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Q9NZH0

- GPC5B_HUMAN

UniProt

Q9NZH0 - GPC5B_HUMAN

Protein

G-protein coupled receptor family C group 5 member B

Gene

GPRC5B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (13 Apr 2004)
      Previous versions | rss
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    Functioni

    Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. G-protein coupled receptor binding Source: UniProt
    3. protein kinase activator activity Source: MGI
    4. protein kinase binding Source: UniProt

    GO - Biological processi

    1. locomotory behavior Source: Ensembl
    2. positive regulation of canonical Wnt signaling pathway Source: UniProt
    3. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProt
    4. positive regulation of inflammatory response Source: UniProt
    5. positive regulation of macrophage cytokine production Source: UniProt
    6. positive regulation of neuron differentiation Source: UniProt
    7. positive regulation of neuron projection development Source: Ensembl
    8. positive regulation of protein tyrosine kinase activity Source: UniProt

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G-protein coupled receptor family C group 5 member B
    Alternative name(s):
    A-69G12.1
    Retinoic acid-induced gene 2 protein
    Short name:
    RAIG-2
    Gene namesi
    Name:GPRC5B
    Synonyms:RAIG2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:13308. GPRC5B.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Localized in the plasma membrane and perinuclear vesicles.

    GO - Cellular componenti

    1. cell surface Source: UniProt
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. extracellular space Source: UniProt
    4. extracellular vesicular exosome Source: UniProtKB
    5. integral component of membrane Source: UniProtKB-KW
    6. intracellular membrane-bounded organelle Source: HPA
    7. nucleolus Source: HPA
    8. nucleus Source: HPA
    9. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28938.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 403375G-protein coupled receptor family C group 5 member BPRO_0000012965Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9NZH0.
    PRIDEiQ9NZH0.

    PTM databases

    PhosphoSiteiQ9NZH0.

    Expressioni

    Tissue specificityi

    Expression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. According to PubMed:10945465, highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal chord as well as in dorsal root ganglia (DRG). In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach.2 Publications

    Inductioni

    By all-trans retinoic acid (ATRA).1 Publication

    Gene expression databases

    ArrayExpressiQ9NZH0.
    BgeeiQ9NZH0.
    CleanExiHS_GPRC5B.
    GenevestigatoriQ9NZH0.

    Organism-specific databases

    HPAiHPA015247.

    Interactioni

    Protein-protein interaction databases

    BioGridi119688. 3 interactions.
    IntActiQ9NZH0. 1 interaction.
    STRINGi9606.ENSP00000300571.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NZH0.
    SMRiQ9NZH0. Positions 27-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini29 – 5628ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini78 – 9417CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini116 – 12611ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini148 – 16215CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini184 – 19916ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini221 – 23414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini256 – 27116ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini293 – 403111CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei57 – 7721Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei95 – 11521Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei127 – 14721Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei163 – 18321Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei200 – 22021Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei235 – 25521Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei272 – 29221Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG150878.
    HOVERGENiHBG051794.
    InParanoidiQ9NZH0.
    KOiK04619.
    OrthoDBiEOG7RRF79.
    TreeFamiTF321410.

    Family and domain databases

    InterProiIPR017978. GPCR_3_C.
    [Graphical view]
    PfamiPF00003. 7tm_3. 1 hit.
    [Graphical view]
    PROSITEiPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NZH0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFVASERKMR AHQVLTFLLL FVITSVASEN ASTSRGCGLD LLPQYVSLCD    50
    LDAIWGIVVE AVAGAGALIT LLLMLILLVR LPFIKEKEKK SPVGLHFLFL 100
    LGTLGLFGLT FAFIIQEDET ICSVRRFLWG VLFALCFSCL LSQAWRVRRL 150
    VRHGTGPAGW QLVGLALCLM LVQVIIAVEW LVLTVLRDTR PACAYEPMDF 200
    VMALIYDMVL LVVTLGLALF TLCGKFKRWK LNGAFLLITA FLSVLIWVAW 250
    MTMYLFGNVK LQQGDAWNDP TLAITLAASG WVFVIFHAIP EIHCTLLPAL 300
    QENTPNYFDT SQPRMRETAF EEDVQLPRAY MENKAFSMDE HNAALRTAGF 350
    PNGSLGKRPS GSLGKRPSAP FRSNVYQPTE MAVVLNGGTI PTAPPSHTGR 400
    HLW 403
    Length:403
    Mass (Da):44,795
    Last modified:April 13, 2004 - v2
    Checksum:i3902A16C4F69C26E
    GO
    Isoform 2 (identifier: Q9NZH0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         390-403: IPTAPPSHTGRHLW → EMVTHPRSLESFGAF

    Show »
    Length:404
    Mass (Da):44,933
    Checksum:i4DE2B3914E6C396A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti46 – 461V → A in BAC11414. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei390 – 40314IPTAP…GRHLW → EMVTHPRSLESFGAF in isoform 2. 1 PublicationVSP_047585Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF202640 mRNA. Translation: AAF67321.1.
    AJ276101 mRNA. Translation: CAC00632.1.
    AF181862 mRNA. Translation: AAF05331.1.
    FJ529380 mRNA. Translation: ACU30030.1.
    AK075119 mRNA. Translation: BAC11414.1.
    AC004131 Genomic DNA. Translation: AAC27544.1.
    AC134300 Genomic DNA. No translation available.
    BC034467 mRNA. Translation: AAH34467.1.
    CCDSiCCDS10581.1. [Q9NZH0-1]
    RefSeqiNP_057319.1. NM_016235.1. [Q9NZH0-1]
    XP_006721114.1. XM_006721051.1. [Q9NZH0-1]
    XP_006721115.1. XM_006721052.1. [Q9NZH0-1]
    UniGeneiHs.148685.

    Genome annotation databases

    EnsembliENST00000300571; ENSP00000300571; ENSG00000167191. [Q9NZH0-1]
    ENST00000535671; ENSP00000442858; ENSG00000167191. [Q9NZH0-2]
    ENST00000569479; ENSP00000454727; ENSG00000167191. [Q9NZH0-1]
    ENST00000569847; ENSP00000457283; ENSG00000167191. [Q9NZH0-1]
    GeneIDi51704.
    KEGGihsa:51704.
    UCSCiuc002dgt.3. human. [Q9NZH0-1]

    Polymorphism databases

    DMDMi46396016.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF202640 mRNA. Translation: AAF67321.1 .
    AJ276101 mRNA. Translation: CAC00632.1 .
    AF181862 mRNA. Translation: AAF05331.1 .
    FJ529380 mRNA. Translation: ACU30030.1 .
    AK075119 mRNA. Translation: BAC11414.1 .
    AC004131 Genomic DNA. Translation: AAC27544.1 .
    AC134300 Genomic DNA. No translation available.
    BC034467 mRNA. Translation: AAH34467.1 .
    CCDSi CCDS10581.1. [Q9NZH0-1 ]
    RefSeqi NP_057319.1. NM_016235.1. [Q9NZH0-1 ]
    XP_006721114.1. XM_006721051.1. [Q9NZH0-1 ]
    XP_006721115.1. XM_006721052.1. [Q9NZH0-1 ]
    UniGenei Hs.148685.

    3D structure databases

    ProteinModelPortali Q9NZH0.
    SMRi Q9NZH0. Positions 27-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119688. 3 interactions.
    IntActi Q9NZH0. 1 interaction.
    STRINGi 9606.ENSP00000300571.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q9NZH0.

    Polymorphism databases

    DMDMi 46396016.

    Proteomic databases

    MaxQBi Q9NZH0.
    PRIDEi Q9NZH0.

    Protocols and materials databases

    DNASUi 51704.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000300571 ; ENSP00000300571 ; ENSG00000167191 . [Q9NZH0-1 ]
    ENST00000535671 ; ENSP00000442858 ; ENSG00000167191 . [Q9NZH0-2 ]
    ENST00000569479 ; ENSP00000454727 ; ENSG00000167191 . [Q9NZH0-1 ]
    ENST00000569847 ; ENSP00000457283 ; ENSG00000167191 . [Q9NZH0-1 ]
    GeneIDi 51704.
    KEGGi hsa:51704.
    UCSCi uc002dgt.3. human. [Q9NZH0-1 ]

    Organism-specific databases

    CTDi 51704.
    GeneCardsi GC16M019868.
    HGNCi HGNC:13308. GPRC5B.
    HPAi HPA015247.
    MIMi 605948. gene.
    neXtProti NX_Q9NZH0.
    PharmGKBi PA28938.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG150878.
    HOVERGENi HBG051794.
    InParanoidi Q9NZH0.
    KOi K04619.
    OrthoDBi EOG7RRF79.
    TreeFami TF321410.

    Miscellaneous databases

    GeneWikii GPRC5B.
    GenomeRNAii 51704.
    NextBioi 35489311.
    PROi Q9NZH0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NZH0.
    Bgeei Q9NZH0.
    CleanExi HS_GPRC5B.
    Genevestigatori Q9NZH0.

    Family and domain databases

    InterProi IPR017978. GPCR_3_C.
    [Graphical view ]
    Pfami PF00003. 7tm_3. 1 hit.
    [Graphical view ]
    PROSITEi PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain."
      Braeuner-Osborne H., Krogsgaard-Larsen P.
      Genomics 65:121-128(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)."
      Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N.
      Genomics 67:8-18(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
    3. "Cloning of a novel G protein-coupled receptor localized on human chromosome 16p12."
      Wei H., Osborne B., Spruyt M., Murphy D.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    4. "C-terminal splice variants of Gprc5b, an orphan G protein-coupled receptor that binds Frizzled Wnt receptors, are expressed in maturing neurons and influence neurite outgrowth."
      Cool B.H., Chan G.C.K., Lee L., Oshima J., Martin G.M., Hu Q.
      Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Brain.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Placenta.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The sequence and analysis of duplication-rich human chromosome 16."
      Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
      , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
      Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.

    Entry informationi

    Entry nameiGPC5B_HUMAN
    AccessioniPrimary (citable) accession number: Q9NZH0
    Secondary accession number(s): D2DFB0, O75205, Q8NBZ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: April 13, 2004
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-9 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    3. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3