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Q9NZ53

- PDXL2_HUMAN

UniProt

Q9NZ53 - PDXL2_HUMAN

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Protein

Podocalyxin-like protein 2

Gene

PODXL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.1 Publication

GO - Molecular functioni

  1. glycosaminoglycan binding Source: ProtInc

GO - Biological processi

  1. leukocyte tethering or rolling Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Podocalyxin-like protein 2
Alternative name(s):
Endoglycan
Gene namesi
Name:PODXL2
ORF Names:UNQ1861/PRO3742
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:17936. PODXL2.

Subcellular locationi

GO - Cellular componenti

  1. integral component of plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi97 – 971Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-118. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-118 and A-124. 1 Publication
Mutagenesisi118 – 1181Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-97. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-97 and A-124. 1 Publication
Mutagenesisi124 – 1241T → A: Not sialylated O-linked. 1 Publication

Organism-specific databases

PharmGKBiPA134860950.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 605573Podocalyxin-like protein 2PRO_0000252129Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971Sulfotyrosine1 Publication
Modified residuei118 – 1181Sulfotyrosine1 Publication
Glycosylationi144 – 1441O-linked (GalNAc...)1 Publication
Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence Analysis
Modified residuei570 – 5701Phosphoserine1 Publication
Modified residuei596 – 5961Phosphoserine3 Publications

Post-translational modificationi

Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides.1 Publication
Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

MaxQBiQ9NZ53.
PaxDbiQ9NZ53.
PRIDEiQ9NZ53.

PTM databases

PhosphoSiteiQ9NZ53.

Expressioni

Tissue specificityi

Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.2 Publications

Gene expression databases

BgeeiQ9NZ53.
CleanExiHS_PODXL2.
GenevestigatoriQ9NZ53.

Organism-specific databases

HPAiCAB024934.
HPA042265.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP.2 Publications

Protein-protein interaction databases

BioGridi119082. 5 interactions.
IntActiQ9NZ53. 1 interaction.
STRINGi9606.ENSP00000345359.

Structurei

3D structure databases

ProteinModelPortaliQ9NZ53.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 500468ExtracellularSequence AnalysisAdd
BLAST
Topological domaini522 – 60584CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei501 – 52121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1346O-glycosylated at one site

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi162 – 19837Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the podocalyxin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43739.
GeneTreeiENSGT00730000111323.
HOGENOMiHOG000054210.
HOVERGENiHBG082114.
InParanoidiQ9NZ53.
KOiK06818.
OMAiIPWDSTQ.
OrthoDBiEOG72C51N.
PhylomeDBiQ9NZ53.
TreeFamiTF333564.

Family and domain databases

InterProiIPR013836. CD34/Podocalyxin.
[Graphical view]
PfamiPF06365. CD34_antigen. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NZ53-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL
60 70 80 90 100
LLPTGLEPLD SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE
110 120 130 140 150
EEEELNDSSL DLGPTADYVF PDLTEKAGSI EDTSQAQELP NLPSPLPKMN
160 170 180 190 200
LVEPPWHMPP REEEEEEEEE EEREKEEVEK QEEEEEEELL PVNGSQEEAK
210 220 230 240 250
PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG PSLLLPSVTP
260 270 280 290 300
TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH
310 320 330 340 350
EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPLAPGD MELTPSSATL
360 370 380 390 400
GQEDLNQQLL EGQAAEAQSR IPWDSTQVIC KDWSNLAGKN YIILNMTENI
410 420 430 440 450
DCEVFRQHRG PQLLALVEEV LPRHGSGHHG AWHISLSKPS EKEQHLLMTL
460 470 480 490 500
VGEQGVVPTQ DVLSMLGDIR RSLEEIGIQN YSTTSSCQAR ASQVRSDYGT
510 520 530 540 550
LFVVLVVIGA ICIIIIALGL LYNCWQRRLP KLKHVSHGEE LRFVENGCHD
560 570 580 590 600
NPTLDVASDS QSEMQEKHPS LNGGGALNGP GSWGALMGGK RDPEDSDVFE

EDTHL
Length:605
Mass (Da):65,076
Last modified:October 1, 2000 - v1
Checksum:i2F9B8DC51F7FC22A
GO
Isoform 2 (identifier: Q9NZ53-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-411: Missing.

Note: No experimental confirmation available.

Show »
Length:529
Mass (Da):56,635
Checksum:i210B6021D61EC8C0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771P → S in AAQ89454. (PubMed:12975309)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti456 – 4561V → A.
Corresponds to variant rs34117815 [ dbSNP | Ensembl ].
VAR_053599

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei336 – 41176Missing in isoform 2. 1 PublicationVSP_020876Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF219137 mRNA. Translation: AAF44629.1.
AY359096 mRNA. Translation: AAQ89454.1.
BC019330 mRNA. Translation: AAH19330.1.
BC052585 mRNA. Translation: AAH52585.1.
CCDSiCCDS3044.1. [Q9NZ53-1]
RefSeqiNP_056535.1. NM_015720.3. [Q9NZ53-1]
UniGeneiHs.591290.

Genome annotation databases

EnsembliENST00000342480; ENSP00000345359; ENSG00000114631. [Q9NZ53-1]
GeneIDi50512.
KEGGihsa:50512.
UCSCiuc003ejq.3. human. [Q9NZ53-1]

Polymorphism databases

DMDMi74734719.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF219137 mRNA. Translation: AAF44629.1 .
AY359096 mRNA. Translation: AAQ89454.1 .
BC019330 mRNA. Translation: AAH19330.1 .
BC052585 mRNA. Translation: AAH52585.1 .
CCDSi CCDS3044.1. [Q9NZ53-1 ]
RefSeqi NP_056535.1. NM_015720.3. [Q9NZ53-1 ]
UniGenei Hs.591290.

3D structure databases

ProteinModelPortali Q9NZ53.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119082. 5 interactions.
IntActi Q9NZ53. 1 interaction.
STRINGi 9606.ENSP00000345359.

PTM databases

PhosphoSitei Q9NZ53.

Polymorphism databases

DMDMi 74734719.

Proteomic databases

MaxQBi Q9NZ53.
PaxDbi Q9NZ53.
PRIDEi Q9NZ53.

Protocols and materials databases

DNASUi 50512.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000342480 ; ENSP00000345359 ; ENSG00000114631 . [Q9NZ53-1 ]
GeneIDi 50512.
KEGGi hsa:50512.
UCSCi uc003ejq.3. human. [Q9NZ53-1 ]

Organism-specific databases

CTDi 50512.
GeneCardsi GC03P127348.
HGNCi HGNC:17936. PODXL2.
HPAi CAB024934.
HPA042265.
neXtProti NX_Q9NZ53.
PharmGKBi PA134860950.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG43739.
GeneTreei ENSGT00730000111323.
HOGENOMi HOG000054210.
HOVERGENi HBG082114.
InParanoidi Q9NZ53.
KOi K06818.
OMAi IPWDSTQ.
OrthoDBi EOG72C51N.
PhylomeDBi Q9NZ53.
TreeFami TF333564.

Miscellaneous databases

GenomeRNAii 50512.
NextBioi 53086.
PROi Q9NZ53.

Gene expression databases

Bgeei Q9NZ53.
CleanExi HS_PODXL2.
Genevestigatori Q9NZ53.

Family and domain databases

InterProi IPR013836. CD34/Podocalyxin.
[Graphical view ]
Pfami PF06365. CD34_antigen. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins."
    Sassetti C., Van Zante A., Rosen S.D.
    J. Biol. Chem. 275:9001-9010(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, GLYCOSYLATION.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Melanoma and Neuroblastoma.
  4. "Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x."
    Fieger C.B., Sassetti C.M., Rosen S.D.
    J. Biol. Chem. 278:27390-27398(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SELL, SUBUNIT, SULFATION AT TYR-97 AND TYR-118, GLYCOSYLATION, SIALIC ACID CONTENT, MUTAGENESIS OF TYR-97; TYR-118 AND THR-124.
  5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins."
    Kerr S.C., Fieger C.B., Snapp K.R., Rosen S.D.
    J. Immunol. 181:1480-1490(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SELL; SELE AND SELP, GLYCOSYLATION, TISSUE SPECIFICITY.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-570 AND SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
    Halim A., Ruetschi U., Larson G., Nilsson J.
    J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPDXL2_HUMAN
AccessioniPrimary (citable) accession number: Q9NZ53
Secondary accession number(s): Q6UVY4, Q8WUV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3