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Q9NYZ4

- SIGL8_HUMAN

UniProt

Q9NYZ4 - SIGL8_HUMAN

Protein

Sialic acid-binding Ig-like lectin 8

Gene

SIGLEC8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (08 Nov 2002)
      Previous versions | rss
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    Functioni

    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251Sialic acidBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: ProtInc
    2. protein binding Source: IntAct
    3. transmembrane signaling receptor activity Source: ProtInc

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. signal transduction Source: ProtInc

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Lectin

    Protein family/group databases

    MEROPSiI43.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialic acid-binding Ig-like lectin 8
    Short name:
    Siglec-8
    Alternative name(s):
    Sialoadhesin family member 2
    Short name:
    SAF-2
    Gene namesi
    Name:SIGLEC8
    Synonyms:SAF2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:10877. SIGLEC8.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: ProtInc

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35778.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Chaini17 – 499483Sialic acid-binding Ig-like lectin 8PRO_0000014948Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi42 ↔ 181PROSITE-ProRule annotation
    Disulfide bondi47 ↔ 107PROSITE-ProRule annotation
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi175 ↔ 224PROSITE-ProRule annotation
    Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi283 ↔ 328PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9NYZ4.
    PRIDEiQ9NYZ4.

    PTM databases

    PhosphoSiteiQ9NYZ4.

    Expressioni

    Tissue specificityi

    Expressed specifically on eosinophils.

    Gene expression databases

    ArrayExpressiQ9NYZ4.
    BgeeiQ9NYZ4.
    CleanExiHS_SIGLEC8.
    GenevestigatoriQ9NYZ4.

    Organism-specific databases

    HPAiHPA012556.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TFF1P041553EBI-4314991,EBI-743871

    Protein-protein interaction databases

    BioGridi118057. 1 interaction.
    IntActiQ9NYZ4. 1 interaction.
    STRINGi9606.ENSP00000321077.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NYZ4.
    SMRiQ9NYZ4. Positions 27-244.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini17 – 363347ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini385 – 499115CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei364 – 38421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 12384Ig-like V-typeAdd
    BLAST
    Domaini157 – 24084Ig-like C2-type 1Add
    BLAST
    Domaini246 – 34499Ig-like C2-type 2Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi445 – 4506ITIM motif
    Motifi468 – 4736SLAM-like motif

    Domaini

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG147919.
    HOGENOMiHOG000236324.
    HOVERGENiHBG036161.
    InParanoidiQ9NYZ4.
    KOiK06748.
    OMAiLERGSMK.
    PhylomeDBiQ9NYZ4.
    TreeFamiTF332441.

    Family and domain databases

    Gene3Di2.60.40.10. 3 hits.
    InterProiIPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR013106. Ig_V-set.
    IPR013151. Immunoglobulin.
    [Graphical view]
    PfamiPF00047. ig. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    SM00408. IGc2. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 2 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NYZ4-1) [UniParc]FASTAAdd to Basket

    Also known as: Long

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLLLLLLLPL LWGTKGMEGD RQYGDGYLLQ VQELVTVQEG LCVHVPCSFS    50
    YPQDGWTDSD PVHGYWFRAG DRPYQDAPVA TNNPDREVQA ETQGRFQLLG 100
    DIWSNDCSLS IRDARKRDKG SYFFRLERGS MKWSYKSQLN YKTKQLSVFV 150
    TALTHRPDIL ILGTLESGHS RNLTCSVPWA CKQGTPPMIS WIGASVSSPG 200
    PTTARSSVLT LTPKPQDHGT SLTCQVTLPG TGVTTTSTVR LDVSYPPWNL 250
    TMTVFQGDAT ASTALGNGSS LSVLEGQSLR LVCAVNSNPP ARLSWTRGSL 300
    TLCPSRSSNP GLLELPRVHV RDEGEFTCRA QNAQGSQHIS LSLSLQNEGT 350
    GTSRPVSQVT LAAVGGAGAT ALAFLSFCII FIIVRSCRKK SARPAAGVGD 400
    TGMEDAKAIR GSASQGPLTE SWKDGNPLKK PPPAVAPSSG EEGELHYATL 450
    SFHKVKPQDP QGQEATDSEY SEIKIHKRET AETQACLRNH NPSSKEVRG 499
    Length:499
    Mass (Da):54,042
    Last modified:November 8, 2002 - v2
    Checksum:i086EFF989B74123C
    GO
    Isoform 2 (identifier: Q9NYZ4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         152-245: ALTHRPDILI...TSTVRLDVSY → D

    Show »
    Length:406
    Mass (Da):44,360
    Checksum:iB23D94FA1DB4741A
    GO
    Isoform 3 (identifier: Q9NYZ4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         416-499: GPLTESWKDG...HNPSSKEVRG → VSDVGFSTPSIQPGHL

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:431
    Mass (Da):46,434
    Checksum:i49B91B84CE67B118
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti170 – 1701S → P.1 Publication
    Corresponds to variant rs10409962 [ dbSNP | Ensembl ].
    VAR_021487
    Natural varianti282 – 2821V → L Found in a renal cell carcinoma sample; somatic mutation. 1 Publication
    VAR_064751
    Natural varianti388 – 3881R → G.
    Corresponds to variant rs3829659 [ dbSNP | Ensembl ].
    VAR_049930

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei152 – 24594ALTHR…LDVSY → D in isoform 2. 1 PublicationVSP_002559Add
    BLAST
    Alternative sequencei416 – 49984GPLTE…KEVRG → VSDVGFSTPSIQPGHL in isoform 3. 2 PublicationsVSP_002560Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF287892 Genomic DNA. Translation: AAG00573.1.
    AF223403 mRNA. Translation: AAF34702.1.
    AF195092 mRNA. Translation: AAF27622.1.
    AF310234 mRNA. Translation: AAK55140.1.
    BC053319 mRNA. Translation: AAH53319.1.
    CCDSiCCDS33086.1. [Q9NYZ4-1]
    RefSeqiNP_055257.2. NM_014442.2. [Q9NYZ4-1]
    UniGeneiHs.447899.

    Genome annotation databases

    EnsembliENST00000321424; ENSP00000321077; ENSG00000105366. [Q9NYZ4-1]
    ENST00000340550; ENSP00000339448; ENSG00000105366. [Q9NYZ4-2]
    GeneIDi27181.
    KEGGihsa:27181.
    UCSCiuc002pwt.3. human. [Q9NYZ4-1]
    uc010eox.2. human. [Q9NYZ4-2]

    Polymorphism databases

    DMDMi25009268.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - Glycan Binding

    Siglec-8

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF287892 Genomic DNA. Translation: AAG00573.1 .
    AF223403 mRNA. Translation: AAF34702.1 .
    AF195092 mRNA. Translation: AAF27622.1 .
    AF310234 mRNA. Translation: AAK55140.1 .
    BC053319 mRNA. Translation: AAH53319.1 .
    CCDSi CCDS33086.1. [Q9NYZ4-1 ]
    RefSeqi NP_055257.2. NM_014442.2. [Q9NYZ4-1 ]
    UniGenei Hs.447899.

    3D structure databases

    ProteinModelPortali Q9NYZ4.
    SMRi Q9NYZ4. Positions 27-244.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118057. 1 interaction.
    IntActi Q9NYZ4. 1 interaction.
    STRINGi 9606.ENSP00000321077.

    Protein family/group databases

    MEROPSi I43.001.

    PTM databases

    PhosphoSitei Q9NYZ4.

    Polymorphism databases

    DMDMi 25009268.

    Proteomic databases

    PaxDbi Q9NYZ4.
    PRIDEi Q9NYZ4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000321424 ; ENSP00000321077 ; ENSG00000105366 . [Q9NYZ4-1 ]
    ENST00000340550 ; ENSP00000339448 ; ENSG00000105366 . [Q9NYZ4-2 ]
    GeneIDi 27181.
    KEGGi hsa:27181.
    UCSCi uc002pwt.3. human. [Q9NYZ4-1 ]
    uc010eox.2. human. [Q9NYZ4-2 ]

    Organism-specific databases

    CTDi 27181.
    GeneCardsi GC19M051956.
    H-InvDB HIX0027458.
    HGNCi HGNC:10877. SIGLEC8.
    HPAi HPA012556.
    MIMi 605639. gene.
    neXtProti NX_Q9NYZ4.
    PharmGKBi PA35778.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG147919.
    HOGENOMi HOG000236324.
    HOVERGENi HBG036161.
    InParanoidi Q9NYZ4.
    KOi K06748.
    OMAi LERGSMK.
    PhylomeDBi Q9NYZ4.
    TreeFami TF332441.

    Miscellaneous databases

    GeneWikii SIGLEC8.
    GenomeRNAii 27181.
    NextBioi 50012.
    PROi Q9NYZ4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NYZ4.
    Bgeei Q9NYZ4.
    CleanExi HS_SIGLEC8.
    Genevestigatori Q9NYZ4.

    Family and domain databases

    Gene3Di 2.60.40.10. 3 hits.
    InterProi IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR003598. Ig_sub2.
    IPR013106. Ig_V-set.
    IPR013151. Immunoglobulin.
    [Graphical view ]
    Pfami PF00047. ig. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    SM00408. IGc2. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a siglec8 variant containing cytoplasmic tyrosine-based motifs, and mapping of the siglec8 gene."
      Foussias G., Yousef G.M., Diamandis E.P.
      Biochem. Biophys. Res. Commun. 278:775-781(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    3. "Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily."
      Floyd H., Ni J., Cornish A.L., Zeng Z., Liu D., Carter K.C., Steel J., Crocker P.R.
      J. Biol. Chem. 275:861-866(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
      Tissue: Eosinophil.
    4. Floyd H., Zhang J.Q., Crocker P.R.
      Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-170.
      Tissue: Spleen.
    6. Cited for: VARIANT LEU-282.

    Entry informationi

    Entry nameiSIGL8_HUMAN
    AccessioniPrimary (citable) accession number: Q9NYZ4
    Secondary accession number(s): Q7Z728
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2002
    Last sequence update: November 8, 2002
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3