Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuron-specific vesicular protein calcyon

Gene

CALY

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis.1 Publication

GO - Molecular functioni

  • clathrin light chain binding Source: UniProtKB

GO - Biological processi

  • clathrin coat assembly Source: UniProtKB
  • dopamine receptor signaling pathway Source: InterPro
  • endocytosis Source: UniProtKB-KW
  • positive regulation of endocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130643-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuron-specific vesicular protein calcyon
Gene namesi
Name:CALY
Synonyms:DRD1IP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:17938. CALY.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 87ExtracellularSequence analysisAdd BLAST87
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 217CytoplasmicSequence analysisAdd BLAST109

GO - Cellular componenti

  • cytoplasmic vesicle Source: UniProtKB
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi50632.
OpenTargetsiENSG00000130643.
PharmGKBiPA162380987.

Chemistry databases

DrugBankiDB00714. Apomorphine.
DB00363. Clozapine.
DB00831. Trifluoperazine.

Polymorphism and mutation databases

BioMutaiCALY.
DMDMi17374622.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001643681 – 217Neuron-specific vesicular protein calcyonAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi73N-linked (GlcNAc...)Curated1

Post-translational modificationi

Glycosylated.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9NYX4.
PeptideAtlasiQ9NYX4.
PRIDEiQ9NYX4.

PTM databases

iPTMnetiQ9NYX4.
PhosphoSitePlusiQ9NYX4.

Expressioni

Tissue specificityi

Expressed in the pyramidal cells of the prefrontal cortex, in hippothalamus and in caudate nucleus. No expression in spleen. Up-regulated in the prefrontal cortex of schizophrenic patients with nearly twice the levels of non-schizophrenics.1 Publication

Gene expression databases

BgeeiENSG00000130643.
CleanExiHS_CALY.
GenevisibleiQ9NYX4. HS.

Organism-specific databases

HPAiHPA042283.

Interactioni

Subunit structurei

Interacts with CLTA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CLTAP094964EBI-10904725,EBI-1171169

GO - Molecular functioni

  • clathrin light chain binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9NYX4. 3 interactors.
MINTiMINT-123006.
STRINGi9606.ENSP00000252939.

Structurei

3D structure databases

ProteinModelPortaliQ9NYX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi182 – 188Poly-Ala7

Sequence similaritiesi

Belongs to the NSG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKA7. Eukaryota.
ENOG41118NY. LUCA.
GeneTreeiENSGT00390000000483.
HOGENOMiHOG000030909.
HOVERGENiHBG012403.
InParanoidiQ9NYX4.
KOiK15493.
OMAiCLLIMYK.
OrthoDBiEOG091G0KES.
PhylomeDBiQ9NYX4.
TreeFamiTF332232.

Family and domain databases

InterProiIPR009431. Calcyon_neuron-sp.
[Graphical view]
PfamiPF06387. Calcyon. 1 hit.
[Graphical view]
PIRSFiPIRSF002383. Calcyon. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NYX4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVKLGCSFSG KPGKDPGDQD GAAMDSVPLI SPLDISQLQP PLPDQVVIKT
60 70 80 90 100
QTEYQLSSPD QQNFPDLEGQ RLNCSHPEEG RRLPTARMIA FAMALLGCVL
110 120 130 140 150
IMYKAIWYDQ FTCPDGFLLR HKICTPLTLE MYYTEMDPER HRSILAAIGA
160 170 180 190 200
YPLSRKHGTE TPAAWGDGYR AAKEERKGPT QAGAAAAATE PPGKPSAKAE
210
KEAARKAAGS AAPPPAQ
Length:217
Mass (Da):23,434
Last modified:October 1, 2000 - v1
Checksum:i7B3ACA9042819F58
GO
Isoform 2 (identifier: Q9NYX4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-124: HKIC → GPEF
     125-217: Missing.

Note: Gene prediction based on EST data.
Show »
Length:124
Mass (Da):13,701
Checksum:i5B3D77A683A73CC9
GO
Isoform 3 (identifier: Q9NYX4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-217: LPTARMIAFA...AGSAAPPPAQ → VTTPTPPTVG...RCRLTKQWRS

Note: Gene prediction based on EST data.
Show »
Length:158
Mass (Da):17,547
Checksum:i3451BF975275F836
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03447383 – 217LPTAR…PPPAQ → VTTPTPPTVGTQDRRRRWKA AGGAWVPSRPVWGPLALREE RVAVSHPAQSWPHRLPCTHQ APSRGCRCRLTKQWRS in isoform 3. CuratedAdd BLAST135
Alternative sequenceiVSP_034474121 – 124HKIC → GPEF in isoform 2. Curated4
Alternative sequenceiVSP_034475125 – 217Missing in isoform 2. CuratedAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225903 mRNA. Translation: AAF34714.1.
AL360181 Genomic DNA. Translation: CAH70279.1.
AL360181 Genomic DNA. Translation: CAH70280.1.
AL360181 Genomic DNA. Translation: CAH70281.1.
CH471211 Genomic DNA. Translation: EAW61334.1.
BC038978 mRNA. Translation: AAH38978.1.
CCDSiCCDS7678.1. [Q9NYX4-1]
RefSeqiNP_056537.1. NM_015722.3. [Q9NYX4-1]
UniGeneiHs.148680.

Genome annotation databases

EnsembliENST00000252939; ENSP00000252939; ENSG00000130643. [Q9NYX4-1]
ENST00000368555; ENSP00000357543; ENSG00000130643. [Q9NYX4-3]
ENST00000368558; ENSP00000357546; ENSG00000130643. [Q9NYX4-2]
GeneIDi50632.
KEGGihsa:50632.
UCSCiuc001lmo.3. human. [Q9NYX4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF225903 mRNA. Translation: AAF34714.1.
AL360181 Genomic DNA. Translation: CAH70279.1.
AL360181 Genomic DNA. Translation: CAH70280.1.
AL360181 Genomic DNA. Translation: CAH70281.1.
CH471211 Genomic DNA. Translation: EAW61334.1.
BC038978 mRNA. Translation: AAH38978.1.
CCDSiCCDS7678.1. [Q9NYX4-1]
RefSeqiNP_056537.1. NM_015722.3. [Q9NYX4-1]
UniGeneiHs.148680.

3D structure databases

ProteinModelPortaliQ9NYX4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9NYX4. 3 interactors.
MINTiMINT-123006.
STRINGi9606.ENSP00000252939.

Chemistry databases

DrugBankiDB00714. Apomorphine.
DB00363. Clozapine.
DB00831. Trifluoperazine.

PTM databases

iPTMnetiQ9NYX4.
PhosphoSitePlusiQ9NYX4.

Polymorphism and mutation databases

BioMutaiCALY.
DMDMi17374622.

Proteomic databases

PaxDbiQ9NYX4.
PeptideAtlasiQ9NYX4.
PRIDEiQ9NYX4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252939; ENSP00000252939; ENSG00000130643. [Q9NYX4-1]
ENST00000368555; ENSP00000357543; ENSG00000130643. [Q9NYX4-3]
ENST00000368558; ENSP00000357546; ENSG00000130643. [Q9NYX4-2]
GeneIDi50632.
KEGGihsa:50632.
UCSCiuc001lmo.3. human. [Q9NYX4-1]

Organism-specific databases

CTDi50632.
DisGeNETi50632.
GeneCardsiCALY.
HGNCiHGNC:17938. CALY.
HPAiHPA042283.
MIMi604647. gene.
neXtProtiNX_Q9NYX4.
OpenTargetsiENSG00000130643.
PharmGKBiPA162380987.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKA7. Eukaryota.
ENOG41118NY. LUCA.
GeneTreeiENSGT00390000000483.
HOGENOMiHOG000030909.
HOVERGENiHBG012403.
InParanoidiQ9NYX4.
KOiK15493.
OMAiCLLIMYK.
OrthoDBiEOG091G0KES.
PhylomeDBiQ9NYX4.
TreeFamiTF332232.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130643-MONOMER.

Miscellaneous databases

GeneWikiiDRD1IP.
GenomeRNAii50632.
PROiQ9NYX4.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130643.
CleanExiHS_CALY.
GenevisibleiQ9NYX4. HS.

Family and domain databases

InterProiIPR009431. Calcyon_neuron-sp.
[Graphical view]
PfamiPF06387. Calcyon. 1 hit.
[Graphical view]
PIRSFiPIRSF002383. Calcyon. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCALY_HUMAN
AccessioniPrimary (citable) accession number: Q9NYX4
Secondary accession number(s): Q5VWX3, Q5VWY5, Q5VWY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally thought to interact with the D1 dopamine receptor (DRD1) and to play a role in potentiating calcium ion-dependent signaling but this work was later retracted.1 Publication
In cerebrospinal fluid (CSF), found to be O-glycosylated in the predicted intracellular region at Thr-180 and Thr-189 (PubMed:19838169). This glycosylation has been confirmed by a separate mass spectrometry (MS) method (PubMed:23234360). Glycosylation in this region of the protein is unexplained as yet.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.