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Q9NZ53 (PDXL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Podocalyxin-like protein 2
Alternative name(s):
Endoglycan
Gene names
Name:PODXL2
ORF Names:UNQ1861/PRO3742
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins. Ref.6

Subunit structure

Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP. Ref.4 Ref.6

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells. Ref.1 Ref.6

Post-translational modification

Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides. Ref.1 Ref.4 Ref.6 Ref.9

Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.

Sequence similarities

Belongs to the podocalyxin family.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   LigandSialic acid
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
Sulfation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleukocyte tethering or rolling

Inferred from direct assay Ref.6. Source: UniProtKB

   Cellular_componentintegral component of plasma membrane

Traceable author statement Ref.1. Source: ProtInc

   Molecular_functionglycosaminoglycan binding

Traceable author statement Ref.1. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NZ53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NZ53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     336-411: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 605573Podocalyxin-like protein 2
PRO_0000252129

Regions

Topological domain33 – 500468Extracellular Potential
Transmembrane501 – 52121Helical; Potential
Topological domain522 – 60584Cytoplasmic Potential
Region129 – 1346O-glycosylated at one site
Compositional bias162 – 19837Glu-rich

Amino acid modifications

Modified residue971Sulfotyrosine Ref.4
Modified residue1181Sulfotyrosine Ref.4
Modified residue5701Phosphoserine Ref.8
Modified residue5961Phosphoserine Ref.5 Ref.7 Ref.8
Glycosylation1441O-linked (GalNAc...) Ref.9
Glycosylation1931N-linked (GlcNAc...) Potential
Glycosylation3951N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence336 – 41176Missing in isoform 2.
VSP_020876
Natural variant4561V → A.
Corresponds to variant rs34117815 [ dbSNP | Ensembl ].
VAR_053599

Experimental info

Mutagenesis971Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-118. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-118 and A-124. Ref.4
Mutagenesis1181Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-97. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-97 and A-124. Ref.4
Mutagenesis1241T → A: Not sialylated O-linked. Ref.4
Sequence conflict771P → S in AAQ89454. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 2F9B8DC51F7FC22A

FASTA60565,076
        10         20         30         40         50         60 
MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL LLPTGLEPLD 

        70         80         90        100        110        120 
SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE EEEELNDSSL DLGPTADYVF 

       130        140        150        160        170        180 
PDLTEKAGSI EDTSQAQELP NLPSPLPKMN LVEPPWHMPP REEEEEEEEE EEREKEEVEK 

       190        200        210        220        230        240 
QEEEEEEELL PVNGSQEEAK PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG 

       250        260        270        280        290        300 
PSLLLPSVTP TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH 

       310        320        330        340        350        360 
EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPLAPGD MELTPSSATL GQEDLNQQLL 

       370        380        390        400        410        420 
EGQAAEAQSR IPWDSTQVIC KDWSNLAGKN YIILNMTENI DCEVFRQHRG PQLLALVEEV 

       430        440        450        460        470        480 
LPRHGSGHHG AWHISLSKPS EKEQHLLMTL VGEQGVVPTQ DVLSMLGDIR RSLEEIGIQN 

       490        500        510        520        530        540 
YSTTSSCQAR ASQVRSDYGT LFVVLVVIGA ICIIIIALGL LYNCWQRRLP KLKHVSHGEE 

       550        560        570        580        590        600 
LRFVENGCHD NPTLDVASDS QSEMQEKHPS LNGGGALNGP GSWGALMGGK RDPEDSDVFE 


EDTHL 

« Hide

Isoform 2 [UniParc].

Checksum: 210B6021D61EC8C0
Show »

FASTA52956,635

References

« Hide 'large scale' references
[1]"Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins."
Sassetti C., Van Zante A., Rosen S.D.
J. Biol. Chem. 275:9001-9010(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, GLYCOSYLATION.
Tissue: Brain.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Melanoma and Neuroblastoma.
[4]"Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x."
Fieger C.B., Sassetti C.M., Rosen S.D.
J. Biol. Chem. 278:27390-27398(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SELL, SUBUNIT, SULFATION AT TYR-97 AND TYR-118, GLYCOSYLATION, SIALIC ACID CONTENT, MUTAGENESIS OF TYR-97; TYR-118 AND THR-124.
[5]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[6]"Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins."
Kerr S.C., Fieger C.B., Snapp K.R., Rosen S.D.
J. Immunol. 181:1480-1490(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SELL; SELE AND SELP, GLYCOSYLATION, TISSUE SPECIFICITY.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-570 AND SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
Halim A., Ruetschi U., Larson G., Nilsson J.
J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF219137 mRNA. Translation: AAF44629.1.
AY359096 mRNA. Translation: AAQ89454.1.
BC019330 mRNA. Translation: AAH19330.1.
BC052585 mRNA. Translation: AAH52585.1.
RefSeqNP_056535.1. NM_015720.3.
UniGeneHs.591290.

3D structure databases

ProteinModelPortalQ9NZ53.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119082. 3 interactions.
IntActQ9NZ53. 1 interaction.
STRING9606.ENSP00000345359.

PTM databases

PhosphoSiteQ9NZ53.

Polymorphism databases

DMDM74734719.

Proteomic databases

PaxDbQ9NZ53.
PRIDEQ9NZ53.

Protocols and materials databases

DNASU50512.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000342480; ENSP00000345359; ENSG00000114631. [Q9NZ53-1]
GeneID50512.
KEGGhsa:50512.
UCSCuc003ejq.3. human. [Q9NZ53-1]

Organism-specific databases

CTD50512.
GeneCardsGC03P127348.
HGNCHGNC:17936. PODXL2.
HPACAB024934.
HPA042265.
neXtProtNX_Q9NZ53.
PharmGKBPA134860950.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43739.
HOGENOMHOG000054210.
HOVERGENHBG082114.
InParanoidQ9NZ53.
KOK06818.
OMAIPWDSTQ.
OrthoDBEOG72C51N.
PhylomeDBQ9NZ53.
TreeFamTF333564.

Gene expression databases

BgeeQ9NZ53.
CleanExHS_PODXL2.
GenevestigatorQ9NZ53.

Family and domain databases

InterProIPR013836. CD34/Podocalyxin.
[Graphical view]
PfamPF06365. CD34_antigen. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi50512.
NextBio53086.
PROQ9NZ53.

Entry information

Entry namePDXL2_HUMAN
AccessionPrimary (citable) accession number: Q9NZ53
Secondary accession number(s): Q6UVY4, Q8WUV6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM