Q9NZS9 (BFAR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional apoptosis regulator

Alternative name(s):
RING finger protein 47

Gene names

Name:	BFAR
Synonyms:	BAR, RNF47

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Apoptosis regulator. Has anti-apoptotic activity, both for apoptosis triggered via death-receptors and via mitochondrial factors. Ref.5
Subunit structure	Interacts with CASP8, BCL2 and BCL2L1 through SAM domain and also with HIP1, IFT57, ESRRBL1 and BCAP31. Ref.1 Ref.5
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1 Ref.5.
Tissue specificity	Expressed highly in brain, moderately in small intestine, weakly in testes and only faintly in liver and skeletal muscle. Not expressed in heart, kidney, lung and spleen. Ref.1 Ref.5
Sequence similarities	Contains 1 RING-type zinc finger. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anti-apoptosis Inferred from direct assay Ref.1. Source: MGI apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement. Source: ProtInc membrane fraction Inferred from direct assay Ref.1. Source: MGI
Molecular function	structural molecule activity Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Bifunctional apoptosis regulator		PRO_0000055822
Regions
	Topological domain	1 – 140	140	Cytoplasmic Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Topological domain	162 – 331	170	Lumenal Potential
	Transmembrane	332 – 352	21	Helical; Potential
	Topological domain	353 – 360	8	Cytoplasmic Potential
	Transmembrane	361 – 381	21	Helical; Potential
	Topological domain	382 – 404	23	Lumenal Potential
	Transmembrane	405 – 425	21	Helical; Potential
	Topological domain	426 – 450	25	Cytoplasmic Potential
	Domain	182 – 249	68	SAM
	Zinc finger	34 – 74	41	RING-type
Amino acid modifications
	Modified residue	376	1	Phosphoserine Ref.6
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	140	1	M → R. Corresponds to variant rs11546303 [ dbSNP | Ensembl ].		VAR_052075
	Natural variant	245	1	R → H. Corresponds to variant rs35377618 [ dbSNP | Ensembl ].		VAR_052076

Sequences

	Sequence		Length	Mass (Da)
	Q9NZS9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A662454FC2806CE6	FASTA	450	52,738
	10 20 30 40 50 60 MEEPQKSYVN TMDLERDEPL KSTGPQISVS EFSCHCCYDI LVNPTTLNCG HSFCRHCLAL 70 80 90 100 110 120 WWASSKKTEC PECREKWEGF PKVSILLRDA IEKLFPDAIR LRFEDIQQNN DIVQSLAAFQ 130 140 150 160 170 180 KYGNDQIPLA PNTGRANQQM GGGFFSGVLT ALTGVAVVLL VYHWSSRESE HDLLVHKAVA 190 200 210 220 230 240 KWTAEEVVLW LEQLGPWASL YRERFLSERV NGRLLLTLTE EEFSKTPYTI ENSSHRRAIL 250 260 270 280 290 300 MELERVKALG VKPPQNLWEY KAVNPGRSLF LLYALKSSPR LSLLYLYLFD YTDTFLPFIH 310 320 330 340 350 360 TICPLQEDSS GEDIVTKLLD LKEPTWKQWR EFLVKYSFLP YQLIAEFAWD WLEVHYWTSR 370 380 390 400 410 420 FLIINAMLLS VLELFSFWRI WSRSELKTVP QRMWSHFWKV STQGLFVAMF WPLIPQFVCN 430 440 450 CLFYWALYFN PIINIDLVVK ELRRLETQVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BAR: an apoptosis regulator at the intersection of caspases and Bcl-2 family proteins." Zhang H., Xu Q., Krajewski S., Krajewska M., Xie Z., Fuess S., Kitada S., Pawlowski K., Godzik A., Reed J.C. Proc. Natl. Acad. Sci. U.S.A. 97:2597-2602(2000) [PubMed: 10716992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CASP8; BCL2 AND BCL2L1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Hepatoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways." Roth W., Kermer P., Krajewska M., Welsh K., Davis S., Krajewski S., Reed J.C. Cell Death Differ. 10:1178-1187(2003) [PubMed: 14502241] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH IFT57; HIP1; ESRRBL1 AND BCAP31, TISSUE SPECIFICITY.
[6]	"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column." Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y. Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF173003 mRNA. Translation: AAF59975.1. AK291114 mRNA. Translation: BAF83803.1. CH471112 Genomic DNA. Translation: EAW85105.1. CH471112 Genomic DNA. Translation: EAW85108.1. BC003054 mRNA. Translation: AAH03054.1.
IPI	IPI00009811.
RefSeq	NP_057645.1. NM_016561.2.
UniGene	Hs.435556.
3D structure databases
ProteinModelPortal	Q9NZS9.
SMR	Q9NZS9. Positions 26-110, 170-248.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NZS9. 9 interactions.
STRING	Q9NZS9.
PTM databases
PhosphoSite	Q9NZS9.
Polymorphism databases
DMDM	74753089.
Proteomic databases
PRIDE	Q9NZS9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261658; ENSP00000261658; ENSG00000103429.
GeneID	51283.
KEGG	hsa:51283.
UCSC	uc002dco.1. human.
Organism-specific databases
CTD	51283.
GeneCards	GC16P014634.
H-InvDB	HIX0012831.
HGNC	HGNC:17613. BFAR.
neXtProt	NX_Q9NZS9.
PharmGKB	PA38460.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09908.
GeneTree	ENSGT00390000005386.
HOGENOM	HBG714291.
HOVERGEN	HBG056789.
InParanoid	Q9NZS9.
OMA	FEDIQQN.
OrthoDB	EOG4N5VWT.
PhylomeDB	Q9NZS9.
Gene expression databases
ArrayExpress	Q9NZS9.
Bgee	Q9NZS9.
CleanEx	HS_BFAR.
Genevestigator	Q9NZS9.
GermOnline	ENSG00000103429. Homo sapiens.
Family and domain databases
InterPro	IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. IPR018957. Znf_C3HC4_RING-type. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
Gene3D	G3DSA:1.10.150.50. SAM_type. 1 hit. G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
Pfam	PF00536. SAM_1. 1 hit. PF00097. zf-C3HC4. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS50105. SAM_DOMAIN. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	54523.

Entry information

Entry name

BFAR_HUMAN

Accession

Primary (citable) accession number: Q9NZS9
Secondary accession number(s): A8K4Z9, D3DUG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.