRRBL1 and BCAP31. Ref.3 Ref.1
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Ref.3 Ref.1
Tissue specificity	Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen. Ref.3 Ref.1
Sequence similarities	Contains 1 RING-type zinc finger. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	anti-apoptosis Ref.1 Inferred from direct assay. Source: MGI apoptosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc membrane fraction Ref.1 Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro structural molecule activity Ref.1 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Bifunctional apoptosis regulator		PRO_0000055822
Regions
	Topological domain	1 – 140	140	Cytoplasmic Potential
	Transmembrane	141 – 161	21	Potential
	Topological domain	162 – 331	170	Lumenal Potential
	Transmembrane	332 – 352	21	Potential
	Topological domain	353 – 360	8	Cytoplasmic Potential
	Transmembrane	361 – 381	21	Potential
	Topological domain	382 – 404	23	Lumenal Potential
	Transmembrane	405 – 425	21	Potential
	Topological domain	426 – 450	25	Cytoplasmic Potential
	Domain	182 – 249	68	SAM
	Zinc finger	34 – 74	41	RING-type
Amino acid modifications
	Modified residue	376	1	Phosphoserine Ref.4
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	140	1	M → R: dbSNP rs11546303.		VAR_052075
	Natural variant	245	1	R → H: dbSNP rs35377618.		VAR_052076

Sequences

	Sequence		Length	Mass (Da)
	Q9NZS9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A662454FC2806CE6	FASTA	450	52,738
	10 20 30 40 50 60 MEEPQKSYVN TMDLERDEPL KSTGPQISVS EFSCHCCYDI LVNPTTLNCG HSFCRHCLAL 70 80 90 100 110 120 WWASSKKTEC PECREKWEGF PKVSILLRDA IEKLFPDAIR LRFEDIQQNN DIVQSLAAFQ 130 140 150 160 170 180 KYGNDQIPLA PNTGRANQQM GGGFFSGVLT ALTGVAVVLL VYHWSSRESE HDLLVHKAVA 190 200 210 220 230 240 KWTAEEVVLW LEQLGPWASL YRERFLSERV NGRLLLTLTE EEFSKTPYTI ENSSHRRAIL 250 260 270 280 290 300 MELERVKALG VKPPQNLWEY KAVNPGRSLF LLYALKSSPR LSLLYLYLFD YTDTFLPFIH 310 320 330 340 350 360 TICPLQEDSS GEDIVTKLLD LKEPTWKQWR EFLVKYSFLP YQLIAEFAWD WLEVHYWTSR 370 380 390 400 410 420 FLIINAMLLS VLELFSFWRI WSRSELKTVP QRMWSHFWKV STQGLFVAMF WPLIPQFVCN 430 440 450 CLFYWALYFN PIINIDLVVK ELRRLETQVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BAR: an apoptosis regulator at the intersection of caspases and Bcl-2 family proteins." Zhang H., Xu Q., Krajewski S., Krajewska M., Xie Z., Fuess S., Kitada S., Pawlowski K., Godzik A., Reed J.C. Proc. Natl. Acad. Sci. U.S.A. 97:2597-2602(2000) [PubMed: 10716992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CASP8; BCL2 AND BCL2L1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Hepatoma.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways." Roth W., Kermer P., Krajewska M., Welsh K., Davis S., Krajewski S., Reed J.C. Cell Death Differ. 10:1178-1187(2003) [PubMed: 14502241] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH IFT57; HIP1; ESRRBL1 AND BCAP31, TISSUE SPECIFICITY.
[4]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF173003 mRNA. Translation: AAF59975.1. BC003054 mRNA. Translation: AAH03054.1.
IPI	IPI00009811.
RefSeq	NP_057645.1.
UniGene	Hs.435556
3D structure databases
SMR	Q9NZS9. Positions 170-248.
ModBase	Search...
PTM databases
PhosphoSite	Q9NZS9.
Proteomic databases
PRIDE	Q9NZS9.
Genome annotation databases
Ensembl	ENSG00000103429. Homo sapiens. [Contig view]
GeneID	51283.
KEGG	hsa:51283.
Organism-specific databases
GeneCards	GC16P014634.
H-InvDB	HIX0012831.
HGNC	HGNC:17613. BFAR.
PharmGKB	PA38460.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NZS9.
HOVERGEN	Q9NZS9.
OMA	Q9NZS9. VAKWTAE.
Gene expression databases
ArrayExpress	Q9NZS9.
Bgee	Q9NZS9.
CleanEx	HS_BFAR.
GermOnline	ENSG00000103429. Homo sapiens.
Family and domain databases
InterPro	IPR001660. SAM. IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF00536. SAM_1. 1 hit. PF00097. zf-C3HC4. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
PROSITE	PS50105. SAM_DOMAIN. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	54523.

Entry information

Entry name

BFAR_HUMAN

Accession

Primary (citable) accession number: Q9NZS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)