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Protein

Podocalyxin-like protein 2

Gene

PODXL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.1 Publication

GO - Molecular functioni

  • glycosaminoglycan binding Source: ProtInc

GO - Biological processi

  • leukocyte tethering or rolling Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Podocalyxin-like protein 2
Alternative name(s):
Endoglycan
Gene namesi
Name:PODXL2
ORF Names:UNQ1861/PRO3742
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17936. PODXL2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini33 – 500ExtracellularSequence analysisAdd BLAST468
Transmembranei501 – 521HelicalSequence analysisAdd BLAST21
Topological domaini522 – 605CytoplasmicSequence analysisAdd BLAST84

GO - Cellular componenti

  • integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-118. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-118 and A-124. 1 Publication1
Mutagenesisi118Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-97. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-97 and A-124. 1 Publication1
Mutagenesisi124T → A: Not sialylated O-linked. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000114631.
PharmGKBiPA134860950.

Polymorphism and mutation databases

DMDMi74734719.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000025212933 – 605Podocalyxin-like protein 2Add BLAST573

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97Sulfotyrosine1 Publication1
Modified residuei118Sulfotyrosine1 Publication1
Glycosylationi144O-linked (GalNAc...)1 Publication1
Glycosylationi193N-linked (GlcNAc...)Sequence analysis1
Glycosylationi395N-linked (GlcNAc...)Sequence analysis1
Modified residuei570PhosphoserineCombined sources1
Modified residuei596PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides.1 Publication
Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

EPDiQ9NZ53.
MaxQBiQ9NZ53.
PaxDbiQ9NZ53.
PeptideAtlasiQ9NZ53.
PRIDEiQ9NZ53.

PTM databases

iPTMnetiQ9NZ53.
PhosphoSitePlusiQ9NZ53.
UniCarbKBiQ9NZ53.

Expressioni

Tissue specificityi

Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.2 Publications

Gene expression databases

BgeeiENSG00000114631.
CleanExiHS_PODXL2.
GenevisibleiQ9NZ53. HS.

Organism-specific databases

HPAiCAB024934.
HPA042265.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP.2 Publications

Protein-protein interaction databases

BioGridi119082. 14 interactors.
IntActiQ9NZ53. 1 interactor.
STRINGi9606.ENSP00000345359.

Structurei

3D structure databases

ProteinModelPortaliQ9NZ53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 134O-glycosylated at one site6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 198Glu-richAdd BLAST37

Sequence similaritiesi

Belongs to the podocalyxin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFKB. Eukaryota.
ENOG410YCG4. LUCA.
GeneTreeiENSGT00730000111323.
HOGENOMiHOG000054210.
HOVERGENiHBG082114.
InParanoidiQ9NZ53.
KOiK06818.
OMAiIPWDSTQ.
OrthoDBiEOG091G04AY.
PhylomeDBiQ9NZ53.
TreeFamiTF333564.

Family and domain databases

InterProiIPR013836. CD34/Podocalyxin.
[Graphical view]
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NZ53-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL
60 70 80 90 100
LLPTGLEPLD SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE
110 120 130 140 150
EEEELNDSSL DLGPTADYVF PDLTEKAGSI EDTSQAQELP NLPSPLPKMN
160 170 180 190 200
LVEPPWHMPP REEEEEEEEE EEREKEEVEK QEEEEEEELL PVNGSQEEAK
210 220 230 240 250
PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG PSLLLPSVTP
260 270 280 290 300
TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH
310 320 330 340 350
EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPLAPGD MELTPSSATL
360 370 380 390 400
GQEDLNQQLL EGQAAEAQSR IPWDSTQVIC KDWSNLAGKN YIILNMTENI
410 420 430 440 450
DCEVFRQHRG PQLLALVEEV LPRHGSGHHG AWHISLSKPS EKEQHLLMTL
460 470 480 490 500
VGEQGVVPTQ DVLSMLGDIR RSLEEIGIQN YSTTSSCQAR ASQVRSDYGT
510 520 530 540 550
LFVVLVVIGA ICIIIIALGL LYNCWQRRLP KLKHVSHGEE LRFVENGCHD
560 570 580 590 600
NPTLDVASDS QSEMQEKHPS LNGGGALNGP GSWGALMGGK RDPEDSDVFE

EDTHL
Length:605
Mass (Da):65,076
Last modified:October 1, 2000 - v1
Checksum:i2F9B8DC51F7FC22A
GO
Isoform 2 (identifier: Q9NZ53-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-411: Missing.

Note: No experimental confirmation available.
Show »
Length:529
Mass (Da):56,635
Checksum:i210B6021D61EC8C0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77P → S in AAQ89454 (PubMed:12975309).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053599456V → A.Corresponds to variant rs34117815dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020876336 – 411Missing in isoform 2. 1 PublicationAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF219137 mRNA. Translation: AAF44629.1.
AY359096 mRNA. Translation: AAQ89454.1.
BC019330 mRNA. Translation: AAH19330.1.
BC052585 mRNA. Translation: AAH52585.1.
CCDSiCCDS3044.1. [Q9NZ53-1]
RefSeqiNP_056535.1. NM_015720.3. [Q9NZ53-1]
UniGeneiHs.591290.

Genome annotation databases

EnsembliENST00000342480; ENSP00000345359; ENSG00000114631. [Q9NZ53-1]
GeneIDi50512.
KEGGihsa:50512.
UCSCiuc003ejq.4. human. [Q9NZ53-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF219137 mRNA. Translation: AAF44629.1.
AY359096 mRNA. Translation: AAQ89454.1.
BC019330 mRNA. Translation: AAH19330.1.
BC052585 mRNA. Translation: AAH52585.1.
CCDSiCCDS3044.1. [Q9NZ53-1]
RefSeqiNP_056535.1. NM_015720.3. [Q9NZ53-1]
UniGeneiHs.591290.

3D structure databases

ProteinModelPortaliQ9NZ53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119082. 14 interactors.
IntActiQ9NZ53. 1 interactor.
STRINGi9606.ENSP00000345359.

PTM databases

iPTMnetiQ9NZ53.
PhosphoSitePlusiQ9NZ53.
UniCarbKBiQ9NZ53.

Polymorphism and mutation databases

DMDMi74734719.

Proteomic databases

EPDiQ9NZ53.
MaxQBiQ9NZ53.
PaxDbiQ9NZ53.
PeptideAtlasiQ9NZ53.
PRIDEiQ9NZ53.

Protocols and materials databases

DNASUi50512.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342480; ENSP00000345359; ENSG00000114631. [Q9NZ53-1]
GeneIDi50512.
KEGGihsa:50512.
UCSCiuc003ejq.4. human. [Q9NZ53-1]

Organism-specific databases

CTDi50512.
GeneCardsiPODXL2.
HGNCiHGNC:17936. PODXL2.
HPAiCAB024934.
HPA042265.
MIMi616627. gene.
neXtProtiNX_Q9NZ53.
OpenTargetsiENSG00000114631.
PharmGKBiPA134860950.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFKB. Eukaryota.
ENOG410YCG4. LUCA.
GeneTreeiENSGT00730000111323.
HOGENOMiHOG000054210.
HOVERGENiHBG082114.
InParanoidiQ9NZ53.
KOiK06818.
OMAiIPWDSTQ.
OrthoDBiEOG091G04AY.
PhylomeDBiQ9NZ53.
TreeFamiTF333564.

Miscellaneous databases

ChiTaRSiPODXL2. human.
GenomeRNAii50512.
PROiQ9NZ53.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114631.
CleanExiHS_PODXL2.
GenevisibleiQ9NZ53. HS.

Family and domain databases

InterProiIPR013836. CD34/Podocalyxin.
[Graphical view]
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDXL2_HUMAN
AccessioniPrimary (citable) accession number: Q9NZ53
Secondary accession number(s): Q6UVY4, Q8WUV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.