Q9NZH0 (GPC5B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor family C group 5 member B

Alternative name(s):
A-69G12.1
Retinoic acid-induced gene 2 protein
Short name=RAIG-2

Gene names

Name:	GPRC5B
Synonyms:	RAIG2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Localized in the plasma membrane and perinuclear vesicles. Ref.2
Tissue specificity	Expression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. According to Ref.2, highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal chord as well as in dorsal root ganglia (DRG). In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach. Ref.1 Ref.2
Induction	By all-trans retinoic acid (ATRA). Ref.2
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.
Caution	It is uncertain whether Met-1 or Met-9 is the initiator.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Golgi apparatus Inferred from direct assay. Source: HPA cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular vesicular exosome Inferred from direct assay PubMed 21557262. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Inferred from direct assay. Source: HPA plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 403	375	G-protein coupled receptor family C group 5 member B		PRO_0000012965
Regions
	Topological domain	29 – 56	28	Extracellular Potential
	Transmembrane	57 – 77	21	Helical; Name=1; Potential
	Topological domain	78 – 94	17	Cytoplasmic Potential
	Transmembrane	95 – 115	21	Helical; Name=2; Potential
	Topological domain	116 – 126	11	Extracellular Potential
	Transmembrane	127 – 147	21	Helical; Name=3; Potential
	Topological domain	148 – 162	15	Cytoplasmic Potential
	Transmembrane	163 – 183	21	Helical; Name=4; Potential
	Topological domain	184 – 199	16	Extracellular Potential
	Transmembrane	200 – 220	21	Helical; Name=5; Potential
	Topological domain	221 – 234	14	Cytoplasmic Potential
	Transmembrane	235 – 255	21	Helical; Name=6; Potential
	Topological domain	256 – 271	16	Extracellular Potential
	Transmembrane	272 – 292	21	Helical; Name=7; Potential
	Topological domain	293 – 403	111	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	46	1	V → A in BAC11414. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9NZH0 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 3902A16C4F69C26E	FASTA	403	44,795
	10 20 30 40 50 60 MFVASERKMR AHQVLTFLLL FVITSVASEN ASTSRGCGLD LLPQYVSLCD LDAIWGIVVE 70 80 90 100 110 120 AVAGAGALIT LLLMLILLVR LPFIKEKEKK SPVGLHFLFL LGTLGLFGLT FAFIIQEDET 130 140 150 160 170 180 ICSVRRFLWG VLFALCFSCL LSQAWRVRRL VRHGTGPAGW QLVGLALCLM LVQVIIAVEW 190 200 210 220 230 240 LVLTVLRDTR PACAYEPMDF VMALIYDMVL LVVTLGLALF TLCGKFKRWK LNGAFLLITA 250 260 270 280 290 300 FLSVLIWVAW MTMYLFGNVK LQQGDAWNDP TLAITLAASG WVFVIFHAIP EIHCTLLPAL 310 320 330 340 350 360 QENTPNYFDT SQPRMRETAF EEDVQLPRAY MENKAFSMDE HNAALRTAGF PNGSLGKRPS 370 380 390 400 GSLGKRPSAP FRSNVYQPTE MAVVLNGGTI PTAPPSHTGR HLW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain." Braeuner-Osborne H., Krogsgaard-Larsen P. Genomics 65:121-128(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)." Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N. Genomics 67:8-18(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[3]	"Cloning of a novel G protein-coupled receptor localized on human chromosome 16p12." Wei H., Osborne B., Spruyt M., Murphy D. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	"Homo sapiens chromosome 16 BAC clone CIT987SK-A-69G12." Adams M.D., Loftus B.J., Zhou L., Crosby M., Fuhrmann J., Mason T.M., Brandon R., Kim U.J., Kerlavage A.R., Venter J.C. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF202640 mRNA. Translation: AAF67321.1. AJ276101 mRNA. Translation: CAC00632.1. AF181862 mRNA. Translation: AAF05331.1. AK075119 mRNA. Translation: BAC11414.1. AC004131 Genomic DNA. Translation: AAC27544.1. BC034467 mRNA. Translation: AAH34467.1.
IPI	IPI01012002.
RefSeq	NP_057319.1. NM_016235.1.
UniGene	Hs.148685.
3D structure databases
ProteinModelPortal	Q9NZH0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NZH0. 1 interaction.
STRING	9606.ENSP00000300571.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9NZH0.
Polymorphism databases
DMDM	46396016.
Proteomic databases
PRIDE	Q9NZH0.
Protocols and materials databases
DNASU	51704.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000300571; ENSP00000300571; ENSG00000167191. ENST00000569479; ENSP00000454727; ENSG00000167191. ENST00000569847; ENSP00000457283; ENSG00000167191.
GeneID	51704.
KEGG	hsa:51704.
UCSC	uc002dgt.3. human.
Organism-specific databases
CTD	51704.
GeneCards	GC16M019868.
HGNC	HGNC:13308. GPRC5B.
HPA	HPA015247.
MIM	605948. gene.
neXtProt	NX_Q9NZH0.
PharmGKB	PA28938.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG150878.
HOVERGEN	HBG051794.
InParanoid	Q9NZH0.
KO	K04619.
OrthoDB	EOG4229KM.
PhylomeDB	Q9NZH0.
Gene expression databases
ArrayExpress	Q9NZH0.
Bgee	Q9NZH0.
CleanEx	HS_GPRC5B.
Genevestigator	Q9NZH0.
GermOnline	ENSG00000167191. Homo sapiens.
Family and domain databases
InterPro	IPR017978. GPCR_3_C. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. [Graphical view]
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. False negative. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51704.
NextBio	55740.
SOURCE	Search...

Entry information

Entry name

GPC5B_HUMAN

Accession

Primary (citable) accession number: Q9NZH0
Secondary accession number(s): O75205, Q8NBZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.