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Protein

Zinc finger protein 224

Gene

ZNF224

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in transcriptional regulation as a transcriptional repressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri176 – 19823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri204 – 22623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri232 – 25423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri260 – 28223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri288 – 31023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri316 – 33823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri344 – 36623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri372 – 39423C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri400 – 42223C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri428 – 45023C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri456 – 47823C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri484 – 50623C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri512 – 53423C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri540 – 56223C2H2-type 14; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri568 – 59023C2H2-type 15PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri596 – 61823C2H2-type 16PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri652 – 67423C2H2-type 17PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri680 – 70223C2H2-type 18PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-212436. Generic Transcription Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 224
Alternative name(s):
Bone marrow zinc finger 2
Short name:
BMZF-2
Zinc finger protein 233
Zinc finger protein 255
Zinc finger protein 27
Zinc finger protein KOX22
Gene namesi
Name:ZNF224
Synonyms:BMZF2, KOX22, ZNF233, ZNF255, ZNF27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:13017. ZNF224.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: HPA
  • nucleus Source: UniProtKB
  • transcriptional repressor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37596.

Polymorphism and mutation databases

BioMutaiZNF224.
DMDMi313104253.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Zinc finger protein 224PRO_0000047465Add
BLAST

Proteomic databases

MaxQBiQ9NZL3.
PaxDbiQ9NZL3.
PeptideAtlasiQ9NZL3.
PRIDEiQ9NZL3.

PTM databases

iPTMnetiQ9NZL3.
PhosphoSiteiQ9NZL3.

Expressioni

Tissue specificityi

Ubiquitous. Mainly expressed in fetal tissues.1 Publication

Gene expression databases

BgeeiENSG00000267680.
CleanExiHS_ZNF224.
HS_ZNF233.
ExpressionAtlasiQ9NZL3. baseline and differential.
GenevisibleiQ9NZL3. HS.

Organism-specific databases

HPAiHPA054045.

Interactioni

Subunit structurei

Interacts with WT1. Interacts with DEPDC1A.2 Publications

Protein-protein interaction databases

BioGridi113550. 11 interactions.
STRINGi9606.ENSP00000337368.

Structurei

Secondary structure

1
707
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi174 – 1774Combined sources
Turni179 – 1813Combined sources
Beta strandi184 – 1874Combined sources
Helixi188 – 1958Combined sources
Turni196 – 1983Combined sources
Beta strandi207 – 2093Combined sources
Helixi216 – 2238Combined sources
Turni224 – 2263Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi235 – 2373Combined sources
Beta strandi241 – 2433Combined sources
Helixi246 – 2549Combined sources
Beta strandi259 – 2613Combined sources
Turni263 – 2653Combined sources
Beta strandi268 – 2714Combined sources
Beta strandi283 – 2853Combined sources
Beta strandi291 – 2933Combined sources
Helixi300 – 30910Combined sources
Beta strandi313 – 3175Combined sources
Turni319 – 3213Combined sources
Beta strandi324 – 3274Combined sources
Helixi328 – 33811Combined sources
Beta strandi343 – 3453Combined sources
Beta strandi347 – 3493Combined sources
Beta strandi352 – 3543Combined sources
Helixi356 – 3627Combined sources
Helixi363 – 3664Combined sources
Beta strandi371 – 3733Combined sources
Beta strandi375 – 3773Combined sources
Beta strandi380 – 3834Combined sources
Helixi384 – 3907Combined sources
Helixi391 – 3944Combined sources
Beta strandi399 – 4013Combined sources
Beta strandi403 – 4053Combined sources
Beta strandi408 – 4114Combined sources
Helixi412 – 42110Combined sources
Beta strandi427 – 4293Combined sources
Beta strandi431 – 4333Combined sources
Beta strandi436 – 4394Combined sources
Helixi440 – 45112Combined sources
Turni459 – 4624Combined sources
Helixi468 – 47710Combined sources
Beta strandi480 – 4823Combined sources
Beta strandi487 – 4893Combined sources
Beta strandi493 – 4953Combined sources
Helixi496 – 5027Combined sources
Helixi503 – 5064Combined sources
Beta strandi511 – 5133Combined sources
Turni515 – 5173Combined sources
Beta strandi520 – 5223Combined sources
Helixi524 – 5318Combined sources
Beta strandi567 – 5693Combined sources
Turni571 – 5733Combined sources
Beta strandi576 – 5794Combined sources
Helixi580 – 5867Combined sources
Helixi587 – 5904Combined sources
Beta strandi655 – 6573Combined sources
Helixi664 – 6707Combined sources
Helixi671 – 6755Combined sources
Beta strandi683 – 6853Combined sources
Helixi692 – 6987Combined sources
Helixi699 – 7024Combined sources
Beta strandi703 – 7064Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ELYNMR-A227-259[»]
2ELZNMR-A647-679[»]
2EM0NMR-A675-707[»]
2EM6NMR-A199-231[»]
2EM7NMR-A339-371[»]
2EM8NMR-A423-455[»]
2EM9NMR-A367-399[»]
2EN1NMR-A563-595[»]
2EN8NMR-A171-203[»]
2ENANMR-A311-343[»]
2ENCNMR-A395-427[»]
2EOQNMR-A283-315[»]
2EORNMR-A255-287[»]
2EQ4NMR-A451-483[»]
2YSPNMR-A507-539[»]
2YTHNMR-A479-511[»]
ProteinModelPortaliQ9NZL3.
SMRiQ9NZL3. Positions 156-707.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NZL3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 7871KRABPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 18 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri176 – 19823C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri204 – 22623C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri232 – 25423C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri260 – 28223C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri288 – 31023C2H2-type 5PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri316 – 33823C2H2-type 6PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri344 – 36623C2H2-type 7PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri372 – 39423C2H2-type 8PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri400 – 42223C2H2-type 9PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri428 – 45023C2H2-type 10PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri456 – 47823C2H2-type 11PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri484 – 50623C2H2-type 12PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri512 – 53423C2H2-type 13PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri540 – 56223C2H2-type 14; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri568 – 59023C2H2-type 15PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri596 – 61823C2H2-type 16PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri652 – 67423C2H2-type 17PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri680 – 70223C2H2-type 18PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129740.
HOVERGENiHBG018163.
InParanoidiQ9NZL3.
KOiK09228.
OMAiTWKCREC.
OrthoDBiEOG091G02KC.
PhylomeDBiQ9NZL3.
TreeFamiTF341885.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 19 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 18 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 18 hits.
PS50157. ZINC_FINGER_C2H2_2. 18 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NZL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTFKEAMTF KDVAVVFTEE ELGLLDLAQR KLYRDVMLEN FRNLLSVGHQ
60 70 80 90 100
AFHRDTFHFL REEKIWMMKT AIQREGNSGD KIQTEMETVS EAGTHQEWSF
110 120 130 140 150
QQIWEKIASD LTRSQDLMIN SSQFSKEGDF PCQTEAGLSV IHTRQKSSQG
160 170 180 190 200
NGYKPSFSDV SHFDFHQQLH SGEKSHTCDE CGKNFCYISA LRIHQRVHMG
210 220 230 240 250
EKCYKCDVCG KEFSQSSHLQ THQRVHTGEK PFKCVECGKG FSRRSALNVH
260 270 280 290 300
HKLHTGEKPY NCEECGKAFI HDSQLQEHQR IHTGEKPFKC DICGKSFCGR
310 320 330 340 350
SRLNRHSMVH TAEKPFRCDT CDKSFRQRSA LNSHRMIHTG EKPYKCEECG
360 370 380 390 400
KGFICRRDLY THHMVHTGEK PYNCKECGKS FRWASCLLKH QRVHSGEKPF
410 420 430 440 450
KCEECGKGFY TNSQCYSHQR SHSGEKPYKC VECGKGYKRR LDLDFHQRVH
460 470 480 490 500
TGEKLYNCKE CGKSFSRAPC LLKHERLHSG EKPFQCEECG KRFTQNSHLH
510 520 530 540 550
SHQRVHTGEK PYKCEKCGKG YNSKFNLDMH QKVHTGERPY NCKECGKSFG
560 570 580 590 600
WASCLLKHQR LHSGEKPFKC EECGKRFTQN SQLHSHQRVH TGEKPYKCDE
610 620 630 640 650
CGKGFSWSST RLTHQRRHSR ETPLKCEQHG KNIVQNSFSK VQEKVHSVEK
660 670 680 690 700
PYKCEDCGKG YNRRLNLDMH QRVHMGEKTW KCRECDMCFS QASSLRLHQN

VHVGEKP
Length:707
Mass (Da):82,280
Last modified:November 30, 2010 - v3
Checksum:iE9C1346D52BBF38F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti137 – 15115GLSVI…SSQGN → RTICNSHKTEIFPRAI in AAD32448 (PubMed:15489334).CuratedAdd
BLAST
Sequence conflicti286 – 2861K → T in CAA36579 (PubMed:12239212).Curated
Sequence conflicti400 – 4012FK → IQ in AAD32448 (PubMed:15489334).Curated
Sequence conflicti412 – 4121N → I in AAD32448 (PubMed:15489334).Curated
Sequence conflicti521 – 5211Y → H in AAD32448 (PubMed:15489334).Curated
Sequence conflicti562 – 5621H → R in AAF04106 (PubMed:12743021).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti118 – 1181M → V.4 Publications
Corresponds to variant rs2068061 [ dbSNP | Ensembl ].
VAR_047061
Natural varianti162 – 1621H → L.3 Publications
Corresponds to variant rs4239529 [ dbSNP | Ensembl ].
VAR_024204
Natural varianti438 – 4381K → N.
Corresponds to variant rs3208201 [ dbSNP | Ensembl ].
VAR_047062
Natural varianti447 – 4471Q → H.
Corresponds to variant rs58935748 [ dbSNP | Ensembl ].
VAR_061941
Natural varianti506 – 5061H → D.
Corresponds to variant rs3746323 [ dbSNP | Ensembl ].
VAR_047063
Natural varianti640 – 6401K → E.2 Publications
Corresponds to variant rs3746319 [ dbSNP | Ensembl ].
VAR_021891

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187990 mRNA. Translation: AAF04106.2.
AC084219 Genomic DNA. No translation available.
AC114266 Genomic DNA. No translation available.
BC051902 mRNA. Translation: AAH51902.1.
AF067164 mRNA. Translation: AAD32448.1.
AY148489 mRNA. Translation: AAN61121.1.
X52353 mRNA. Translation: CAA36579.1.
AC021092 Genomic DNA. Translation: AAF24968.1.
CCDSiCCDS33046.1.
PIRiI37962.
RefSeqiNP_001308574.1. NM_001321645.1.
NP_037530.2. NM_013398.3.
UniGeneiHs.585869.
Hs.709751.
Hs.744152.

Genome annotation databases

EnsembliENST00000336976; ENSP00000337368; ENSG00000267680.
GeneIDi7767.
KEGGihsa:7767.
UCSCiuc002oyh.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187990 mRNA. Translation: AAF04106.2.
AC084219 Genomic DNA. No translation available.
AC114266 Genomic DNA. No translation available.
BC051902 mRNA. Translation: AAH51902.1.
AF067164 mRNA. Translation: AAD32448.1.
AY148489 mRNA. Translation: AAN61121.1.
X52353 mRNA. Translation: CAA36579.1.
AC021092 Genomic DNA. Translation: AAF24968.1.
CCDSiCCDS33046.1.
PIRiI37962.
RefSeqiNP_001308574.1. NM_001321645.1.
NP_037530.2. NM_013398.3.
UniGeneiHs.585869.
Hs.709751.
Hs.744152.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ELYNMR-A227-259[»]
2ELZNMR-A647-679[»]
2EM0NMR-A675-707[»]
2EM6NMR-A199-231[»]
2EM7NMR-A339-371[»]
2EM8NMR-A423-455[»]
2EM9NMR-A367-399[»]
2EN1NMR-A563-595[»]
2EN8NMR-A171-203[»]
2ENANMR-A311-343[»]
2ENCNMR-A395-427[»]
2EOQNMR-A283-315[»]
2EORNMR-A255-287[»]
2EQ4NMR-A451-483[»]
2YSPNMR-A507-539[»]
2YTHNMR-A479-511[»]
ProteinModelPortaliQ9NZL3.
SMRiQ9NZL3. Positions 156-707.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113550. 11 interactions.
STRINGi9606.ENSP00000337368.

PTM databases

iPTMnetiQ9NZL3.
PhosphoSiteiQ9NZL3.

Polymorphism and mutation databases

BioMutaiZNF224.
DMDMi313104253.

Proteomic databases

MaxQBiQ9NZL3.
PaxDbiQ9NZL3.
PeptideAtlasiQ9NZL3.
PRIDEiQ9NZL3.

Protocols and materials databases

DNASUi7767.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336976; ENSP00000337368; ENSG00000267680.
GeneIDi7767.
KEGGihsa:7767.
UCSCiuc002oyh.3. human.

Organism-specific databases

CTDi7767.
GeneCardsiZNF224.
HGNCiHGNC:13017. ZNF224.
HPAiHPA054045.
MIMi194555. gene.
neXtProtiNX_Q9NZL3.
PharmGKBiPA37596.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129740.
HOVERGENiHBG018163.
InParanoidiQ9NZL3.
KOiK09228.
OMAiTWKCREC.
OrthoDBiEOG091G02KC.
PhylomeDBiQ9NZL3.
TreeFamiTF341885.

Enzyme and pathway databases

ReactomeiR-HSA-212436. Generic Transcription Pathway.

Miscellaneous databases

EvolutionaryTraceiQ9NZL3.
GeneWikiiZNF224.
GenomeRNAii7767.
PROiQ9NZL3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000267680.
CleanExiHS_ZNF224.
HS_ZNF233.
ExpressionAtlasiQ9NZL3. baseline and differential.
GenevisibleiQ9NZL3. HS.

Family and domain databases

CDDicd07765. KRAB_A-box. 1 hit.
Gene3Di3.30.160.60. 19 hits.
InterProiIPR001909. KRAB.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF01352. KRAB. 1 hit.
PF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 2 hits.
[Graphical view]
SMARTiSM00349. KRAB. 1 hit.
SM00355. ZnF_C2H2. 18 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 18 hits.
PS50157. ZINC_FINGER_C2H2_2. 18 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN224_HUMAN
AccessioniPrimary (citable) accession number: Q9NZL3
Secondary accession number(s): A6NFW9
, P17033, Q86V10, Q8IZC8, Q9UID9, Q9Y2P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 30, 2010
Last modified: September 7, 2016
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.