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Q9NZH0 (GPC5B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor family C group 5 member B
Alternative name(s):
A-69G12.1
Retinoic acid-induced gene 2 protein
Short name=RAIG-2
Gene names
Name:GPRC5B
Synonyms:RAIG2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Unknown. This retinoic acid-inducible G-protein coupled receptor provide evidence for a possible interaction between retinoid and G-protein signaling pathways.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Localized in the plasma membrane and perinuclear vesicles. Ref.2

Tissue specificity

Expression is high in kidney, pancreas, and testis, medium in brain, heart, prostate, small intestine, and spleen, low in liver, placenta, skeletal muscle, colon, ovary, and thymus, and not detectable in lung and peripheral leukocyte. According to Ref.2, highly expressed in most brain areas examined, with the highest levels observed in corpus callosum, caudate nucleus, putamen, substantia nigra, thalamus, hippocampus, and spinal chord as well as in dorsal root ganglia (DRG). In the periphery, expression levels are relatively low, compared to the CNS, with the strongest expression detected in pancreas, testis, uterus, and stomach. Ref.1 Ref.2

Induction

By all-trans retinoic acid (ATRA). Ref.2

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasmic vesicle
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlocomotory behavior

Inferred from electronic annotation. Source: Ensembl

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from sequence or structural similarity. Source: UniProt

positive regulation of canonical Wnt signaling pathway

Inferred from sequence or structural similarity. Source: UniProt

positive regulation of inflammatory response

Inferred from sequence or structural similarity. Source: UniProt

positive regulation of macrophage cytokine production

Inferred from sequence or structural similarity. Source: UniProt

positive regulation of neuron differentiation

Inferred from sequence or structural similarity. Source: UniProt

positive regulation of neuron projection development

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein tyrosine kinase activity

Inferred from sequence or structural similarity. Source: UniProt

   Cellular_componentcell surface

Inferred from sequence or structural similarity. Source: UniProt

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular vesicular exosome

Inferred from direct assay PubMed 21557262. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

nucleolus

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

G-protein coupled receptor binding

Inferred from sequence or structural similarity. Source: UniProt

protein kinase activator activity

Inferred from direct assay PubMed 23169819. Source: MGI

protein kinase binding

Inferred from sequence or structural similarity. Source: UniProt

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NZH0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NZH0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     390-403: IPTAPPSHTGRHLW → EMVTHPRSLESFGAF

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 403375G-protein coupled receptor family C group 5 member B
PRO_0000012965

Regions

Topological domain29 – 5628Extracellular Potential
Transmembrane57 – 7721Helical; Name=1; Potential
Topological domain78 – 9417Cytoplasmic Potential
Transmembrane95 – 11521Helical; Name=2; Potential
Topological domain116 – 12611Extracellular Potential
Transmembrane127 – 14721Helical; Name=3; Potential
Topological domain148 – 16215Cytoplasmic Potential
Transmembrane163 – 18321Helical; Name=4; Potential
Topological domain184 – 19916Extracellular Potential
Transmembrane200 – 22021Helical; Name=5; Potential
Topological domain221 – 23414Cytoplasmic Potential
Transmembrane235 – 25521Helical; Name=6; Potential
Topological domain256 – 27116Extracellular Potential
Transmembrane272 – 29221Helical; Name=7; Potential
Topological domain293 – 403111Cytoplasmic Potential

Amino acid modifications

Glycosylation301N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence390 – 40314IPTAP…GRHLW → EMVTHPRSLESFGAF in isoform 2.
VSP_047585

Experimental info

Sequence conflict461V → A in BAC11414. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 3902A16C4F69C26E

FASTA40344,795
        10         20         30         40         50         60 
MFVASERKMR AHQVLTFLLL FVITSVASEN ASTSRGCGLD LLPQYVSLCD LDAIWGIVVE 

        70         80         90        100        110        120 
AVAGAGALIT LLLMLILLVR LPFIKEKEKK SPVGLHFLFL LGTLGLFGLT FAFIIQEDET 

       130        140        150        160        170        180 
ICSVRRFLWG VLFALCFSCL LSQAWRVRRL VRHGTGPAGW QLVGLALCLM LVQVIIAVEW 

       190        200        210        220        230        240 
LVLTVLRDTR PACAYEPMDF VMALIYDMVL LVVTLGLALF TLCGKFKRWK LNGAFLLITA 

       250        260        270        280        290        300 
FLSVLIWVAW MTMYLFGNVK LQQGDAWNDP TLAITLAASG WVFVIFHAIP EIHCTLLPAL 

       310        320        330        340        350        360 
QENTPNYFDT SQPRMRETAF EEDVQLPRAY MENKAFSMDE HNAALRTAGF PNGSLGKRPS 

       370        380        390        400 
GSLGKRPSAP FRSNVYQPTE MAVVLNGGTI PTAPPSHTGR HLW 

« Hide

Isoform 2 [UniParc].

Checksum: 4DE2B3914E6C396A
Show »

FASTA40444,933

References

« Hide 'large scale' references
[1]"Sequence and expression pattern of a novel human orphan G-protein-coupled receptor, GPRC5B, a family C receptor with a short amino-terminal domain."
Braeuner-Osborne H., Krogsgaard-Larsen P.
Genomics 65:121-128(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
[2]"Molecular cloning and characterization of two novel retinoic acid-inducible orphan G-protein-coupled receptors (GPRC5B and GPRC5C)."
Robbins M.J., Michalovich D., Hill J., Calver A.R., Medhurst A.D., Gloger I., Sims M.A., Middlemiss D.N., Pangalos M.N.
Genomics 67:8-18(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[3]"Cloning of a novel G protein-coupled receptor localized on human chromosome 16p12."
Wei H., Osborne B., Spruyt M., Murphy D.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[4]"C-terminal splice variants of Gprc5b, an orphan G protein-coupled receptor that binds Frizzled Wnt receptors, are expressed in maturing neurons and influence neurite outgrowth."
Cool B.H., Chan G.C.K., Lee L., Oshima J., Martin G.M., Hu Q.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[6]"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q."
Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D.
Genomics 60:295-308(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF202640 mRNA. Translation: AAF67321.1.
AJ276101 mRNA. Translation: CAC00632.1.
AF181862 mRNA. Translation: AAF05331.1.
FJ529380 mRNA. Translation: ACU30030.1.
AK075119 mRNA. Translation: BAC11414.1.
AC004131 Genomic DNA. Translation: AAC27544.1.
AC134300 Genomic DNA. No translation available.
BC034467 mRNA. Translation: AAH34467.1.
CCDSCCDS10581.1. [Q9NZH0-1]
RefSeqNP_057319.1. NM_016235.1. [Q9NZH0-1]
XP_006721114.1. XM_006721051.1. [Q9NZH0-1]
XP_006721115.1. XM_006721052.1. [Q9NZH0-1]
UniGeneHs.148685.

3D structure databases

ProteinModelPortalQ9NZH0.
SMRQ9NZH0. Positions 27-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119688. 2 interactions.
IntActQ9NZH0. 1 interaction.
STRING9606.ENSP00000300571.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9NZH0.

Polymorphism databases

DMDM46396016.

Proteomic databases

MaxQBQ9NZH0.
PRIDEQ9NZH0.

Protocols and materials databases

DNASU51704.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300571; ENSP00000300571; ENSG00000167191. [Q9NZH0-1]
ENST00000535671; ENSP00000442858; ENSG00000167191. [Q9NZH0-2]
ENST00000569479; ENSP00000454727; ENSG00000167191. [Q9NZH0-1]
ENST00000569847; ENSP00000457283; ENSG00000167191. [Q9NZH0-1]
GeneID51704.
KEGGhsa:51704.
UCSCuc002dgt.3. human. [Q9NZH0-1]

Organism-specific databases

CTD51704.
GeneCardsGC16M019868.
HGNCHGNC:13308. GPRC5B.
HPAHPA015247.
MIM605948. gene.
neXtProtNX_Q9NZH0.
PharmGKBPA28938.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG150878.
HOVERGENHBG051794.
InParanoidQ9NZH0.
KOK04619.
OrthoDBEOG7RRF79.
TreeFamTF321410.

Gene expression databases

ArrayExpressQ9NZH0.
BgeeQ9NZH0.
CleanExHS_GPRC5B.
GenevestigatorQ9NZH0.

Family and domain databases

InterProIPR017978. GPCR_3_C.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
[Graphical view]
PROSITEPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPRC5B.
GenomeRNAi51704.
NextBio35489311.
PROQ9NZH0.
SOURCESearch...

Entry information

Entry nameGPC5B_HUMAN
AccessionPrimary (citable) accession number: Q9NZH0
Secondary accession number(s): D2DFB0, O75205, Q8NBZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: July 9, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries