Q9NZQ8 (TRPM5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transient receptor potential cation channel subfamily M member 5

Alternative name(s):
Long transient receptor potential channel 5
Short name=LTrpC-5
Short name=LTrpC5
MLSN1- and TRP-related gene 1 protein

Gene names

Name:	TRPM5
Synonyms:	LTRPC5, MTR1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1165 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Voltage-modulated Ca2+-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na+, K+ and Cs+ ions equally well. Activated directly by increases in intracellular Ca2+, but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca2+. Also activated by a fast intracellular Ca2+ increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. Ref.5 UniProtKB Q9JJH7
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Tissue specificity	Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression. Ref.1
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM5 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Traceable author statement Ref.1. Source: ProtInc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium activated cation channel activity Inferred from electronic annotation. Source: Compara ion channel activity Traceable author statement Ref.1. Source: ProtInc potassium channel activity Inferred from electronic annotation. Source: Compara sodium channel activity Inferred from electronic annotation. Source: Compara voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.2 Ref.3 (identifier: Q9NZQ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.4 (identifier: Q9NZQ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     155-155: Q → QVH

Isoform 3 Ref.1 (identifier: Q9NZQ8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     870-872: MKD → KPV
     873-1165: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1165	1165	Transient receptor potential cation channel subfamily M member 5		PRO_0000328933
Regions
	Topological domain	1 – 646	646	Cytoplasmic Potential
	Transmembrane	647 – 667	21	Helical; Potential
	Topological domain	668 – 733	66	Extracellular Potential
	Transmembrane	734 – 754	21	Helical; Potential
	Topological domain	755 – 811	57	Cytoplasmic Potential
	Transmembrane	812 – 832	21	Helical; Potential
	Topological domain	833 – 835	3	Extracellular Potential
	Transmembrane	836 – 856	21	Helical; Potential
	Topological domain	857 – 872	16	Cytoplasmic Potential
	Transmembrane	873 – 893	21	Helical; Potential
	Topological domain	894 – 954	61	Extracellular Potential
	Transmembrane	955 – 975	21	Helical; Potential
	Topological domain	976 – 1165	190	Cytoplasmic Potential
	Coiled coil	553 – 581	29	Potential
Natural variations
	Alternative sequence	155	1	Q → QVH in isoform 2. Ref.4		VSP_052741
	Alternative sequence	870 – 872	3	MKD → KPV in isoform 3. Ref.1		VSP_052742
	Alternative sequence	873 – 1165	293	Missing in isoform 3. Ref.1		VSP_052743
	Natural variant	235	1	N → S. Corresponds to variant rs886277 [ dbSNP | Ensembl ].		VAR_052377
	Natural variant	254	1	V → A. Ref.3 Corresponds to variant rs3986599 [ dbSNP | Ensembl ].		VAR_042578
	Natural variant	335	1	V → L. Corresponds to variant rs34350821 [ dbSNP | Ensembl ].		VAR_052378
	Natural variant	456	1	A → T. Corresponds to variant rs34551253 [ dbSNP | Ensembl ].		VAR_052379
	Natural variant	578	1	R → Q. Corresponds to variant rs4929982 [ dbSNP | Ensembl ].		VAR_059836

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C4AD5BAA866BE73B	FASTA	1,165	131,451
	10 20 30 40 50 60 MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW 70 80 90 100 110 120 HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV 130 140 150 160 170 180 RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQEDFPV HYPEDDGGSQ GPLCSLDSNL 190 200 210 220 230 240 SHFILVEPGP PGKGDGLTEL RLRLEKHISE QRAGYGGTGS IEIPVLCLLV NGDPNTLERI 250 260 270 280 290 300 SRAVEQAAPW LILVGSGGIA DVLAALVNQP HLLVPKVAEK QFKEKFPSKH FSWEDIVRWT 310 320 330 340 350 360 KLLQNITSHQ HLLTVYDFEQ EGSEELDTVI LKALVKACKS HSQEPQDYLD ELKLAVAWDR 370 380 390 400 410 420 VDIAKSEIFN GDVEWKSCDL EEVMVDALVS NKPEFVRLFV DNGADVADFL TYGRLQELYR 430 440 450 460 470 480 SVSRKSLLFD LLQRKQEEAR LTLAGLGTQQ AREPPAGPPA FSLHEVSRVL KDFLQDACRG 490 500 510 520 530 540 FYQDGRPGDR RRAEKGPAKR PTGQKWLLDL NQKSENPWRD LFLWAVLQNR HEMATYFWAM 550 560 570 580 590 600 GQEGVAAALA ACKILKEMSH LETEAEAARA TREAKYERLA LDLFSECYSN SEARAFALLV 610 620 630 640 650 660 RRNRCWSKTT CLHLATEADA KAFFAHDGVQ AFLTRIWWGD MAAGTPILRL LGAFLCPALV 670 680 690 700 710 720 YTNLITFSEE APLRTGLEDL QDLDSLDTEK SPLYGLQSRV EELVEAPRAQ GDRGPRAVFL 730 740 750 760 770 780 LTRWRKFWGA PVTVFLGNVV MYFAFLFLFT YVLLVDFRPP PQGPSGPEVT LYFWVFTLVL 790 800 810 820 830 840 EEIRQGFFTD EDTHLVKKFT LYVGDNWNKC DMVAIFLFIV GVTCRMLPSA FEAGRTVLAM 850 860 870 880 890 900 DFMVFTLRLI HIFAIHKQLG PKIIVVERMM KDVFFFLFFL SVWLVAYGVT TQALLHPHDG 910 920 930 940 950 960 RLEWIFRRVL YRPYLQIFGQ IPLDEIDEAR VNCSTHPLLL EDSPSCPSLY ANWLVILLLV 970 980 990 1000 1010 1020 TFLLVTNVLL MNLLIAMFSY TFQVVQGNAD MFWKFQRYNL IVEYHERPAL APPFILLSHL 1030 1040 1050 1060 1070 1080 SLTLRRVFKK EAEHKREHLE RDLPDPLDQK VVTWETVQKE NFLSKMEKRR RDSEGEVLRK 1090 1100 1110 1120 1130 1140 TAHRVDFIAK YLGGLREQEK RIKCLESQIN YCSVLVSSVA DVLAQGGGPR SSQHCGEGSQ 1150 1160 LVAADHRGGL DGWEQPGAGQ PPSDT
	Isoform 2 [UniParc]. Checksum: 868C5AB7095124A1	FASTA	1,167	131,688
	10 20 30 40 50 60 MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW 70 80 90 100 110 120 HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV 130 140 150 160 170 180 RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQVHEDF PVHYPEDDGG SQGPLCSLDS 190 200 210 220 230 240 NLSHFILVEP GPPGKGDGLT ELRLRLEKHI SEQRAGYGGT GSIEIPVLCL LVNGDPNTLE 250 260 270 280 290 300 RISRAVEQAA PWLILVGSGG IADVLAALVN QPHLLVPKVA EKQFKEKFPS KHFSWEDIVR 310 320 330 340 350 360 WTKLLQNITS HQHLLTVYDF EQEGSEELDT VILKALVKAC KSHSQEPQDY LDELKLAVAW 370 380 390 400 410 420 DRVDIAKSEI FNGDVEWKSC DLEEVMVDAL VSNKPEFVRL FVDNGADVAD FLTYGRLQEL 430 440 450 460 470 480 YRSVSRKSLL FDLLQRKQEE ARLTLAGLGT QQAREPPAGP PAFSLHEVSR VLKDFLQDAC 490 500 510 520 530 540 RGFYQDGRPG DRRRAEKGPA KRPTGQKWLL DLNQKSENPW RDLFLWAVLQ NRHEMATYFW 550 560 570 580 590 600 AMGQEGVAAA LAACKILKEM SHLETEAEAA RATREAKYER LALDLFSECY SNSEARAFAL 610 620 630 640 650 660 LVRRNRCWSK TTCLHLATEA DAKAFFAHDG VQAFLTRIWW GDMAAGTPIL RLLGAFLCPA 670 680 690 700 710 720 LVYTNLITFS EEAPLRTGLE DLQDLDSLDT EKSPLYGLQS RVEELVEAPR AQGDRGPRAV 730 740 750 760 770 780 FLLTRWRKFW GAPVTVFLGN VVMYFAFLFL FTYVLLVDFR PPPQGPSGPE VTLYFWVFTL 790 800 810 820 830 840 VLEEIRQGFF TDEDTHLVKK FTLYVGDNWN KCDMVAIFLF IVGVTCRMLP SAFEAGRTVL 850 860 870 880 890 900 AMDFMVFTLR LIHIFAIHKQ LGPKIIVVER MMKDVFFFLF FLSVWLVAYG VTTQALLHPH 910 920 930 940 950 960 DGRLEWIFRR VLYRPYLQIF GQIPLDEIDE ARVNCSTHPL LLEDSPSCPS LYANWLVILL 970 980 990 1000 1010 1020 LVTFLLVTNV LLMNLLIAMF SYTFQVVQGN ADMFWKFQRY NLIVEYHERP ALAPPFILLS 1030 1040 1050 1060 1070 1080 HLSLTLRRVF KKEAEHKREH LERDLPDPLD QKVVTWETVQ KENFLSKMEK RRRDSEGEVL 1090 1100 1110 1120 1130 1140 RKTAHRVDFI AKYLGGLREQ EKRIKCLESQ INYCSVLVSS VADVLAQGGG PRSSQHCGEG 1150 1160 SQLVAADHRG GLDGWEQPGA GQPPSDT
	Isoform 3 [UniParc]. Checksum: 6C2D1E4D16392D00	FASTA	872	97,738
	10 20 30 40 50 60 MQDVQGPRPG SPGDAEDRRE LGLHRGEVNF GGSGKKRGKF VRVPSGVAPS VLFDLLLAEW 70 80 90 100 110 120 HLPAPNLVVS LVGEEQPFAM KSWLRDVLRK GLVKAAQSTG AWILTSALRV GLARHVGQAV 130 140 150 160 170 180 RDHSLASTST KVRVVAVGMA SLGRVLHRRI LEEAQEDFPV HYPEDDGGSQ GPLCSLDSNL 190 200 210 220 230 240 SHFILVEPGP PGKGDGLTEL RLRLEKHISE QRAGYGGTGS IEIPVLCLLV NGDPNTLERI 250 260 270 280 290 300 SRAVEQAAPW LILVGSGGIA DVLAALVNQP HLLVPKVAEK QFKEKFPSKH FSWEDIVRWT 310 320 330 340 350 360 KLLQNITSHQ HLLTVYDFEQ EGSEELDTVI LKALVKACKS HSQEPQDYLD ELKLAVAWDR 370 380 390 400 410 420 VDIAKSEIFN GDVEWKSCDL EEVMVDALVS NKPEFVRLFV DNGADVADFL TYGRLQELYR 430 440 450 460 470 480 SVSRKSLLFD LLQRKQEEAR LTLAGLGTQQ AREPPAGPPA FSLHEVSRVL KDFLQDACRG 490 500 510 520 530 540 FYQDGRPGDR RRAEKGPAKR PTGQKWLLDL NQKSENPWRD LFLWAVLQNR HEMATYFWAM 550 560 570 580 590 600 GQEGVAAALA ACKILKEMSH LETEAEAARA TREAKYERLA LDLFSECYSN SEARAFALLV 610 620 630 640 650 660 RRNRCWSKTT CLHLATEADA KAFFAHDGVQ AFLTRIWWGD MAAGTPILRL LGAFLCPALV 670 680 690 700 710 720 YTNLITFSEE APLRTGLEDL QDLDSLDTEK SPLYGLQSRV EELVEAPRAQ GDRGPRAVFL 730 740 750 760 770 780 LTRWRKFWGA PVTVFLGNVV MYFAFLFLFT YVLLVDFRPP PQGPSGPEVT LYFWVFTLVL 790 800 810 820 830 840 EEIRQGFFTD EDTHLVKKFT LYVGDNWNKC DMVAIFLFIV GVTCRMLPSA FEAGRTVLAM 850 860 870 DFMVFTLRLI HIFAIHKQLG PKIIVVERMK PV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of MTR1, a novel gene with homology to melastatin (MLSN1) and the trp gene family located in the BWS-WT2 critical region on chromosome 11p15.5 and showing allele-specific expression." Prawitt D., Enklaar T., Klemm G., Gaertner B., Spangenberg C., Winterpacht A., Higgins M., Pelletier J., Zabel B. Hum. Mol. Genet. 9:203-216(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORM 3), TISSUE SPECIFICITY.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-254.
[4]	"Comparative sequence analysis and characterization of the imprinting cluster on the human chromosome 11p15.5 and distal mouse chromosome 7." Paulsen M., El-Maarri O., Engemann S., Franck O., Stroedicke M., Davies K.R., Bowden L.M., Reinhardt R., Reik W., Harteneck C., Walter J. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-1165 (ISOFORM 2).
[5]	"TRPM5 is a transient Ca2+-activated cation channel responding to rapid changes in [Ca2+]i." Prawitt D., Monteilh-Zoller M.K., Brixel L., Spangenberg C., Zabel B., Fleig A., Penner R. Proc. Natl. Acad. Sci. U.S.A. 100:15166-15171(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177473 mRNA. Translation: AAF26288.1. AC124057 Genomic DNA. No translation available. BC093787 mRNA. Translation: AAH93787.1. BC093789 mRNA. Translation: AAH93789.1. AJ270996 mRNA. Translation: CAB66342.1.
IPI	IPI00329503. IPI00889744. IPI00942704.
RefSeq	NP_055370.1. NM_014555.3.
UniGene	Hs.272287.
3D structure databases
ProteinModelPortal	Q9NZQ8.
SMR	Q9NZQ8. Positions 1079-1124.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000155858.
Protein family/group databases
TCDB	1.A.4.5.3. transient receptor potential Ca2+ channel (TRP-CC) family.
PTM databases
PhosphoSite	Q9NZQ8.
Polymorphism databases
DMDM	74753086.
Proteomic databases
PaxDb	Q9NZQ8.
PRIDE	Q9NZQ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000155858; ENSP00000155858; ENSG00000070985. ENST00000452833; ENSP00000387965; ENSG00000070985. ENST00000533881; ENSP00000434383; ENSG00000070985.
GeneID	29850.
KEGG	hsa:29850.
UCSC	uc001lwm.4. human. uc009ydn.3. human.
Organism-specific databases
CTD	29850.
GeneCards	GC11M002425.
HGNC	HGNC:14323. TRPM5.
HPA	HPA042115. HPA043315.
MIM	604600. gene.
neXtProt	NX_Q9NZQ8.
PharmGKB	PA37869.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG253824.
HOVERGEN	HBG108337.
InParanoid	Q9NZQ8.
KO	K04980.
OrthoDB	EOG47D9FD.
Gene expression databases
ArrayExpress	Q9NZQ8.
Bgee	Q9NZQ8.
CleanEx	HS_TRPM5.
Genevestigator	Q9NZQ8.
Family and domain databases
ProtoNet	Search...
Other
ChEMBL	CHEMBL1628468.
GenomeRNAi	29850.
NextBio	52387.
SOURCE	Search...

Entry information

Entry name

TRPM5_HUMAN

Accession

Primary (citable) accession number: Q9NZQ8
Secondary accession number(s): A6NHS0, Q52LU2, Q9NY34

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.