Q9NZ53 (PDXL2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Podocalyxin-like protein 2

Alternative name(s):
Endoglycan

Gene names

Name:	PODXL2
ORF Names:	UNQ1861/PRO3742

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins. Ref.6
Subunit structure	Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP. Ref.4 Ref.6
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells. Ref.1 Ref.6
Post-translational modification	Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides. Ref.1 Ref.4 Ref.6 Ref.9 Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.
Sequence similarities	Belongs to the podocalyxin family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Ligand	Sialic acid
PTM	Disulfide bond Glycoprotein Phosphoprotein Sulfation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	leukocyte tethering or rolling Inferred from direct assay Ref.6. Source: UniProtKB
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	glycosaminoglycan binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NZ53-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NZ53-2)

The sequence of this isoform differs from the canonical sequence as follows:
     336-411: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	Potential
	Chain	33 – 605	573	Podocalyxin-like protein 2		PRO_0000252129
Regions
	Topological domain	33 – 500	468	Extracellular Potential
	Transmembrane	501 – 521	21	Helical; Potential
	Topological domain	522 – 605	84	Cytoplasmic Potential
	Region	129 – 134	6	O-glycosylated at one site
	Compositional bias	162 – 198	37	Glu-rich
Amino acid modifications
	Modified residue	97	1	Sulfotyrosine Ref.4
	Modified residue	118	1	Sulfotyrosine Ref.4
	Modified residue	570	1	Phosphoserine Ref.8
	Modified residue	596	1	Phosphoserine Ref.5 Ref.7 Ref.8
	Glycosylation	144	1	O-linked (GalNAc...) Ref.9
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	395	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	336 – 411	76	Missing in isoform 2.		VSP_020876
	Natural variant	456	1	V → A. Corresponds to variant rs34117815 [ dbSNP | Ensembl ].		VAR_053599
Experimental info
	Mutagenesis	97	1	Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-118. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-118 and A-124. Ref.4
	Mutagenesis	118	1	Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-97. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-97 and A-124. Ref.4
	Mutagenesis	124	1	T → A: Not sialylated O-linked. Ref.4
	Sequence conflict	77	1	P → S in AAQ89454. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2F9B8DC51F7FC22A	FASTA	605	65,076
	10 20 30 40 50 60 MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL LLPTGLEPLD 70 80 90 100 110 120 SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE EEEELNDSSL DLGPTADYVF 130 140 150 160 170 180 PDLTEKAGSI EDTSQAQELP NLPSPLPKMN LVEPPWHMPP REEEEEEEEE EEREKEEVEK 190 200 210 220 230 240 QEEEEEEELL PVNGSQEEAK PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG 250 260 270 280 290 300 PSLLLPSVTP TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH 310 320 330 340 350 360 EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPLAPGD MELTPSSATL GQEDLNQQLL 370 380 390 400 410 420 EGQAAEAQSR IPWDSTQVIC KDWSNLAGKN YIILNMTENI DCEVFRQHRG PQLLALVEEV 430 440 450 460 470 480 LPRHGSGHHG AWHISLSKPS EKEQHLLMTL VGEQGVVPTQ DVLSMLGDIR RSLEEIGIQN 490 500 510 520 530 540 YSTTSSCQAR ASQVRSDYGT LFVVLVVIGA ICIIIIALGL LYNCWQRRLP KLKHVSHGEE 550 560 570 580 590 600 LRFVENGCHD NPTLDVASDS QSEMQEKHPS LNGGGALNGP GSWGALMGGK RDPEDSDVFE EDTHL
	Isoform 2 [UniParc]. Checksum: 210B6021D61EC8C0	FASTA	529	56,635
	10 20 30 40 50 60 MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL LLPTGLEPLD 70 80 90 100 110 120 SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE EEEELNDSSL DLGPTADYVF 130 140 150 160 170 180 PDLTEKAGSI EDTSQAQELP NLPSPLPKMN LVEPPWHMPP REEEEEEEEE EEREKEEVEK 190 200 210 220 230 240 QEEEEEEELL PVNGSQEEAK PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG 250 260 270 280 290 300 PSLLLPSVTP TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH 310 320 330 340 350 360 EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPQLLAL VEEVLPRHGS GHHGAWHISL 370 380 390 400 410 420 SKPSEKEQHL LMTLVGEQGV VPTQDVLSML GDIRRSLEEI GIQNYSTTSS CQARASQVRS 430 440 450 460 470 480 DYGTLFVVLV VIGAICIIII ALGLLYNCWQ RRLPKLKHVS HGEELRFVEN GCHDNPTLDV 490 500 510 520 ASDSQSEMQE KHPSLNGGGA LNGPGSWGAL MGGKRDPEDS DVFEEDTHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins." Sassetti C., Van Zante A., Rosen S.D. J. Biol. Chem. 275:9001-9010(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, GLYCOSYLATION. Tissue: Brain.
[2]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Melanoma and Neuroblastoma.
[4]	"Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x." Fieger C.B., Sassetti C.M., Rosen S.D. J. Biol. Chem. 278:27390-27398(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SELL, SUBUNIT, SULFATION AT TYR-97 AND TYR-118, GLYCOSYLATION, SIALIC ACID CONTENT, MUTAGENESIS OF TYR-97; TYR-118 AND THR-124.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins." Kerr S.C., Fieger C.B., Snapp K.R., Rosen S.D. J. Immunol. 181:1480-1490(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SELL; SELE AND SELP, GLYCOSYLATION, TISSUE SPECIFICITY.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-570 AND SER-596, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins." Halim A., Ruetschi U., Larson G., Nilsson J. J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT SER-144, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF219137 mRNA. Translation: AAF44629.1. AY359096 mRNA. Translation: AAQ89454.1. BC019330 mRNA. Translation: AAH19330.1. BC052585 mRNA. Translation: AAH52585.1.
IPI	IPI00419595. IPI00978826.
RefSeq	NP_056535.1. NM_015720.3.
UniGene	Hs.591290.
3D structure databases
ProteinModelPortal	Q9NZ53.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NZ53. 1 interaction.
STRING	9606.ENSP00000345359.
PTM databases
PhosphoSite	Q9NZ53.
Polymorphism databases
DMDM	74734719.
Proteomic databases
PaxDb	Q9NZ53.
PRIDE	Q9NZ53.
Protocols and materials databases
DNASU	50512.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000342480; ENSP00000345359; ENSG00000114631.
GeneID	50512.
KEGG	hsa:50512.
UCSC	uc003ejq.2. human.
Organism-specific databases
CTD	50512.
GeneCards	GC03P127348.
HGNC	HGNC:17936. PODXL2.
HPA	CAB024934.
neXtProt	NX_Q9NZ53.
PharmGKB	PA134860950.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG43739.
HOGENOM	HOG000054210.
HOVERGEN	HBG082114.
InParanoid	Q9NZ53.
KO	K06818.
OMA	FPSEESE.
OrthoDB	EOG4TMR1Z.
PhylomeDB	Q9NZ53.
Gene expression databases
Bgee	Q9NZ53.
CleanEx	HS_PODXL2.
Genevestigator	Q9NZ53.
GermOnline	ENSG00000114631. Homo sapiens.
Family and domain databases
InterPro	IPR013836. CD34/Podocalyxin. [Graphical view]
Pfam	PF06365. CD34_antigen. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	50512.
NextBio	53086.

Entry information

Entry name

PDXL2_HUMAN

Accession

Primary (citable) accession number: Q9NZ53
Secondary accession number(s): Q6UVY4, Q8WUV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.