Reviewed, UniProtKB/Swiss-Prot Q9NZM3 (ITSN2_HUMAN)

Names and origin

Protein names

Recommended name:
    Intersectin-2
Alternative name(s):
    SH3 domain-containing protein 1B
    SH3P18
    SH3P18-like WASP-associated protein

Gene names

Name:	ITSN2
Synonyms:	KIAA1256, SH3D1B, SWAP

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1696 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles.
Subunit structure	Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process.
Subcellular location	Cytoplasm.
Tissue specificity	Ubiquitous. Isoform 1 is primarily expressed in adult heart and liver.
Miscellaneous	Overexpression results in the inhibition of the transferrin uptake and the blockage of the clathrin-mediated endocytosis.
Sequence similarities	Contains 1 C2 domain. Contains 1 DH (DBL-homology) domain. Contains 2 EF-hand domains. Contains 2 EH domains. Contains 1 PH domain. Contains 5 SH3 domains.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Repeat SH3 domain
Ligand	Calcium
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro SH3/SH2 adaptor activity Ref.6 Traceable author statement. Source: ProtInc calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSM8	O95777	1	EBI-308689,EBI-347779

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9NZM3-1)

Also known as: ITSN2-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NZM3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     621-647: Missing.

Isoform 3 (identifier: Q9NZM3-3)

Also known as: ITSN2-S1;

The sequence of this isoform differs from the canonical sequence as follows:
     1234-1248: FQKRMAESGFLTEGE → WRLLLASSRGICCLS
     1249-1696: Missing.

Isoform 4 (identifier: Q9NZM3-4)

Also known as: ITSN2-S2; SH3P18;

The sequence of this isoform differs from the canonical sequence as follows:
     1192-1696: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1696

1696

Intersectin-2

PRO_0000080961

Regions

Domain

21 – 109

89

EH 1

Domain

53 – 88

36

EF-hand 1

Domain

243 – 332

90

EH 2

Domain

756 – 817

62

SH3 1

Domain

897 – 955

59

SH3 2

Domain

980 – 1038

59

SH3 3

Domain

1052 – 1116

65

SH3 4

Domain

1126 – 1185

60

SH3 5

Domain

1208 – 1394

187

DH

Domain

1433 – 1543

111

PH

Domain

1555 – 1651

97

C2

Calcium binding

66 – 78

13

Potential

Coiled coil

360 – 755

396

Potential

Amino acid modifications

Modified residue

209

1

Phosphoserine By similarity

Modified residue

552

1

Phosphotyrosine Ref.9 Ref.11

Modified residue

858

1

Phosphotyrosine Ref.10

Modified residue

883

1

Phosphoserine Ref.12 Ref.13

Modified residue

888

1

Phosphoserine Ref.12 Ref.13

Modified residue

967

1

Phosphotyrosine Ref.10 Ref.8

Natural variations

Alternative sequence

621 – 647

27

Missing in isoform 2.

VSP_003892

Alternative sequence

1192 – 1696

505

Missing in isoform 4.

VSP_003895

Alternative sequence

1234 – 1248

15

FQKRM…LTEGE → WRLLLASSRGICCLS in isoform 3.

VSP_003893

Alternative sequence

1249 – 1696

448

Missing in isoform 3.

VSP_003894

Natural variant

253

1

T → A: dbSNP rs6744320.

VAR_024287

Natural variant

290

1

V → I: dbSNP rs7603997. Ref.4

VAR_024288

Natural variant

1286

1

I → T: dbSNP rs3731625.

VAR_020193

Natural variant

1533

1

A → T: dbSNP rs2303291.

VAR_021937

Experimental info

Sequence conflict

678

1

R → G Ref.4

Sequence conflict

822 – 827

6

KAVSPK → FAAAST Ref.5

Sequence conflict

944 – 950

7

WFPKSYV → EFAAAST Ref.6

Sequence conflict

1278 – 1284

7

GEKMPVQ → VDAAANS Ref.5

Sequence conflict

1552

1

K → Q Ref.2

Sequence conflict

1552

1

K → Q Ref.3

Secondary structure

1

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1696

Helix Strand Turn

Details...

Beta strand

761 – 765

5

Beta strand

771 – 773

3

Beta strand

782 – 785

4

Beta strand

792 – 800

9

Beta strand

803 – 808

6

Beta strand

811 – 814

4

Beta strand

817 – 820

4

Beta strand

901 – 906

6

Beta strand

923 – 929

7

Beta strand

931 – 938

8

Beta strand

944 – 946

3

Helix

947 – 949

3

Beta strand

950 – 952

3

Beta strand

982 – 986

5

Beta strand

994 – 998

5

Beta strand

1006 – 1009

4

Beta strand

1011 – 1013

3

Beta strand

1016 – 1019

4

Beta strand

1026 – 1028

3

Helix

1030 – 1032

3

Beta strand

1128 – 1135

8

Beta strand

1152 – 1155

4

Beta strand

1160 – 1165

6

Beta strand

1167 – 1170

4

Beta strand

1179 – 1182

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (ITSN2-L) [UniParc]. Last modified April 3, 2002. Version 2. Checksum: 17B5C8629BBCFF9B	FASTA	1,696	193,330
	10 20 30 40 50 60 MAQFPTAMNG GPNMWAITSE ERTKHDRQFD NLKPSGGYIT GDQARNFFLQ SGLPAPVLAE 70 80 90 100 110 120 IWALSDLNKD GKMDQQEFSI AMKLIKLKLQ GQQLPVVLPP IMKQPPMFSP LISARFGMGS 130 140 150 160 170 180 MPNLSIPQPL PPAAPITSLS SATSGTNLPP LMMPTPLVPS VSTSSLPNGT ASLIQPLPIP 190 200 210 220 230 240 YSSSTLPHGS SYSLMMGGFG GASIQKAQSL IDLGSSSSTS STASLSGNSP KTGTSEWAVP 250 260 270 280 290 300 QPTRLKYRQK FNTLDKSMSG YLSGFQARNA LLQSNLSQTQ LATIWTLADV DGDGQLKAEE 310 320 330 340 350 360 FILAMHLTDM AKAGQPLPLT LPPELVPPSF RGGKQIDSIN GTLPSYQKMQ EEEPQKKLPV 370 380 390 400 410 420 TFEDKRKANY ERGNMELEKR RQALMEQQQR EAERKAQKEK EEWERKQREL QEQEWKKQLE 430 440 450 460 470 480 LEKRLEKQRE LERQREEERR KDIERREAAK QELERQRRLE WERIRRQELL NQKNREQEEI 490 500 510 520 530 540 VRLNSKKKNL HLELEALNGK HQQISGRLQD VRLKKQTQKT ELEVLDKQCD LEIMEIKQLQ 550 560 570 580 590 600 QELQEYQNKL IYLVPEKQLL NERIKNMQFS NTPDSGVSLL HKKSLEKEEL CQRLKEQLDA 610 620 630 640 650 660 LEKETASKLS EMDSFNNQLK CGNMDDSVLQ CLLSLLSCLN NLFLLLKELR ETYNTQQLAL 670 680 690 700 710 720 EQLYKIKRDK LKEIERKRLE LMQKKKLEDE AARKAKQGKE NLWKENLRKE EEEKQKRLQE 730 740 750 760 770 780 EKTQEKIQEE ERKAEEKQRK DKDTLKAEEK KRETASVLVN YRALYPFEAR NHDEMSFNSG 790 800 810 820 830 840 DIIQVDEKTV GEPGWLYGSF QGNFGWFPCN YVEKMPSSEN EKAVSPKKAL LPPTVSLSAT 850 860 870 880 890 900 STSSEPLSSN QPASVTDYQN VSFSNLTVNT SWQKKSAFTR TVSPGSVSPI HGQGQVVENL 910 920 930 940 950 960 KAQALCSWTA KKDNHLNFSK HDIITVLEQQ ENWWFGEVHG GRGWFPKSYV KIIPGSEVKR 970 980 990 1000 1010 1020 EEPEALYAAV NKKPTSAAYS VGEEYIALYP YSSVEPGDLT FTEGEEILVT QKDGEWWTGS 1030 1040 1050 1060 1070 1080 IGDRSGIFPS NYVKPKDQES FGSASKSGAS NKKPEIAQVT SAYVASGSEQ LSLAPGQLIL 1090 1100 1110 1120 1130 1140 ILKKNTSGWW QGELQARGKK RQKGWFPASH VKLLGPSSER ATPAFHPVCQ VIAMYDYAAN 1150 1160 1170 1180 1190 1200 NEDELSFSKG QLINVMNKDD PDWWQGEING VTGLFPSNYV KMTTDSDPSQ QWCADLQTLD 1210 1220 1230 1240 1250 1260 TMQPIERKRQ GYIHELIQTE ERYMADLQLV VEVFQKRMAE SGFLTEGEMA LIFVNWKELI 1270 1280 1290 1300 1310 1320 MSNTKLLKAL RVRKKTGGEK MPVQMIGDIL AAELSHMQAY IRFCSCQLNG AALLQQKTDE 1330 1340 1350 1360 1370 1380 DTDFKEFLKK LASDPRCKGM PLSSFLLKPM QRITRYPLLI RSILENTPES HADHSSLKLA 1390 1400 1410 1420 1430 1440 LERAEELCSQ VNEGVREKEN SDRLEWIQAH VQCEGLAEQL IFNSLTNCLG PRKLLHSGKL 1450 1460 1470 1480 1490 1500 YKTKSNKELH GFLFNDFLLL TYMVKQFAVS SGSEKLFSSK SNAQFKMYKT PIFLNEVLVK 1510 1520 1530 1540 1550 1560 LPTDPSSDEP VFHISHIDRV YTLRTDNINE RTAWVQKIKA ASEQYIDTEK KKREKAYQAR 1570 1580 1590 1600 1610 1620 SQKTSGIGRL MVHVIEATEL KACKPNGKSN PYCEISMGSQ SYTTRTIQDT LNPKWNFNCQ 1630 1640 1650 1660 1670 1680 FFIKDLYQDV LCLTLFDRDQ FSPDDFLGRT EIPVAKIRTE QESKGPMTRR LLLHEVPTGE 1690 VWVRFDLQLF EQKTLL
	Isoform 2. Checksum: DB55C4E85BAE5EF1	FASTA	1,669	190,365
	10 20 30 40 50 60 MAQFPTAMNG GPNMWAITSE ERTKHDRQFD NLKPSGGYIT GDQARNFFLQ SGLPAPVLAE 70 80 90 100 110 120 IWALSDLNKD GKMDQQEFSI AMKLIKLKLQ GQQLPVVLPP IMKQPPMFSP LISARFGMGS 130 140 150 160 170 180 MPNLSIPQPL PPAAPITSLS SATSGTNLPP LMMPTPLVPS VSTSSLPNGT ASLIQPLPIP 190 200 210 220 230 240 YSSSTLPHGS SYSLMMGGFG GASIQKAQSL IDLGSSSSTS STASLSGNSP KTGTSEWAVP 250 260 270 280 290 300 QPTRLKYRQK FNTLDKSMSG YLSGFQARNA LLQSNLSQTQ LATIWTLADV DGDGQLKAEE 310 320 330 340 350 360 FILAMHLTDM AKAGQPLPLT LPPELVPPSF RGGKQIDSIN GTLPSYQKMQ EEEPQKKLPV 370 380 390 400 410 420 TFEDKRKANY ERGNMELEKR RQALMEQQQR EAERKAQKEK EEWERKQREL QEQEWKKQLE 430 440 450 460 470 480 LEKRLEKQRE LERQREEERR KDIERREAAK QELERQRRLE WERIRRQELL NQKNREQEEI 490 500 510 520 530 540 VRLNSKKKNL HLELEALNGK HQQISGRLQD VRLKKQTQKT ELEVLDKQCD LEIMEIKQLQ 550 560 570 580 590 600 QELQEYQNKL IYLVPEKQLL NERIKNMQFS NTPDSGVSLL HKKSLEKEEL CQRLKEQLDA 610 620 630 640 650 660 LEKETASKLS EMDSFNNQLK ELRETYNTQQ LALEQLYKIK RDKLKEIERK RLELMQKKKL 670 680 690 700 710 720 EDEAARKAKQ GKENLWKENL RKEEEEKQKR LQEEKTQEKI QEEERKAEEK QRKDKDTLKA 730 740 750 760 770 780 EEKKRETASV LVNYRALYPF EARNHDEMSF NSGDIIQVDE KTVGEPGWLY GSFQGNFGWF 790 800 810 820 830 840 PCNYVEKMPS SENEKAVSPK KALLPPTVSL SATSTSSEPL SSNQPASVTD YQNVSFSNLT 850 860 870 880 890 900 VNTSWQKKSA FTRTVSPGSV SPIHGQGQVV ENLKAQALCS WTAKKDNHLN FSKHDIITVL 910 920 930 940 950 960 EQQENWWFGE VHGGRGWFPK SYVKIIPGSE VKREEPEALY AAVNKKPTSA AYSVGEEYIA 970 980 990 1000 1010 1020 LYPYSSVEPG DLTFTEGEEI LVTQKDGEWW TGSIGDRSGI FPSNYVKPKD QESFGSASKS 1030 1040 1050 1060 1070 1080 GASNKKPEIA QVTSAYVASG SEQLSLAPGQ LILILKKNTS GWWQGELQAR GKKRQKGWFP 1090 1100 1110 1120 1130 1140 ASHVKLLGPS SERATPAFHP VCQVIAMYDY AANNEDELSF SKGQLINVMN KDDPDWWQGE 1150 1160 1170 1180 1190 1200 INGVTGLFPS NYVKMTTDSD PSQQWCADLQ TLDTMQPIER KRQGYIHELI QTEERYMADL 1210 1220 1230 1240 1250 1260 QLVVEVFQKR MAESGFLTEG EMALIFVNWK ELIMSNTKLL KALRVRKKTG GEKMPVQMIG 1270 1280 1290 1300 1310 1320 DILAAELSHM QAYIRFCSCQ LNGAALLQQK TDEDTDFKEF LKKLASDPRC KGMPLSSFLL 1330 1340 1350 1360 1370 1380 KPMQRITRYP LLIRSILENT PESHADHSSL KLALERAEEL CSQVNEGVRE KENSDRLEWI 1390 1400 1410 1420 1430 1440 QAHVQCEGLA EQLIFNSLTN CLGPRKLLHS GKLYKTKSNK ELHGFLFNDF LLLTYMVKQF 1450 1460 1470 1480 1490 1500 AVSSGSEKLF SSKSNAQFKM YKTPIFLNEV LVKLPTDPSS DEPVFHISHI DRVYTLRTDN 1510 1520 1530 1540 1550 1560 INERTAWVQK IKAASEQYID TEKKKREKAY QARSQKTSGI GRLMVHVIEA TELKACKPNG 1570 1580 1590 1600 1610 1620 KSNPYCEISM GSQSYTTRTI QDTLNPKWNF NCQFFIKDLY QDVLCLTLFD RDQFSPDDFL 1630 1640 1650 1660 GRTEIPVAKI RTEQESKGPM TRRLLLHEVP TGEVWVRFDL QLFEQKTLL
	Isoform 3 (ITSN2-S1). Checksum: A325BC186C76A97D	FASTA	1,248	141,719
	10 20 30 40 50 60 MAQFPTAMNG GPNMWAITSE ERTKHDRQFD NLKPSGGYIT GDQARNFFLQ SGLPAPVLAE 70 80 90 100 110 120 IWALSDLNKD GKMDQQEFSI AMKLIKLKLQ GQQLPVVLPP IMKQPPMFSP LISARFGMGS 130 140 150 160 170 180 MPNLSIPQPL PPAAPITSLS SATSGTNLPP LMMPTPLVPS VSTSSLPNGT ASLIQPLPIP 190 200 210 220 230 240 YSSSTLPHGS SYSLMMGGFG GASIQKAQSL IDLGSSSSTS STASLSGNSP KTGTSEWAVP 250 260 270 280 290 300 QPTRLKYRQK FNTLDKSMSG YLSGFQARNA LLQSNLSQTQ LATIWTLADV DGDGQLKAEE 310 320 330 340 350 360 FILAMHLTDM AKAGQPLPLT LPPELVPPSF RGGKQIDSIN GTLPSYQKMQ EEEPQKKLPV 370 380 390 400 410 420 TFEDKRKANY ERGNMELEKR RQALMEQQQR EAERKAQKEK EEWERKQREL QEQEWKKQLE 430 440 450 460 470 480 LEKRLEKQRE LERQREEERR KDIERREAAK QELERQRRLE WERIRRQELL NQKNREQEEI 490 500 510 520 530 540 VRLNSKKKNL HLELEALNGK HQQISGRLQD VRLKKQTQKT ELEVLDKQCD LEIMEIKQLQ 550 560 570 580 590 600 QELQEYQNKL IYLVPEKQLL NERIKNMQFS NTPDSGVSLL HKKSLEKEEL CQRLKEQLDA 610 620 630 640 650 660 LEKETASKLS EMDSFNNQLK CGNMDDSVLQ CLLSLLSCLN NLFLLLKELR ETYNTQQLAL 670 680 690 700 710 720 EQLYKIKRDK LKEIERKRLE LMQKKKLEDE AARKAKQGKE NLWKENLRKE EEEKQKRLQE 730 740 750 760 770 780 EKTQEKIQEE ERKAEEKQRK DKDTLKAEEK KRETASVLVN YRALYPFEAR NHDEMSFNSG 790 800 810 820 830 840 DIIQVDEKTV GEPGWLYGSF QGNFGWFPCN YVEKMPSSEN EKAVSPKKAL LPPTVSLSAT 850 860 870 880 890 900 STSSEPLSSN QPASVTDYQN VSFSNLTVNT SWQKKSAFTR TVSPGSVSPI HGQGQVVENL 910 920 930 940 950 960 KAQALCSWTA KKDNHLNFSK HDIITVLEQQ ENWWFGEVHG GRGWFPKSYV KIIPGSEVKR 970 980 990 1000 1010 1020 EEPEALYAAV NKKPTSAAYS VGEEYIALYP YSSVEPGDLT FTEGEEILVT QKDGEWWTGS 1030 1040 1050 1060 1070 1080 IGDRSGIFPS NYVKPKDQES FGSASKSGAS NKKPEIAQVT SAYVASGSEQ LSLAPGQLIL 1090 1100 1110 1120 1130 1140 ILKKNTSGWW QGELQARGKK RQKGWFPASH VKLLGPSSER ATPAFHPVCQ VIAMYDYAAN 1150 1160 1170 1180 1190 1200 NEDELSFSKG QLINVMNKDD PDWWQGEING VTGLFPSNYV KMTTDSDPSQ QWCADLQTLD 1210 1220 1230 1240 TMQPIERKRQ GYIHELIQTE ERYMADLQLV VEVWRLLLAS SRGICCLS
	Isoform 4 (ITSN2-S2) (SH3P18). Checksum: 1334FA0B1BF2BB3F	FASTA	1,191	135,013
	10 20 30 40 50 60 MAQFPTAMNG GPNMWAITSE ERTKHDRQFD NLKPSGGYIT GDQARNFFLQ SGLPAPVLAE 70 80 90 100 110 120 IWALSDLNKD GKMDQQEFSI AMKLIKLKLQ GQQLPVVLPP IMKQPPMFSP LISARFGMGS 130 140 150 160 170 180 MPNLSIPQPL PPAAPITSLS SATSGTNLPP LMMPTPLVPS VSTSSLPNGT ASLIQPLPIP 190 200 210 220 230 240 YSSSTLPHGS SYSLMMGGFG GASIQKAQSL IDLGSSSSTS STASLSGNSP KTGTSEWAVP 250 260 270 280 290 300 QPTRLKYRQK FNTLDKSMSG YLSGFQARNA LLQSNLSQTQ LATIWTLADV DGDGQLKAEE 310 320 330 340 350 360 FILAMHLTDM AKAGQPLPLT LPPELVPPSF RGGKQIDSIN GTLPSYQKMQ EEEPQKKLPV 370 380 390 400 410 420 TFEDKRKANY ERGNMELEKR RQALMEQQQR EAERKAQKEK EEWERKQREL QEQEWKKQLE 430 440 450 460 470 480 LEKRLEKQRE LERQREEERR KDIERREAAK QELERQRRLE WERIRRQELL NQKNREQEEI 490 500 510 520 530 540 VRLNSKKKNL HLELEALNGK HQQISGRLQD VRLKKQTQKT ELEVLDKQCD LEIMEIKQLQ 550 560 570 580 590 600 QELQEYQNKL IYLVPEKQLL NERIKNMQFS NTPDSGVSLL HKKSLEKEEL CQRLKEQLDA 610 620 630 640 650 660 LEKETASKLS EMDSFNNQLK CGNMDDSVLQ CLLSLLSCLN NLFLLLKELR ETYNTQQLAL 670 680 690 700 710 720 EQLYKIKRDK LKEIERKRLE LMQKKKLEDE AARKAKQGKE NLWKENLRKE EEEKQKRLQE 730 740 750 760 770 780 EKTQEKIQEE ERKAEEKQRK DKDTLKAEEK KRETASVLVN YRALYPFEAR NHDEMSFNSG 790 800 810 820 830 840 DIIQVDEKTV GEPGWLYGSF QGNFGWFPCN YVEKMPSSEN EKAVSPKKAL LPPTVSLSAT 850 860 870 880 890 900 STSSEPLSSN QPASVTDYQN VSFSNLTVNT SWQKKSAFTR TVSPGSVSPI HGQGQVVENL 910 920 930 940 950 960 KAQALCSWTA KKDNHLNFSK HDIITVLEQQ ENWWFGEVHG GRGWFPKSYV KIIPGSEVKR 970 980 990 1000 1010 1020 EEPEALYAAV NKKPTSAAYS VGEEYIALYP YSSVEPGDLT FTEGEEILVT QKDGEWWTGS 1030 1040 1050 1060 1070 1080 IGDRSGIFPS NYVKPKDQES FGSASKSGAS NKKPEIAQVT SAYVASGSEQ LSLAPGQLIL 1090 1100 1110 1120 1130 1140 ILKKNTSGWW QGELQARGKK RQKGWFPASH VKLLGPSSER ATPAFHPVCQ VIAMYDYAAN 1150 1160 1170 1180 1190 NEDELSFSKG QLINVMNKDD PDWWQGEING VTGLFPSNYV KMTTDSDPSQ Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis." Pucharcos C., Estivill X., de la Luna S. FEBS Lett. 478:43-51(2000) [PubMed: 10922467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3). Tissue: Fetal brain and Fetal liver.
[2]	"Intersectin 2 (SH3D1B), human homolog of mouse Ese2 protein." Seifert M., Engel M., Welter C. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O. DNA Res. 6:337-345(1999) [PubMed: 10574462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 258-720 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1261-1696, VARIANT ILE-290. Tissue: Embryo.
[5]	"SH3P18-like wasp associated protein (SWAP): a multiple SH3 domain containing protein that interacts with WASP." Wilson L.A., Fields D., Cruz L., Friesen J., Siminovitch K.A. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 821-1284 (ISOFORM 1).
[6]	"Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins." Sparks A.B., Hoffman N.G., McConnell S.J., Fowlkes D.M., Kay B.K. Nat. Biotechnol. 14:741-744(1996) [PubMed: 9630982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 944-1191 (ISOFORM 4). Tissue: Bone marrow.
[7]	"The human intersectin genes and their spliced variants are differentially expressed." Pucharcos C., Casas C., Nadal M., Estivill X., de la Luna S. Biochim. Biophys. Acta 1521:1-11(2001) [PubMed: 11690630] [Abstract] Cited for: ALTERNATIVE SPLICING. Tissue: Brain and Fetal liver.
[8]	"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-967, MASS SPECTROMETRY. Tissue: T-cell.
[9]	"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J., Lauffenburger D.A., White F.M. Mol. Cell. Proteomics 4:1240-1250(2005) [PubMed: 15951569] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-552, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-858 AND TYR-967, MASS SPECTROMETRY.
[11]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-552, MASS SPECTROMETRY.
[12]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-883 AND SER-888, MASS SPECTROMETRY.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-883 AND SER-888, MASS SPECTROMETRY.
[14]	"Solution structure of SH3 domains of human intersectin 2 (KIAA1256)." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 761-1186.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF182198 mRNA. Translation: AAF59903.1.
AF182199 mRNA. Translation: AAF59904.1.
AF248540 mRNA. Translation: AAF63600.1. Different initiation.
AB033082 mRNA. Translation: BAA86570.1. Different initiation.
AK021545 mRNA. Translation: BAB13841.1.
AK000302 mRNA. Translation: BAA91068.1.
AF001630 mRNA. Translation: AAD00899.1.
U61167 mRNA. Translation: AAC50593.1.

IPI

IPI00216423.
IPI00396534.
IPI00414027.
IPI00892957.

RefSeq

NP_006268.2.
NP_062541.3.
NP_671494.2.

UniGene

Hs.432562

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J3T	NMR	-	A	897-957	[»]
1UDL	NMR	-	A	1102-1186	[»]
1UE9	NMR	-	A	1055-1121	[»]
1UFF	NMR	-	A	761-841	[»]
1UHF	NMR	-	A	982-1037	[»]
3GF9	X-ray	2.50	A	1129-1405	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9NZM3. 9 interactions.

PTM databases

PhosphoSite

Q9NZM3.

Proteomic databases

PRIDE

Q9NZM3.

Genome annotation databases

Ensembl

ENSG00000198399. Homo sapiens. [Contig view]

GeneID

50618.

KEGG

hsa:50618.

UCSC

uc002rfe.1. human.
uc002rff.1. human.
uc002rfg.1. human.

Organism-specific databases

GeneCards

GC02M024337.

H-InvDB

HIX0001876.

HGNC

HGNC:6184. ITSN2.

MIM

604464. gene.

PharmGKB

PA29982.

HUGE

Search...

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q9NZM3.

OMA

Q9NZM3. LTVNTSW.

Gene expression databases

ArrayExpress

Q9NZM3.

Bgee

Q9NZM3.

CleanEx

HS_ITSN2.

GermOnline

ENSG00000198399. Homo sapiens.

Family and domain databases

InterPro

IPR000008. C2_Ca-dep.
IPR018029. C2_membr_targeting.
IPR000219. DH-domain.
IPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
IPR000261. EPS15_homology.
IPR000108. Neu_cyt_fact_2.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 2 hits.
G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:1.20.900.10. RhoGEF. 1 hit.

Pfam

PF00168. C2. 1 hit.
PF00036. efhand. 2 hits.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 4 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]

PRINTS

PR00499. P67PHOX.

ProDom

PD000012. EF-hand. 1 hit.
PD000066. SH3. 5 hits.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00027. EH. 2 hits.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 5 hits.
[Graphical view]

PROSITE

PS50004. C2. 1 hit.
PS50010. DH_2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS50031. EH. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 5 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

53140.

SOURCE

Search...

Entry information

Entry name

ITSN2_HUMAN

Accession

Primary (citable) accession number: Q9NZM3
Secondary accession number(s): O95062 , Q15812, Q9HAK4, Q9NXE6, Q9NYG0, Q9NZM2, Q9ULG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	April 3, 2002
Last modified:	July 7, 2009
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)