Q9NYX4 (CALY_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuron-specific vesicular protein calcyon

Gene names

Name:	CALY
Synonyms:	DRD1IP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis. Ref.9
Subunit structure	Interacts with CLTA. Ref.9
Subcellular location	Cytoplasmic vesicle membrane; Single-pass membrane protein. Cell membrane; Single-pass membrane protein Ref.7 Ref.9.
Tissue specificity	Expressed in the pyramidal cells of the prefrontal cortex, in hippothalamus and in caudate nucleus. No expression in spleen. Up-regulated in the prefrontal cortex of schizophrenic patients with nearly twice the levels of non-schizophrenics. Ref.6
Post-translational modification	Glycosylated Probable.
Sequence similarities	Belongs to the NSG family.
Caution	Was originally (Ref.1) thought to interact with the D1 dopamine receptor (DRD1) and to play a role in potentiating calcium ion-dependent signaling but this work was later retracted.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	clathrin coat assembly Inferred from direct assay Ref.9. Source: UniProtKB dopamine receptor signaling pathway Inferred from electronic annotation. Source: InterPro endocytosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of endocytosis Inferred from mutant phenotype Ref.9. Source: UniProtKB
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Inferred from direct assay Ref.7. Source: UniProtKB
Molecular function	clathrin light chain binding Inferred from direct assay Ref.9. Source: UniProtKB dopamine receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NYX4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NYX4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     121-124: HKIC → GPEF
     125-217: Missing.

Note: Gene prediction based on EST data.

Isoform 3 (identifier: Q9NYX4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     83-217: LPTARMIAFA...AGSAAPPPAQ → VTTPTPPTVG...RCRLTKQWRS

Note: Gene prediction based on EST data.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	Neuron-specific vesicular protein calcyon		PRO_0000164368
Regions
	Topological domain	1 – 87	87	Extracellular Potential
	Transmembrane	88 – 108	21	Helical; Potential
	Topological domain	109 – 217	109	Cytoplasmic Potential
	Compositional bias	182 – 188	7	Poly-Ala
Amino acid modifications
	Modified residue	151	1	Phosphotyrosine Ref.8
	Glycosylation	73	1	N-linked (GlcNAc...) Probable
Natural variations
	Alternative sequence	83 – 217	135	LPTAR…PPPAQ → VTTPTPPTVGTQDRRRRWKA AGGAWVPSRPVWGPLALREE RVAVSHPAQSWPHRLPCTHQ APSRGCRCRLTKQWRS in isoform 3.		VSP_034473
	Alternative sequence	121 – 124	4	HKIC → GPEF in isoform 2.		VSP_034474
	Alternative sequence	125 – 217	93	Missing in isoform 2.		VSP_034475

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7B3ACA9042819F58	FASTA	217	23,434
	10 20 30 40 50 60 MVKLGCSFSG KPGKDPGDQD GAAMDSVPLI SPLDISQLQP PLPDQVVIKT QTEYQLSSPD 70 80 90 100 110 120 QQNFPDLEGQ RLNCSHPEEG RRLPTARMIA FAMALLGCVL IMYKAIWYDQ FTCPDGFLLR 130 140 150 160 170 180 HKICTPLTLE MYYTEMDPER HRSILAAIGA YPLSRKHGTE TPAAWGDGYR AAKEERKGPT 190 200 210 QAGAAAAATE PPGKPSAKAE KEAARKAAGS AAPPPAQ
	Isoform 2 [UniParc]. Checksum: 5B3D77A683A73CC9	FASTA	124	13,701
	10 20 30 40 50 60 MVKLGCSFSG KPGKDPGDQD GAAMDSVPLI SPLDISQLQP PLPDQVVIKT QTEYQLSSPD 70 80 90 100 110 120 QQNFPDLEGQ RLNCSHPEEG RRLPTARMIA FAMALLGCVL IMYKAIWYDQ FTCPDGFLLR GPEF
	Isoform 3 [UniParc]. Checksum: 3451BF975275F836	FASTA	158	17,547
	10 20 30 40 50 60 MVKLGCSFSG KPGKDPGDQD GAAMDSVPLI SPLDISQLQP PLPDQVVIKT QTEYQLSSPD 70 80 90 100 110 120 QQNFPDLEGQ RLNCSHPEEG RRVTTPTPPT VGTQDRRRRW KAAGGAWVPS RPVWGPLALR 130 140 150 EERVAVSHPA QSWPHRLPCT HQAPSRGCRC RLTKQWRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dual signaling regulated by calcyon, a D1 dopamine receptor interacting protein." Lezcano N., Mrzljak L., Eubanks S., Levenson R., Goldman-Rakic P., Bergson C. Science 287:1660-1664(2000) [PubMed: 10698743] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Lezcano N., Mrzljak L., Levenson R., Bergson C. Science 314:1681-1681(2006) [PubMed: 17170272] [Abstract] Cited for: RETRACTION.
[3]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Up-regulation of the D1 dopamine receptor-interacting protein, calcyon, in patients with schizophrenia." Koh P.O., Bergson C., Undie A.S., Goldman-Rakic P.S., Lidow M.S. Arch. Gen. Psychiatry 60:311-319(2003) [PubMed: 12622665] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Elevated intracellular calcium triggers recruitment of the receptor cross-talk accessory protein calcyon to the plasma membrane." Ali M.K., Bergson C. J. Biol. Chem. 278:51654-51663(2003) [PubMed: 14534309] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-151, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis." Xiao J., Dai R., Negyessy L., Bergson C. J. Biol. Chem. 281:15182-15193(2006) [PubMed: 16595675] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CLTA.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF225903 mRNA. Translation: AAF34714.1. AL360181 Genomic DNA. Translation: CAH70279.1. AL360181 Genomic DNA. Translation: CAH70280.1. AL360181 Genomic DNA. Translation: CAH70281.1. CH471211 Genomic DNA. Translation: EAW61334.1. BC038978 mRNA. Translation: AAH38978.1.
IPI	IPI00024587. IPI00477695. IPI00647166.
RefSeq	NP_056537.1. NM_015722.3.
UniGene	Hs.148680.
3D structure databases
ProteinModelPortal	Q9NYX4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9NYX4.
Polymorphism databases
DMDM	17374622.
Proteomic databases
PRIDE	Q9NYX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000252939; ENSP00000252939; ENSG00000130643.
GeneID	50632.
KEGG	hsa:50632.
UCSC	uc001lmo.2. human.
Organism-specific databases
CTD	50632.
GeneCards	GC10M135138.
H-InvDB	HIX0025917.
HGNC	HGNC:17938. CALY.
HPA	HPA042283.
MIM	604647. gene.
neXtProt	NX_Q9NYX4.
PharmGKB	PA162380987.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG13794.
GeneTree	ENSGT00390000000483.
HOGENOM	HBG713191.
HOVERGEN	HBG012403.
InParanoid	Q9NYX4.
OMA	SCPDGFL.
OrthoDB	EOG41C6XC.
PhylomeDB	Q9NYX4.
Gene expression databases
ArrayExpress	Q9NYX4.
Bgee	Q9NYX4.
CleanEx	HS_CALY.
Genevestigator	Q9NYX4.
GermOnline	ENSG00000130643. Homo sapiens.
Family and domain databases
InterPro	IPR009431. D1-dopamine_rcpt_interact. [Graphical view]
KO	K15493.
Pfam	PF06387. Calcyon. 1 hit. [Graphical view]
PIRSF	PIRSF002383. Calcyon. 1 hit.
ProtoNet	Search...
Other
DrugBank	DB00714. Apomorphine. DB00363. Clozapine. DB00875. Flupenthixol. DB00831. Trifluoperazine.
NextBio	53168.
SOURCE	Search...

Entry information

Entry name

CALY_HUMAN

Accession

Primary (citable) accession number: Q9NYX4
Secondary accession number(s): Q5VWX3, Q5VWY5, Q5VWY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.