Q9NZN8 (CNOT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCR4-NOT transcription complex subunit 2

Alternative name(s):
CCR4-associated factor 2

Gene names

Name:	CNOT2
Synonyms:	CDC36, NOT2
ORF Names:	HSPC131, MSTP046

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	540 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The CCR4-NOT complex functions as general transcription regulation complex.
Subunit structure	Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8.
Subcellular location	Cytoplasm Probable. Nucleus Probable.
Tissue specificity	Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Sequence similarities	Belongs to the CNOT2/3/5 family.
Sequence caution	The sequence AAF29095.1 differs from that shown. Reason: Frameshift at position 474. The sequence AAQ13426.1 differs from that shown. Reason: Frameshift at positions 71, 76, 84, 98, 123, 370, 384 and 393.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear-transcribed mRNA poly(A) tail shortening Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Molecular function	RNA polymerase II transcription cofactor activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NZN8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NZN8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NZN8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-349: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Isoform 4 (identifier: Q9NZN8-4)

The sequence of this isoform differs from the canonical sequence as follows:
     465-475: FNRDWRYHKEE → YVQSILITFVL
     476-540: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Isoform 5 (identifier: Q9NZN8-5)

The sequence of this isoform differs from the canonical sequence as follows:
     229-278: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 540	540	CCR4-NOT transcription complex subunit 2		PRO_0000198331
Amino acid modifications
	Modified residue	93	1	Phosphothreonine Ref.10
	Modified residue	101	1	Phosphoserine Ref.8
	Modified residue	126	1	Phosphoserine Ref.12
	Modified residue	165	1	Phosphoserine Ref.9 Ref.10 Ref.11 Ref.12
Natural variations
	Alternative sequence	1 – 349	349	Missing in isoform 3.		VSP_009913
	Alternative sequence	1 – 175	175	Missing in isoform 2.		VSP_009912
	Alternative sequence	229 – 278	50	Missing in isoform 5.		VSP_009914
	Alternative sequence	465 – 475	11	FNRDWRYHKEE → YVQSILITFVL in isoform 4.		VSP_009915
	Alternative sequence	476 – 540	65	Missing in isoform 4.		VSP_009916
	Natural variant	460	1	A → T. Corresponds to variant rs11178192 [ dbSNP | Ensembl ].		VAR_048750

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7183890F033E3B83	FASTA	540	59,738
	10 20 30 40 50 60 MVRTDGHTLS EKRNYQVTNS MFGASRKKFV EGVDSDYHDE NMYYSQSSMF PHRSEKDMLA 70 80 90 100 110 120 SPSTSGQLSQ FGASLYGQQS ALGLPMRGMS NNTPQLNRSL SQGTQLPSHV TPTTGVPTMS 130 140 150 160 170 180 LHTPPSPSRG ILPMNPRNMM NHSQVGQGIG IPSRTNSMSS SGLGSPNRSS PSIICMPKQQ 190 200 210 220 230 240 PSRQPFTVNS MSGFGMNRNQ AFGMNNSLSS NIFNGTDGSE NVTGLDLSDF PALADRNRRE 250 260 270 280 290 300 GSGNPTPLIN PLAGRAPYVG MVTKPANEQS QDFSIHNEDF PALPGSSYKD PTSSNDDSKS 310 320 330 340 350 360 NLNTSGKTTS STDGPKFPGD KSSTTQNNNQ QKKGIQVLPD GRVTNIPQGM VTDQFGMIGL 370 380 390 400 410 420 LTFIRAAETD PGMVHLALGS DLTTLGLNLN SPENLYPKFA SPWASSPCRP QDIDFHVPSE 430 440 450 460 470 480 YLTNIHIRDK LAAIKLGRYG EDLLFYLYYM NGGDVLQLLA AVELFNRDWR YHKEERVWIT 490 500 510 520 530 540 RAPGMEPTMK TNTYERGTYY FFDCLNWRKV AKEFHLEYDK LEERPHLPST FNYNPAQQAF
	Isoform 2 [UniParc]. Checksum: 27FC908ADA1A6201	FASTA	365	40,818
	10 20 30 40 50 60 MPKQQPSRQP FTVNSMSGFG MNRNQAFGMN NSLSSNIFNG TDGSENVTGL DLSDFPALAD 70 80 90 100 110 120 RNRREGSGNP TPLINPLAGR APYVGMVTKP ANEQSQDFSI HNEDFPALPG SSYKDPTSSN 130 140 150 160 170 180 DDSKSNLNTS GKTTSSTDGP KFPGDKSSTT QNNNQQKKGI QVLPDGRVTN IPQGMVTDQF 190 200 210 220 230 240 GMIGLLTFIR AAETDPGMVH LALGSDLTTL GLNLNSPENL YPKFASPWAS SPCRPQDIDF 250 260 270 280 290 300 HVPSEYLTNI HIRDKLAAIK LGRYGEDLLF YLYYMNGGDV LQLLAAVELF NRDWRYHKEE 310 320 330 340 350 360 RVWITRAPGM EPTMKTNTYE RGTYYFFDCL NWRKVAKEFH LEYDKLEERP HLPSTFNYNP AQQAF
	Isoform 3 [UniParc]. Checksum: 803037A4A6F670CC	FASTA	191	22,254
	10 20 30 40 50 60 MVTDQFGMIG LLTFIRAAET DPGMVHLALG SDLTTLGLNL NSPENLYPKF ASPWASSPCR 70 80 90 100 110 120 PQDIDFHVPS EYLTNIHIRD KLAAIKLGRY GEDLLFYLYY MNGGDVLQLL AAVELFNRDW 130 140 150 160 170 180 RYHKEERVWI TRAPGMEPTM KTNTYERGTY YFFDCLNWRK VAKEFHLEYD KLEERPHLPS 190 TFNYNPAQQA F
	Isoform 4 [UniParc]. Checksum: 5D5C630BB3E89F06	FASTA	475	51,537
	10 20 30 40 50 60 MVRTDGHTLS EKRNYQVTNS MFGASRKKFV EGVDSDYHDE NMYYSQSSMF PHRSEKDMLA 70 80 90 100 110 120 SPSTSGQLSQ FGASLYGQQS ALGLPMRGMS NNTPQLNRSL SQGTQLPSHV TPTTGVPTMS 130 140 150 160 170 180 LHTPPSPSRG ILPMNPRNMM NHSQVGQGIG IPSRTNSMSS SGLGSPNRSS PSIICMPKQQ 190 200 210 220 230 240 PSRQPFTVNS MSGFGMNRNQ AFGMNNSLSS NIFNGTDGSE NVTGLDLSDF PALADRNRRE 250 260 270 280 290 300 GSGNPTPLIN PLAGRAPYVG MVTKPANEQS QDFSIHNEDF PALPGSSYKD PTSSNDDSKS 310 320 330 340 350 360 NLNTSGKTTS STDGPKFPGD KSSTTQNNNQ QKKGIQVLPD GRVTNIPQGM VTDQFGMIGL 370 380 390 400 410 420 LTFIRAAETD PGMVHLALGS DLTTLGLNLN SPENLYPKFA SPWASSPCRP QDIDFHVPSE 430 440 450 460 470 YLTNIHIRDK LAAIKLGRYG EDLLFYLYYM NGGDVLQLLA AVELYVQSIL ITFVL
	Isoform 5 [UniParc]. Checksum: 7C6A4CC0F0992C9C	FASTA	490	54,306
	10 20 30 40 50 60 MVRTDGHTLS EKRNYQVTNS MFGASRKKFV EGVDSDYHDE NMYYSQSSMF PHRSEKDMLA 70 80 90 100 110 120 SPSTSGQLSQ FGASLYGQQS ALGLPMRGMS NNTPQLNRSL SQGTQLPSHV TPTTGVPTMS 130 140 150 160 170 180 LHTPPSPSRG ILPMNPRNMM NHSQVGQGIG IPSRTNSMSS SGLGSPNRSS PSIICMPKQQ 190 200 210 220 230 240 PSRQPFTVNS MSGFGMNRNQ AFGMNNSLSS NIFNGTDGSE NVTGLDLSDF PALPGSSYKD 250 260 270 280 290 300 PTSSNDDSKS NLNTSGKTTS STDGPKFPGD KSSTTQNNNQ QKKGIQVLPD GRVTNIPQGM 310 320 330 340 350 360 VTDQFGMIGL LTFIRAAETD PGMVHLALGS DLTTLGLNLN SPENLYPKFA SPWASSPCRP 370 380 390 400 410 420 QDIDFHVPSE YLTNIHIRDK LAAIKLGRYG EDLLFYLYYM NGGDVLQLLA AVELFNRDWR 430 440 450 460 470 480 YHKEERVWIT RAPGMEPTMK TNTYERGTYY FFDCLNWRKV AKEFHLEYDK LEERPHLPST 490 FNYNPAQQAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits." Albert T.K., Lemaire M., van Berkum N.L., Gentz R., Collart M.A., Timmers H.T.M. Nucleic Acids Res. 28:809-817(2000) [PubMed: 10637334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH CNOT1; CNOT2; CNOT3; CNOT4 AND CNOT8, TISSUE SPECIFICITY.
[2]	Liu B., Liu Y.Q., Wang X.Y., Zhao B., Sheng H., Zhao X.W., Liu S., Xu Y.Y., Ye J., Song L., Gao Y., Zhang C.L., Zhang J., Wei Y.J., Cao H.Q., Zhao Y., Liu L.S., Ding J.F. , Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Heart.
[3]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Umbilical cord blood.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: Fetal kidney and Testis.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lymph and Skin.
[7]	"Separation and molecular cloning of a novel human cDNA fragment coding a protein without homologs." Bi A., Yu L., Zhang M., Fan Y., Zhang Q., Zhao Y., Gao J., Zhao S. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-377.
[8]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-93 AND SER-165, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-126 AND SER-165, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF180473 mRNA. Translation: AAF29827.1. AF113226 mRNA. Translation: AAG39297.1. AF161480 mRNA. Translation: AAF29095.1. Frameshift. AK000662 mRNA. Translation: BAA91313.1. AL137674 mRNA. Translation: CAB70869.1. BX641116 mRNA. Translation: CAE46054.1. BC002597 mRNA. Translation: AAH02597.1. BC011826 mRNA. Translation: AAH11826.1. AF044215 mRNA. Translation: AAQ13426.1. Frameshift.
IPI	IPI00005011. IPI00017733. IPI00410621. IPI00410622. IPI00410623.
PIR	T46494.
RefSeq	NP_001186231.1. NM_001199302.1. NP_001186232.1. NM_001199303.1. NP_055330.1. NM_014515.5.
UniGene	Hs.133350.
3D structure databases
ProteinModelPortal	Q9NZN8.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-42656N.
IntAct	Q9NZN8. 7 interactions.
MINT	MINT-1438957.
STRING	Q9NZN8.
PTM databases
PhosphoSite	Q9NZN8.
Polymorphism databases
DMDM	46396017.
Proteomic databases
PRIDE	Q9NZN8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000229195; ENSP00000229195; ENSG00000111596.
GeneID	4848.
KEGG	hsa:4848.
UCSC	uc001svv.1. human. uc009zrp.1. human.
Organism-specific databases
CTD	4848.
GeneCards	GC12P070636.
HGNC	HGNC:7878. CNOT2.
MIM	604909. gene.
neXtProt	NX_Q9NZN8.
PharmGKB	PA26673.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05145.
GeneTree	ENSGT00390000001285.
HOGENOM	HBG445797.
HOVERGEN	HBG051041.
InParanoid	Q9NZN8.
OMA	MFGARKK.
OrthoDB	EOG44MXRV.
PhylomeDB	Q9NZN8.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q9NZN8.
Bgee	Q9NZN8.
CleanEx	HS_CNOT2.
Genevestigator	Q9NZN8.
GermOnline	ENSG00000111596. Homo sapiens.
Family and domain databases
InterPro	IPR007282. NOT. [Graphical view]
KO	K12605.
Pfam	PF04153. NOT2_3_5. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	18670.
SOURCE	Search...

Entry information

Entry name

CNOT2_HUMAN

Accession

Primary (citable) accession number: Q9NZN8
Secondary accession number(s): Q9H3E0 , Q9NSX5, Q9NWR6, Q9P028

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.