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Q9NZ53

- PDXL2_HUMAN

UniProt

Q9NZ53 - PDXL2_HUMAN

Protein

Podocalyxin-like protein 2

Gene

PODXL2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.1 Publication

    GO - Molecular functioni

    1. glycosaminoglycan binding Source: ProtInc
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. leukocyte tethering or rolling Source: UniProtKB

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Sialic acid

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Podocalyxin-like protein 2
    Alternative name(s):
    Endoglycan
    Gene namesi
    Name:PODXL2
    ORF Names:UNQ1861/PRO3742
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:17936. PODXL2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi97 – 971Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-118. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-118 and A-124. 1 Publication
    Mutagenesisi118 – 1181Y → F: Remains sulfated. Not sulfated and reduced rolling of Jurkat T-cells by more than 50%; when associated with F-97. The rolling of Jurkat T-cells is reduced by more than 80%; when associated with F-97 and A-124. 1 Publication
    Mutagenesisi124 – 1241T → A: Not sialylated O-linked. 1 Publication

    Organism-specific databases

    PharmGKBiPA134860950.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 605573Podocalyxin-like protein 2PRO_0000252129Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei97 – 971Sulfotyrosine1 Publication
    Modified residuei118 – 1181Sulfotyrosine1 Publication
    Glycosylationi144 – 1441O-linked (GalNAc...)1 Publication
    Glycosylationi193 – 1931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi395 – 3951N-linked (GlcNAc...)Sequence Analysis
    Modified residuei570 – 5701Phosphoserine1 Publication
    Modified residuei596 – 5961Phosphoserine3 Publications

    Post-translational modificationi

    Glycosylated; contains chondroitin sulfate. Displays sialylated O-linked oligosaccharides.1 Publication
    Sulfation is necessary for interaction with SELL. Sialylated O-linked oligosaccharides are necessary for interaction with SELL, SELE and SELP.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation

    Proteomic databases

    MaxQBiQ9NZ53.
    PaxDbiQ9NZ53.
    PRIDEiQ9NZ53.

    PTM databases

    PhosphoSiteiQ9NZ53.

    Expressioni

    Tissue specificityi

    Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.2 Publications

    Gene expression databases

    BgeeiQ9NZ53.
    CleanExiHS_PODXL2.
    GenevestigatoriQ9NZ53.

    Organism-specific databases

    HPAiCAB024934.
    HPA042265.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Interacts with SELL, SELE and SELP.2 Publications

    Protein-protein interaction databases

    BioGridi119082. 3 interactions.
    IntActiQ9NZ53. 1 interaction.
    STRINGi9606.ENSP00000345359.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NZ53.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 500468ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini522 – 60584CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei501 – 52121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni129 – 1346O-glycosylated at one site

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi162 – 19837Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the podocalyxin family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43739.
    HOGENOMiHOG000054210.
    HOVERGENiHBG082114.
    InParanoidiQ9NZ53.
    KOiK06818.
    OMAiIPWDSTQ.
    OrthoDBiEOG72C51N.
    PhylomeDBiQ9NZ53.
    TreeFamiTF333564.

    Family and domain databases

    InterProiIPR013836. CD34/Podocalyxin.
    [Graphical view]
    PfamiPF06365. CD34_antigen. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NZ53-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGRLLRAARL PPLLSPLLLL LVGGAFLGAC VAGSDEPGPE GLTSTSLLDL    50
    LLPTGLEPLD SEEPSETMGL GAGLGAPGSG FPSEENEESR ILQPPQYFWE 100
    EEEELNDSSL DLGPTADYVF PDLTEKAGSI EDTSQAQELP NLPSPLPKMN 150
    LVEPPWHMPP REEEEEEEEE EEREKEEVEK QEEEEEEELL PVNGSQEEAK 200
    PQVRDFSLTS SSQTPGATKS RHEDSGDQAS SGVEVESSMG PSLLLPSVTP 250
    TTVTPGDQDS TSQEAEATVL PAAGLGVEFE APQEASEEAT AGAAGLSGQH 300
    EEVPALPSFP QTTAPSGAEH PDEDPLGSRT SASSPLAPGD MELTPSSATL 350
    GQEDLNQQLL EGQAAEAQSR IPWDSTQVIC KDWSNLAGKN YIILNMTENI 400
    DCEVFRQHRG PQLLALVEEV LPRHGSGHHG AWHISLSKPS EKEQHLLMTL 450
    VGEQGVVPTQ DVLSMLGDIR RSLEEIGIQN YSTTSSCQAR ASQVRSDYGT 500
    LFVVLVVIGA ICIIIIALGL LYNCWQRRLP KLKHVSHGEE LRFVENGCHD 550
    NPTLDVASDS QSEMQEKHPS LNGGGALNGP GSWGALMGGK RDPEDSDVFE 600
    EDTHL 605
    Length:605
    Mass (Da):65,076
    Last modified:October 1, 2000 - v1
    Checksum:i2F9B8DC51F7FC22A
    GO
    Isoform 2 (identifier: Q9NZ53-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         336-411: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:529
    Mass (Da):56,635
    Checksum:i210B6021D61EC8C0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771P → S in AAQ89454. (PubMed:12975309)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti456 – 4561V → A.
    Corresponds to variant rs34117815 [ dbSNP | Ensembl ].
    VAR_053599

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei336 – 41176Missing in isoform 2. 1 PublicationVSP_020876Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF219137 mRNA. Translation: AAF44629.1.
    AY359096 mRNA. Translation: AAQ89454.1.
    BC019330 mRNA. Translation: AAH19330.1.
    BC052585 mRNA. Translation: AAH52585.1.
    CCDSiCCDS3044.1. [Q9NZ53-1]
    RefSeqiNP_056535.1. NM_015720.3. [Q9NZ53-1]
    UniGeneiHs.591290.

    Genome annotation databases

    EnsembliENST00000342480; ENSP00000345359; ENSG00000114631. [Q9NZ53-1]
    GeneIDi50512.
    KEGGihsa:50512.
    UCSCiuc003ejq.3. human. [Q9NZ53-1]

    Polymorphism databases

    DMDMi74734719.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF219137 mRNA. Translation: AAF44629.1 .
    AY359096 mRNA. Translation: AAQ89454.1 .
    BC019330 mRNA. Translation: AAH19330.1 .
    BC052585 mRNA. Translation: AAH52585.1 .
    CCDSi CCDS3044.1. [Q9NZ53-1 ]
    RefSeqi NP_056535.1. NM_015720.3. [Q9NZ53-1 ]
    UniGenei Hs.591290.

    3D structure databases

    ProteinModelPortali Q9NZ53.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119082. 3 interactions.
    IntActi Q9NZ53. 1 interaction.
    STRINGi 9606.ENSP00000345359.

    PTM databases

    PhosphoSitei Q9NZ53.

    Polymorphism databases

    DMDMi 74734719.

    Proteomic databases

    MaxQBi Q9NZ53.
    PaxDbi Q9NZ53.
    PRIDEi Q9NZ53.

    Protocols and materials databases

    DNASUi 50512.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000342480 ; ENSP00000345359 ; ENSG00000114631 . [Q9NZ53-1 ]
    GeneIDi 50512.
    KEGGi hsa:50512.
    UCSCi uc003ejq.3. human. [Q9NZ53-1 ]

    Organism-specific databases

    CTDi 50512.
    GeneCardsi GC03P127348.
    HGNCi HGNC:17936. PODXL2.
    HPAi CAB024934.
    HPA042265.
    neXtProti NX_Q9NZ53.
    PharmGKBi PA134860950.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43739.
    HOGENOMi HOG000054210.
    HOVERGENi HBG082114.
    InParanoidi Q9NZ53.
    KOi K06818.
    OMAi IPWDSTQ.
    OrthoDBi EOG72C51N.
    PhylomeDBi Q9NZ53.
    TreeFami TF333564.

    Miscellaneous databases

    GenomeRNAii 50512.
    NextBioi 53086.
    PROi Q9NZ53.

    Gene expression databases

    Bgeei Q9NZ53.
    CleanExi HS_PODXL2.
    Genevestigatori Q9NZ53.

    Family and domain databases

    InterProi IPR013836. CD34/Podocalyxin.
    [Graphical view ]
    Pfami PF06365. CD34_antigen. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of endoglycan, a member of the CD34/podocalyxin family of sialomucins."
      Sassetti C., Van Zante A., Rosen S.D.
      J. Biol. Chem. 275:9001-9010(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, GLYCOSYLATION.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Melanoma and Neuroblastoma.
    4. "Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x."
      Fieger C.B., Sassetti C.M., Rosen S.D.
      J. Biol. Chem. 278:27390-27398(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SELL, SUBUNIT, SULFATION AT TYR-97 AND TYR-118, GLYCOSYLATION, SIALIC ACID CONTENT, MUTAGENESIS OF TYR-97; TYR-118 AND THR-124.
    5. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins."
      Kerr S.C., Fieger C.B., Snapp K.R., Rosen S.D.
      J. Immunol. 181:1480-1490(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SELL; SELE AND SELP, GLYCOSYLATION, TISSUE SPECIFICITY.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-570 AND SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A., Ruetschi U., Larson G., Nilsson J.
      J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-144, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPDXL2_HUMAN
    AccessioniPrimary (citable) accession number: Q9NZ53
    Secondary accession number(s): Q6UVY4, Q8WUV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3