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Protein

DnaJ homolog subfamily C member 27

Gene

DNAJC27

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 30GTPBy similarity8
Nucleotide bindingi71 – 75GTPBy similarity5
Nucleotide bindingi134 – 137GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115137-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 27
Alternative name(s):
Rab and DnaJ domain-containing protein
Gene namesi
Name:DNAJC27
Synonyms:RABJS, RBJ
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:30290. DNAJC27.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Oncogene

Organism-specific databases

DisGeNETi51277.
OpenTargetsiENSG00000115137.
PharmGKBiPA164718860.

Polymorphism and mutation databases

BioMutaiDNAJC27.
DMDMi74734733.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003329751 – 273DnaJ homolog subfamily C member 27Add BLAST273

Proteomic databases

PaxDbiQ9NZQ0.
PeptideAtlasiQ9NZQ0.
PRIDEiQ9NZQ0.

PTM databases

iPTMnetiQ9NZQ0.
PhosphoSitePlusiQ9NZQ0.

Expressioni

Tissue specificityi

Overexpressed in gastrointestinal cancers; expression correlates with later tumor-node-metastasis stages of colorectal cancers.1 Publication

Gene expression databases

BgeeiENSG00000115137.
CleanExiHS_DNAJC27.
GenevisibleiQ9NZQ0. HS.

Organism-specific databases

HPAiHPA036815.
HPA036816.

Interactioni

Subunit structurei

Interacts directly with MAPK1 (wild-type and kinase-deficient forms). Interacts directly (in GTP-bound form) with MAP2K1 (wild-type and kinase-deficient forms).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TFCP2Q128003EBI-10317544,EBI-717422

Protein-protein interaction databases

BioGridi119429. 1 interactor.
IntActiQ9NZQ0. 1 interactor.
STRINGi9606.ENSP00000264711.

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi203 – 213Combined sources11
Helixi218 – 222Combined sources5
Helixi230 – 244Combined sources15
Turni246 – 248Combined sources3
Helixi254 – 272Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YS8NMR-A197-273[»]
ProteinModelPortaliQ9NZQ0.
SMRiQ9NZQ0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NZQ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini217 – 273JPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 18Required for interaction with MAPK1By similarityAdd BLAST18

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated
Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0098. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133872.
HOGENOMiHOG000233969.
HOVERGENiHBG056504.
InParanoidiQ9NZQ0.
KOiK19372.
OMAiFYVSIVD.
OrthoDBiEOG091G0IXD.
PhylomeDBiQ9NZQ0.
TreeFamiTF328564.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZQ0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEANMPKRKE PGRSLRIKVI SMGNAEVGKS CIIKRYCEKR FVSKYLATIG
60 70 80 90 100
IDYGVTKVHV RDREIKVNIF DMAGHPFFYE VRNEFYKDTQ GVILVYDVGQ
110 120 130 140 150
KDSFDALDAW LAEMKQELGP HGNMENIIFV VCANKIDCTK HRCVDESEGR
160 170 180 190 200
LWAESKGFLY FETSAQTGEG INEMFQTFYI SIVDLCENGG KRPTTNSSAS
210 220 230 240 250
FTKEQADAIR RIRNSKDSWD MLGVKPGASR DEVNKAYRKL AVLLHPDKCV
260 270
APGSEDAFKA VVNARTALLK NIK
Length:273
Mass (Da):30,855
Last modified:October 1, 2000 - v1
Checksum:iA3A0CE43EA6A2BCA
GO
Isoform 2 (identifier: Q9NZQ0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     81-90: VRNEFYKDTQ → MRKLRRREVK
     91-273: Missing.

Show »
Length:90
Mass (Da):10,622
Checksum:i24A41685FE2A3C94
GO
Isoform 3 (identifier: Q9NZQ0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     177-177: T → G
     178-273: Missing.

Show »
Length:177
Mass (Da):20,264
Checksum:iEECCC11FF0F5130B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03340981 – 90VRNEFYKDTQ → MRKLRRREVK in isoform 2. 1 Publication10
Alternative sequenceiVSP_03341091 – 273Missing in isoform 2. 1 PublicationAdd BLAST183
Alternative sequenceiVSP_033411177T → G in isoform 3. 1 Publication1
Alternative sequenceiVSP_033412178 – 273Missing in isoform 3. 1 PublicationAdd BLAST96

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178983 mRNA. Translation: AAF44347.1.
AY094594 mRNA. Translation: AAM22521.1.
AL137731 mRNA. Translation: CAI46214.1.
CH471053 Genomic DNA. Translation: EAX00736.1.
CH471053 Genomic DNA. Translation: EAX00735.1.
CH471053 Genomic DNA. Translation: EAX00737.1.
BC034049 mRNA. Translation: AAH34049.1.
BK001284 mRNA. Translation: DAA01323.1.
CCDSiCCDS1716.1. [Q9NZQ0-1]
CCDS74493.1. [Q9NZQ0-3]
RefSeqiNP_001185488.1. NM_001198559.1. [Q9NZQ0-3]
NP_057628.1. NM_016544.2. [Q9NZQ0-1]
UniGeneiHs.434993.

Genome annotation databases

EnsembliENST00000264711; ENSP00000264711; ENSG00000115137. [Q9NZQ0-1]
ENST00000380809; ENSP00000370187; ENSG00000115137. [Q9NZQ0-2]
ENST00000534855; ENSP00000440086; ENSG00000115137. [Q9NZQ0-3]
GeneIDi51277.
KEGGihsa:51277.
UCSCiuc002rft.2. human. [Q9NZQ0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF178983 mRNA. Translation: AAF44347.1.
AY094594 mRNA. Translation: AAM22521.1.
AL137731 mRNA. Translation: CAI46214.1.
CH471053 Genomic DNA. Translation: EAX00736.1.
CH471053 Genomic DNA. Translation: EAX00735.1.
CH471053 Genomic DNA. Translation: EAX00737.1.
BC034049 mRNA. Translation: AAH34049.1.
BK001284 mRNA. Translation: DAA01323.1.
CCDSiCCDS1716.1. [Q9NZQ0-1]
CCDS74493.1. [Q9NZQ0-3]
RefSeqiNP_001185488.1. NM_001198559.1. [Q9NZQ0-3]
NP_057628.1. NM_016544.2. [Q9NZQ0-1]
UniGeneiHs.434993.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YS8NMR-A197-273[»]
ProteinModelPortaliQ9NZQ0.
SMRiQ9NZQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119429. 1 interactor.
IntActiQ9NZQ0. 1 interactor.
STRINGi9606.ENSP00000264711.

PTM databases

iPTMnetiQ9NZQ0.
PhosphoSitePlusiQ9NZQ0.

Polymorphism and mutation databases

BioMutaiDNAJC27.
DMDMi74734733.

Proteomic databases

PaxDbiQ9NZQ0.
PeptideAtlasiQ9NZQ0.
PRIDEiQ9NZQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264711; ENSP00000264711; ENSG00000115137. [Q9NZQ0-1]
ENST00000380809; ENSP00000370187; ENSG00000115137. [Q9NZQ0-2]
ENST00000534855; ENSP00000440086; ENSG00000115137. [Q9NZQ0-3]
GeneIDi51277.
KEGGihsa:51277.
UCSCiuc002rft.2. human. [Q9NZQ0-1]

Organism-specific databases

CTDi51277.
DisGeNETi51277.
GeneCardsiDNAJC27.
H-InvDBHIX0001881.
HGNCiHGNC:30290. DNAJC27.
HPAiHPA036815.
HPA036816.
MIMi613527. gene.
neXtProtiNX_Q9NZQ0.
OpenTargetsiENSG00000115137.
PharmGKBiPA164718860.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0098. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133872.
HOGENOMiHOG000233969.
HOVERGENiHBG056504.
InParanoidiQ9NZQ0.
KOiK19372.
OMAiFYVSIVD.
OrthoDBiEOG091G0IXD.
PhylomeDBiQ9NZQ0.
TreeFamiTF328564.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115137-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9NZQ0.
GenomeRNAii51277.
PROiQ9NZQ0.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115137.
CleanExiHS_DNAJC27.
GenevisibleiQ9NZQ0. HS.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDJC27_HUMAN
AccessioniPrimary (citable) accession number: Q9NZQ0
Secondary accession number(s): Q5JV88, Q86Y24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

DNAJC27/RBJ knockdown in several colorectal cancer cell lines is correlated to inhibition of MEK/ERK activation, cell proliferation, colony formation and in vivo tumor growth.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.