Q9NZN3 (EHD3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EH domain-containing protein 3

Alternative name(s):
PAST homolog 3

Gene names

Name:	EHD3
Synonyms:	EHD2, PAST3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in endocytic transport. Ref.6
Subunit structure	Interacts with PACSIN1 By similarity. Homooligomer, and heterooligomer with EHD1, EHD2 and EHD4. Ref.6 Ref.7
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Cytoplasm By similarity Ref.6.
Tissue specificity	Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.
Domain	The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins By similarity.
Sequence similarities	Contains 1 EF-hand domain. Contains 1 EH domain.
Sequence caution	The sequence AAF40471.1 differs from that shown. Reason: Frameshift at position 527.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Endosome Membrane
Domain	Coiled coil
Ligand	ATP-binding Calcium Nucleotide-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	blood coagulation Traceable author statement. Source: Reactome endocytic recycling Inferred from direct assay Ref.6. Source: UniProtKB protein homooligomerization Inferred from physical interaction Ref.6. Source: UniProtKB
Cellular component	nucleus Traceable author statement. Source: ProtInc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell recycling endosome membrane Inferred from direct assay Ref.6. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: InterPro GTPase activity Inferred from electronic annotation. Source: InterPro calcium ion binding Inferred from electronic annotation. Source: InterPro nucleic acid binding Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	EH domain-containing protein 3		PRO_0000146112
Regions
	Domain	444 – 532	89	EH
	Domain	476 – 511	36	EF-hand
	Nucleotide binding	65 – 72	8	ATP By similarity
	Calcium binding	489 – 500	12	By similarity
	Coiled coil	198 – 227	30	Potential
Sites
	Binding site	220	1	ATP By similarity
	Binding site	258	1	ATP By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.5
	Modified residue	456	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q9NZN3 [UniParc]. Last modified July 13, 2010. Version 2. Checksum: 0F8AE99F84B966AD	FASTA	535	60,887
	10 20 30 40 50 60 MFSWLGTDDR RRKDPEVFQT VSEGLKKLYK SKLLPLEEHY RFHEFHSPAL EDADFDNKPM 70 80 90 100 110 120 VLLVGQYSTG KTTFIRYLLE QDFPGMRIGP EPTTDSFIAV MQGDMEGIIP GNALVVDPKK 130 140 150 160 170 180 PFRKLNAFGN AFLNRFVCAQ LPNPVLESIS VIDTPGILSG EKQRISRGYD FAAVLEWFAE 190 200 210 220 230 240 RVDRIILLFD AHKLDISDEF SEVIKALKNH EDKMRVVLNK ADQIETQQLM RVYGALMWSL 250 260 270 280 290 300 GKIVNTPEVI RVYIGSFWSH PLLIPDNRKL FEAEEQDLFR DIQSLPRNAA LRKLNDLIKR 310 320 330 340 350 360 ARLAKVHAYI ISSLKKEMPS VFGKDNKKKE LVNNLAEIYG RIEREHQISP GDFPNLKRMQ 370 380 390 400 410 420 DQLQAQDFSK FQPLKSKLLE VVDDMLAHDI AQLMVLVRQE ESQRPIQMVK GGAFEGTLHG 430 440 450 460 470 480 PFGHGYGEGA GEGIDDAEWV VARDKPMYDE IFYTLSPVDG KITGANAKKE MVRSKLPNSV 490 500 510 520 530 LGKIWKLADI DKDGMLDDDE FALANHLIKV KLEGHELPNE LPAHLLPPSK RKVAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EHD2, EHD3, and EHD4 encode novel members of a highly conserved family of EH domain-containing proteins." Pohl U., Smith J.S., Tachibana I., Ueki K., Lee H.K., Ramaswamy S., Wu Q., Mohrenweiser H.W., Jenkins R.B., Louis D.N. Genomics 63:255-262(2000) [PubMed: 10673336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"EHD2-EH domain containing 2, homolog of EHD1." Galperin E., Benjamin S., Horowitz M. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, ACETYLATION AT MET-1. Tissue: Platelet.
[6]	"Shared as well as distinct roles of EHD proteins revealed by biochemical and functional comparisons in mammalian cells and C. elegans." George M., Ying G., Rainey M.A., Solomon A., Parikh P.T., Gao Q., Band V., Band H. BMC Cell Biol. 8:3-3(2007) [PubMed: 17233914] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
[7]	"A role for EHD4 in the regulation of early endosomal transport." Sharma M., Naslavsky N., Caplan S. Traffic 9:995-1018(2008) [PubMed: 18331452] [Abstract] Cited for: INTERACTION WITH EHD4.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF181264 mRNA. Translation: AAF40471.1. Frameshift. AF214736 mRNA. Translation: AAF32285.1. AL121657 Genomic DNA. No translation available. CH471053 Genomic DNA. Translation: EAX00480.1. CH471053 Genomic DNA. Translation: EAX00481.1.
IPI	IPI00021458.
RefSeq	NP_055415.1. NM_014600.2.
UniGene	Hs.368808.
3D structure databases
ProteinModelPortal	Q9NZN3.
SMR	Q9NZN3. Positions 19-532.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NZN3. 1 interaction.
STRING	Q9NZN3.
PTM databases
PhosphoSite	Q9NZN3.
Polymorphism databases
DMDM	300669636.
2D gel databases
OGP	Q9NZN3.
Proteomic databases
PRIDE	Q9NZN3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000322054; ENSP00000327116; ENSG00000013016.
GeneID	30845.
KEGG	hsa:30845.
Organism-specific databases
CTD	30845.
GeneCards	GC02P031368.
H-InvDB	HIX0001950.
HGNC	HGNC:3244. EHD3.
MIM	605891. gene.
neXtProt	NX_Q9NZN3.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06454.
GeneTree	ENSGT00600000084243.
HOGENOM	HBG317590.
HOVERGEN	HBG018183.
InParanoid	Q9NZN3.
OMA	KVWKLAD.
OrthoDB	EOG418BN4.
PhylomeDB	Q9NZN3.
Enzyme and pathway databases
Reactome	REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q9NZN3.
Bgee	Q9NZN3.
CleanEx	HS_EHD2. HS_EHD3.
Genevestigator	Q9NZN3.
GermOnline	ENSG00000013016. Homo sapiens.
Family and domain databases
InterPro	IPR001401. Dynamin_GTPase. IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR000261. EPS15_homology. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
KO	K12476.
Pfam	PF00350. Dynamin_N. 1 hit. [Graphical view]
SMART	SM00027. EH. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 1 hit. PS50031. EH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	52968.
SOURCE	Search...

Entry information

Entry name

EHD3_HUMAN

Accession

Primary (citable) accession number: Q9NZN3
Secondary accession number(s): D6W574, Q8N514, Q9NZB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	July 13, 2010
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.