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Protein

DnaJ homolog subfamily C member 27

Gene

DNAJC27

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 308GTPBy similarity
Nucleotide bindingi71 – 755GTPBy similarity
Nucleotide bindingi134 – 1374GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 27
Alternative name(s):
Rab and DnaJ domain-containing protein
Gene namesi
Name:DNAJC27
Synonyms:RABJS, RBJ
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:30290. DNAJC27.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Oncogene

Organism-specific databases

PharmGKBiPA164718860.

Polymorphism and mutation databases

BioMutaiDNAJC27.
DMDMi74734733.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273DnaJ homolog subfamily C member 27PRO_0000332975Add
BLAST

Proteomic databases

PaxDbiQ9NZQ0.
PeptideAtlasiQ9NZQ0.
PRIDEiQ9NZQ0.

PTM databases

iPTMnetiQ9NZQ0.
PhosphoSiteiQ9NZQ0.

Expressioni

Tissue specificityi

Overexpressed in gastrointestinal cancers; expression correlates with later tumor-node-metastasis stages of colorectal cancers.1 Publication

Gene expression databases

BgeeiQ9NZQ0.
CleanExiHS_DNAJC27.
GenevisibleiQ9NZQ0. HS.

Organism-specific databases

HPAiHPA036815.
HPA036816.

Interactioni

Subunit structurei

Interacts directly with MAPK1 (wild-type and kinase-deficient forms). Interacts directly (in GTP-bound form) with MAP2K1 (wild-type and kinase-deficient forms).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TFCP2Q128003EBI-10317544,EBI-717422

Protein-protein interaction databases

BioGridi119429. 1 interaction.
IntActiQ9NZQ0. 1 interaction.
STRINGi9606.ENSP00000264711.

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi203 – 21311Combined sources
Helixi218 – 2225Combined sources
Helixi230 – 24415Combined sources
Turni246 – 2483Combined sources
Helixi254 – 27219Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YS8NMR-A197-273[»]
ProteinModelPortaliQ9NZQ0.
SMRiQ9NZQ0. Positions 17-177, 201-273.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NZQ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini217 – 27357JPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1818Required for interaction with MAPK1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated
Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0098. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233969.
HOVERGENiHBG056504.
InParanoidiQ9NZQ0.
KOiK19372.
OMAiFYVSIVD.
OrthoDBiEOG7J70GQ.
PhylomeDBiQ9NZQ0.
TreeFamiTF328564.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEANMPKRKE PGRSLRIKVI SMGNAEVGKS CIIKRYCEKR FVSKYLATIG
60 70 80 90 100
IDYGVTKVHV RDREIKVNIF DMAGHPFFYE VRNEFYKDTQ GVILVYDVGQ
110 120 130 140 150
KDSFDALDAW LAEMKQELGP HGNMENIIFV VCANKIDCTK HRCVDESEGR
160 170 180 190 200
LWAESKGFLY FETSAQTGEG INEMFQTFYI SIVDLCENGG KRPTTNSSAS
210 220 230 240 250
FTKEQADAIR RIRNSKDSWD MLGVKPGASR DEVNKAYRKL AVLLHPDKCV
260 270
APGSEDAFKA VVNARTALLK NIK
Length:273
Mass (Da):30,855
Last modified:October 1, 2000 - v1
Checksum:iA3A0CE43EA6A2BCA
GO
Isoform 2 (identifier: Q9NZQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-90: VRNEFYKDTQ → MRKLRRREVK
     91-273: Missing.

Show »
Length:90
Mass (Da):10,622
Checksum:i24A41685FE2A3C94
GO
Isoform 3 (identifier: Q9NZQ0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     177-177: T → G
     178-273: Missing.

Show »
Length:177
Mass (Da):20,264
Checksum:iEECCC11FF0F5130B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei81 – 9010VRNEFYKDTQ → MRKLRRREVK in isoform 2. 1 PublicationVSP_033409
Alternative sequencei91 – 273183Missing in isoform 2. 1 PublicationVSP_033410Add
BLAST
Alternative sequencei177 – 1771T → G in isoform 3. 1 PublicationVSP_033411
Alternative sequencei178 – 27396Missing in isoform 3. 1 PublicationVSP_033412Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178983 mRNA. Translation: AAF44347.1.
AY094594 mRNA. Translation: AAM22521.1.
AL137731 mRNA. Translation: CAI46214.1.
CH471053 Genomic DNA. Translation: EAX00736.1.
CH471053 Genomic DNA. Translation: EAX00735.1.
CH471053 Genomic DNA. Translation: EAX00737.1.
BC034049 mRNA. Translation: AAH34049.1.
BK001284 mRNA. Translation: DAA01323.1.
CCDSiCCDS1716.1. [Q9NZQ0-1]
CCDS74493.1. [Q9NZQ0-3]
RefSeqiNP_001185488.1. NM_001198559.1. [Q9NZQ0-3]
NP_057628.1. NM_016544.2. [Q9NZQ0-1]
UniGeneiHs.434993.

Genome annotation databases

EnsembliENST00000264711; ENSP00000264711; ENSG00000115137. [Q9NZQ0-1]
ENST00000380809; ENSP00000370187; ENSG00000115137. [Q9NZQ0-2]
ENST00000534855; ENSP00000440086; ENSG00000115137. [Q9NZQ0-3]
GeneIDi51277.
KEGGihsa:51277.
UCSCiuc002rft.2. human. [Q9NZQ0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178983 mRNA. Translation: AAF44347.1.
AY094594 mRNA. Translation: AAM22521.1.
AL137731 mRNA. Translation: CAI46214.1.
CH471053 Genomic DNA. Translation: EAX00736.1.
CH471053 Genomic DNA. Translation: EAX00735.1.
CH471053 Genomic DNA. Translation: EAX00737.1.
BC034049 mRNA. Translation: AAH34049.1.
BK001284 mRNA. Translation: DAA01323.1.
CCDSiCCDS1716.1. [Q9NZQ0-1]
CCDS74493.1. [Q9NZQ0-3]
RefSeqiNP_001185488.1. NM_001198559.1. [Q9NZQ0-3]
NP_057628.1. NM_016544.2. [Q9NZQ0-1]
UniGeneiHs.434993.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YS8NMR-A197-273[»]
ProteinModelPortaliQ9NZQ0.
SMRiQ9NZQ0. Positions 17-177, 201-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119429. 1 interaction.
IntActiQ9NZQ0. 1 interaction.
STRINGi9606.ENSP00000264711.

PTM databases

iPTMnetiQ9NZQ0.
PhosphoSiteiQ9NZQ0.

Polymorphism and mutation databases

BioMutaiDNAJC27.
DMDMi74734733.

Proteomic databases

PaxDbiQ9NZQ0.
PeptideAtlasiQ9NZQ0.
PRIDEiQ9NZQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264711; ENSP00000264711; ENSG00000115137. [Q9NZQ0-1]
ENST00000380809; ENSP00000370187; ENSG00000115137. [Q9NZQ0-2]
ENST00000534855; ENSP00000440086; ENSG00000115137. [Q9NZQ0-3]
GeneIDi51277.
KEGGihsa:51277.
UCSCiuc002rft.2. human. [Q9NZQ0-1]

Organism-specific databases

CTDi51277.
GeneCardsiDNAJC27.
H-InvDBHIX0001881.
HGNCiHGNC:30290. DNAJC27.
HPAiHPA036815.
HPA036816.
MIMi613527. gene.
neXtProtiNX_Q9NZQ0.
PharmGKBiPA164718860.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0098. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233969.
HOVERGENiHBG056504.
InParanoidiQ9NZQ0.
KOiK19372.
OMAiFYVSIVD.
OrthoDBiEOG7J70GQ.
PhylomeDBiQ9NZQ0.
TreeFamiTF328564.

Miscellaneous databases

EvolutionaryTraceiQ9NZQ0.
GenomeRNAii51277.
PROiQ9NZQ0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NZQ0.
CleanExiHS_DNAJC27.
GenevisibleiQ9NZQ0. HS.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a unique Rab GTPase containing a J domain that interacts with Hsc70."
    Chen T., Zhang W., Li N., Wan T., Zhang M., Chen G., Zhang Y., Cao X.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. Cited for: IDENTIFICATION, POSSIBLE LACK OF GTPASE ACTIVITY.
  6. "Small GTPase RBJ mediates nuclear entrapment of MEK1/MEK2 in tumor progression."
    Chen T., Yang M., Yu Z., Tang S., Wang C., Zhu X., Guo J., Li N., Zhang W., Hou J., Liu H., Han C., Liu Q., Gu Y., Qian C., Wan T., Cui L., Zhu M., Zheng W., Cao X.
    Cancer Cell 25:682-696(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Solution structure of the dnaJ-like domain from human Ras-associated protein RAP1."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 197-273.

Entry informationi

Entry nameiDJC27_HUMAN
AccessioniPrimary (citable) accession number: Q9NZQ0
Secondary accession number(s): Q5JV88, Q86Y24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

DNAJC27/RBJ knockdown in several colorectal cancer cell lines is correlated to inhibition of MEK/ERK activation, cell proliferation, colony formation and in vivo tumor growth.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.