Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9NZB2 (F120A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Constitutive coactivator of PPAR-gamma-like protein 1
Alternative name(s):
Oxidative stress-associated Src activator
Protein FAM120A
Gene names
Name:FAM120A
Synonyms:C9orf10, KIAA0183, OSSA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1118 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May participate in mRNA transport in the cytoplasm By similarity. Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses. Ref.10

Subunit structure

Interacts with PURA By similarity. Interacts with YES1, SRC, FYN. Upon tyrosine phosphorylation, interacts with PIK3R1. Ref.10

Subcellular location

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Translocates to plasma membrane upon ultraviolet exposure. Ref.10

Tissue specificity

Ubiquitously expressed. Highly expressed in scirrhous-type gastric cancer tissues compared with normal gastric mucosa (at protein level). Ref.6 Ref.10

Post-translational modification

Arg-982 is dimethylated, probably to asymmetric dimethylarginine.

Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure.

Sequence similarities

Belongs to the constitutive coactivator of PPAR-gamma family.

Sequence caution

The sequence AAF72866.1 differs from that shown. Reason: Frameshift at position 1055.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasm
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DiseaseTumor suppressor
   LigandRNA-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9NZB2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q9NZB2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     579-628: GEIKIAVSIE...SRKKTERLAF → VLSKGPWSGF...KFTFPFLNIE
     629-1118: Missing.
Isoform D (identifier: Q9NZB2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     890-935: Missing.
Isoform E (identifier: Q9NZB2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     637-651: FSPVIIKEWAAYKGK → CMYCNNPLFVFLGTS
     652-1118: Missing.
Isoform F (identifier: Q9NZB2-6)

The sequence of this isoform differs from the canonical sequence as follows:
     473-473: N → KPFQLYLQKNFVFHKENSIVLCSRILRHG

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11181118Constitutive coactivator of PPAR-gamma-like protein 1
PRO_0000221627

Regions

Region339 – 40567Interaction with YES1, SRC and FYN
Region829 – 1118290RNA binding

Amino acid modifications

Modified residue9321N6-acetyllysine By similarity
Modified residue10231Phosphoserine Ref.8
Modified residue10441Phosphoserine Ref.11
Modified residue10451Phosphoserine Ref.11
Modified residue10481Phosphoserine Ref.11

Natural variations

Alternative sequence4731N → KPFQLYLQKNFVFHKENSIV LCSRILRHG in isoform F.
VSP_036324
Alternative sequence579 – 62850GEIKI…ERLAF → VLSKGPWSGFCYLMSGHSYG CFVLLSFFEPFFCLTNLLET KFTFPFLNIE in isoform B.
VSP_004147
Alternative sequence629 – 1118490Missing in isoform B.
VSP_004148
Alternative sequence637 – 65115FSPVI…AYKGK → CMYCNNPLFVFLGTS in isoform E.
VSP_017278
Alternative sequence652 – 1118467Missing in isoform E.
VSP_017279
Alternative sequence890 – 93546Missing in isoform D.
VSP_017280
Natural variant3271Y → H.
Corresponds to variant rs11541747 [ dbSNP | Ensembl ].
VAR_054400

Experimental info

Sequence conflict517 – 5204EGKG → DSRR in AAF72867. Ref.1
Sequence conflict5561L → V in AAF72867. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: C9171EA01C8D17A0

FASTA1,118121,888
        10         20         30         40         50         60 
MGVQGFQDYI EKHCPSAVVP VELQKLARGS LVGGGRQRPP QTPLRLLVDA DNCLHRLYGG 

        70         80         90        100        110        120 
FYTDWVSGGQ WNHMLGYLAA LAKACFGGNI ELFVFFNGAL EKARLHEWVK RQGNERQTAQ 

       130        140        150        160        170        180 
QIVSHVQNKG TPPPKVWFLP PVCMAHCIRL ALIRFHVKVA QSIEDHHQEV IGFCRENGFH 

       190        200        210        220        230        240 
GLVAYDSDYA LCNIPYYFSA HALKLSRNGK SLTTSQYLMH EVAKQLDLNP NRFPIFAALL 

       250        260        270        280        290        300 
GNHILPDEDL ASFHWSLLGP EHPLASLKVR AHQLVLPPCD VVIKAVADYV RNIQDTSDLD 

       310        320        330        340        350        360 
AIAKDVFQHS QSRTDDKVIR FKRAIGYYSA TSKPMSFHPP HYLAARPGPF GMPGMVPPHV 

       370        380        390        400        410        420 
PPQMLNIPQT SLQAKPVAPQ VPSPGGAPGQ GPYPYSLSEP APLTLDTSGK NLTEQNSYSN 

       430        440        450        460        470        480 
IPHEGKHTPL YERSSPINPA QSGSPNHVDS AYFPGSSTSS SSDNDEGSGG ATNHISGNKI 

       490        500        510        520        530        540 
GWEKTGSHSE PQARGDPGDQ TKAEGSSTAS SGSQLAEGKG SQMGTVQPIP CLLSMPTRNH 

       550        560        570        580        590        600 
MDITTPPLPP VAPEVLRVAE HRHKKGLMYP YIFHVLTKGE IKIAVSIEDE ANKDLPPAAL 

       610        620        630        640        650        660 
LYRPVRQYVY GVLFSLAESR KKTERLAFRK NRLPPEFSPV IIKEWAAYKG KSPQTPELVE 

       670        680        690        700        710        720 
ALAFREWTCP NLKRLWLGKA VEDKNRRMRA FLACMRSDTP AMLNPANVPT HLMVLCCVLR 

       730        740        750        760        770        780 
YMVQWPGARI LRRQELDAFL AQALSPKLYE PDQLQELKIE NLDPRGIQLS ALFMSGVDMA 

       790        800        810        820        830        840 
LFANDACGQP IPWEHCCPWM YFDGKLFQSK LLKASREKTP LIDLCDGQAD QAAKVEKMRQ 

       850        860        870        880        890        900 
SVLEGLSFSR QSHTLPFPPP PALPFYPASA YPRHFGPVPP SQGRGRGFAG VCGFGGPYGE 

       910        920        930        940        950        960 
TVATGPYRAF RVAAASGHCG AFSGSDSSRT SKSQGGVQPI PSQGGKLEIA GTVVGHWAGS 

       970        980        990       1000       1010       1020 
RRGRGGRGPF PLQVVSVGGP ARGRPRGVIS TPVIRTFGRG GRYYGRGYKN QAAIQGRPPY 

      1030       1040       1050       1060       1070       1080 
AASAEEVAKE LKSKSGESKS SAMSSDGSLA ENGVMAEEKP APQMNGSTGD ARAPSHSESA 

      1090       1100       1110 
LNNDSKTCNT NPHLNALSTD SACRREAALE AAVLNKEE 

« Hide

Isoform B [UniParc].

Checksum: 4C39CFE952A4A18D
Show »

FASTA62868,993
Isoform D [UniParc].

Checksum: 591F2B96B0E4DB49
Show »

FASTA1,072117,406
Isoform E [UniParc].

Checksum: 013F2DADA8C3008E
Show »

FASTA65171,561
Isoform F [UniParc].

Checksum: 3E79D081CBABBD9C
Show »

FASTA1,146125,272

References

« Hide 'large scale' references
[1]"Generating full-length coding sequence for 2 alternate transcripts of a novel gene C9orf10 and screening HSN-I patients for mutations."
Brahmbhatt S.B., Hulme D.J., Dawkins J.L., Nicholson G.A.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), NUCLEOTIDE SEQUENCE [MRNA] OF 1-1102 (ISOFORM A).
[2]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-1118 (ISOFORM E).
Tissue: Lung and Placenta.
[4]"Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1."
Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N.
DNA Res. 3:17-24(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-1069 (ISOFORM A).
Tissue: Bone marrow.
[5]Ohara O., Nagase T., Kikuno R., Nomura N.
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[6]"The human gene CXorf17 encodes a member of a novel family of putative transmembrane proteins: cDNA cloning and characterization of CXorf17 and its mouse ortholog orf34."
Holden S., Raymond F.L.
Gene 318:149-161(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 393-512 (ISOFORM F), TISSUE SPECIFICITY.
[7]Yu W., Gibbs R.A.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 579-1069 (ISOFORM D).
Tissue: Brain.
[8]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1023, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A novel RNA-binding protein, Ossa/C9orf10, regulates activity of Src kinases to protect cells from oxidative stress-induced apoptosis."
Tanaka M., Sasaki K., Kamata R., Hoshino Y., Yanagihara K., Sakai R.
Mol. Cell. Biol. 29:402-413(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH YES1; SRC AND FYN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1044; SER-1045 AND SER-1048, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF214737 mRNA. Translation: AAF72866.1. Frameshift.
AF214738 mRNA. Translation: AAF72867.1.
AL353629 Genomic DNA. Translation: CAI96105.1.
AL353629 Genomic DNA. Translation: CAI96106.1.
AL353629 Genomic DNA. Translation: CAI96108.1.
AL353629 Genomic DNA. Translation: CAI96109.1.
BC007879 mRNA. Translation: AAH07879.2.
BC098584 mRNA. Translation: AAH98584.1.
BC111736 mRNA. Translation: AAI11737.1.
D80005 mRNA. Translation: BAA11500.2.
AY266457 mRNA. Translation: AAP31031.1.
AF055017 mRNA. Translation: AAC09364.1.
RefSeqNP_001273651.1. NM_001286722.1.
NP_001273652.1. NM_001286723.1.
NP_001273653.1. NM_001286724.1.
NP_055427.2. NM_014612.4.
UniGeneHs.372003.
Hs.707324.
Hs.738743.

3D structure databases

ProteinModelPortalQ9NZB2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116805. 10 interactions.
IntActQ9NZB2. 8 interactions.
MINTMINT-4090817.

PTM databases

PhosphoSiteQ9NZB2.

Polymorphism databases

DMDM158523294.

Proteomic databases

PaxDbQ9NZB2.
PRIDEQ9NZB2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000277165; ENSP00000277165; ENSG00000048828. [Q9NZB2-1]
ENST00000333936; ENSP00000334918; ENSG00000048828. [Q9NZB2-6]
ENST00000340893; ENSP00000344698; ENSG00000048828. [Q9NZB2-4]
ENST00000375389; ENSP00000364538; ENSG00000048828. [Q9NZB2-2]
ENST00000427765; ENSP00000412440; ENSG00000048828.
ENST00000446420; ENSP00000396534; ENSG00000048828.
GeneID23196.
KEGGhsa:23196.
UCSCuc004atv.3. human. [Q9NZB2-2]
uc004atw.3. human. [Q9NZB2-1]

Organism-specific databases

CTD23196.
GeneCardsGC09P096214.
HGNCHGNC:13247. FAM120A.
HPAHPA019734.
HPA055800.
MIM612265. gene.
neXtProtNX_Q9NZB2.
PharmGKBPA134954136.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG40155.
HOVERGENHBG050957.
InParanoidQ9NZB2.
OMAEKPAPQM.
OrthoDBEOG7G4QD9.
PhylomeDBQ9NZB2.
TreeFamTF328642.

Gene expression databases

BgeeQ9NZB2.
GenevestigatorQ9NZB2.

Family and domain databases

InterProIPR026783. Coact_PPARg-like_1.
[Graphical view]
PANTHERPTHR15976:SF4. PTHR15976:SF4. 1 hit.
ProtoNetSearch...

Other

ChiTaRSFAM120A. human.
GeneWikiFAM120A.
GenomeRNAi23196.
NextBio44699.
PROQ9NZB2.
SOURCESearch...

Entry information

Entry nameF120A_HUMAN
AccessionPrimary (citable) accession number: Q9NZB2
Secondary accession number(s): A6NGU0 expand/collapse secondary AC list , C4AMC6, O60649, Q14688, Q4VXF4, Q4VXF5, Q4VXG2, Q86V69, Q96I21, Q9NZB1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM