Q9NZM3 (ITSN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Intersectin-2

Alternative name(s):
SH3 domain-containing protein 1B
SH3P18
SH3P18-like WASP-associated protein

Gene names

Name:	ITSN2
Synonyms:	KIAA1256, SH3D1B, SWAP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1697 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Ref.15 Ref.20
Subunit structure	Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process. Interacts with ADAM15. Interacts with FASLG. Interacts with ANKRD54 By similarity. Interacts with FCHO2. Ref.13 Ref.14 Ref.18
Subcellular location	Cytoplasm.
Tissue specificity	Ubiquitous. Isoform 1 is primarily expressed in adult heart and liver.
Miscellaneous	Overexpression results in the inhibition of the transferrin uptake and the blockage of the clathrin-mediated endocytosis.
Sequence similarities	Contains 1 C2 domain. Contains 1 DH (DBL-homology) domain. Contains 1 EF-hand domain. Contains 2 EH domains. Contains 1 PH domain. Contains 5 SH3 domains.
Caution	It is uncertain whether Met-1 or Met-2 is the initiator. Studies in clathrin-mediated endocytosis of ITGB1 and TFR used a siRNA mixture of ISTN1 and ISTN2 suggesting a partially overlapping role of the EH domain-containing proteins (Ref.20).
Sequence caution	The sequence AAF59903.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAF59904.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAF63600.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA86570.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat SH3 domain
Ligand	Calcium Metal-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro SH3/SH2 adaptor activity Traceable author statement Ref.7. Source: ProtInc calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9NZM3-1)

Also known as: ITSN2-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NZM3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     622-648: Missing.

Isoform 3 (identifier: Q9NZM3-3)

Also known as: ITSN2-S1;

The sequence of this isoform differs from the canonical sequence as follows:
     1235-1249: FQKRMAESGFLTEGE → RRLLLASSRGICCLS
     1250-1697: Missing.

Isoform 4 (identifier: Q9NZM3-4)

Also known as: ITSN2-S2; SH3P18;

The sequence of this isoform differs from the canonical sequence as follows:
     1193-1697: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1697

1697

Intersectin-2

PRO_0000080961

Regions

Domain

22 – 110

89

EH 1

Domain

54 – 89

36

EF-hand

Domain

244 – 333

90

EH 2

Domain

757 – 818

62

SH3 1

Domain

898 – 956

59

SH3 2

Domain

981 – 1039

59

SH3 3

Domain

1053 – 1117

65

SH3 4

Domain

1127 – 1186

60

SH3 5

Domain

1209 – 1395

187

DH

Domain

1434 – 1544

111

PH

Domain

1556 – 1652

97

C2

Calcium binding

67 – 79

13

Potential

Coiled coil

361 – 756

396

Potential

Amino acid modifications

Modified residue

210

1

Phosphoserine Ref.16

Modified residue

230

1

Phosphoserine Ref.17

Modified residue

553

1

Phosphotyrosine By similarity

Modified residue

573

1

Phosphothreonine Ref.17

Modified residue

889

1

Phosphoserine Ref.12 Ref.16 Ref.17

Modified residue

968

1

Phosphotyrosine Ref.9 Ref.10 Ref.16 Ref.17

Natural variations

Alternative sequence

622 – 648

27

Missing in isoform 2.

VSP_003892

Alternative sequence

1193 – 1697

505

Missing in isoform 4.

VSP_003895

Alternative sequence

1235 – 1249

15

FQKRM…LTEGE → RRLLLASSRGICCLS in isoform 3.

VSP_003893

Alternative sequence

1250 – 1697

448

Missing in isoform 3.

VSP_003894

Natural variant

254

1

T → A.
Corresponds to variant rs6744320 [ dbSNP | Ensembl ].

VAR_024287

Natural variant

291

1

V → I. Ref.5
Corresponds to variant rs7603997 [ dbSNP | Ensembl ].

VAR_024288

Natural variant

1287

1

I → T.
Corresponds to variant rs3731625 [ dbSNP | Ensembl ].

VAR_020193

Natural variant

1534

1

A → T.
Corresponds to variant rs2303291 [ dbSNP | Ensembl ].

VAR_021937

Experimental info

Sequence conflict

679

1

R → G in BAB13841. Ref.5

Sequence conflict

823 – 828

6

KAVSPK → FAAAST in AAD00899. Ref.6

Sequence conflict

945 – 951

7

WFPKSYV → EFAAAST in AAC50593. Ref.7

Sequence conflict

1279 – 1285

7

GEKMPVQ → VDAAANS in AAD00899. Ref.6

Sequence conflict

1553

1

K → Q in AAF63600. Ref.2

Sequence conflict

1553

1

K → Q in BAA86570. Ref.3

Isoform 3:

Sequence conflict

1235

1

R → W in AAF59904. Ref.1

Secondary structure

1

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1697

Helix Strand Turn

Details...

Beta strand

762 – 766

5

Beta strand

772 – 774

3

Beta strand

783 – 786

4

Beta strand

793 – 801

9

Beta strand

804 – 809

6

Beta strand

812 – 815

4

Beta strand

818 – 821

4

Beta strand

902 – 907

6

Beta strand

924 – 930

7

Beta strand

932 – 939

8

Beta strand

945 – 947

3

Helix

948 – 950

3

Beta strand

951 – 953

3

Beta strand

983 – 987

5

Beta strand

995 – 999

5

Beta strand

1007 – 1010

4

Beta strand

1012 – 1014

3

Beta strand

1017 – 1020

4

Beta strand

1027 – 1029

3

Helix

1031 – 1033

3

Beta strand

1056 – 1060

5

Beta strand

1079 – 1085

7

Beta strand

1087 – 1095

9

Beta strand

1104 – 1108

5

Beta strand

1111 – 1114

4

Beta strand

1129 – 1136

8

Beta strand

1153 – 1156

4

Beta strand

1161 – 1166

6

Beta strand

1168 – 1171

4

Beta strand

1180 – 1183

4

Helix

1207 – 1234

28

Helix

1236 – 1242

7

Beta strand

1243 – 1245

3

Helix

1247 – 1254

8

Helix

1257 – 1272

16

Helix

1288 – 1294

7

Helix

1299 – 1321

23

Helix

1323 – 1333

11

Turn

1336 – 1340

5

Helix

1343 – 1346

4

Helix

1349 – 1366

18

Helix

1375 – 1393

19

Beta strand

1569 – 1580

12

Beta strand

1592 – 1598

7

Beta strand

1601 – 1604

4

Beta strand

1615 – 1625

11

Turn

1627 – 1629

3

Beta strand

1631 – 1638

8

Beta strand

1641 – 1644

4

Beta strand

1647 – 1654

8

Helix

1655 – 1665

11

Beta strand

1670 – 1673

4

Beta strand

1675 – 1679

5

Beta strand

1681 – 1690

10

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ITSN2-L) [UniParc]. Last modified April 20, 2010. Version 3. Checksum: 49C41AE1E32E1BD0	FASTA	1,697	193,461
	10 20 30 40 50 60 MMAQFPTAMN GGPNMWAITS EERTKHDRQF DNLKPSGGYI TGDQARNFFL QSGLPAPVLA 70 80 90 100 110 120 EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP PIMKQPPMFS PLISARFGMG 130 140 150 160 170 180 SMPNLSIPQP LPPAAPITSL SSATSGTNLP PLMMPTPLVP SVSTSSLPNG TASLIQPLPI 190 200 210 220 230 240 PYSSSTLPHG SSYSLMMGGF GGASIQKAQS LIDLGSSSST SSTASLSGNS PKTGTSEWAV 250 260 270 280 290 300 PQPTRLKYRQ KFNTLDKSMS GYLSGFQARN ALLQSNLSQT QLATIWTLAD VDGDGQLKAE 310 320 330 340 350 360 EFILAMHLTD MAKAGQPLPL TLPPELVPPS FRGGKQIDSI NGTLPSYQKM QEEEPQKKLP 370 380 390 400 410 420 VTFEDKRKAN YERGNMELEK RRQALMEQQQ REAERKAQKE KEEWERKQRE LQEQEWKKQL 430 440 450 460 470 480 ELEKRLEKQR ELERQREEER RKDIERREAA KQELERQRRL EWERIRRQEL LNQKNREQEE 490 500 510 520 530 540 IVRLNSKKKN LHLELEALNG KHQQISGRLQ DVRLKKQTQK TELEVLDKQC DLEIMEIKQL 550 560 570 580 590 600 QQELQEYQNK LIYLVPEKQL LNERIKNMQF SNTPDSGVSL LHKKSLEKEE LCQRLKEQLD 610 620 630 640 650 660 ALEKETASKL SEMDSFNNQL KCGNMDDSVL QCLLSLLSCL NNLFLLLKEL RETYNTQQLA 670 680 690 700 710 720 LEQLYKIKRD KLKEIERKRL ELMQKKKLED EAARKAKQGK ENLWKENLRK EEEEKQKRLQ 730 740 750 760 770 780 EEKTQEKIQE EERKAEEKQR KDKDTLKAEE KKRETASVLV NYRALYPFEA RNHDEMSFNS 790 800 810 820 830 840 GDIIQVDEKT VGEPGWLYGS FQGNFGWFPC NYVEKMPSSE NEKAVSPKKA LLPPTVSLSA 850 860 870 880 890 900 TSTSSEPLSS NQPASVTDYQ NVSFSNLTVN TSWQKKSAFT RTVSPGSVSP IHGQGQVVEN 910 920 930 940 950 960 LKAQALCSWT AKKDNHLNFS KHDIITVLEQ QENWWFGEVH GGRGWFPKSY VKIIPGSEVK 970 980 990 1000 1010 1020 REEPEALYAA VNKKPTSAAY SVGEEYIALY PYSSVEPGDL TFTEGEEILV TQKDGEWWTG 1030 1040 1050 1060 1070 1080 SIGDRSGIFP SNYVKPKDQE SFGSASKSGA SNKKPEIAQV TSAYVASGSE QLSLAPGQLI 1090 1100 1110 1120 1130 1140 LILKKNTSGW WQGELQARGK KRQKGWFPAS HVKLLGPSSE RATPAFHPVC QVIAMYDYAA 1150 1160 1170 1180 1190 1200 NNEDELSFSK GQLINVMNKD DPDWWQGEIN GVTGLFPSNY VKMTTDSDPS QQWCADLQTL 1210 1220 1230 1240 1250 1260 DTMQPIERKR QGYIHELIQT EERYMADLQL VVEVFQKRMA ESGFLTEGEM ALIFVNWKEL 1270 1280 1290 1300 1310 1320 IMSNTKLLKA LRVRKKTGGE KMPVQMIGDI LAAELSHMQA YIRFCSCQLN GAALLQQKTD 1330 1340 1350 1360 1370 1380 EDTDFKEFLK KLASDPRCKG MPLSSFLLKP MQRITRYPLL IRSILENTPE SHADHSSLKL 1390 1400 1410 1420 1430 1440 ALERAEELCS QVNEGVREKE NSDRLEWIQA HVQCEGLAEQ LIFNSLTNCL GPRKLLHSGK 1450 1460 1470 1480 1490 1500 LYKTKSNKEL HGFLFNDFLL LTYMVKQFAV SSGSEKLFSS KSNAQFKMYK TPIFLNEVLV 1510 1520 1530 1540 1550 1560 KLPTDPSSDE PVFHISHIDR VYTLRTDNIN ERTAWVQKIK AASEQYIDTE KKKREKAYQA 1570 1580 1590 1600 1610 1620 RSQKTSGIGR LMVHVIEATE LKACKPNGKS NPYCEISMGS QSYTTRTIQD TLNPKWNFNC 1630 1640 1650 1660 1670 1680 QFFIKDLYQD VLCLTLFDRD QFSPDDFLGR TEIPVAKIRT EQESKGPMTR RLLLHEVPTG 1690 EVWVRFDLQL FEQKTLL
	Isoform 2 [UniParc]. Checksum: 91E65C08E5AB26F1	FASTA	1,670	190,496
	10 20 30 40 50 60 MMAQFPTAMN GGPNMWAITS EERTKHDRQF DNLKPSGGYI TGDQARNFFL QSGLPAPVLA 70 80 90 100 110 120 EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP PIMKQPPMFS PLISARFGMG 130 140 150 160 170 180 SMPNLSIPQP LPPAAPITSL SSATSGTNLP PLMMPTPLVP SVSTSSLPNG TASLIQPLPI 190 200 210 220 230 240 PYSSSTLPHG SSYSLMMGGF GGASIQKAQS LIDLGSSSST SSTASLSGNS PKTGTSEWAV 250 260 270 280 290 300 PQPTRLKYRQ KFNTLDKSMS GYLSGFQARN ALLQSNLSQT QLATIWTLAD VDGDGQLKAE 310 320 330 340 350 360 EFILAMHLTD MAKAGQPLPL TLPPELVPPS FRGGKQIDSI NGTLPSYQKM QEEEPQKKLP 370 380 390 400 410 420 VTFEDKRKAN YERGNMELEK RRQALMEQQQ REAERKAQKE KEEWERKQRE LQEQEWKKQL 430 440 450 460 470 480 ELEKRLEKQR ELERQREEER RKDIERREAA KQELERQRRL EWERIRRQEL LNQKNREQEE 490 500 510 520 530 540 IVRLNSKKKN LHLELEALNG KHQQISGRLQ DVRLKKQTQK TELEVLDKQC DLEIMEIKQL 550 560 570 580 590 600 QQELQEYQNK LIYLVPEKQL LNERIKNMQF SNTPDSGVSL LHKKSLEKEE LCQRLKEQLD 610 620 630 640 650 660 ALEKETASKL SEMDSFNNQL KELRETYNTQ QLALEQLYKI KRDKLKEIER KRLELMQKKK 670 680 690 700 710 720 LEDEAARKAK QGKENLWKEN LRKEEEEKQK RLQEEKTQEK IQEEERKAEE KQRKDKDTLK 730 740 750 760 770 780 AEEKKRETAS VLVNYRALYP FEARNHDEMS FNSGDIIQVD EKTVGEPGWL YGSFQGNFGW 790 800 810 820 830 840 FPCNYVEKMP SSENEKAVSP KKALLPPTVS LSATSTSSEP LSSNQPASVT DYQNVSFSNL 850 860 870 880 890 900 TVNTSWQKKS AFTRTVSPGS VSPIHGQGQV VENLKAQALC SWTAKKDNHL NFSKHDIITV 910 920 930 940 950 960 LEQQENWWFG EVHGGRGWFP KSYVKIIPGS EVKREEPEAL YAAVNKKPTS AAYSVGEEYI 970 980 990 1000 1010 1020 ALYPYSSVEP GDLTFTEGEE ILVTQKDGEW WTGSIGDRSG IFPSNYVKPK DQESFGSASK 1030 1040 1050 1060 1070 1080 SGASNKKPEI AQVTSAYVAS GSEQLSLAPG QLILILKKNT SGWWQGELQA RGKKRQKGWF 1090 1100 1110 1120 1130 1140 PASHVKLLGP SSERATPAFH PVCQVIAMYD YAANNEDELS FSKGQLINVM NKDDPDWWQG 1150 1160 1170 1180 1190 1200 EINGVTGLFP SNYVKMTTDS DPSQQWCADL QTLDTMQPIE RKRQGYIHEL IQTEERYMAD 1210 1220 1230 1240 1250 1260 LQLVVEVFQK RMAESGFLTE GEMALIFVNW KELIMSNTKL LKALRVRKKT GGEKMPVQMI 1270 1280 1290 1300 1310 1320 GDILAAELSH MQAYIRFCSC QLNGAALLQQ KTDEDTDFKE FLKKLASDPR CKGMPLSSFL 1330 1340 1350 1360 1370 1380 LKPMQRITRY PLLIRSILEN TPESHADHSS LKLALERAEE LCSQVNEGVR EKENSDRLEW 1390 1400 1410 1420 1430 1440 IQAHVQCEGL AEQLIFNSLT NCLGPRKLLH SGKLYKTKSN KELHGFLFND FLLLTYMVKQ 1450 1460 1470 1480 1490 1500 FAVSSGSEKL FSSKSNAQFK MYKTPIFLNE VLVKLPTDPS SDEPVFHISH IDRVYTLRTD 1510 1520 1530 1540 1550 1560 NINERTAWVQ KIKAASEQYI DTEKKKREKA YQARSQKTSG IGRLMVHVIE ATELKACKPN 1570 1580 1590 1600 1610 1620 GKSNPYCEIS MGSQSYTTRT IQDTLNPKWN FNCQFFIKDL YQDVLCLTLF DRDQFSPDDF 1630 1640 1650 1660 1670 LGRTEIPVAK IRTEQESKGP MTRRLLLHEV PTGEVWVRFD LQLFEQKTLL
	Isoform 3 (ITSN2-S1) [UniParc]. Checksum: 271797AF71A0EB28	FASTA	1,249	141,820
	10 20 30 40 50 60 MMAQFPTAMN GGPNMWAITS EERTKHDRQF DNLKPSGGYI TGDQARNFFL QSGLPAPVLA 70 80 90 100 110 120 EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP PIMKQPPMFS PLISARFGMG 130 140 150 160 170 180 SMPNLSIPQP LPPAAPITSL SSATSGTNLP PLMMPTPLVP SVSTSSLPNG TASLIQPLPI 190 200 210 220 230 240 PYSSSTLPHG SSYSLMMGGF GGASIQKAQS LIDLGSSSST SSTASLSGNS PKTGTSEWAV 250 260 270 280 290 300 PQPTRLKYRQ KFNTLDKSMS GYLSGFQARN ALLQSNLSQT QLATIWTLAD VDGDGQLKAE 310 320 330 340 350 360 EFILAMHLTD MAKAGQPLPL TLPPELVPPS FRGGKQIDSI NGTLPSYQKM QEEEPQKKLP 370 380 390 400 410 420 VTFEDKRKAN YERGNMELEK RRQALMEQQQ REAERKAQKE KEEWERKQRE LQEQEWKKQL 430 440 450 460 470 480 ELEKRLEKQR ELERQREEER RKDIERREAA KQELERQRRL EWERIRRQEL LNQKNREQEE 490 500 510 520 530 540 IVRLNSKKKN LHLELEALNG KHQQISGRLQ DVRLKKQTQK TELEVLDKQC DLEIMEIKQL 550 560 570 580 590 600 QQELQEYQNK LIYLVPEKQL LNERIKNMQF SNTPDSGVSL LHKKSLEKEE LCQRLKEQLD 610 620 630 640 650 660 ALEKETASKL SEMDSFNNQL KCGNMDDSVL QCLLSLLSCL NNLFLLLKEL RETYNTQQLA 670 680 690 700 710 720 LEQLYKIKRD KLKEIERKRL ELMQKKKLED EAARKAKQGK ENLWKENLRK EEEEKQKRLQ 730 740 750 760 770 780 EEKTQEKIQE EERKAEEKQR KDKDTLKAEE KKRETASVLV NYRALYPFEA RNHDEMSFNS 790 800 810 820 830 840 GDIIQVDEKT VGEPGWLYGS FQGNFGWFPC NYVEKMPSSE NEKAVSPKKA LLPPTVSLSA 850 860 870 880 890 900 TSTSSEPLSS NQPASVTDYQ NVSFSNLTVN TSWQKKSAFT RTVSPGSVSP IHGQGQVVEN 910 920 930 940 950 960 LKAQALCSWT AKKDNHLNFS KHDIITVLEQ QENWWFGEVH GGRGWFPKSY VKIIPGSEVK 970 980 990 1000 1010 1020 REEPEALYAA VNKKPTSAAY SVGEEYIALY PYSSVEPGDL TFTEGEEILV TQKDGEWWTG 1030 1040 1050 1060 1070 1080 SIGDRSGIFP SNYVKPKDQE SFGSASKSGA SNKKPEIAQV TSAYVASGSE QLSLAPGQLI 1090 1100 1110 1120 1130 1140 LILKKNTSGW WQGELQARGK KRQKGWFPAS HVKLLGPSSE RATPAFHPVC QVIAMYDYAA 1150 1160 1170 1180 1190 1200 NNEDELSFSK GQLINVMNKD DPDWWQGEIN GVTGLFPSNY VKMTTDSDPS QQWCADLQTL 1210 1220 1230 1240 DTMQPIERKR QGYIHELIQT EERYMADLQL VVEVRRLLLA SSRGICCLS
	Isoform 4 (ITSN2-S2) (SH3P18) [UniParc]. Checksum: 2DA2049587CF4082	FASTA	1,192	135,144
	10 20 30 40 50 60 MMAQFPTAMN GGPNMWAITS EERTKHDRQF DNLKPSGGYI TGDQARNFFL QSGLPAPVLA 70 80 90 100 110 120 EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP PIMKQPPMFS PLISARFGMG 130 140 150 160 170 180 SMPNLSIPQP LPPAAPITSL SSATSGTNLP PLMMPTPLVP SVSTSSLPNG TASLIQPLPI 190 200 210 220 230 240 PYSSSTLPHG SSYSLMMGGF GGASIQKAQS LIDLGSSSST SSTASLSGNS PKTGTSEWAV 250 260 270 280 290 300 PQPTRLKYRQ KFNTLDKSMS GYLSGFQARN ALLQSNLSQT QLATIWTLAD VDGDGQLKAE 310 320 330 340 350 360 EFILAMHLTD MAKAGQPLPL TLPPELVPPS FRGGKQIDSI NGTLPSYQKM QEEEPQKKLP 370 380 390 400 410 420 VTFEDKRKAN YERGNMELEK RRQALMEQQQ REAERKAQKE KEEWERKQRE LQEQEWKKQL 430 440 450 460 470 480 ELEKRLEKQR ELERQREEER RKDIERREAA KQELERQRRL EWERIRRQEL LNQKNREQEE 490 500 510 520 530 540 IVRLNSKKKN LHLELEALNG KHQQISGRLQ DVRLKKQTQK TELEVLDKQC DLEIMEIKQL 550 560 570 580 590 600 QQELQEYQNK LIYLVPEKQL LNERIKNMQF SNTPDSGVSL LHKKSLEKEE LCQRLKEQLD 610 620 630 640 650 660 ALEKETASKL SEMDSFNNQL KCGNMDDSVL QCLLSLLSCL NNLFLLLKEL RETYNTQQLA 670 680 690 700 710 720 LEQLYKIKRD KLKEIERKRL ELMQKKKLED EAARKAKQGK ENLWKENLRK EEEEKQKRLQ 730 740 750 760 770 780 EEKTQEKIQE EERKAEEKQR KDKDTLKAEE KKRETASVLV NYRALYPFEA RNHDEMSFNS 790 800 810 820 830 840 GDIIQVDEKT VGEPGWLYGS FQGNFGWFPC NYVEKMPSSE NEKAVSPKKA LLPPTVSLSA 850 860 870 880 890 900 TSTSSEPLSS NQPASVTDYQ NVSFSNLTVN TSWQKKSAFT RTVSPGSVSP IHGQGQVVEN 910 920 930 940 950 960 LKAQALCSWT AKKDNHLNFS KHDIITVLEQ QENWWFGEVH GGRGWFPKSY VKIIPGSEVK 970 980 990 1000 1010 1020 REEPEALYAA VNKKPTSAAY SVGEEYIALY PYSSVEPGDL TFTEGEEILV TQKDGEWWTG 1030 1040 1050 1060 1070 1080 SIGDRSGIFP SNYVKPKDQE SFGSASKSGA SNKKPEIAQV TSAYVASGSE QLSLAPGQLI 1090 1100 1110 1120 1130 1140 LILKKNTSGW WQGELQARGK KRQKGWFPAS HVKLLGPSSE RATPAFHPVC QVIAMYDYAA 1150 1160 1170 1180 1190 NNEDELSFSK GQLINVMNKD DPDWWQGEIN GVTGLFPSNY VKMTTDSDPS QQ

References

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[1]	"Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis." Pucharcos C., Estivill X., de la Luna S. FEBS Lett. 478:43-51(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3). Tissue: Fetal brain and Fetal liver.
[2]	"Intersectin 2 (SH3D1B), human homolog of mouse Ese2 protein." Seifert M., Engel M., Welter C. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 259-721 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1262-1697, VARIANT ILE-291. Tissue: Embryo.
[6]	"SH3P18-like wasp associated protein (SWAP): a multiple SH3 domain containing protein that interacts with WASP." Wilson L.A., Fields D., Cruz L., Friesen J., Siminovitch K.A. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 822-1285 (ISOFORM 1).
[7]	"Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins." Sparks A.B., Hoffman N.G., McConnell S.J., Fowlkes D.M., Kay B.K. Nat. Biotechnol. 14:741-744(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 945-1192 (ISOFORM 4). Tissue: Bone marrow.
[8]	"The human intersectin genes and their spliced variants are differentially expressed." Pucharcos C., Casas C., Nadal M., Estivill X., de la Luna S. Biochim. Biophys. Acta 1521:1-11(2001) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING. Tissue: Brain and Fetal liver.
[9]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-968, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-968, MASS SPECTROMETRY.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-889, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening." Voss M., Lettau M., Janssen O. BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FASLG.
[14]	"Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins." Kleino I., Ortiz R.M., Yritys M., Huovila A.P., Saksela K. J. Cell. Biochem. 108:877-885(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ADAM15.
[15]	"Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pits." Mettlen M., Stoeber M., Loerke D., Antonescu C.N., Danuser G., Schmid S.L. Mol. Biol. Cell 20:3251-3260(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[16]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-210; SER-889 AND TYR-968, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[17]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230; THR-573; SER-889 AND TYR-968, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[18]	"FCHo proteins are nucleators of clathrin-mediated endocytosis." Henne W.M., Boucrot E., Meinecke M., Evergren E., Vallis Y., Mittal R., McMahon H.T. Science 328:1281-1284(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FCHO2.
[19]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[20]	"The clathrin adaptor Dab2 recruits EH domain scaffold proteins to regulate integrin beta1 endocytosis." Teckchandani A., Mulkearns E.E., Randolph T.W., Toida N., Cooper J.A. Mol. Biol. Cell 23:2905-2916(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[21]	"Solution structure of SH3 domains of human intersectin 2 (KIAA1256)." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 762-1187.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF182198 mRNA. Translation: AAF59903.1. Different initiation.
AF182199 mRNA. Translation: AAF59904.1. Different initiation.
AF248540 mRNA. Translation: AAF63600.1. Different initiation.
AB033082 mRNA. Translation: BAA86570.1. Different initiation.
AC008073 Genomic DNA. No translation available.
AC009228 Genomic DNA. No translation available.
AK021545 mRNA. Translation: BAB13841.1.
AK000302 mRNA. Translation: BAA91068.1.
AF001630 mRNA. Translation: AAD00899.1.
U61167 mRNA. Translation: AAC50593.1.

IPI

IPI00892957.
IPI00937782.
IPI00941398.
IPI00943185.

RefSeq

NP_006268.2. NM_006277.2.
NP_062541.3. NM_019595.3.
NP_671494.2. NM_147152.2.

UniGene

Hs.432562.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J3T	NMR	-	A	898-957	[»]
1UDL	NMR	-	A	1103-1186	[»]
1UE9	NMR	-	A	1056-1121	[»]
1UFF	NMR	-	A	762-841	[»]
1UHF	NMR	-	A	983-1037	[»]
3GF9	X-ray	2.50	A	1130-1406	[»]
3JZY	X-ray	1.56	A	1201-1692	[»]

ProteinModelPortal

Q9NZM3.

SMR

Q9NZM3. Positions 2-108, 236-332, 762-845, 898-1551, 1568-1691.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NZM3. 22 interactions.

MINT

MINT-1404321.

STRING

9606.ENSP00000347244.

PTM databases

PhosphoSite

Q9NZM3.

Polymorphism databases

DMDM

294862505.

Proteomic databases

PaxDb

Q9NZM3.

PRIDE

Q9NZM3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000355123; ENSP00000347244; ENSG00000198399.
ENST00000361999; ENSP00000354561; ENSG00000198399.
ENST00000380868; ENSP00000370250; ENSG00000198399.
ENST00000406921; ENSP00000384499; ENSG00000198399.

GeneID

50618.

KEGG

hsa:50618.

UCSC

uc002rfe.2. human.
uc002rff.2. human.
uc002rfg.3. human.

Organism-specific databases

CTD

50618.

GeneCards

GC02M024425.

H-InvDB

HIX0001876.

HGNC

HGNC:6184. ITSN2.

HPA

HPA036475.

MIM

604464. gene.

neXtProt

NX_Q9NZM3.

PharmGKB

PA29982.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5422.

HOVERGEN

HBG052159.

InParanoid

Q9NZM3.

OMA

RFEARSH.

OrthoDB

EOG46Q6RS.

Gene expression databases

ArrayExpress

Q9NZM3.

Bgee

Q9NZM3.

CleanEx

HS_ITSN2.

Genevestigator

Q9NZM3.

GermOnline

ENSG00000198399. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 2 hits.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.

InterPro

IPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR018029. C2_membr_targeting.
IPR000219. DH-domain.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EPS15_homology.
IPR027029. Intersectin-2.
IPR000108. p67phox.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

PANTHER

PTHR11216:SF29. PTHR11216:SF29. 1 hit.

Pfam

PF00168. C2. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 4 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]

PRINTS

PR00499. P67PHOX.
PR00452. SH3DOMAIN.

SMART

SM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00027. EH. 2 hits.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 5 hits.
[Graphical view]

SUPFAM

SSF49562. C2_CaLB. 1 hit.
SSF48065. DH-domain. 1 hit.
SSF50044. SH3. 5 hits.

PROSITE

PS50004. C2. 1 hit.
PS50010. DH_2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS50031. EH. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 5 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9NZM3.

GenomeRNAi

50618.

NextBio

53140.

SOURCE

Search...

Entry information

Entry name

ITSN2_HUMAN

Accession

Primary (citable) accession number: Q9NZM3
Secondary accession number(s): O95062 , Q15812, Q9HAK4, Q9NXE6, Q9NYG0, Q9NZM2, Q9ULG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 148 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.