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Protein

Killer cell lectin-like receptor subfamily F member 1

Gene

KLRF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the natural killer (NK)-mediated cytolysis of PHA-induced lymphoblasts.

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • MHC class I receptor activity Source: UniProtKB
  • transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

  • cell surface receptor signaling pathway Source: ProtInc
  • regulation of immune response Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Lectin

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
Killer cell lectin-like receptor subfamily F member 1
Short name:
Lectin-like receptor F1
Alternative name(s):
Activating coreceptor NKp80
C-type lectin domain family 5 member C
Gene namesi
Name:KLRF1
Synonyms:CLEC5C, ML
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:13342. KLRF1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3838CytoplasmicSequence analysisAdd
BLAST
Transmembranei39 – 5921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini60 – 232173ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: ProtInc
  • membrane Source: UniProtKB
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30169.

Polymorphism and mutation databases

BioMutaiKLRF1.
DMDMi317373373.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Killer cell lectin-like receptor subfamily F member 1PRO_0000046591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence analysis
Disulfide bondi143 ↔ 230PROSITE-ProRule annotation
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence analysis
Disulfide bondi209 ↔ 222PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NZS2.
PRIDEiQ9NZS2.

PTM databases

iPTMnetiQ9NZS2.
PhosphoSiteiQ9NZS2.

Expressioni

Tissue specificityi

Strongly expressed in peripheral blood leukocytes and spleen, with weaker expression in lymph node and adult liver, and no expression detected in bone marrow, thymus, and fetal liver. Not expressed in brain, heart, placenta, lung, kidney, skeletal muscle, and pancreas. Within peripheral blood leukocyte and immunocyte cell lines, expression was predominant in NK cells but was also detected in monocytes.1 Publication

Gene expression databases

BgeeiQ9NZS2.
CleanExiHS_KLRF1.
ExpressionAtlasiQ9NZS2. baseline and differential.
GenevisibleiQ9NZS2. HS.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi119491. 6 interactions.
DIPiDIP-58613N.
STRINGi9606.ENSP00000279544.

Structurei

3D structure databases

ProteinModelPortaliQ9NZS2.
SMRiQ9NZS2. Positions 114-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini121 – 231111C-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00730000110837.
HOGENOMiHOG000285951.
HOVERGENiHBG052268.
InParanoidiQ9NZS2.
KOiK10075.
OrthoDBiEOG7PVWQ9.
PhylomeDBiQ9NZS2.
TreeFamiTF337735.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZS2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQDEERYMTL NVQSKKRSSA QTSQLTFKDY SVTLHWYKIL LGISGTVNGI
60 70 80 90 100
LTLTLISLIL LVSQGVLLKC QKGSCSNATQ YEDTGDLKVN NGTRRNISNK
110 120 130 140 150
DLCASRSADQ TVLCQSEWLK YQGKCCYWFS NEMKSWSDSY VYCLERKSHL
160 170 180 190 200
LIIHDQLEMA FIQKNLRQLN YVWIGLNFTS LKMTWTWVDG SPIDSKIFFI
210 220 230
KGPAKENSCA AIKESKIFSE TCSSVFKWIC QY
Length:232
Mass (Da):26,666
Last modified:January 11, 2011 - v2
Checksum:iF5DD21F72E1E8C72
GO
Isoform 2 (identifier: Q9NZS2-2) [UniParc]FASTAAdd to basket

Also known as: KLRF1-s1

The sequence of this isoform differs from the canonical sequence as follows:
     63-112: Missing.

Show »
Length:182
Mass (Da):21,308
Checksum:i248BB26B03972C38
GO
Isoform 3 (identifier: Q9NZS2-3) [UniParc]FASTAAdd to basket

Also known as: KLRF1-s3

The sequence of this isoform differs from the canonical sequence as follows:
     62-64: VSQ → DSS
     65-232: Missing.

Show »
Length:64
Mass (Da):7,251
Checksum:iC451F5F560FD75D7
GO
Isoform 4 (identifier: Q9NZS2-4) [UniParc]FASTAAdd to basket

Also known as: KLRF1-s2

The sequence of this isoform differs from the canonical sequence as follows:
     62-78: VSQGVLLKCQKGSCSNA → GFYTEKPKTIKLRMDWA
     79-232: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:78
Mass (Da):9,029
Checksum:i3DE810CF5656D634
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251Missing in AAF37804 (PubMed:10671213).Curated
Sequence conflicti125 – 1251Missing in AAF37805 (PubMed:10671213).Curated
Sequence conflicti125 – 1251Missing in CAC29425 (PubMed:11265639).Curated
Sequence conflicti125 – 1251Missing in AAH98354 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti67 – 671L → F.
Corresponds to variant rs2232548 [ dbSNP | Ensembl ].
VAR_047544

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei62 – 7817VSQGV…SCSNA → GFYTEKPKTIKLRMDWA in isoform 4. 1 PublicationVSP_010393Add
BLAST
Alternative sequencei62 – 643VSQ → DSS in isoform 3. 1 PublicationVSP_010390
Alternative sequencei63 – 11250Missing in isoform 2. 1 PublicationVSP_010391Add
BLAST
Alternative sequencei65 – 232168Missing in isoform 3. 1 PublicationVSP_010392Add
BLAST
Alternative sequencei79 – 232154Missing in isoform 4. 1 PublicationVSP_010394Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175206 mRNA. Translation: AAF37804.1.
AF175207 mRNA. Translation: AAF37805.1.
AF267244 mRNA. Translation: AAL14873.1.
AF267245 mRNA. Translation: AAL14874.1.
AJ305370 mRNA. Translation: CAC29425.1.
AC007068 Genomic DNA. No translation available.
BC098354 mRNA. Translation: AAH98354.1.
CCDSiCCDS76527.1. [Q9NZS2-3]
RefSeqiNP_001278751.1. NM_001291822.1.
NP_001278752.1. NM_001291823.1. [Q9NZS2-3]
NP_057607.1. NM_016523.2.
UniGeneiHs.183125.

Genome annotation databases

EnsembliENST00000354855; ENSP00000346919; ENSG00000150045. [Q9NZS2-3]
ENST00000612321; ENSP00000483880; ENSG00000150045. [Q9NZS2-4]
GeneIDi51348.
KEGGihsa:51348.
UCSCiuc009zgy.4. human. [Q9NZS2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

KLRF1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175206 mRNA. Translation: AAF37804.1.
AF175207 mRNA. Translation: AAF37805.1.
AF267244 mRNA. Translation: AAL14873.1.
AF267245 mRNA. Translation: AAL14874.1.
AJ305370 mRNA. Translation: CAC29425.1.
AC007068 Genomic DNA. No translation available.
BC098354 mRNA. Translation: AAH98354.1.
CCDSiCCDS76527.1. [Q9NZS2-3]
RefSeqiNP_001278751.1. NM_001291822.1.
NP_001278752.1. NM_001291823.1. [Q9NZS2-3]
NP_057607.1. NM_016523.2.
UniGeneiHs.183125.

3D structure databases

ProteinModelPortaliQ9NZS2.
SMRiQ9NZS2. Positions 114-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119491. 6 interactions.
DIPiDIP-58613N.
STRINGi9606.ENSP00000279544.

PTM databases

iPTMnetiQ9NZS2.
PhosphoSiteiQ9NZS2.

Polymorphism and mutation databases

BioMutaiKLRF1.
DMDMi317373373.

Proteomic databases

PaxDbiQ9NZS2.
PRIDEiQ9NZS2.

Protocols and materials databases

DNASUi51348.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354855; ENSP00000346919; ENSG00000150045. [Q9NZS2-3]
ENST00000612321; ENSP00000483880; ENSG00000150045. [Q9NZS2-4]
GeneIDi51348.
KEGGihsa:51348.
UCSCiuc009zgy.4. human. [Q9NZS2-1]

Organism-specific databases

CTDi51348.
GeneCardsiKLRF1.
HGNCiHGNC:13342. KLRF1.
MIMi605029. gene.
neXtProtiNX_Q9NZS2.
PharmGKBiPA30169.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00730000110837.
HOGENOMiHOG000285951.
HOVERGENiHBG052268.
InParanoidiQ9NZS2.
KOiK10075.
OrthoDBiEOG7PVWQ9.
PhylomeDBiQ9NZS2.
TreeFamiTF337735.

Enzyme and pathway databases

ReactomeiR-HSA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

GenomeRNAii51348.
PROiQ9NZS2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NZS2.
CleanExiHS_KLRF1.
ExpressionAtlasiQ9NZS2. baseline and differential.
GenevisibleiQ9NZS2. HS.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human KLRF1, a novel member of the killer cell lectin-like receptor gene family: molecular characterization, genomic structure, physical mapping to the NK gene complex and expression analysis."
    Roda-Navarro P., Arce I., Renedo M., Montgomery K., Kucherlapati R., Fernandez-Ruiz E.
    Eur. J. Immunol. 30:568-576(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
  2. "Molecular characterization of two novel alternative spliced variants of the KLRF1 gene and subcellular distribution of KLRF1 isoforms."
    Roda-Navarro P., Hernanz-Falcon P., Arce I., Fernandez-Ruiz E.
    Biochim. Biophys. Acta 1520:141-146(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
  3. "Identification of NKp80, a novel triggering molecule expressed by human natural killer cells."
    Vitale M., Falco M., Castriconi R., Parolini S., Zambello R., Semenzato G., Biassoni R., Bottino C., Moretta L., Moretta A.
    Eur. J. Immunol. 31:233-242(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT.
    Tissue: Lymphoid tissue.
  4. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).

Entry informationi

Entry nameiKLRF1_HUMAN
AccessioniPrimary (citable) accession number: Q9NZS2
Secondary accession number(s): Q4KMT5
, Q96PR2, Q96PR3, Q9NZS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: January 11, 2011
Last modified: June 8, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.