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Q9NYX4

- CALY_HUMAN

UniProt

Q9NYX4 - CALY_HUMAN

Protein

Neuron-specific vesicular protein calcyon

Gene

CALY

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis.1 Publication

    GO - Molecular functioni

    1. clathrin light chain binding Source: UniProtKB

    GO - Biological processi

    1. clathrin coat assembly Source: UniProtKB
    2. dopamine receptor signaling pathway Source: InterPro
    3. endocytosis Source: UniProtKB-KW
    4. positive regulation of endocytosis Source: UniProtKB

    Keywords - Biological processi

    Endocytosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuron-specific vesicular protein calcyon
    Gene namesi
    Name:CALY
    Synonyms:DRD1IP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 10

    Organism-specific databases

    HGNCiHGNC:17938. CALY.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: UniProtKB
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162380987.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 217217Neuron-specific vesicular protein calcyonPRO_0000164368Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi73 – 731N-linked (GlcNAc...)Curated

    Post-translational modificationi

    Glycosylated.2 Publications

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9NYX4.
    PRIDEiQ9NYX4.

    PTM databases

    PhosphoSiteiQ9NYX4.

    Expressioni

    Tissue specificityi

    Expressed in the pyramidal cells of the prefrontal cortex, in hippothalamus and in caudate nucleus. No expression in spleen. Up-regulated in the prefrontal cortex of schizophrenic patients with nearly twice the levels of non-schizophrenics.1 Publication

    Gene expression databases

    BgeeiQ9NYX4.
    CleanExiHS_CALY.
    GenevestigatoriQ9NYX4.

    Organism-specific databases

    HPAiHPA042283.

    Interactioni

    Subunit structurei

    Interacts with CLTA.1 Publication

    Protein-protein interaction databases

    MINTiMINT-123006.
    STRINGi9606.ENSP00000252939.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NYX4.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8787ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini109 – 217109CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei88 – 10821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi182 – 1887Poly-Ala

    Sequence similaritiesi

    Belongs to the NSG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43575.
    HOGENOMiHOG000030909.
    HOVERGENiHBG012403.
    InParanoidiQ9NYX4.
    KOiK15493.
    OMAiDQFSCPD.
    OrthoDBiEOG71CFNC.
    PhylomeDBiQ9NYX4.
    TreeFamiTF332232.

    Family and domain databases

    InterProiIPR009431. Calcyon_neuron-sp.
    [Graphical view]
    PfamiPF06387. Calcyon. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002383. Calcyon. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NYX4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVKLGCSFSG KPGKDPGDQD GAAMDSVPLI SPLDISQLQP PLPDQVVIKT    50
    QTEYQLSSPD QQNFPDLEGQ RLNCSHPEEG RRLPTARMIA FAMALLGCVL 100
    IMYKAIWYDQ FTCPDGFLLR HKICTPLTLE MYYTEMDPER HRSILAAIGA 150
    YPLSRKHGTE TPAAWGDGYR AAKEERKGPT QAGAAAAATE PPGKPSAKAE 200
    KEAARKAAGS AAPPPAQ 217
    Length:217
    Mass (Da):23,434
    Last modified:October 1, 2000 - v1
    Checksum:i7B3ACA9042819F58
    GO
    Isoform 2 (identifier: Q9NYX4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         121-124: HKIC → GPEF
         125-217: Missing.

    Note: Gene prediction based on EST data.

    Show »
    Length:124
    Mass (Da):13,701
    Checksum:i5B3D77A683A73CC9
    GO
    Isoform 3 (identifier: Q9NYX4-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         83-217: LPTARMIAFA...AGSAAPPPAQ → VTTPTPPTVG...RCRLTKQWRS

    Note: Gene prediction based on EST data.

    Show »
    Length:158
    Mass (Da):17,547
    Checksum:i3451BF975275F836
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei83 – 217135LPTAR…PPPAQ → VTTPTPPTVGTQDRRRRWKA AGGAWVPSRPVWGPLALREE RVAVSHPAQSWPHRLPCTHQ APSRGCRCRLTKQWRS in isoform 3. CuratedVSP_034473Add
    BLAST
    Alternative sequencei121 – 1244HKIC → GPEF in isoform 2. CuratedVSP_034474
    Alternative sequencei125 – 21793Missing in isoform 2. CuratedVSP_034475Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF225903 mRNA. Translation: AAF34714.1.
    AL360181 Genomic DNA. Translation: CAH70279.1.
    AL360181 Genomic DNA. Translation: CAH70280.1.
    AL360181 Genomic DNA. Translation: CAH70281.1.
    CH471211 Genomic DNA. Translation: EAW61334.1.
    BC038978 mRNA. Translation: AAH38978.1.
    CCDSiCCDS7678.1. [Q9NYX4-1]
    RefSeqiNP_056537.1. NM_015722.3. [Q9NYX4-1]
    XP_005252742.1. XM_005252685.2. [Q9NYX4-1]
    UniGeneiHs.148680.

    Genome annotation databases

    EnsembliENST00000252939; ENSP00000252939; ENSG00000130643. [Q9NYX4-1]
    ENST00000368555; ENSP00000357543; ENSG00000130643. [Q9NYX4-3]
    ENST00000368558; ENSP00000357546; ENSG00000130643. [Q9NYX4-2]
    GeneIDi50632.
    KEGGihsa:50632.
    UCSCiuc001lmo.2. human. [Q9NYX4-1]

    Polymorphism databases

    DMDMi17374622.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF225903 mRNA. Translation: AAF34714.1 .
    AL360181 Genomic DNA. Translation: CAH70279.1 .
    AL360181 Genomic DNA. Translation: CAH70280.1 .
    AL360181 Genomic DNA. Translation: CAH70281.1 .
    CH471211 Genomic DNA. Translation: EAW61334.1 .
    BC038978 mRNA. Translation: AAH38978.1 .
    CCDSi CCDS7678.1. [Q9NYX4-1 ]
    RefSeqi NP_056537.1. NM_015722.3. [Q9NYX4-1 ]
    XP_005252742.1. XM_005252685.2. [Q9NYX4-1 ]
    UniGenei Hs.148680.

    3D structure databases

    ProteinModelPortali Q9NYX4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-123006.
    STRINGi 9606.ENSP00000252939.

    Chemistry

    DrugBanki DB00714. Apomorphine.
    DB00363. Clozapine.
    DB00875. Flupenthixol.
    DB00831. Trifluoperazine.

    PTM databases

    PhosphoSitei Q9NYX4.

    Polymorphism databases

    DMDMi 17374622.

    Proteomic databases

    PaxDbi Q9NYX4.
    PRIDEi Q9NYX4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000252939 ; ENSP00000252939 ; ENSG00000130643 . [Q9NYX4-1 ]
    ENST00000368555 ; ENSP00000357543 ; ENSG00000130643 . [Q9NYX4-3 ]
    ENST00000368558 ; ENSP00000357546 ; ENSG00000130643 . [Q9NYX4-2 ]
    GeneIDi 50632.
    KEGGi hsa:50632.
    UCSCi uc001lmo.2. human. [Q9NYX4-1 ]

    Organism-specific databases

    CTDi 50632.
    GeneCardsi GC10M135138.
    HGNCi HGNC:17938. CALY.
    HPAi HPA042283.
    MIMi 604647. gene.
    neXtProti NX_Q9NYX4.
    PharmGKBi PA162380987.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43575.
    HOGENOMi HOG000030909.
    HOVERGENi HBG012403.
    InParanoidi Q9NYX4.
    KOi K15493.
    OMAi DQFSCPD.
    OrthoDBi EOG71CFNC.
    PhylomeDBi Q9NYX4.
    TreeFami TF332232.

    Miscellaneous databases

    GeneWikii DRD1IP.
    GenomeRNAii 50632.
    NextBioi 53168.
    PROi Q9NYX4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9NYX4.
    CleanExi HS_CALY.
    Genevestigatori Q9NYX4.

    Family and domain databases

    InterProi IPR009431. Calcyon_neuron-sp.
    [Graphical view ]
    Pfami PF06387. Calcyon. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002383. Calcyon. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Dual signaling regulated by calcyon, a D1 dopamine receptor interacting protein."
      Lezcano N., Mrzljak L., Eubanks S., Levenson R., Goldman-Rakic P., Bergson C.
      Science 287:1660-1664(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: RETRACTION.
    3. "The DNA sequence and comparative analysis of human chromosome 10."
      Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
      , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
      Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Up-regulation of the D1 dopamine receptor-interacting protein, calcyon, in patients with schizophrenia."
      Koh P.O., Bergson C., Undie A.S., Goldman-Rakic P.S., Lidow M.S.
      Arch. Gen. Psychiatry 60:311-319(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Elevated intracellular calcium triggers recruitment of the receptor cross-talk accessory protein calcyon to the plasma membrane."
      Ali M.K., Bergson C.
      J. Biol. Chem. 278:51654-51663(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis."
      Xiao J., Dai R., Negyessy L., Bergson C.
      J. Biol. Chem. 281:15182-15193(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CLTA.
    9. "Enrichment of glycopeptides for glycan structure and attachment site identification."
      Nilsson J., Rueetschi U., Halim A., Hesse C., Carlsohn E., Brinkmalm G., Larson G.
      Nat. Methods 6:809-811(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
      Halim A., Ruetschi U., Larson G., Nilsson J.
      J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiCALY_HUMAN
    AccessioniPrimary (citable) accession number: Q9NYX4
    Secondary accession number(s): Q5VWX3, Q5VWY5, Q5VWY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 10
      Human chromosome 10: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3