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Q9NYZ4

- SIGL8_HUMAN

UniProt

Q9NYZ4 - SIGL8_HUMAN

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Protein

Sialic acid-binding Ig-like lectin 8

Gene
SIGLEC8, SAF2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251Sialic acid By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: ProtInc
  2. protein binding Source: IntAct
  3. transmembrane signaling receptor activity Source: ProtInc

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Protein family/group databases

MEROPSiI43.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid-binding Ig-like lectin 8
Short name:
Siglec-8
Alternative name(s):
Sialoadhesin family member 2
Short name:
SAF-2
Gene namesi
Name:SIGLEC8
Synonyms:SAF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:10877. SIGLEC8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 363347Extracellular Reviewed predictionAdd
BLAST
Transmembranei364 – 38421Helical; Reviewed predictionAdd
BLAST
Topological domaini385 – 499115Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35778.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616 Reviewed predictionAdd
BLAST
Chaini17 – 499483Sialic acid-binding Ig-like lectin 8PRO_0000014948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 181 By similarity
Disulfide bondi47 ↔ 107 By similarity
Glycosylationi172 – 1721N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi175 ↔ 224 By similarity
Glycosylationi249 – 2491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi267 – 2671N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi283 ↔ 328 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NYZ4.
PRIDEiQ9NYZ4.

PTM databases

PhosphoSiteiQ9NYZ4.

Expressioni

Tissue specificityi

Expressed specifically on eosinophils.

Gene expression databases

ArrayExpressiQ9NYZ4.
BgeeiQ9NYZ4.
CleanExiHS_SIGLEC8.
GenevestigatoriQ9NYZ4.

Organism-specific databases

HPAiHPA012556.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TFF1P041553EBI-4314991,EBI-743871

Protein-protein interaction databases

BioGridi118057. 1 interaction.
IntActiQ9NYZ4. 1 interaction.
STRINGi9606.ENSP00000321077.

Structurei

3D structure databases

ProteinModelPortaliQ9NYZ4.
SMRiQ9NYZ4. Positions 27-244.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 12384Ig-like V-typeAdd
BLAST
Domaini157 – 24084Ig-like C2-type 1Add
BLAST
Domaini246 – 34499Ig-like C2-type 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi445 – 4506ITIM motif
Motifi468 – 4736SLAM-like motif

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG147919.
HOGENOMiHOG000236324.
HOVERGENiHBG036161.
InParanoidiQ9NYZ4.
KOiK06748.
OMAiLERGSMK.
PhylomeDBiQ9NYZ4.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view]
PfamiPF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NYZ4-1) [UniParc]FASTAAdd to Basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLLLLLLLPL LWGTKGMEGD RQYGDGYLLQ VQELVTVQEG LCVHVPCSFS    50
YPQDGWTDSD PVHGYWFRAG DRPYQDAPVA TNNPDREVQA ETQGRFQLLG 100
DIWSNDCSLS IRDARKRDKG SYFFRLERGS MKWSYKSQLN YKTKQLSVFV 150
TALTHRPDIL ILGTLESGHS RNLTCSVPWA CKQGTPPMIS WIGASVSSPG 200
PTTARSSVLT LTPKPQDHGT SLTCQVTLPG TGVTTTSTVR LDVSYPPWNL 250
TMTVFQGDAT ASTALGNGSS LSVLEGQSLR LVCAVNSNPP ARLSWTRGSL 300
TLCPSRSSNP GLLELPRVHV RDEGEFTCRA QNAQGSQHIS LSLSLQNEGT 350
GTSRPVSQVT LAAVGGAGAT ALAFLSFCII FIIVRSCRKK SARPAAGVGD 400
TGMEDAKAIR GSASQGPLTE SWKDGNPLKK PPPAVAPSSG EEGELHYATL 450
SFHKVKPQDP QGQEATDSEY SEIKIHKRET AETQACLRNH NPSSKEVRG 499
Length:499
Mass (Da):54,042
Last modified:November 8, 2002 - v2
Checksum:i086EFF989B74123C
GO
Isoform 2 (identifier: Q9NYZ4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     152-245: ALTHRPDILI...TSTVRLDVSY → D

Show »
Length:406
Mass (Da):44,360
Checksum:iB23D94FA1DB4741A
GO
Isoform 3 (identifier: Q9NYZ4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-499: GPLTESWKDG...HNPSSKEVRG → VSDVGFSTPSIQPGHL

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:431
Mass (Da):46,434
Checksum:i49B91B84CE67B118
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti170 – 1701S → P.1 Publication
Corresponds to variant rs10409962 [ dbSNP | Ensembl ].
VAR_021487
Natural varianti282 – 2821V → L Found in a renal cell carcinoma sample; somatic mutation. 1 Publication
VAR_064751
Natural varianti388 – 3881R → G.
Corresponds to variant rs3829659 [ dbSNP | Ensembl ].
VAR_049930

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei152 – 24594ALTHR…LDVSY → D in isoform 2. VSP_002559Add
BLAST
Alternative sequencei416 – 49984GPLTE…KEVRG → VSDVGFSTPSIQPGHL in isoform 3. VSP_002560Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF287892 Genomic DNA. Translation: AAG00573.1.
AF223403 mRNA. Translation: AAF34702.1.
AF195092 mRNA. Translation: AAF27622.1.
AF310234 mRNA. Translation: AAK55140.1.
BC053319 mRNA. Translation: AAH53319.1.
CCDSiCCDS33086.1. [Q9NYZ4-1]
RefSeqiNP_055257.2. NM_014442.2. [Q9NYZ4-1]
UniGeneiHs.447899.

Genome annotation databases

EnsembliENST00000321424; ENSP00000321077; ENSG00000105366. [Q9NYZ4-1]
ENST00000340550; ENSP00000339448; ENSG00000105366. [Q9NYZ4-2]
GeneIDi27181.
KEGGihsa:27181.
UCSCiuc002pwt.3. human. [Q9NYZ4-1]
uc010eox.2. human. [Q9NYZ4-2]

Polymorphism databases

DMDMi25009268.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

Siglec-8

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF287892 Genomic DNA. Translation: AAG00573.1 .
AF223403 mRNA. Translation: AAF34702.1 .
AF195092 mRNA. Translation: AAF27622.1 .
AF310234 mRNA. Translation: AAK55140.1 .
BC053319 mRNA. Translation: AAH53319.1 .
CCDSi CCDS33086.1. [Q9NYZ4-1 ]
RefSeqi NP_055257.2. NM_014442.2. [Q9NYZ4-1 ]
UniGenei Hs.447899.

3D structure databases

ProteinModelPortali Q9NYZ4.
SMRi Q9NYZ4. Positions 27-244.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 118057. 1 interaction.
IntActi Q9NYZ4. 1 interaction.
STRINGi 9606.ENSP00000321077.

Protein family/group databases

MEROPSi I43.001.

PTM databases

PhosphoSitei Q9NYZ4.

Polymorphism databases

DMDMi 25009268.

Proteomic databases

PaxDbi Q9NYZ4.
PRIDEi Q9NYZ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000321424 ; ENSP00000321077 ; ENSG00000105366 . [Q9NYZ4-1 ]
ENST00000340550 ; ENSP00000339448 ; ENSG00000105366 . [Q9NYZ4-2 ]
GeneIDi 27181.
KEGGi hsa:27181.
UCSCi uc002pwt.3. human. [Q9NYZ4-1 ]
uc010eox.2. human. [Q9NYZ4-2 ]

Organism-specific databases

CTDi 27181.
GeneCardsi GC19M051956.
H-InvDB HIX0027458.
HGNCi HGNC:10877. SIGLEC8.
HPAi HPA012556.
MIMi 605639. gene.
neXtProti NX_Q9NYZ4.
PharmGKBi PA35778.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG147919.
HOGENOMi HOG000236324.
HOVERGENi HBG036161.
InParanoidi Q9NYZ4.
KOi K06748.
OMAi LERGSMK.
PhylomeDBi Q9NYZ4.
TreeFami TF332441.

Miscellaneous databases

GeneWikii SIGLEC8.
GenomeRNAii 27181.
NextBioi 50012.
PROi Q9NYZ4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9NYZ4.
Bgeei Q9NYZ4.
CleanExi HS_SIGLEC8.
Genevestigatori Q9NYZ4.

Family and domain databases

Gene3Di 2.60.40.10. 3 hits.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR013151. Immunoglobulin.
[Graphical view ]
Pfami PF00047. ig. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of a siglec8 variant containing cytoplasmic tyrosine-based motifs, and mapping of the siglec8 gene."
    Foussias G., Yousef G.M., Diamandis E.P.
    Biochem. Biophys. Res. Commun. 278:775-781(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
  3. "Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily."
    Floyd H., Ni J., Cornish A.L., Zeng Z., Liu D., Carter K.C., Steel J., Crocker P.R.
    J. Biol. Chem. 275:861-866(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Tissue: Eosinophil.
  4. Floyd H., Zhang J.Q., Crocker P.R.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT PRO-170.
    Tissue: Spleen.
  6. Cited for: VARIANT LEU-282.

Entry informationi

Entry nameiSIGL8_HUMAN
AccessioniPrimary (citable) accession number: Q9NYZ4
Secondary accession number(s): Q7Z728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: July 9, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi