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Protein

Zinc finger protein 224

Gene

ZNF224

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 198C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri204 – 226C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 254C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri260 – 282C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri288 – 310C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri316 – 338C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri344 – 366C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri372 – 394C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri400 – 422C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri428 – 450C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri456 – 478C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 506C2H2-type 12PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri512 – 534C2H2-type 13PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri540 – 562C2H2-type 14; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri568 – 590C2H2-type 15PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri596 – 618C2H2-type 16PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri652 – 674C2H2-type 17PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri680 – 702C2H2-type 18PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000178386-MONOMER.
ReactomeiR-HSA-212436. Generic Transcription Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 224
Alternative name(s):
Bone marrow zinc finger 2
Short name:
BMZF-2
Zinc finger protein 233
Zinc finger protein 255
Zinc finger protein 27
Zinc finger protein KOX22
Gene namesi
Name:ZNF224
Synonyms:BMZF2, KOX22, ZNF233, ZNF255, ZNF27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:13017. ZNF224.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: HPA
  • nucleus Source: UniProtKB
  • transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi7767.
OpenTargetsiENSG00000267680.
PharmGKBiPA37596.

Polymorphism and mutation databases

BioMutaiZNF224.
DMDMi313104253.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000474651 – 707Zinc finger protein 224Add BLAST707

Proteomic databases

MaxQBiQ9NZL3.
PaxDbiQ9NZL3.
PeptideAtlasiQ9NZL3.
PRIDEiQ9NZL3.

PTM databases

iPTMnetiQ9NZL3.
PhosphoSitePlusiQ9NZL3.

Expressioni

Tissue specificityi

Ubiquitous. Mainly expressed in fetal tissues.1 Publication

Gene expression databases

BgeeiENSG00000267680.
CleanExiHS_ZNF224.
HS_ZNF233.
ExpressionAtlasiQ9NZL3. baseline and differential.
GenevisibleiQ9NZL3. HS.

Organism-specific databases

HPAiHPA054045.

Interactioni

Subunit structurei

Interacts with WT1. Interacts with DEPDC1A.2 Publications

Protein-protein interaction databases

BioGridi113550. 11 interactors.
IntActiQ9NZL3. 1 interactor.
STRINGi9606.ENSP00000337368.

Structurei

Secondary structure

1707
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi174 – 177Combined sources4
Turni179 – 181Combined sources3
Beta strandi184 – 187Combined sources4
Helixi188 – 195Combined sources8
Turni196 – 198Combined sources3
Beta strandi207 – 209Combined sources3
Helixi216 – 223Combined sources8
Turni224 – 226Combined sources3
Beta strandi227 – 229Combined sources3
Beta strandi235 – 237Combined sources3
Beta strandi241 – 243Combined sources3
Helixi246 – 254Combined sources9
Beta strandi259 – 261Combined sources3
Turni263 – 265Combined sources3
Beta strandi268 – 271Combined sources4
Beta strandi283 – 285Combined sources3
Beta strandi291 – 293Combined sources3
Helixi300 – 309Combined sources10
Beta strandi313 – 317Combined sources5
Turni319 – 321Combined sources3
Beta strandi324 – 327Combined sources4
Helixi328 – 338Combined sources11
Beta strandi343 – 345Combined sources3
Beta strandi347 – 349Combined sources3
Beta strandi352 – 354Combined sources3
Helixi356 – 362Combined sources7
Helixi363 – 366Combined sources4
Beta strandi371 – 373Combined sources3
Beta strandi375 – 377Combined sources3
Beta strandi380 – 383Combined sources4
Helixi384 – 390Combined sources7
Helixi391 – 394Combined sources4
Beta strandi399 – 401Combined sources3
Beta strandi403 – 405Combined sources3
Beta strandi408 – 411Combined sources4
Helixi412 – 421Combined sources10
Beta strandi427 – 429Combined sources3
Beta strandi431 – 433Combined sources3
Beta strandi436 – 439Combined sources4
Helixi440 – 451Combined sources12
Turni459 – 462Combined sources4
Helixi468 – 477Combined sources10
Beta strandi480 – 482Combined sources3
Beta strandi487 – 489Combined sources3
Beta strandi493 – 495Combined sources3
Helixi496 – 502Combined sources7
Helixi503 – 506Combined sources4
Beta strandi511 – 513Combined sources3
Turni515 – 517Combined sources3
Beta strandi520 – 522Combined sources3
Helixi524 – 531Combined sources8
Beta strandi567 – 569Combined sources3
Turni571 – 573Combined sources3
Beta strandi576 – 579Combined sources4
Helixi580 – 586Combined sources7
Helixi587 – 590Combined sources4
Beta strandi655 – 657Combined sources3
Helixi664 – 670Combined sources7
Helixi671 – 675Combined sources5
Beta strandi683 – 685Combined sources3
Helixi692 – 698Combined sources7
Helixi699 – 702Combined sources4
Beta strandi703 – 706Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ELYNMR-A227-259[»]
2ELZNMR-A647-679[»]
2EM0NMR-A675-707[»]
2EM6NMR-A199-231[»]
2EM7NMR-A339-371[»]
2EM8NMR-A423-455[»]
2EM9NMR-A367-399[»]
2EN1NMR-A563-595[»]
2EN8NMR-A171-203[»]
2ENANMR-A311-343[»]
2ENCNMR-A395-427[»]
2EOQNMR-A283-315[»]
2EORNMR-A255-287[»]
2EQ4NMR-A451-483[»]
2YSPNMR-A507-539[»]
2YTHNMR-A479-511[»]
ProteinModelPortaliQ9NZL3.
SMRiQ9NZL3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NZL3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 78KRABPROSITE-ProRule annotationAdd BLAST71

Sequence similaritiesi

Contains 18 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri176 – 198C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri204 – 226C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri232 – 254C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri260 – 282C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri288 – 310C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri316 – 338C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri344 – 366C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri372 – 394C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri400 – 422C2H2-type 9PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri428 – 450C2H2-type 10PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri456 – 478C2H2-type 11PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri484 – 506C2H2-type 12PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri512 – 534C2H2-type 13PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri540 – 562C2H2-type 14; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri568 – 590C2H2-type 15PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri596 – 618C2H2-type 16PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri652 – 674C2H2-type 17PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri680 – 702C2H2-type 18PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129740.
HOVERGENiHBG018163.
InParanoidiQ9NZL3.
KOiK09228.
OMAiTWKCREC.
OrthoDBiEOG091G02KC.
PhylomeDBiQ9NZL3.
TreeFamiTF341885.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 20 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 18 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 18 hits.
PS50157. ZINC_FINGER_C2H2_2. 18 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NZL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTFKEAMTF KDVAVVFTEE ELGLLDLAQR KLYRDVMLEN FRNLLSVGHQ
60 70 80 90 100
AFHRDTFHFL REEKIWMMKT AIQREGNSGD KIQTEMETVS EAGTHQEWSF
110 120 130 140 150
QQIWEKIASD LTRSQDLMIN SSQFSKEGDF PCQTEAGLSV IHTRQKSSQG
160 170 180 190 200
NGYKPSFSDV SHFDFHQQLH SGEKSHTCDE CGKNFCYISA LRIHQRVHMG
210 220 230 240 250
EKCYKCDVCG KEFSQSSHLQ THQRVHTGEK PFKCVECGKG FSRRSALNVH
260 270 280 290 300
HKLHTGEKPY NCEECGKAFI HDSQLQEHQR IHTGEKPFKC DICGKSFCGR
310 320 330 340 350
SRLNRHSMVH TAEKPFRCDT CDKSFRQRSA LNSHRMIHTG EKPYKCEECG
360 370 380 390 400
KGFICRRDLY THHMVHTGEK PYNCKECGKS FRWASCLLKH QRVHSGEKPF
410 420 430 440 450
KCEECGKGFY TNSQCYSHQR SHSGEKPYKC VECGKGYKRR LDLDFHQRVH
460 470 480 490 500
TGEKLYNCKE CGKSFSRAPC LLKHERLHSG EKPFQCEECG KRFTQNSHLH
510 520 530 540 550
SHQRVHTGEK PYKCEKCGKG YNSKFNLDMH QKVHTGERPY NCKECGKSFG
560 570 580 590 600
WASCLLKHQR LHSGEKPFKC EECGKRFTQN SQLHSHQRVH TGEKPYKCDE
610 620 630 640 650
CGKGFSWSST RLTHQRRHSR ETPLKCEQHG KNIVQNSFSK VQEKVHSVEK
660 670 680 690 700
PYKCEDCGKG YNRRLNLDMH QRVHMGEKTW KCRECDMCFS QASSLRLHQN

VHVGEKP
Length:707
Mass (Da):82,280
Last modified:November 30, 2010 - v3
Checksum:iE9C1346D52BBF38F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti137 – 151GLSVI…SSQGN → RTICNSHKTEIFPRAI in AAD32448 (PubMed:15489334).CuratedAdd BLAST15
Sequence conflicti286K → T in CAA36579 (PubMed:12239212).Curated1
Sequence conflicti400 – 401FK → IQ in AAD32448 (PubMed:15489334).Curated2
Sequence conflicti412N → I in AAD32448 (PubMed:15489334).Curated1
Sequence conflicti521Y → H in AAD32448 (PubMed:15489334).Curated1
Sequence conflicti562H → R in AAF04106 (PubMed:12743021).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047061118M → V.4 PublicationsCorresponds to variant rs2068061dbSNPEnsembl.1
Natural variantiVAR_024204162H → L.3 PublicationsCorresponds to variant rs4239529dbSNPEnsembl.1
Natural variantiVAR_047062438K → N.Corresponds to variant rs3208201dbSNPEnsembl.1
Natural variantiVAR_061941447Q → H.Corresponds to variant rs58935748dbSNPEnsembl.1
Natural variantiVAR_047063506H → D.Corresponds to variant rs3746323dbSNPEnsembl.1
Natural variantiVAR_021891640K → E.2 PublicationsCorresponds to variant rs3746319dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187990 mRNA. Translation: AAF04106.2.
AC084219 Genomic DNA. No translation available.
AC114266 Genomic DNA. No translation available.
BC051902 mRNA. Translation: AAH51902.1.
AF067164 mRNA. Translation: AAD32448.1.
AY148489 mRNA. Translation: AAN61121.1.
X52353 mRNA. Translation: CAA36579.1.
AC021092 Genomic DNA. Translation: AAF24968.1.
CCDSiCCDS33046.1.
PIRiI37962.
RefSeqiNP_001308574.1. NM_001321645.1.
NP_037530.2. NM_013398.3.
XP_016882750.1. XM_017027261.1.
UniGeneiHs.585869.
Hs.709751.
Hs.744152.

Genome annotation databases

EnsembliENST00000336976; ENSP00000337368; ENSG00000267680.
GeneIDi7767.
KEGGihsa:7767.
UCSCiuc002oyh.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187990 mRNA. Translation: AAF04106.2.
AC084219 Genomic DNA. No translation available.
AC114266 Genomic DNA. No translation available.
BC051902 mRNA. Translation: AAH51902.1.
AF067164 mRNA. Translation: AAD32448.1.
AY148489 mRNA. Translation: AAN61121.1.
X52353 mRNA. Translation: CAA36579.1.
AC021092 Genomic DNA. Translation: AAF24968.1.
CCDSiCCDS33046.1.
PIRiI37962.
RefSeqiNP_001308574.1. NM_001321645.1.
NP_037530.2. NM_013398.3.
XP_016882750.1. XM_017027261.1.
UniGeneiHs.585869.
Hs.709751.
Hs.744152.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ELYNMR-A227-259[»]
2ELZNMR-A647-679[»]
2EM0NMR-A675-707[»]
2EM6NMR-A199-231[»]
2EM7NMR-A339-371[»]
2EM8NMR-A423-455[»]
2EM9NMR-A367-399[»]
2EN1NMR-A563-595[»]
2EN8NMR-A171-203[»]
2ENANMR-A311-343[»]
2ENCNMR-A395-427[»]
2EOQNMR-A283-315[»]
2EORNMR-A255-287[»]
2EQ4NMR-A451-483[»]
2YSPNMR-A507-539[»]
2YTHNMR-A479-511[»]
ProteinModelPortaliQ9NZL3.
SMRiQ9NZL3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113550. 11 interactors.
IntActiQ9NZL3. 1 interactor.
STRINGi9606.ENSP00000337368.

PTM databases

iPTMnetiQ9NZL3.
PhosphoSitePlusiQ9NZL3.

Polymorphism and mutation databases

BioMutaiZNF224.
DMDMi313104253.

Proteomic databases

MaxQBiQ9NZL3.
PaxDbiQ9NZL3.
PeptideAtlasiQ9NZL3.
PRIDEiQ9NZL3.

Protocols and materials databases

DNASUi7767.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336976; ENSP00000337368; ENSG00000267680.
GeneIDi7767.
KEGGihsa:7767.
UCSCiuc002oyh.3. human.

Organism-specific databases

CTDi7767.
DisGeNETi7767.
GeneCardsiZNF224.
HGNCiHGNC:13017. ZNF224.
HPAiHPA054045.
MIMi194555. gene.
neXtProtiNX_Q9NZL3.
OpenTargetsiENSG00000267680.
PharmGKBiPA37596.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129740.
HOVERGENiHBG018163.
InParanoidiQ9NZL3.
KOiK09228.
OMAiTWKCREC.
OrthoDBiEOG091G02KC.
PhylomeDBiQ9NZL3.
TreeFamiTF341885.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000178386-MONOMER.
ReactomeiR-HSA-212436. Generic Transcription Pathway.

Miscellaneous databases

EvolutionaryTraceiQ9NZL3.
GeneWikiiZNF224.
GenomeRNAii7767.
PROiQ9NZL3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000267680.
CleanExiHS_ZNF224.
HS_ZNF233.
ExpressionAtlasiQ9NZL3. baseline and differential.
GenevisibleiQ9NZL3. HS.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 20 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 18 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 18 hits.
PS50157. ZINC_FINGER_C2H2_2. 18 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN224_HUMAN
AccessioniPrimary (citable) accession number: Q9NZL3
Secondary accession number(s): A6NFW9
, P17033, Q86V10, Q8IZC8, Q9UID9, Q9Y2P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 30, 2010
Last modified: November 30, 2016
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.