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Protein

G-protein coupled receptor family C group 5 member B

Gene

GPRC5B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. G-protein coupled receptor binding Source: UniProtKB
  3. protein kinase activator activity Source: MGI
  4. protein kinase binding Source: UniProtKB

GO - Biological processi

  1. glucose homeostasis Source: Ensembl
  2. locomotory behavior Source: Ensembl
  3. positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  4. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  5. positive regulation of inflammatory response Source: UniProtKB
  6. positive regulation of macrophage cytokine production Source: UniProtKB
  7. positive regulation of neuron differentiation Source: UniProtKB
  8. positive regulation of neuron projection development Source: Ensembl
  9. positive regulation of protein tyrosine kinase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor family C group 5 member B
Alternative name(s):
A-69G12.1
Retinoic acid-induced gene 2 protein
Short name:
RAIG-2
Gene namesi
Name:GPRC5B
Synonyms:RAIG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:13308. GPRC5B.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cytoplasmic vesicle membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Localized in the plasma membrane and perinuclear vesicles.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 5628ExtracellularSequence AnalysisAdd
BLAST
Transmembranei57 – 7721Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini78 – 9417CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei95 – 11521Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini116 – 12611ExtracellularSequence AnalysisAdd
BLAST
Transmembranei127 – 14721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini148 – 16215CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei163 – 18321Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini184 – 19916ExtracellularSequence AnalysisAdd
BLAST
Transmembranei200 – 22021Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini221 – 23414CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei235 – 25521Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini256 – 27116ExtracellularSequence AnalysisAdd
BLAST
Transmembranei272 – 29221Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini293 – 403111CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: UniProtKB
  2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  3. extracellular space Source: UniProtKB
  4. extracellular vesicular exosome Source: UniProtKB
  5. integral component of membrane Source: UniProtKB-KW
  6. intracellular membrane-bounded organelle Source: HPA
  7. membrane raft Source: Ensembl
  8. nucleolus Source: HPA
  9. nucleus Source: HPA
  10. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28938.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 403375G-protein coupled receptor family C group 5 member BPRO_0000012965Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9NZH0.
PRIDEiQ9NZH0.

PTM databases

PhosphoSiteiQ9NZH0.

Expressioni

Tissue specificityi

Expression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. According to PubMed:10945465, highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal chord as well as in dorsal root ganglia (DRG). In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach.2 Publications

Inductioni

By all-trans retinoic acid (ATRA).1 Publication

Gene expression databases

BgeeiQ9NZH0.
CleanExiHS_GPRC5B.
ExpressionAtlasiQ9NZH0. baseline and differential.
GenevestigatoriQ9NZH0.

Organism-specific databases

HPAiHPA015247.

Interactioni

Protein-protein interaction databases

BioGridi119688. 6 interactions.
IntActiQ9NZH0. 1 interaction.
STRINGi9606.ENSP00000300571.

Structurei

3D structure databases

ProteinModelPortaliQ9NZH0.
SMRiQ9NZH0. Positions 27-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG150878.
GeneTreeiENSGT00520000055551.
HOVERGENiHBG051794.
InParanoidiQ9NZH0.
KOiK04619.
OrthoDBiEOG7RRF79.
TreeFamiTF321410.

Family and domain databases

InterProiIPR017978. GPCR_3_C.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
[Graphical view]
PROSITEiPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZH0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFVASERKMR AHQVLTFLLL FVITSVASEN ASTSRGCGLD LLPQYVSLCD
60 70 80 90 100
LDAIWGIVVE AVAGAGALIT LLLMLILLVR LPFIKEKEKK SPVGLHFLFL
110 120 130 140 150
LGTLGLFGLT FAFIIQEDET ICSVRRFLWG VLFALCFSCL LSQAWRVRRL
160 170 180 190 200
VRHGTGPAGW QLVGLALCLM LVQVIIAVEW LVLTVLRDTR PACAYEPMDF
210 220 230 240 250
VMALIYDMVL LVVTLGLALF TLCGKFKRWK LNGAFLLITA FLSVLIWVAW
260 270 280 290 300
MTMYLFGNVK LQQGDAWNDP TLAITLAASG WVFVIFHAIP EIHCTLLPAL
310 320 330 340 350
QENTPNYFDT SQPRMRETAF EEDVQLPRAY MENKAFSMDE HNAALRTAGF
360 370 380 390 400
PNGSLGKRPS GSLGKRPSAP FRSNVYQPTE MAVVLNGGTI PTAPPSHTGR

HLW
Length:403
Mass (Da):44,795
Last modified:April 12, 2004 - v2
Checksum:i3902A16C4F69C26E
GO
Isoform 2 (identifier: Q9NZH0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-403: IPTAPPSHTGRHLW → EMVTHPRSLESFGAF

Show »
Length:404
Mass (Da):44,933
Checksum:i4DE2B3914E6C396A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461V → A in BAC11414 (PubMed:14702039).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei390 – 40314IPTAP…GRHLW → EMVTHPRSLESFGAF in isoform 2. 1 PublicationVSP_047585Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202640 mRNA. Translation: AAF67321.1.
AJ276101 mRNA. Translation: CAC00632.1.
AF181862 mRNA. Translation: AAF05331.1.
FJ529380 mRNA. Translation: ACU30030.1.
AK075119 mRNA. Translation: BAC11414.1.
AC004131 Genomic DNA. Translation: AAC27544.1.
AC134300 Genomic DNA. No translation available.
BC034467 mRNA. Translation: AAH34467.1.
CCDSiCCDS10581.1. [Q9NZH0-1]
RefSeqiNP_057319.1. NM_016235.2. [Q9NZH0-1]
XP_006721114.1. XM_006721051.1. [Q9NZH0-1]
XP_006721115.1. XM_006721052.1. [Q9NZH0-1]
UniGeneiHs.148685.

Genome annotation databases

EnsembliENST00000300571; ENSP00000300571; ENSG00000167191. [Q9NZH0-1]
ENST00000535671; ENSP00000442858; ENSG00000167191. [Q9NZH0-2]
ENST00000569479; ENSP00000454727; ENSG00000167191. [Q9NZH0-1]
ENST00000569847; ENSP00000457283; ENSG00000167191. [Q9NZH0-1]
GeneIDi51704.
KEGGihsa:51704.
UCSCiuc002dgt.3. human. [Q9NZH0-1]

Polymorphism databases

DMDMi46396016.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202640 mRNA. Translation: AAF67321.1.
AJ276101 mRNA. Translation: CAC00632.1.
AF181862 mRNA. Translation: AAF05331.1.
FJ529380 mRNA. Translation: ACU30030.1.
AK075119 mRNA. Translation: BAC11414.1.
AC004131 Genomic DNA. Translation: AAC27544.1.
AC134300 Genomic DNA. No translation available.
BC034467 mRNA. Translation: AAH34467.1.
CCDSiCCDS10581.1. [Q9NZH0-1]
RefSeqiNP_057319.1. NM_016235.2. [Q9NZH0-1]
XP_006721114.1. XM_006721051.1. [Q9NZH0-1]
XP_006721115.1. XM_006721052.1. [Q9NZH0-1]
UniGeneiHs.148685.

3D structure databases

ProteinModelPortaliQ9NZH0.
SMRiQ9NZH0. Positions 27-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119688. 6 interactions.
IntActiQ9NZH0. 1 interaction.
STRINGi9606.ENSP00000300571.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9NZH0.

Polymorphism databases

DMDMi46396016.

Proteomic databases

MaxQBiQ9NZH0.
PRIDEiQ9NZH0.

Protocols and materials databases

DNASUi51704.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300571; ENSP00000300571; ENSG00000167191. [Q9NZH0-1]
ENST00000535671; ENSP00000442858; ENSG00000167191. [Q9NZH0-2]
ENST00000569479; ENSP00000454727; ENSG00000167191. [Q9NZH0-1]
ENST00000569847; ENSP00000457283; ENSG00000167191. [Q9NZH0-1]
GeneIDi51704.
KEGGihsa:51704.
UCSCiuc002dgt.3. human. [Q9NZH0-1]

Organism-specific databases

CTDi51704.
GeneCardsiGC16M019868.
HGNCiHGNC:13308. GPRC5B.
HPAiHPA015247.
MIMi605948. gene.
neXtProtiNX_Q9NZH0.
PharmGKBiPA28938.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG150878.
GeneTreeiENSGT00520000055551.
HOVERGENiHBG051794.
InParanoidiQ9NZH0.
KOiK04619.
OrthoDBiEOG7RRF79.
TreeFamiTF321410.

Miscellaneous databases

ChiTaRSiGPRC5B. human.
GeneWikiiGPRC5B.
GenomeRNAii51704.
NextBioi35489311.
PROiQ9NZH0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NZH0.
CleanExiHS_GPRC5B.
ExpressionAtlasiQ9NZH0. baseline and differential.
GenevestigatoriQ9NZH0.

Family and domain databases

InterProiIPR017978. GPCR_3_C.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
[Graphical view]
PROSITEiPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain."
    Braeuner-Osborne H., Krogsgaard-Larsen P.
    Genomics 65:121-128(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
  2. "Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)."
    Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N.
    Genomics 67:8-18(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
  3. "Cloning of a novel G protein-coupled receptor localized on human chromosome 16p12."
    Wei H., Osborne B., Spruyt M., Murphy D.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. "C-terminal splice variants of Gprc5b, an orphan G protein-coupled receptor that binds Frizzled Wnt receptors, are expressed in maturing neurons and influence neurite outgrowth."
    Cool B.H., Chan G.C.K., Lee L., Oshima J., Martin G.M., Hu Q.
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.

Entry informationi

Entry nameiGPC5B_HUMAN
AccessioniPrimary (citable) accession number: Q9NZH0
Secondary accession number(s): D2DFB0, O75205, Q8NBZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2004
Last sequence update: April 12, 2004
Last modified: March 31, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.