Transient receptor potential cation channel subfamily M member 5

Names and origin

Protein names

Recommended name:
    Transient receptor potential cation channel subfamily M member 5
Alternative name(s):
    Long transient receptor potential channel 5
      Short name=LTrpC5
    MLSN1- and TRP-related gene 1 protein

Gene names

Name:	TRPM5
Synonyms:	LTRPC5, MTR1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	1165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Voltage-modulated Ca²⁺-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na⁺, K⁺ and Cs⁺ ions equally well. Activated directly by increases in intracellular Ca²⁺, but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca²⁺. Also activated by a fast intracellular Ca²⁺ increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. Ref.5 UniProtKB Q9JJH7
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Tissue specificity	Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression. Ref.1
Sequence similarities	Belongs to the transient receptor family. LTrpC subfamily. Ref.5

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Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane
Molecular function	Ionic channel Receptor Voltage-gated channel
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Ref.1 Traceable author statement. Source: ProtInc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	receptor activity Inferred from electronic annotation. Source: UniProtKB-KW voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.3 Ref.2 (identifier: Q9NZQ8-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.4 (identifier: Q9NZQ8-2) The sequence of this isoform differs from the canonical sequence as follows: 155-155: Q → QVH

Isoform 3 Ref.1 (identifier: Q9NZQ8-3) The sequence of this isoform differs from the canonical sequence as follows: 870-872: MKD → KPV 873-1165: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1165

1165

Transient receptor potential cation channel subfamily M member 5

PRO_0000328933

Regions

Topological domain

1 – 646

646

Cytoplasmic Potential

Transmembrane

647 – 667

21

Potential

Topological domain

668 – 733

66

Extracellular Potential

Transmembrane

734 – 754

21

Potential

Topological domain

755 – 811

57

Cytoplasmic Potential

Transmembrane

812 – 832

21

Potential

Topological domain

833 – 835

3

Extracellular Potential

Transmembrane

836 – 856

21

Potential

Topological domain

857 – 872

16

Cytoplasmic Potential

Transmembrane

873 – 893

21

Potential

Topological domain

894 – 954

61

Extracellular Potential

Transmembrane

955 – 975

21

Potential

Topological domain

976 – 1165

190

Cytoplasmic Potential

Coiled coil

553 – 581

29

Potential

Amino acid modifications

Modified residue

419

1

Phosphotyrosine Ref.6

Natural variations

Alternative sequence

155

1

Q → QVH in isoform 2. Ref.4

VSP_052741

Alternative sequence

870 – 872

3

MKD → KPV in isoform 3. Ref.1

VSP_052742

Alternative sequence

873 – 1165

293

Missing in isoform 3. Ref.1

VSP_052743

Natural variant

235

1

N → S: dbSNP rs886277.

VAR_052377

Natural variant

254

1

V → A: dbSNP rs3986599. Ref.3

VAR_042578

Natural variant

335

1

V → L: dbSNP rs34350821.

VAR_052378

Natural variant

456

1

A → T: dbSNP rs34551253.

VAR_052379

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C4AD5BAA866BE73B Show »	FASTA	1,165	131,451
10 20 30 40 50 60 MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW 70 80 90 100 110 120 HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV 130 140 150 160 170 180 RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQEDFPV HYPEDDGGSQ GPLCSLDSNL 190 200 210 220 230 240 SHFILVEPGP PGKGDGLTEL RLRLEKHISE QRAGYGGTGS IEIPVLCLLV NGDPNTLERI 250 260 270 280 290 300 SRAVEQAAPW LILVGSGGIA DVLAALVNQP HLLVPKVAEK QFKEKFPSKH FSWEDIVRWT 310 320 330 340 350 360 KLLQNITSHQ HLLTVYDFEQ EGSEELDTVI LKALVKACKS HSQEPQDYLD ELKLAVAWDR 370 380 390 400 410 420 VDIAKSEIFN GDVEWKSCDL EEVMVDALVS NKPEFVRLFV DNGADVADFL TYGRLQELYR 430 440 450 460 470 480 SVSRKSLLFD LLQRKQEEAR LTLAGLGTQQ AREPPAGPPA FSLHEVSRVL KDFLQDACRG 490 500 510 520 530 540 FYQDGRPGDR RRAEKGPAKR PTGQKWLLDL NQKSENPWRD LFLWAVLQNR HEMATYFWAM 550 560 570 580 590 600 GQEGVAAALA ACKILKEMSH LETEAEAARA TREAKYERLA LDLFSECYSN SEARAFALLV 610 620 630 640 650 660 RRNRCWSKTT CLHLATEADA KAFFAHDGVQ AFLTRIWWGD MAAGTPILRL LGAFLCPALV 670 680 690 700 710 720 YTNLITFSEE APLRTGLEDL QDLDSLDTEK SPLYGLQSRV EELVEAPRAQ GDRGPRAVFL 730 740 750 760 770 780 LTRWRKFWGA PVTVFLGNVV MYFAFLFLFT YVLLVDFRPP PQGPSGPEVT LYFWVFTLVL 790 800 810 820 830 840 EEIRQGFFTD EDTHLVKKFT LYVGDNWNKC DMVAIFLFIV GVTCRMLPSA FEAGRTVLAM 850 860 870 880 890 900 DFMVFTLRLI HIFAIHKQLG PKIIVVERMM KDVFFFLFFL SVWLVAYGVT TQALLHPHDG 910 920 930 940 950 960 RLEWIFRRVL YRPYLQIFGQ IPLDEIDEAR VNCSTHPLLL EDSPSCPSLY ANWLVILLLV 970 980 990 1000 1010 1020 TFLLVTNVLL MNLLIAMFSY TFQVVQGNAD MFWKFQRYNL IVEYHERPAL APPFILLSHL 1030 1040 1050 1060 1070 1080 SLTLRRVFKK EAEHKREHLE RDLPDPLDQK VVTWETVQKE NFLSKMEKRR RDSEGEVLRK 1090 1100 1110 1120 1130 1140 TAHRVDFIAK YLGGLREQEK RIKCLESQIN YCSVLVSSVA DVLAQGGGPR SSQHCGEGSQ 1150 1160 LVAADHRGGL DGWEQPGAGQ PPSDT « Hide
Isoform 2. Checksum: 868C5AB7095124A1 Show »	FASTA	1,167	131,688
10 20 30 40 50 60 MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW 70 80 90 100 110 120 HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV 130 140 150 160 170 180 RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQVHEDF PVHYPEDDGG SQGPLCSLDS 190 200 210 220 230 240 NLSHFILVEP GPPGKGDGLT ELRLRLEKHI SEQRAGYGGT GSIEIPVLCL LVNGDPNTLE 250 260 270 280 290 300 RISRAVEQAA PWLILVGSGG IADVLAALVN QPHLLVPKVA EKQFKEKFPS KHFSWEDIVR 310 320 330 340 350 360 WTKLLQNITS HQHLLTVYDF EQEGSEELDT VILKALVKAC KSHSQEPQDY LDELKLAVAW 370 380 390 400 410 420 DRVDIAKSEI FNGDVEWKSC DLEEVMVDAL VSNKPEFVRL FVDNGADVAD FLTYGRLQEL 430 440 450 460 470 480 YRSVSRKSLL FDLLQRKQEE ARLTLAGLGT QQAREPPAGP PAFSLHEVSR VLKDFLQDAC 490 500 510 520 530 540 RGFYQDGRPG DRRRAEKGPA KRPTGQKWLL DLNQKSENPW RDLFLWAVLQ NRHEMATYFW 550 560 570 580 590 600 AMGQEGVAAA LAACKILKEM SHLETEAEAA RATREAKYER LALDLFSECY SNSEARAFAL 610 620 630 640 650 660 LVRRNRCWSK TTCLHLATEA DAKAFFAHDG VQAFLTRIWW GDMAAGTPIL RLLGAFLCPA 670 680 690 700 710 720 LVYTNLITFS EEAPLRTGLE DLQDLDSLDT EKSPLYGLQS RVEELVEAPR AQGDRGPRAV 730 740 750 760 770 780 FLLTRWRKFW GAPVTVFLGN VVMYFAFLFL FTYVLLVDFR PPPQGPSGPE VTLYFWVFTL 790 800 810 820 830 840 VLEEIRQGFF TDEDTHLVKK FTLYVGDNWN KCDMVAIFLF IVGVTCRMLP SAFEAGRTVL 850 860 870 880 890 900 AMDFMVFTLR LIHIFAIHKQ LGPKIIVVER MMKDVFFFLF FLSVWLVAYG VTTQALLHPH 910 920 930 940 950 960 DGRLEWIFRR VLYRPYLQIF GQIPLDEIDE ARVNCSTHPL LLEDSPSCPS LYANWLVILL 970 980 990 1000 1010 1020 LVTFLLVTNV LLMNLLIAMF SYTFQVVQGN ADMFWKFQRY NLIVEYHERP ALAPPFILLS 1030 1040 1050 1060 1070 1080 HLSLTLRRVF KKEAEHKREH LERDLPDPLD QKVVTWETVQ KENFLSKMEK RRRDSEGEVL 1090 1100 1110 1120 1130 1140 RKTAHRVDFI AKYLGGLREQ EKRIKCLESQ INYCSVLVSS VADVLAQGGG PRSSQHCGEG 1150 1160 SQLVAADHRG GLDGWEQPGA GQPPSDT « Hide
Isoform 3. Checksum: 6C2D1E4D16392D00 Show »	FASTA	872	97,738
10 20 30 40 50 60 MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW 70 80 90 100 110 120 HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV 130 140 150 160 170 180 RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQEDFPV HYPEDDGGSQ GPLCSLDSNL 190 200 210 220 230 240 SHFILVEPGP PGKGDGLTEL RLRLEKHISE QRAGYGGTGS IEIPVLCLLV NGDPNTLERI 250 260 270 280 290 300 SRAVEQAAPW LILVGSGGIA DVLAALVNQP HLLVPKVAEK QFKEKFPSKH FSWEDIVRWT 310 320 330 340 350 360 KLLQNITSHQ HLLTVYDFEQ EGSEELDTVI LKALVKACKS HSQEPQDYLD ELKLAVAWDR 370 380 390 400 410 420 VDIAKSEIFN GDVEWKSCDL EEVMVDALVS NKPEFVRLFV DNGADVADFL TYGRLQELYR 430 440 450 460 470 480 SVSRKSLLFD LLQRKQEEAR LTLAGLGTQQ AREPPAGPPA FSLHEVSRVL KDFLQDACRG 490 500 510 520 530 540 FYQDGRPGDR RRAEKGPAKR PTGQKWLLDL NQKSENPWRD LFLWAVLQNR HEMATYFWAM 550 560 570 580 590 600 GQEGVAAALA ACKILKEMSH LETEAEAARA TREAKYERLA LDLFSECYSN SEARAFALLV 610 620 630 640 650 660 RRNRCWSKTT CLHLATEADA KAFFAHDGVQ AFLTRIWWGD MAAGTPILRL LGAFLCPALV 670 680 690 700 710 720 YTNLITFSEE APLRTGLEDL QDLDSLDTEK SPLYGLQSRV EELVEAPRAQ GDRGPRAVFL 730 740 750 760 770 780 LTRWRKFWGA PVTVFLGNVV MYFAFLFLFT YVLLVDFRPP PQGPSGPEVT LYFWVFTLVL 790 800 810 820 830 840 EEIRQGFFTD EDTHLVKKFT LYVGDNWNKC DMVAIFLFIV GVTCRMLPSA FEAGRTVLAM 850 860 870 DFMVFTLRLI HIFAIHKQLG PKIIVVERMK PV « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of MTR1, a novel gene with homology to melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical region on chromosome 11p15.5 and showing allele-specific expression." Prawitt D., Enklaar T., Klemm G., Gaertner B., Spangenberg C., Winterpacht A., Higgins M., Pelletier J., Zabel B. Hum. Mol. Genet. 9:203-216(2000) [PubMed: 10607831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3), TISSUE SPECIFICITY.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-254.
[4]	"Comparative sequence analysis and characterization of the imprinting cluster on the human chromosome 11p15.5 and distal mouse chromosome 7." Paulsen M., El-Maarri O., Engemann S., Franck O., Stroedicke M., Davies K.R., Bowden L.M., Reinhardt R., Reik W., Harteneck C., Walter J. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-1165 (ISOFORM 2).
[5]	"TRPM5 is a transient Ca2+-activated cation channel responding to rapid changes in [Ca2+]i." Prawitt D., Monteilh-Zoller M.K., Brixel L., Spangenberg C., Zabel B., Fleig A., Penner R. Proc. Natl. Acad. Sci. U.S.A. 100:15166-15171(2003) [PubMed: 14634208] [Abstract] Cited for: FUNCTION.
[6]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-419, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF177473 mRNA. Translation: AAF26288.1. AC124057 Genomic DNA. No translation available. BC093787 mRNA. Translation: AAH93787.1. BC093789 mRNA. Translation: AAH93789.1. AJ270996 mRNA. Translation: CAB66342.1.
IPI	IPI00329503. IPI00889724. IPI00889744.
RefSeq	NP_055370.1.
UniGene	Hs.272287
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	1.A.4.5.3. transient receptor potential Ca2+ channel (TRP-CC) family.
Proteomic databases
PRIDE	Q9NZQ8.
Genome annotation databases
Ensembl	ENSG00000070985. Homo sapiens. [Contig view]
GeneID	29850.
KEGG	hsa:29850.
Organism-specific databases
GeneCards	GC11M002382.
HGNC	HGNC:14323. TRPM5.
MIM	604600. gene.
PharmGKB	PA37869.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q9NZQ8.
OMA	Q9NZQ8. LVKACKS.
Gene expression databases
ArrayExpress	Q9NZQ8.
Bgee	Q9NZQ8.
CleanEx	HS_TRPM5.
Family and domain databases
InterPro	IPR005821. Ion_trans. [Graphical view]
Pfam	PF00520. Ion_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	52387.
SOURCE	Search...

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Entry information

Entry name

TRPM5_HUMAN

Accession

Primary (citable) accession number: Q9NZQ8
Secondary accession number(s): A6NHS0, Q52LU2, Q9NY34

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents