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Protein

Podocalyxin-like protein 2

Gene

PODXL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.1 Publication

GO - Molecular functioni

  • glycosaminoglycan binding Source: ProtInc

GO - Biological processi

  • leukocyte tethering or rolling Source: UniProtKB

Keywordsi

Biological processCell adhesion
LigandSialic acid

Enzyme and pathway databases

ReactomeiR-HSA-156584 Cytosolic sulfonation of small molecules

Names & Taxonomyi

Protein namesi
Recommended name:
Podocalyxin-like protein 2
Alternative name(s):
Endoglycan
Gene namesi
Name:PODXL2
ORF Names:UNQ1861/PRO3742
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000114631.10
HGNCiHGNC:17936 PODXL2
MIMi616627 gene
neXtProtiNX_Q9NZ53

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 500ExtracellularSequence analysisAdd BLAST468
Transmembranei501 – 521HelicalSequence analysisAdd BLAST21
Topological domaini522 – 605CytoplasmicSequence analysisAdd BLAST84

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-118. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-118 and A-124. 1 Publication1
Mutagenesisi118Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-97. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-97 and A-124. 1 Publication1
Mutagenesisi124T → A: Not sialylated O-linked. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000114631
PharmGKBiPA134860950

Polymorphism and mutation databases

DMDMi74734719

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000025212933 – 605Podocalyxin-like protein 2Add BLAST573

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97Sulfotyrosine1 Publication1
Modified residuei118Sulfotyrosine1 Publication1
Glycosylationi144O-linked (GalNAc...) serine1 Publication1
Glycosylationi193N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi395N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei570PhosphoserineCombined sources1
Modified residuei596PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides.1 Publication
Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

EPDiQ9NZ53
MaxQBiQ9NZ53
PaxDbiQ9NZ53
PeptideAtlasiQ9NZ53
PRIDEiQ9NZ53

PTM databases

GlyConnecti687
iPTMnetiQ9NZ53
PhosphoSitePlusiQ9NZ53
UniCarbKBiQ9NZ53

Expressioni

Tissue specificityi

Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.2 Publications

Gene expression databases

BgeeiENSG00000114631
CleanExiHS_PODXL2
GenevisibleiQ9NZ53 HS

Organism-specific databases

HPAiCAB024934
HPA042265

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP.2 Publications

Protein-protein interaction databases

BioGridi119082, 22 interactors
IntActiQ9NZ53, 1 interactor
STRINGi9606.ENSP00000345359

Structurei

3D structure databases

ProteinModelPortaliQ9NZ53
SMRiQ9NZ53
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 134O-glycosylated at one site6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 198Glu-richAdd BLAST37

Sequence similaritiesi

Belongs to the podocalyxin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFKB Eukaryota
ENOG410YCG4 LUCA
GeneTreeiENSGT00730000111323
HOGENOMiHOG000054210
HOVERGENiHBG082114
InParanoidiQ9NZ53
KOiK06818
OMAiIPWDSTQ
OrthoDBiEOG091G04AY
PhylomeDBiQ9NZ53
TreeFamiTF333564

Family and domain databases

InterProiView protein in InterPro
IPR013836 CD34/Podocalyxin
PfamiView protein in Pfam
PF06365 CD34_antigen, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZ53-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL
60 70 80 90 100
LLPTGLEPLD SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE
110 120 130 140 150
EEEELNDSSL DLGPTADYVF PDLTEKAGSI EDTSQAQELP NLPSPLPKMN
160 170 180 190 200
LVEPPWHMPP REEEEEEEEE EEREKEEVEK QEEEEEEELL PVNGSQEEAK
210 220 230 240 250
PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG PSLLLPSVTP
260 270 280 290 300
TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH
310 320 330 340 350
EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPLAPGD MELTPSSATL
360 370 380 390 400
GQEDLNQQLL EGQAAEAQSR IPWDSTQVIC KDWSNLAGKN YIILNMTENI
410 420 430 440 450
DCEVFRQHRG PQLLALVEEV LPRHGSGHHG AWHISLSKPS EKEQHLLMTL
460 470 480 490 500
VGEQGVVPTQ DVLSMLGDIR RSLEEIGIQN YSTTSSCQAR ASQVRSDYGT
510 520 530 540 550
LFVVLVVIGA ICIIIIALGL LYNCWQRRLP KLKHVSHGEE LRFVENGCHD
560 570 580 590 600
NPTLDVASDS QSEMQEKHPS LNGGGALNGP GSWGALMGGK RDPEDSDVFE

EDTHL
Length:605
Mass (Da):65,076
Last modified:October 1, 2000 - v1
Checksum:i2F9B8DC51F7FC22A
GO
Isoform 2 (identifier: Q9NZ53-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-411: Missing.

Note: No experimental confirmation available.
Show »
Length:529
Mass (Da):56,635
Checksum:i210B6021D61EC8C0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77P → S in AAQ89454 (PubMed:12975309).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053599456V → A. Corresponds to variant dbSNP:rs34117815Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020876336 – 411Missing in isoform 2. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF219137 mRNA Translation: AAF44629.1
AY359096 mRNA Translation: AAQ89454.1
BC019330 mRNA Translation: AAH19330.1
BC052585 mRNA Translation: AAH52585.1
CCDSiCCDS3044.1 [Q9NZ53-1]
RefSeqiNP_056535.1, NM_015720.3 [Q9NZ53-1]
UniGeneiHs.591290

Genome annotation databases

EnsembliENST00000342480; ENSP00000345359; ENSG00000114631 [Q9NZ53-1]
GeneIDi50512
KEGGihsa:50512
UCSCiuc003ejq.4 human [Q9NZ53-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPDXL2_HUMAN
AccessioniPrimary (citable) accession number: Q9NZ53
Secondary accession number(s): Q6UVY4, Q8WUV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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