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Q9NZQ8 (TRPM5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transient receptor potential cation channel subfamily M member 5
Alternative name(s):
Long transient receptor potential channel 5
Short name=LTrpC-5
Short name=LTrpC5
MLSN1- and TRP-related gene 1 protein
Gene names
Name:TRPM5
Synonyms:LTRPC5, MTR1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1165 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Voltage-modulated Ca2+-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na+, K+ and Cs+ ions equally well. Activated directly by increases in intracellular Ca2+, but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca2+. Also activated by a fast intracellular Ca2+ increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. Ref.5 UniProtKB Q9JJH7

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Tissue specificity

Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression. Ref.1

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM5 sub-subfamily. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 Ref.2 Ref.3 (identifier: Q9NZQ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.4 (identifier: Q9NZQ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     155-155: Q → QVH
Isoform 3 Ref.1 (identifier: Q9NZQ8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     870-872: MKD → KPV
     873-1165: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11651165Transient receptor potential cation channel subfamily M member 5
PRO_0000328933

Regions

Topological domain1 – 646646Cytoplasmic Potential
Transmembrane647 – 66721Helical; Potential
Topological domain668 – 73366Extracellular Potential
Transmembrane734 – 75421Helical; Potential
Topological domain755 – 81157Cytoplasmic Potential
Transmembrane812 – 83221Helical; Potential
Topological domain833 – 8353Extracellular Potential
Transmembrane836 – 85621Helical; Potential
Topological domain857 – 87216Cytoplasmic Potential
Transmembrane873 – 89321Helical; Potential
Topological domain894 – 95461Extracellular Potential
Transmembrane955 – 97521Helical; Potential
Topological domain976 – 1165190Cytoplasmic Potential
Coiled coil553 – 58129 Potential

Natural variations

Alternative sequence1551Q → QVH in isoform 2. Ref.4
VSP_052741
Alternative sequence870 – 8723MKD → KPV in isoform 3. Ref.1
VSP_052742
Alternative sequence873 – 1165293Missing in isoform 3. Ref.1
VSP_052743
Natural variant2351N → S.
Corresponds to variant rs886277 [ dbSNP | Ensembl ].
VAR_052377
Natural variant2541V → A. Ref.3
Corresponds to variant rs3986599 [ dbSNP | Ensembl ].
VAR_042578
Natural variant3351V → L.
Corresponds to variant rs34350821 [ dbSNP | Ensembl ].
VAR_052378
Natural variant4561A → T.
Corresponds to variant rs34551253 [ dbSNP | Ensembl ].
VAR_052379
Natural variant5781R → Q.
Corresponds to variant rs4929982 [ dbSNP | Ensembl ].
VAR_059836

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: C4AD5BAA866BE73B

FASTA1,165131,451
        10         20         30         40         50         60 
MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW 

        70         80         90        100        110        120 
HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV 

       130        140        150        160        170        180 
RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQEDFPV HYPEDDGGSQ GPLCSLDSNL 

       190        200        210        220        230        240 
SHFILVEPGP PGKGDGLTEL RLRLEKHISE QRAGYGGTGS IEIPVLCLLV NGDPNTLERI 

       250        260        270        280        290        300 
SRAVEQAAPW LILVGSGGIA DVLAALVNQP HLLVPKVAEK QFKEKFPSKH FSWEDIVRWT 

       310        320        330        340        350        360 
KLLQNITSHQ HLLTVYDFEQ EGSEELDTVI LKALVKACKS HSQEPQDYLD ELKLAVAWDR 

       370        380        390        400        410        420 
VDIAKSEIFN GDVEWKSCDL EEVMVDALVS NKPEFVRLFV DNGADVADFL TYGRLQELYR 

       430        440        450        460        470        480 
SVSRKSLLFD LLQRKQEEAR LTLAGLGTQQ AREPPAGPPA FSLHEVSRVL KDFLQDACRG 

       490        500        510        520        530        540 
FYQDGRPGDR RRAEKGPAKR PTGQKWLLDL NQKSENPWRD LFLWAVLQNR HEMATYFWAM 

       550        560        570        580        590        600 
GQEGVAAALA ACKILKEMSH LETEAEAARA TREAKYERLA LDLFSECYSN SEARAFALLV 

       610        620        630        640        650        660 
RRNRCWSKTT CLHLATEADA KAFFAHDGVQ AFLTRIWWGD MAAGTPILRL LGAFLCPALV 

       670        680        690        700        710        720 
YTNLITFSEE APLRTGLEDL QDLDSLDTEK SPLYGLQSRV EELVEAPRAQ GDRGPRAVFL 

       730        740        750        760        770        780 
LTRWRKFWGA PVTVFLGNVV MYFAFLFLFT YVLLVDFRPP PQGPSGPEVT LYFWVFTLVL 

       790        800        810        820        830        840 
EEIRQGFFTD EDTHLVKKFT LYVGDNWNKC DMVAIFLFIV GVTCRMLPSA FEAGRTVLAM 

       850        860        870        880        890        900 
DFMVFTLRLI HIFAIHKQLG PKIIVVERMM KDVFFFLFFL SVWLVAYGVT TQALLHPHDG 

       910        920        930        940        950        960 
RLEWIFRRVL YRPYLQIFGQ IPLDEIDEAR VNCSTHPLLL EDSPSCPSLY ANWLVILLLV 

       970        980        990       1000       1010       1020 
TFLLVTNVLL MNLLIAMFSY TFQVVQGNAD MFWKFQRYNL IVEYHERPAL APPFILLSHL 

      1030       1040       1050       1060       1070       1080 
SLTLRRVFKK EAEHKREHLE RDLPDPLDQK VVTWETVQKE NFLSKMEKRR RDSEGEVLRK 

      1090       1100       1110       1120       1130       1140 
TAHRVDFIAK YLGGLREQEK RIKCLESQIN YCSVLVSSVA DVLAQGGGPR SSQHCGEGSQ 

      1150       1160 
LVAADHRGGL DGWEQPGAGQ PPSDT 

« Hide

Isoform 2 [UniParc].

Checksum: 868C5AB7095124A1
Show »

FASTA1,167131,688
Isoform 3 [UniParc].

Checksum: 6C2D1E4D16392D00
Show »

FASTA87297,738

References

« Hide 'large scale' references
[1]"Identification and characterization of MTR1, a novel gene with homology to melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical region on chromosome 11p15.5 and showing allele-specific expression."
Prawitt D., Enklaar T., Klemm G., Gaertner B., Spangenberg C., Winterpacht A., Higgins M., Pelletier J., Zabel B.
Hum. Mol. Genet. 9:203-216(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3), TISSUE SPECIFICITY.
[2]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-254.
[4]"Comparative sequence analysis and characterization of the imprinting cluster on the human chromosome 11p15.5 and distal mouse chromosome 7."
Paulsen M., El-Maarri O., Engemann S., Franck O., Stroedicke M., Davies K.R., Bowden L.M., Reinhardt R., Reik W., Harteneck C., Walter J.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-1165 (ISOFORM 2).
[5]"TRPM5 is a transient Ca2+-activated cation channel responding to rapid changes in [Ca2+]i."
Prawitt D., Monteilh-Zoller M.K., Brixel L., Spangenberg C., Zabel B., Fleig A., Penner R.
Proc. Natl. Acad. Sci. U.S.A. 100:15166-15171(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF177473 mRNA. Translation: AAF26288.1.
AC124057 Genomic DNA. No translation available.
BC093787 mRNA. Translation: AAH93787.1.
BC093789 mRNA. Translation: AAH93789.1.
AJ270996 mRNA. Translation: CAB66342.1.
CCDSCCDS31340.1. [Q9NZQ8-1]
RefSeqNP_055370.1. NM_014555.3. [Q9NZQ8-1]
UniGeneHs.272287.

3D structure databases

ProteinModelPortalQ9NZQ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000155858.

Chemistry

ChEMBLCHEMBL1628468.
GuidetoPHARMACOLOGY497.

Protein family/group databases

TCDB1.A.4.5.3. the transient receptor potential ca(2+) channel (trp-cc) family.

PTM databases

PhosphoSiteQ9NZQ8.

Polymorphism databases

DMDM74753086.

Proteomic databases

PaxDbQ9NZQ8.
PRIDEQ9NZQ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000155858; ENSP00000155858; ENSG00000070985. [Q9NZQ8-1]
ENST00000452833; ENSP00000387965; ENSG00000070985. [Q9NZQ8-2]
ENST00000533881; ENSP00000434383; ENSG00000070985.
GeneID29850.
KEGGhsa:29850.
UCSCuc001lwm.4. human. [Q9NZQ8-1]
uc009ydn.3. human. [Q9NZQ8-2]

Organism-specific databases

CTD29850.
GeneCardsGC11M002425.
HGNCHGNC:14323. TRPM5.
HPAHPA042115.
HPA043315.
MIM604600. gene.
neXtProtNX_Q9NZQ8.
PharmGKBPA37869.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG253824.
HOVERGENHBG108337.
InParanoidQ9NZQ8.
KOK04980.
OrthoDBEOG725DH1.
PhylomeDBQ9NZQ8.
TreeFamTF314204.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ9NZQ8.
BgeeQ9NZQ8.
CleanExHS_TRPM5.
GenevestigatorQ9NZQ8.

Family and domain databases

ProtoNetSearch...

Other

GeneWikiTRPM5.
GenomeRNAi29850.
NextBio52387.
PROQ9NZQ8.
SOURCESearch...

Entry information

Entry nameTRPM5_HUMAN
AccessionPrimary (citable) accession number: Q9NZQ8
Secondary accession number(s): A6NHS0, Q52LU2, Q9NY34
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM