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Protein

ETS homologous factor

Gene

EHF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.5 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi207 – 289ETSPROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • epithelial cell differentiation Source: UniProtKB
  • epithelial cell proliferation Source: InterPro
  • multicellular organism development Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135373-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS homologous factor
Short name:
hEHF
Alternative name(s):
ETS domain-containing transcription factor
Epithelium-specific Ets transcription factor 3
Short name:
ESE-3
Gene namesi
Name:EHFImported
Synonyms:ESE3, ESE3BImported, ESEJImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:3246. EHF.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi26298.
OpenTargetsiENSG00000135373.
PharmGKBiPA27681.

Polymorphism and mutation databases

BioMutaiEHF.
DMDMi74762761.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002579691 – 300ETS homologous factorAdd BLAST300

Proteomic databases

MaxQBiQ9NZC4.
PaxDbiQ9NZC4.
PeptideAtlasiQ9NZC4.
PRIDEiQ9NZC4.

PTM databases

iPTMnetiQ9NZC4.
PhosphoSitePlusiQ9NZC4.

Expressioni

Tissue specificityi

Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, testis, ovary, small intestine or peripheral blood leukocytes.4 Publications

Inductioni

By IL1B/interleukin-1 beta and TNF in bronchial smooth muscle cells and fibroblasts. By IL10/interleukin-10 and IFNG/IFN-gamma in monocyte-derived dendritic cells.2 Publications

Gene expression databases

BgeeiENSG00000135373.
CleanExiHS_EHF.
ExpressionAtlasiQ9NZC4. baseline and differential.
GenevisibleiQ9NZC4. HS.

Organism-specific databases

HPAiHPA050923.
HPA060734.

Interactioni

Protein-protein interaction databases

BioGridi117675. 8 interactors.
IntActiQ9NZC4. 1 interactor.
MINTiMINT-8247463.
STRINGi9606.ENSP00000435835.

Structurei

3D structure databases

ProteinModelPortaliQ9NZC4.
SMRiQ9NZC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 115PNTPROSITE-ProRule annotationAdd BLAST87

Domaini

The PNT domain acts as a transcriptional activator.By similarity

Sequence similaritiesi

Belongs to the ETS family.Sequence analysis
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOVERGENiHBG074143.
InParanoidiQ9NZC4.
KOiK17102.
OMAiPFQEFDV.
OrthoDBiEOG091G0BE6.
PhylomeDBiQ9NZC4.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033071. EHF.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF171. PTHR11849:SF171. 2 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 13 Publications (identifier: Q9NZC4-1) [UniParc]FASTAAdd to basket
Also known as: ESE-3B1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MILEGGGVMN LNPGNNLLHQ PPAWTDSYST CNVSSGFFGG QWHEIHPQYW
60 70 80 90 100
TKYQVWEWLQ HLLDTNQLDA NCIPFQEFDI NGEHLCSMSL QEFTRAAGTA
110 120 130 140 150
GQLLYSNLQH LKWNGQCSSD LFQSTHNVIV KTEQTEPSIM NTWKDENYLY
160 170 180 190 200
DTNYGSTVDL LDSKTFCRAQ ISMTTTSHLP VAESPDMKKE QDPPAKCHTK
210 220 230 240 250
KHNPRGTHLW EFIRDILLNP DKNPGLIKWE DRSEGVFRFL KSEAVAQLWG
260 270 280 290 300
KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN
Length:300
Mass (Da):34,892
Last modified:October 1, 2000 - v1
Checksum:i5CABB38BAF852ED0
GO
Isoform 21 Publication (identifier: Q9NZC4-2) [UniParc]FASTAAdd to basket
Also known as: ESE-3A1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     159-181: Missing.

Show »
Length:277
Mass (Da):32,345
Checksum:i3BF620E8F1164811
GO
Isoform 3 (identifier: Q9NZC4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGLPERRGLVLLLSLAEILFKIM

Note: No experimental confirmation available.
Show »
Length:322
Mass (Da):37,356
Checksum:i62D08E04E9C0FFDE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40G → S in AAD30990 (PubMed:10644770).Curated1
Sequence conflicti40G → S in AAD30991 (PubMed:10644770).Curated1
Sequence conflicti163S → G in AAD30991 (PubMed:10644770).Curated1
Sequence conflicti169A → V in AAD30991 (PubMed:10644770).Curated1
Sequence conflicti196K → E in AAF06998 (PubMed:10527851).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04894196A → V.Corresponds to variant rs9804460dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0463731M → MGLPERRGLVLLLSLAEILF KIM in isoform 3. 1 Publication1
Alternative sequenceiVSP_052190159 – 181Missing in isoform 2. 1 PublicationAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170583 mRNA. Translation: AAF06998.1.
AF124438 mRNA. Translation: AAD30990.1.
AF124439 mRNA. Translation: AAD30991.1.
AF212848 mRNA. Translation: AAF61670.1.
AF203977 mRNA. Translation: AAG35644.1.
AY882601 mRNA. Translation: AAW80658.1.
AK310867 mRNA. No translation available.
AC087783 Genomic DNA. No translation available.
AL157952 Genomic DNA. Translation: CAC12700.1.
AL157952 Genomic DNA. Translation: CAC12701.1.
CH471064 Genomic DNA. Translation: EAW68162.1.
CH471064 Genomic DNA. Translation: EAW68163.1.
CH471064 Genomic DNA. Translation: EAW68164.1.
CH471064 Genomic DNA. Translation: EAW68165.1.
CH471064 Genomic DNA. Translation: EAW68166.1.
BC038995 mRNA. Translation: AAH38995.1.
CCDSiCCDS55752.1. [Q9NZC4-3]
CCDS55753.1. [Q9NZC4-2]
CCDS7894.1. [Q9NZC4-1]
PIRiJC7115.
RefSeqiNP_001193544.1. NM_001206615.1. [Q9NZC4-2]
NP_001193545.1. NM_001206616.1. [Q9NZC4-3]
NP_036285.2. NM_012153.5. [Q9NZC4-1]
XP_005252917.1. XM_005252860.1. [Q9NZC4-1]
XP_005252919.1. XM_005252862.1. [Q9NZC4-2]
XP_011518286.1. XM_011519984.1. [Q9NZC4-1]
XP_011518287.1. XM_011519985.1. [Q9NZC4-1]
UniGeneiHs.653859.

Genome annotation databases

EnsembliENST00000257831; ENSP00000257831; ENSG00000135373. [Q9NZC4-1]
ENST00000450654; ENSP00000399733; ENSG00000135373. [Q9NZC4-2]
ENST00000530286; ENSP00000433508; ENSG00000135373. [Q9NZC4-1]
ENST00000531794; ENSP00000435835; ENSG00000135373. [Q9NZC4-3]
ENST00000533754; ENSP00000435837; ENSG00000135373. [Q9NZC4-1]
GeneIDi26298.
KEGGihsa:26298.
UCSCiuc001mvr.3. human. [Q9NZC4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF170583 mRNA. Translation: AAF06998.1.
AF124438 mRNA. Translation: AAD30990.1.
AF124439 mRNA. Translation: AAD30991.1.
AF212848 mRNA. Translation: AAF61670.1.
AF203977 mRNA. Translation: AAG35644.1.
AY882601 mRNA. Translation: AAW80658.1.
AK310867 mRNA. No translation available.
AC087783 Genomic DNA. No translation available.
AL157952 Genomic DNA. Translation: CAC12700.1.
AL157952 Genomic DNA. Translation: CAC12701.1.
CH471064 Genomic DNA. Translation: EAW68162.1.
CH471064 Genomic DNA. Translation: EAW68163.1.
CH471064 Genomic DNA. Translation: EAW68164.1.
CH471064 Genomic DNA. Translation: EAW68165.1.
CH471064 Genomic DNA. Translation: EAW68166.1.
BC038995 mRNA. Translation: AAH38995.1.
CCDSiCCDS55752.1. [Q9NZC4-3]
CCDS55753.1. [Q9NZC4-2]
CCDS7894.1. [Q9NZC4-1]
PIRiJC7115.
RefSeqiNP_001193544.1. NM_001206615.1. [Q9NZC4-2]
NP_001193545.1. NM_001206616.1. [Q9NZC4-3]
NP_036285.2. NM_012153.5. [Q9NZC4-1]
XP_005252917.1. XM_005252860.1. [Q9NZC4-1]
XP_005252919.1. XM_005252862.1. [Q9NZC4-2]
XP_011518286.1. XM_011519984.1. [Q9NZC4-1]
XP_011518287.1. XM_011519985.1. [Q9NZC4-1]
UniGeneiHs.653859.

3D structure databases

ProteinModelPortaliQ9NZC4.
SMRiQ9NZC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117675. 8 interactors.
IntActiQ9NZC4. 1 interactor.
MINTiMINT-8247463.
STRINGi9606.ENSP00000435835.

PTM databases

iPTMnetiQ9NZC4.
PhosphoSitePlusiQ9NZC4.

Polymorphism and mutation databases

BioMutaiEHF.
DMDMi74762761.

Proteomic databases

MaxQBiQ9NZC4.
PaxDbiQ9NZC4.
PeptideAtlasiQ9NZC4.
PRIDEiQ9NZC4.

Protocols and materials databases

DNASUi26298.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257831; ENSP00000257831; ENSG00000135373. [Q9NZC4-1]
ENST00000450654; ENSP00000399733; ENSG00000135373. [Q9NZC4-2]
ENST00000530286; ENSP00000433508; ENSG00000135373. [Q9NZC4-1]
ENST00000531794; ENSP00000435835; ENSG00000135373. [Q9NZC4-3]
ENST00000533754; ENSP00000435837; ENSG00000135373. [Q9NZC4-1]
GeneIDi26298.
KEGGihsa:26298.
UCSCiuc001mvr.3. human. [Q9NZC4-1]

Organism-specific databases

CTDi26298.
DisGeNETi26298.
GeneCardsiEHF.
HGNCiHGNC:3246. EHF.
HPAiHPA050923.
HPA060734.
MIMi605439. gene.
neXtProtiNX_Q9NZC4.
OpenTargetsiENSG00000135373.
PharmGKBiPA27681.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOVERGENiHBG074143.
InParanoidiQ9NZC4.
KOiK17102.
OMAiPFQEFDV.
OrthoDBiEOG091G0BE6.
PhylomeDBiQ9NZC4.
TreeFamiTF318679.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000135373-MONOMER.

Miscellaneous databases

ChiTaRSiEHF. human.
GeneWikiiEHF_(gene).
GenomeRNAii26298.
PROiQ9NZC4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000135373.
CleanExiHS_EHF.
ExpressionAtlasiQ9NZC4. baseline and differential.
GenevisibleiQ9NZC4. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033071. EHF.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF171. PTHR11849:SF171. 2 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEHF_HUMAN
AccessioniPrimary (citable) accession number: Q9NZC4
Secondary accession number(s): D3DR08
, D3DR10, E9PSB2, Q9H509, Q9UKF9, Q9Y5V4, Q9Y5V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.