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Q9NZM3

- ITSN2_HUMAN

UniProt

Q9NZM3 - ITSN2_HUMAN

Protein

Intersectin-2

Gene

ITSN2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 162 (01 Oct 2014)
      Sequence version 3 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR).2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi67 – 7913PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. protein binding Source: IntAct
    3. Rho guanyl-nucleotide exchange factor activity Source: InterPro
    4. SH3/SH2 adaptor activity Source: ProtInc

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. positive regulation of signal transduction Source: GOC

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intersectin-2
    Alternative name(s):
    SH3 domain-containing protein 1B
    SH3P18
    SH3P18-like WASP-associated protein
    Gene namesi
    Name:ITSN2
    Synonyms:KIAA1256, SH3D1B, SWAP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:6184. ITSN2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29982.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16971697Intersectin-2PRO_0000080961Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei210 – 2101Phosphoserine1 Publication
    Modified residuei230 – 2301Phosphoserine1 Publication
    Modified residuei553 – 5531PhosphotyrosineBy similarity
    Modified residuei573 – 5731Phosphothreonine1 Publication
    Modified residuei884 – 8841PhosphoserineBy similarity
    Modified residuei889 – 8891Phosphoserine3 Publications
    Modified residuei968 – 9681Phosphotyrosine4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9NZM3.
    PaxDbiQ9NZM3.
    PRIDEiQ9NZM3.

    PTM databases

    PhosphoSiteiQ9NZM3.

    Expressioni

    Tissue specificityi

    Ubiquitous. Isoform 1 is primarily expressed in adult heart and liver.

    Gene expression databases

    ArrayExpressiQ9NZM3.
    BgeeiQ9NZM3.
    CleanExiHS_ITSN2.
    GenevestigatoriQ9NZM3.

    Organism-specific databases

    HPAiHPA036475.

    Interactioni

    Subunit structurei

    Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process. Interacts with ADAM15. Interacts with FASLG. Interacts with ANKRD54 By similarity. Interacts with FCHO2.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    K15-MQ9WNA92EBI-308689,EBI-7555439From a different organism.

    Protein-protein interaction databases

    BioGridi119098. 79 interactions.
    IntActiQ9NZM3. 24 interactions.
    MINTiMINT-1404321.
    STRINGi9606.ENSP00000347244.

    Structurei

    Secondary structure

    1
    1697
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi762 – 7665
    Beta strandi772 – 7743
    Beta strandi783 – 7864
    Beta strandi793 – 8019
    Beta strandi804 – 8096
    Beta strandi812 – 8154
    Beta strandi818 – 8214
    Beta strandi901 – 9077
    Beta strandi924 – 9318
    Beta strandi934 – 9396
    Beta strandi942 – 9476
    Helixi948 – 9503
    Beta strandi951 – 9544
    Beta strandi983 – 9875
    Beta strandi995 – 9995
    Beta strandi1007 – 10104
    Beta strandi1012 – 10143
    Beta strandi1017 – 10204
    Beta strandi1027 – 10293
    Helixi1031 – 10333
    Beta strandi1056 – 10605
    Beta strandi1079 – 10857
    Beta strandi1087 – 10959
    Beta strandi1104 – 11085
    Beta strandi1111 – 11144
    Beta strandi1129 – 11368
    Beta strandi1153 – 11564
    Beta strandi1161 – 11666
    Beta strandi1168 – 11714
    Beta strandi1180 – 11834
    Helixi1207 – 123428
    Helixi1236 – 12427
    Beta strandi1243 – 12453
    Helixi1247 – 12548
    Helixi1257 – 127216
    Helixi1288 – 12947
    Helixi1299 – 132123
    Helixi1323 – 133311
    Turni1336 – 13405
    Helixi1343 – 13464
    Helixi1349 – 136618
    Helixi1375 – 139319
    Beta strandi1569 – 158012
    Beta strandi1592 – 15987
    Beta strandi1601 – 16044
    Beta strandi1615 – 162511
    Turni1627 – 16293
    Beta strandi1631 – 16388
    Beta strandi1641 – 16444
    Beta strandi1647 – 16548
    Helixi1655 – 166511
    Beta strandi1670 – 16734
    Beta strandi1675 – 16795
    Beta strandi1681 – 169010

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J3TNMR-A898-958[»]
    1UDLNMR-A1103-1187[»]
    1UE9NMR-A1056-1122[»]
    1UFFNMR-A762-842[»]
    1UHFNMR-A983-1038[»]
    3GF9X-ray2.50A1130-1406[»]
    3JZYX-ray1.56A1201-1692[»]
    4IIOX-ray1.70A/B901-955[»]
    ProteinModelPortaliQ9NZM3.
    SMRiQ9NZM3. Positions 2-108, 236-332, 762-845, 901-1551, 1568-1691.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9NZM3.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 11089EH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini54 – 8936EF-handPROSITE-ProRule annotationAdd
    BLAST
    Domaini244 – 33390EH 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini757 – 81862SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini898 – 95659SH3 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini981 – 103959SH3 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini1053 – 111765SH3 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini1127 – 118660SH3 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini1209 – 1395187DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini1434 – 1544111PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini1556 – 165297C2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili361 – 756396Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 2 EH domains.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 5 SH3 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiCOG5422.
    HOVERGENiHBG052159.
    InParanoidiQ9NZM3.
    OMAiWQQKSAF.
    OrthoDBiEOG7WMCJ3.
    PhylomeDBiQ9NZM3.
    TreeFamiTF324293.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    InterProiIPR000008. C2_dom.
    IPR000219. DH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027029. Intersectin-2.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR11216:SF29. PTHR11216:SF29. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF00621. RhoGEF. 1 hit.
    PF00018. SH3_1. 2 hits.
    PF07653. SH3_2. 1 hit.
    PF14604. SH3_9. 2 hits.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00027. EH. 2 hits.
    SM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    SM00326. SH3. 5 hits.
    [Graphical view]
    SUPFAMiSSF48065. SSF48065. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF50044. SSF50044. 5 hits.
    PROSITEiPS50004. C2. 1 hit.
    PS50010. DH_2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS50031. EH. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50002. SH3. 5 hits.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q9NZM3-1) [UniParc]FASTAAdd to Basket

    Also known as: ITSN2-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMAQFPTAMN GGPNMWAITS EERTKHDRQF DNLKPSGGYI TGDQARNFFL     50
    QSGLPAPVLA EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP 100
    PIMKQPPMFS PLISARFGMG SMPNLSIPQP LPPAAPITSL SSATSGTNLP 150
    PLMMPTPLVP SVSTSSLPNG TASLIQPLPI PYSSSTLPHG SSYSLMMGGF 200
    GGASIQKAQS LIDLGSSSST SSTASLSGNS PKTGTSEWAV PQPTRLKYRQ 250
    KFNTLDKSMS GYLSGFQARN ALLQSNLSQT QLATIWTLAD VDGDGQLKAE 300
    EFILAMHLTD MAKAGQPLPL TLPPELVPPS FRGGKQIDSI NGTLPSYQKM 350
    QEEEPQKKLP VTFEDKRKAN YERGNMELEK RRQALMEQQQ REAERKAQKE 400
    KEEWERKQRE LQEQEWKKQL ELEKRLEKQR ELERQREEER RKDIERREAA 450
    KQELERQRRL EWERIRRQEL LNQKNREQEE IVRLNSKKKN LHLELEALNG 500
    KHQQISGRLQ DVRLKKQTQK TELEVLDKQC DLEIMEIKQL QQELQEYQNK 550
    LIYLVPEKQL LNERIKNMQF SNTPDSGVSL LHKKSLEKEE LCQRLKEQLD 600
    ALEKETASKL SEMDSFNNQL KCGNMDDSVL QCLLSLLSCL NNLFLLLKEL 650
    RETYNTQQLA LEQLYKIKRD KLKEIERKRL ELMQKKKLED EAARKAKQGK 700
    ENLWKENLRK EEEEKQKRLQ EEKTQEKIQE EERKAEEKQR KDKDTLKAEE 750
    KKRETASVLV NYRALYPFEA RNHDEMSFNS GDIIQVDEKT VGEPGWLYGS 800
    FQGNFGWFPC NYVEKMPSSE NEKAVSPKKA LLPPTVSLSA TSTSSEPLSS 850
    NQPASVTDYQ NVSFSNLTVN TSWQKKSAFT RTVSPGSVSP IHGQGQVVEN 900
    LKAQALCSWT AKKDNHLNFS KHDIITVLEQ QENWWFGEVH GGRGWFPKSY 950
    VKIIPGSEVK REEPEALYAA VNKKPTSAAY SVGEEYIALY PYSSVEPGDL 1000
    TFTEGEEILV TQKDGEWWTG SIGDRSGIFP SNYVKPKDQE SFGSASKSGA 1050
    SNKKPEIAQV TSAYVASGSE QLSLAPGQLI LILKKNTSGW WQGELQARGK 1100
    KRQKGWFPAS HVKLLGPSSE RATPAFHPVC QVIAMYDYAA NNEDELSFSK 1150
    GQLINVMNKD DPDWWQGEIN GVTGLFPSNY VKMTTDSDPS QQWCADLQTL 1200
    DTMQPIERKR QGYIHELIQT EERYMADLQL VVEVFQKRMA ESGFLTEGEM 1250
    ALIFVNWKEL IMSNTKLLKA LRVRKKTGGE KMPVQMIGDI LAAELSHMQA 1300
    YIRFCSCQLN GAALLQQKTD EDTDFKEFLK KLASDPRCKG MPLSSFLLKP 1350
    MQRITRYPLL IRSILENTPE SHADHSSLKL ALERAEELCS QVNEGVREKE 1400
    NSDRLEWIQA HVQCEGLAEQ LIFNSLTNCL GPRKLLHSGK LYKTKSNKEL 1450
    HGFLFNDFLL LTYMVKQFAV SSGSEKLFSS KSNAQFKMYK TPIFLNEVLV 1500
    KLPTDPSSDE PVFHISHIDR VYTLRTDNIN ERTAWVQKIK AASEQYIDTE 1550
    KKKREKAYQA RSQKTSGIGR LMVHVIEATE LKACKPNGKS NPYCEISMGS 1600
    QSYTTRTIQD TLNPKWNFNC QFFIKDLYQD VLCLTLFDRD QFSPDDFLGR 1650
    TEIPVAKIRT EQESKGPMTR RLLLHEVPTG EVWVRFDLQL FEQKTLL 1697
    Length:1,697
    Mass (Da):193,461
    Last modified:April 20, 2010 - v3
    Checksum:i49C41AE1E32E1BD0
    GO
    Isoform 2 (identifier: Q9NZM3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         622-648: Missing.

    Show »
    Length:1,670
    Mass (Da):190,496
    Checksum:i91E65C08E5AB26F1
    GO
    Isoform 3 (identifier: Q9NZM3-3) [UniParc]FASTAAdd to Basket

    Also known as: ITSN2-S1

    The sequence of this isoform differs from the canonical sequence as follows:
         1235-1249: FQKRMAESGFLTEGE → RRLLLASSRGICCLS
         1250-1697: Missing.

    Show »
    Length:1,249
    Mass (Da):141,820
    Checksum:i271797AF71A0EB28
    GO
    Isoform 4 (identifier: Q9NZM3-4) [UniParc]FASTAAdd to Basket

    Also known as: ITSN2-S2, SH3P18

    The sequence of this isoform differs from the canonical sequence as follows:
         1193-1697: Missing.

    Show »
    Length:1,192
    Mass (Da):135,144
    Checksum:i2DA2049587CF4082
    GO

    Sequence cautioni

    The sequence AAF59903.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAF59904.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAF63600.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence BAA86570.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti679 – 6791R → G in BAB13841. (PubMed:14702039)Curated
    Sequence conflicti823 – 8286KAVSPK → FAAAST in AAD00899. 1 PublicationCurated
    Sequence conflicti945 – 9517WFPKSYV → EFAAAST in AAC50593. (PubMed:9630982)Curated
    Sequence conflicti1279 – 12857GEKMPVQ → VDAAANS in AAD00899. 1 PublicationCurated
    Sequence conflicti1553 – 15531K → Q in AAF63600. 1 PublicationCurated
    Sequence conflicti1553 – 15531K → Q in BAA86570. (PubMed:10574462)Curated
    Isoform 3 (identifier: Q9NZM3-3)
    Sequence conflicti1235 – 12351R → W in AAF59904. (PubMed:10922467)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti254 – 2541T → A.
    Corresponds to variant rs6744320 [ dbSNP | Ensembl ].
    VAR_024287
    Natural varianti291 – 2911V → I.1 Publication
    Corresponds to variant rs7603997 [ dbSNP | Ensembl ].
    VAR_024288
    Natural varianti1287 – 12871I → T.
    Corresponds to variant rs3731625 [ dbSNP | Ensembl ].
    VAR_020193
    Natural varianti1534 – 15341A → T.
    Corresponds to variant rs2303291 [ dbSNP | Ensembl ].
    VAR_021937

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei622 – 64827Missing in isoform 2. 3 PublicationsVSP_003892Add
    BLAST
    Alternative sequencei1193 – 1697505Missing in isoform 4. 1 PublicationVSP_003895Add
    BLAST
    Alternative sequencei1235 – 124915FQKRM…LTEGE → RRLLLASSRGICCLS in isoform 3. 1 PublicationVSP_003893Add
    BLAST
    Alternative sequencei1250 – 1697448Missing in isoform 3. 1 PublicationVSP_003894Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF182198 mRNA. Translation: AAF59903.1. Different initiation.
    AF182199 mRNA. Translation: AAF59904.1. Different initiation.
    AF248540 mRNA. Translation: AAF63600.1. Different initiation.
    AB033082 mRNA. Translation: BAA86570.1. Different initiation.
    AC008073 Genomic DNA. No translation available.
    AC009228 Genomic DNA. No translation available.
    AK021545 mRNA. Translation: BAB13841.1.
    AK000302 mRNA. Translation: BAA91068.1.
    AF001630 mRNA. Translation: AAD00899.1.
    U61167 mRNA. Translation: AAC50593.1.
    CCDSiCCDS1710.2. [Q9NZM3-1]
    CCDS1711.2. [Q9NZM3-2]
    CCDS46230.1. [Q9NZM3-3]
    RefSeqiNP_006268.2. NM_006277.2. [Q9NZM3-1]
    NP_062541.3. NM_019595.3. [Q9NZM3-2]
    NP_671494.2. NM_147152.2. [Q9NZM3-3]
    UniGeneiHs.432562.

    Genome annotation databases

    EnsembliENST00000355123; ENSP00000347244; ENSG00000198399. [Q9NZM3-1]
    ENST00000361999; ENSP00000354561; ENSG00000198399. [Q9NZM3-2]
    ENST00000406921; ENSP00000384499; ENSG00000198399. [Q9NZM3-3]
    GeneIDi50618.
    KEGGihsa:50618.
    UCSCiuc002rfe.2. human. [Q9NZM3-1]
    uc002rff.2. human. [Q9NZM3-2]
    uc002rfg.3. human. [Q9NZM3-3]

    Polymorphism databases

    DMDMi294862505.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF182198 mRNA. Translation: AAF59903.1 . Different initiation.
    AF182199 mRNA. Translation: AAF59904.1 . Different initiation.
    AF248540 mRNA. Translation: AAF63600.1 . Different initiation.
    AB033082 mRNA. Translation: BAA86570.1 . Different initiation.
    AC008073 Genomic DNA. No translation available.
    AC009228 Genomic DNA. No translation available.
    AK021545 mRNA. Translation: BAB13841.1 .
    AK000302 mRNA. Translation: BAA91068.1 .
    AF001630 mRNA. Translation: AAD00899.1 .
    U61167 mRNA. Translation: AAC50593.1 .
    CCDSi CCDS1710.2. [Q9NZM3-1 ]
    CCDS1711.2. [Q9NZM3-2 ]
    CCDS46230.1. [Q9NZM3-3 ]
    RefSeqi NP_006268.2. NM_006277.2. [Q9NZM3-1 ]
    NP_062541.3. NM_019595.3. [Q9NZM3-2 ]
    NP_671494.2. NM_147152.2. [Q9NZM3-3 ]
    UniGenei Hs.432562.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J3T NMR - A 898-958 [» ]
    1UDL NMR - A 1103-1187 [» ]
    1UE9 NMR - A 1056-1122 [» ]
    1UFF NMR - A 762-842 [» ]
    1UHF NMR - A 983-1038 [» ]
    3GF9 X-ray 2.50 A 1130-1406 [» ]
    3JZY X-ray 1.56 A 1201-1692 [» ]
    4IIO X-ray 1.70 A/B 901-955 [» ]
    ProteinModelPortali Q9NZM3.
    SMRi Q9NZM3. Positions 2-108, 236-332, 762-845, 901-1551, 1568-1691.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119098. 79 interactions.
    IntActi Q9NZM3. 24 interactions.
    MINTi MINT-1404321.
    STRINGi 9606.ENSP00000347244.

    PTM databases

    PhosphoSitei Q9NZM3.

    Polymorphism databases

    DMDMi 294862505.

    Proteomic databases

    MaxQBi Q9NZM3.
    PaxDbi Q9NZM3.
    PRIDEi Q9NZM3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000355123 ; ENSP00000347244 ; ENSG00000198399 . [Q9NZM3-1 ]
    ENST00000361999 ; ENSP00000354561 ; ENSG00000198399 . [Q9NZM3-2 ]
    ENST00000406921 ; ENSP00000384499 ; ENSG00000198399 . [Q9NZM3-3 ]
    GeneIDi 50618.
    KEGGi hsa:50618.
    UCSCi uc002rfe.2. human. [Q9NZM3-1 ]
    uc002rff.2. human. [Q9NZM3-2 ]
    uc002rfg.3. human. [Q9NZM3-3 ]

    Organism-specific databases

    CTDi 50618.
    GeneCardsi GC02M024425.
    H-InvDB HIX0001876.
    HGNCi HGNC:6184. ITSN2.
    HPAi HPA036475.
    MIMi 604464. gene.
    neXtProti NX_Q9NZM3.
    PharmGKBi PA29982.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5422.
    HOVERGENi HBG052159.
    InParanoidi Q9NZM3.
    OMAi WQQKSAF.
    OrthoDBi EOG7WMCJ3.
    PhylomeDBi Q9NZM3.
    TreeFami TF324293.

    Miscellaneous databases

    EvolutionaryTracei Q9NZM3.
    GeneWikii ITSN2.
    GenomeRNAii 50618.
    NextBioi 53140.
    PROi Q9NZM3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NZM3.
    Bgeei Q9NZM3.
    CleanExi HS_ITSN2.
    Genevestigatori Q9NZM3.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    2.60.40.150. 1 hit.
    InterProi IPR000008. C2_dom.
    IPR000219. DH-domain.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027029. Intersectin-2.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR11216:SF29. PTHR11216:SF29. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF00621. RhoGEF. 1 hit.
    PF00018. SH3_1. 2 hits.
    PF07653. SH3_2. 1 hit.
    PF14604. SH3_9. 2 hits.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00239. C2. 1 hit.
    SM00054. EFh. 2 hits.
    SM00027. EH. 2 hits.
    SM00233. PH. 1 hit.
    SM00325. RhoGEF. 1 hit.
    SM00326. SH3. 5 hits.
    [Graphical view ]
    SUPFAMi SSF48065. SSF48065. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF50044. SSF50044. 5 hits.
    PROSITEi PS50004. C2. 1 hit.
    PS50010. DH_2. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    PS50031. EH. 2 hits.
    PS50003. PH_DOMAIN. 1 hit.
    PS50002. SH3. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Intersectin 2, a new multimodular protein involved in clathrin-mediated endocytosis."
      Pucharcos C., Estivill X., de la Luna S.
      FEBS Lett. 478:43-51(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3).
      Tissue: Fetal brain and Fetal liver.
    2. "Intersectin 2 (SH3D1B), human homolog of mouse Ese2 protein."
      Seifert M., Engel M., Welter C.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
      Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
      DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 259-721 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1262-1697, VARIANT ILE-291.
      Tissue: Embryo.
    6. "SH3P18-like wasp associated protein (SWAP): a multiple SH3 domain containing protein that interacts with WASP."
      Wilson L.A., Fields D., Cruz L., Friesen J., Siminovitch K.A.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 822-1285 (ISOFORM 1).
    7. "Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins."
      Sparks A.B., Hoffman N.G., McConnell S.J., Fowlkes D.M., Kay B.K.
      Nat. Biotechnol. 14:741-744(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 945-1192 (ISOFORM 4).
      Tissue: Bone marrow.
    8. "The human intersectin genes and their spliced variants are differentially expressed."
      Pucharcos C., Casas C., Nadal M., Estivill X., de la Luna S.
      Biochim. Biophys. Acta 1521:1-11(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
      Tissue: Brain and Fetal liver.
    9. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
      Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C.
      Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-968, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    10. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-968, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-889, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    13. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
      Voss M., Lettau M., Janssen O.
      BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FASLG.
    14. "Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins."
      Kleino I., Ortiz R.M., Yritys M., Huovila A.P., Saksela K.
      J. Cell. Biochem. 108:877-885(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ADAM15.
    15. "Endocytic accessory proteins are functionally distinguished by their differential effects on the maturation of clathrin-coated pits."
      Mettlen M., Stoeber M., Loerke D., Antonescu C.N., Danuser G., Schmid S.L.
      Mol. Biol. Cell 20:3251-3260(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    16. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-210; SER-889 AND TYR-968, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230; THR-573; SER-889 AND TYR-968, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    18. "FCHo proteins are nucleators of clathrin-mediated endocytosis."
      Henne W.M., Boucrot E., Meinecke M., Evergren E., Vallis Y., Mittal R., McMahon H.T.
      Science 328:1281-1284(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FCHO2.
    19. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    20. "The clathrin adaptor Dab2 recruits EH domain scaffold proteins to regulate integrin beta1 endocytosis."
      Teckchandani A., Mulkearns E.E., Randolph T.W., Toida N., Cooper J.A.
      Mol. Biol. Cell 23:2905-2916(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    21. "Solution structure of SH3 domains of human intersectin 2 (KIAA1256)."
      RIKEN structural genomics initiative (RSGI)
      Submitted (AUG-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 762-1187.

    Entry informationi

    Entry nameiITSN2_HUMAN
    AccessioniPrimary (citable) accession number: Q9NZM3
    Secondary accession number(s): O95062
    , Q15812, Q9HAK4, Q9NXE6, Q9NYG0, Q9NZM2, Q9ULG4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2002
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 162 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Overexpression results in the inhibition of the transferrin uptake and the blockage of the clathrin-mediated endocytosis.

    Caution

    It is uncertain whether Met-1 or Met-2 is the initiator.Curated
    Studies in clathrin-mediated endocytosis of ITGB1 and TFR used a siRNA mixture of ISTN1 and ISTN2 suggesting a partially overlapping role of the EH domain-containing proteins.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3