Q9NZ43 (USE1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle transport protein USE1

Alternative name(s):
Putative MAPK-activating protein PM26
USE1-like protein
p31

Gene names

Name:	USE1
Synonyms:	USE1L
ORF Names:	MDS032

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Ref.7
Subunit structure	Component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. Interacts directly with STX18. Ref.6 Ref.7
Subcellular location	Endoplasmic reticulum membrane; Single-pass type IV membrane protein Ref.6.
Sequence similarities	Belongs to the USE1 family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	endoplasmic reticulum tubular network organization Inferred from electronic annotation. Source: Compara lysosomal transport Inferred from sequence or structural similarity PubMed 16354670. Source: HGNC protein catabolic process Inferred from sequence or structural similarity PubMed 16354670. Source: HGNC protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of ER to Golgi vesicle-mediated transport Inferred from electronic annotation. Source: Compara secretion by cell Inferred from sequence or structural similarity PubMed 16354670. Source: HGNC vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.6. Source: HGNC endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NZ43-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NZ43-2)

The sequence of this isoform differs from the canonical sequence as follows:
     142-146: GVAGS → PCHTH
     147-259: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NZ43-3)

The sequence of this isoform differs from the canonical sequence as follows:
     132-135: EPEM → GESP
     136-259: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Vesicle transport protein USE1		PRO_0000215579
Regions
	Topological domain	1 – 231	231	Cytoplasmic Potential
	Transmembrane	232 – 252	21	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	253 – 259	7	Lumenal Potential
	Coiled coil	152 – 231	80	Potential
Natural variations
	Alternative sequence	132 – 135	4	EPEM → GESP in isoform 3.		VSP_012662
	Alternative sequence	136 – 259	124	Missing in isoform 3.		VSP_012663
	Alternative sequence	142 – 146	5	GVAGS → PCHTH in isoform 2.		VSP_012664
	Alternative sequence	147 – 259	113	Missing in isoform 2.		VSP_012665
	Natural variant	154	1	L → S. Ref.1 Ref.3 Ref.5 Corresponds to variant rs414528 [ dbSNP | Ensembl ].		VAR_021052
Experimental info
	Sequence conflict	38	1	A → V in AAH06005. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 18, 2010. Version 2. Checksum: 6538ED0B231AEC05	FASTA	259	29,371
	10 20 30 40 50 60 MAASRLELNL VRLLSRCEAM AAEKRDPDEW RLEKYVGALE DMLQALKVHA SKPASEVINE 70 80 90 100 110 120 YSWKVDFLKG MLQAEKLTSS SEKALANQFL APGRVPTTAR ERVPATKTVH LQSRARYTSE 130 140 150 160 170 180 MRSELLGTDS AEPEMDVRKR TGVAGSQPVS EKQLAAELDL VLQRHQNLQE KLAEEMLGLA 190 200 210 220 230 240 RSLKTNTLAA QSVIKKDNQT LSHSLKMADQ NLEKLKTESE RLEQHTQKSV NWLLWAMLII 250 VCFIFISMIL FIRIMPKLK
	Isoform 2 [UniParc]. Checksum: 307946785B1BAC38	FASTA	146	16,511
	10 20 30 40 50 60 MAASRLELNL VRLLSRCEAM AAEKRDPDEW RLEKYVGALE DMLQALKVHA SKPASEVINE 70 80 90 100 110 120 YSWKVDFLKG MLQAEKLTSS SEKALANQFL APGRVPTTAR ERVPATKTVH LQSRARYTSE 130 140 MRSELLGTDS AEPEMDVRKR TPCHTH
	Isoform 3 [UniParc]. Checksum: 22DF3FEF395B8489	FASTA	135	15,063
	10 20 30 40 50 60 MAASRLELNL VRLLSRCEAM AAEKRDPDEW RLEKYVGALE DMLQALKVHA SKPASEVINE 70 80 90 100 110 120 YSWKVDFLKG MLQAEKLTSS SEKALANQFL APGRVPTTAR ERVPATKTVH LQSRARYTSE 130 MRSELLGTDS AGESP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Large-scale identification and characterization of human genes that activate NF-kappaB and MAPK signaling pathways." Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y., Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S. Oncogene 22:3307-3318(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-154. Tissue: Lung fibroblast.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Mammary gland.
[3]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients." Zhao M., Gu J., Li N., Peng Y., Han Z., Chen Z. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-154. Tissue: Hematopoietic stem cell.
[4]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT SER-154. Tissue: Brain and Skeletal muscle.
[6]	"Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi." Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M., Tani K., Yamamoto A., Tohyama M., Tagaya M. EMBO J. 23:1267-1278(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH STX18 AND SEC22B, INTERACTION WITH STX18.
[7]	"Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion." Nakajima K., Hirose H., Taniguchi M., Kurashina H., Arasaki K., Nagahama M., Tani K., Yamamoto A., Tagaya M. EMBO J. 23:3216-3226(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH STX18; BNIP1 AND SEC22B.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB097050 mRNA. Translation: BAC77403.1. AK074683 mRNA. Translation: BAC11136.1. AF220052 mRNA. Translation: AAF67645.1. AC020913 Genomic DNA. No translation available. BC006005 mRNA. Translation: AAH06005.1. BC008455 mRNA. Translation: AAH08455.1.
IPI	IPI00020515. IPI00549501. IPI00549814.
RefSeq	NP_060937.1. NM_018467.3.
UniGene	Hs.16187.
3D structure databases
ProteinModelPortal	Q9NZ43.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NZ43. 1 interaction.
MINT	MINT-1463844.
STRING	9606.ENSP00000263897.
PTM databases
PhosphoSite	Q9NZ43.
Polymorphism databases
DMDM	296452858.
Proteomic databases
PaxDb	Q9NZ43.
PRIDE	Q9NZ43.
Protocols and materials databases
DNASU	55850.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263897; ENSP00000263897; ENSG00000053501. ENST00000595101; ENSP00000472362; ENSG00000053501. ENST00000596136; ENSP00000473239; ENSG00000053501.
GeneID	55850.
KEGG	hsa:55850.
UCSC	uc002nfn.2. human. uc002nfo.2. human. uc010eal.1. human.
Organism-specific databases
CTD	55850.
GeneCards	GC19P017326.
HGNC	HGNC:30882. USE1.
HPA	HPA026851.
MIM	610675. gene.
neXtProt	NX_Q9NZ43.
PharmGKB	PA162408692.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG299387.
HOGENOM	HOG000007677.
HOVERGEN	HBG079328.
InParanoid	Q9NZ43.
KO	K08507.
OMA	SLRMADQ.
OrthoDB	EOG402WSW.
Gene expression databases
ArrayExpress	Q9NZ43.
Bgee	Q9NZ43.
CleanEx	HS_USE1.
Genevestigator	Q9NZ43.
GermOnline	ENSG00000053501. Homo sapiens.
Family and domain databases
InterPro	IPR019150. Vesicle_transport_protein_Use1. [Graphical view]
Pfam	PF09753. Use1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55850.
NextBio	61111.
PMAP-CutDB	Q9NZ43.
SOURCE	Search...

Entry information

Entry name

USE1_HUMAN

Accession

Primary (citable) accession number: Q9NZ43
Secondary accession number(s): Q8NCK1, Q9BRT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.