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Q9NZQ7 (PD1L1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Programmed cell death 1 ligand 1

Short name=PD-L1
Short name=PDCD1 ligand 1
Short name=Programmed death ligand 1
Alternative name(s):
B7 homolog 1
Short name=B7-H1
CD_antigen=CD274
Gene names
Name:CD274
Synonyms:B7H1, PDCD1L1, PDCD1LG1, PDL1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Ref.1 Ref.2

Subunit structure

Interacts with PDCD1. Ref.2

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein Ref.3.

Isoform 2: Endomembrane system; Single-pass type I membrane protein Ref.3.

Tissue specificity

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. Ref.1 Ref.2

Induction

Up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. Up-regulated in B-cells activated by surface Ig cross-linking. Ref.1 Ref.2

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution

The sequence BAA91966.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NZQ7-1)

Also known as: PD-L1I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NZQ7-2)

Also known as: PD-L1II;

The sequence of this isoform differs from the canonical sequence as follows:
     19-132: Missing.
Isoform 3 (identifier: Q9NZQ7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     178-178: K → D
     179-290: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 290272Programmed cell death 1 ligand 1
PRO_0000014553

Regions

Topological domain19 – 238220Extracellular Potential
Transmembrane239 – 25921Helical; Potential
Topological domain260 – 29031Cytoplasmic Potential
Domain19 – 127109Ig-like V-type
Domain133 – 22593Ig-like C2-type

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation1921N-linked (GlcNAc...) Ref.9
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2191N-linked (GlcNAc...) Potential
Disulfide bond40 ↔ 114 Ref.10
Disulfide bond155 ↔ 209 Ref.10

Natural variations

Alternative sequence19 – 132114Missing in isoform 2.
VSP_013735
Alternative sequence1781K → D in isoform 3.
VSP_013736
Alternative sequence179 – 290112Missing in isoform 3.
VSP_013737

Secondary structure

........................................ 290
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PD-L1I) [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: FE957086E62A31A8

FASTA29033,275
        10         20         30         40         50         60 
MRIFAVFIFM TYWHLLNAFT VTVPKDLYVV EYGSNMTIEC KFPVEKQLDL AALIVYWEME 

        70         80         90        100        110        120 
DKNIIQFVHG EEDLKVQHSS YRQRARLLKD QLSLGNAALQ ITDVKLQDAG VYRCMISYGG 

       130        140        150        160        170        180 
ADYKRITVKV NAPYNKINQR ILVVDPVTSE HELTCQAEGY PKAEVIWTSS DHQVLSGKTT 

       190        200        210        220        230        240 
TTNSKREEKL FNVTSTLRIN TTTNEIFYCT FRRLDPEENH TAELVIPELP LAHPPNERTH 

       250        260        270        280        290 
LVILGAILLC LGVALTFIFR LRKGRMMDVK KCGIQDTNSK KQSDTHLEET 

« Hide

Isoform 2 (PD-L1II) [UniParc].

Checksum: B8BD0CFF9DEA722F
Show »

FASTA17620,246
Isoform 3 [UniParc].

Checksum: A306BF2018885F0B
Show »

FASTA17820,454

References

« Hide 'large scale' references
[1]"B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."
Dong H., Zhu G., Tamada K., Chen L.
Nat. Med. 5:1365-1369(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[2]"Engagement of the PD-1 immunoinhibitory receptor by a novel B7-family member leads to negative regulation of lymphocyte activation."
Freeman G.J., Long A.J., Iwai Y., Bourque K., Chernova T., Nishimura H., Fitz L.J., Malenkovich N., Okazaki T., Byrne M.C., Horton H.F., Fouser L., Carter L., Ling V., Bowman M.R., Carreno B.M., Collins M., Wood C.R., Honjo T.
J. Exp. Med. 192:1027-1034(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH PDCD1, TISSUE SPECIFICITY, INDUCTION.
Tissue: Placenta.
[3]"Identification of a novel splice variant of human PD-L1 mRNA encoding an isoform-lacking Igv-like domain."
He X.-H., Xu L.-H., Liu Y.
Acta Pharmacol. Sin. 26:462-468(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION.
[4]"Expression and significance of PD-L1 variant within tissues of human gastric carcinoma."
Chi X.-Y., Zha Q.-B., Xu L.-H., Jia Q.-T., Li F.-Y., He X.-H.
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Gastric carcinoma.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: Placenta.
[6]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[9]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-192.
Tissue: Liver.
[10]"The PD-1/PD-L1 complex resembles the antigen-binding Fv domains of antibodies and T cell receptors."
Lin D.Y., Tanaka Y., Iwasaki M., Gittis A.G., Su H.P., Mikami B., Okazaki T., Honjo T., Minato N., Garboczi D.N.
Proc. Natl. Acad. Sci. U.S.A. 105:3011-3016(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.64 ANGSTROMS) OF 18-239 IN COMPLEX WITH MOUSE PDCD1, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF177937 mRNA. Translation: AAF25807.1.
AF233516 mRNA. Translation: AAG18508.1.
AY254342 mRNA. Translation: AAP13470.1.
AY291313 mRNA. Translation: AAP42144.1.
AY714881 mRNA. Translation: AAU09634.1.
DQ286582 mRNA. Translation: ABB90152.1.
AK001894 mRNA. Translation: BAA91966.1. Different initiation.
AK300470 mRNA. Translation: BAG62189.1.
AK314567 mRNA. Translation: BAG37149.1.
AL162253 Genomic DNA. Translation: CAI15983.1.
CH471071 Genomic DNA. Translation: EAW58763.1.
BC069381 mRNA. Translation: AAH69381.1.
BC074984 mRNA. Translation: AAH74984.1.
BC113734 mRNA. Translation: AAI13735.1.
BC113736 mRNA. Translation: AAI13737.1.
RefSeqNP_001254635.1. NM_001267706.1.
NP_054862.1. NM_014143.3.
UniGeneHs.521989.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3BIKX-ray2.65A18-239[»]
3BISX-ray2.64A/B18-239[»]
3FN3X-ray2.70A/B19-238[»]
3SBWX-ray2.28C19-239[»]
ProteinModelPortalQ9NZQ7.
SMRQ9NZQ7. Positions 19-230.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118891. 4 interactions.
DIPDIP-29731N.
IntActQ9NZQ7. 2 interactions.
STRING9606.ENSP00000370989.

PTM databases

PhosphoSiteQ9NZQ7.

Polymorphism databases

DMDM83287884.

Proteomic databases

PaxDbQ9NZQ7.
PRIDEQ9NZQ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000381573; ENSP00000370985; ENSG00000120217. [Q9NZQ7-2]
ENST00000381577; ENSP00000370989; ENSG00000120217. [Q9NZQ7-1]
GeneID29126.
KEGGhsa:29126.
UCSCuc003zje.3. human. [Q9NZQ7-1]
uc011lmb.2. human. [Q9NZQ7-3]

Organism-specific databases

CTD29126.
GeneCardsGC09P005444.
HGNCHGNC:17635. CD274.
HPACAB030018.
MIM605402. gene.
neXtProtNX_Q9NZQ7.
PharmGKBPA134915280.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43849.
HOGENOMHOG000059625.
HOVERGENHBG082112.
InParanoidQ9NZQ7.
KOK06745.
OMAAEVIWTS.
OrthoDBEOG7T7GTP.
PhylomeDBQ9NZQ7.
TreeFamTF331083.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ9NZQ7.
BgeeQ9NZQ7.
CleanExHS_CD274.
GenevestigatorQ9NZQ7.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9NZQ7.
GeneWikiPD-L1.
GenomeRNAi29126.
NextBio52236.
PROQ9NZQ7.
SOURCESearch...

Entry information

Entry namePD1L1_HUMAN
AccessionPrimary (citable) accession number: Q9NZQ7
Secondary accession number(s): B2RBA2 expand/collapse secondary AC list , B4DU27, Q14CJ2, Q2V8D5, Q66RK1, Q6WEX4, Q9NUZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries