Reviewed, UniProtKB/Swiss-Prot Q9NYW8 (RBAK_HUMAN)

Names and origin

Protein names

Recommended name:
    RB-associated KRAB zinc finger protein
Alternative name(s):
    RB-associated KRAB repressor
      Short name=hRBaK
    Zinc finger protein 769

Gene names

Name:	RBAK
Synonyms:	ZNF769

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May repress E2F-dependent transcription. May promote AR-dependent transcription. Ref.1 Ref.6
Subunit structure	Interacts with AR and RB1. May also interact with other nuclear hormone receptors such as NR3C1/GR. Ref.1 Ref.6
Subcellular location	Nucleus. Ref.1
Tissue specificity	Expressed in bone, brain, heart, kidney, liver, lung, pancreas and placenta. Ref.1
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 16 C2H2-type zinc fingers. Contains 1 KRAB domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Repressor
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Ref.1 Traceable author statement. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.1 Traceable author statement. Source: UniProtKB
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro transcription repressor activity Ref.1 Traceable author statement. Source: UniProtKB zinc ion binding Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	RB-associated KRAB zinc finger protein		PRO_0000316976
Regions
	Domain	8 – 79	72	KRAB
	Zinc finger	261 – 283	23	C2H2-type 1
	Zinc finger	289 – 311	23	C2H2-type 2
	Zinc finger	317 – 339	23	C2H2-type 3
	Zinc finger	345 – 367	23	C2H2-type 4
	Zinc finger	373 – 395	23	C2H2-type 5
	Zinc finger	401 – 423	23	C2H2-type 6
	Zinc finger	429 – 451	23	C2H2-type 7
	Zinc finger	457 – 479	23	C2H2-type 8
	Zinc finger	485 – 505	21	C2H2-type 9
	Zinc finger	511 – 533	23	C2H2-type 10; degenerate
	Zinc finger	539 – 561	23	C2H2-type 11
	Zinc finger	567 – 589	23	C2H2-type 12
	Zinc finger	595 – 617	23	C2H2-type 13
	Zinc finger	623 – 645	23	C2H2-type 14
	Zinc finger	651 – 673	23	C2H2-type 15
	Zinc finger	679 – 701	23	C2H2-type 16
	Region	171 – 260	90	Required for interaction with RB1
	Region	417 – 714	298	Interaction with AR
Natural variations
	Natural variant	229	1	G → E: dbSNP rs35352738.		VAR_038438
Experimental info
	Sequence conflict	273	1	K → E in BAF85758. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9NYW8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 36D90A6206118F8B	FASTA	714	82,995
	10 20 30 40 50 60 MNTLQGPVSF KDVAVDFTQE EWQQLDPDEK ITYRDVMLEN YSHLVSVGYD TTKPNVIIKL 70 80 90 100 110 120 EQGEEPWIMG GEFPCQHSPE AWRVDDLIER IQENEDKHSR QAACINSKTL TEEKENTFSQ 130 140 150 160 170 180 IYMETSLVPS SIIAHNCVSC GKNLESISQL ISSDGSYART KPDECNECGK TYHGEKMCEF 190 200 210 220 230 240 NQNGDTYSHN EENILQKISI LEKPFEYNEC MEALDNEAVF IAHKRAYIGE KPYEWNDSGP 250 260 270 280 290 300 DFIQMSNFNA YQRSQMEMKP FECSECGKSF CKKSKFIIHQ RAHTGEKPYE CNVCGKSFSQ 310 320 330 340 350 360 KGTLTVHRRS HLEEKPYKCN ECGKTFCQKL HLTQHLRTHS GEKPYECSEC GKTFCQKTHL 370 380 390 400 410 420 TLHQRNHSGE RPYPCNECGK SFSRKSALSD HQRTHTGEKL YKCNECGKSY YRKSTLITHQ 430 440 450 460 470 480 RTHTGEKPYQ CSECGKFFSR VSYLTIHYRS HLEEKPYECN ECGKTFNLNS AFIRHRKVHT 490 500 510 520 530 540 EEKSHECSEC GKFSQLYLTD HHTAHLEEKP YECNECGKTF LVNSAFDGHQ PLPKGEKSYE 550 560 570 580 590 600 CNVCGKLFNE LSYYTEHYRS HSEEKPYGCS ECGKTFSHNS SLFRHQRVHT GEKPYECYEC 610 620 630 640 650 660 GKFFSQKSYL TIHHRIHSGE KPYECSKCGK VFSRMSNLTV HYRSHSGEKP YECNECGKVF 670 680 690 700 710 SQKSYLTVHY RTHSGEKPYE CNECGKKFHH RSAFNSHQRI HRRGNMNVLD VENL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a novel Kruppel-associated box family transcriptional repressor that interacts with the retinoblastoma gene product, RB." Skapek S.X., Jansen D., Wei T.-F., McDermott T., Huang W., Olson E.N., Lee E.Y.-H.P. J. Biol. Chem. 275:7212-7223(2000) [PubMed: 10702291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RB1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed: 12853948] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"The retinoblastoma protein-associated transcription repressor RBaK interacts with the androgen receptor and enhances its transcriptional activity." Hofman K., Swinnen J.V., Claessens F., Verhoeven G., Heyns W. J. Mol. Endocrinol. 31:583-596(2003) [PubMed: 14664718] [Abstract] Cited for: FUNCTION, INTERACTION WITH AR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF226869 mRNA. Translation: AAF43389.1. AK293069 mRNA. Translation: BAF85758.1. AC092032 Genomic DNA. Translation: AAQ93364.1. AC008167 Genomic DNA. No translation available. CH471144 Genomic DNA. Translation: EAW87321.1. BC136676 mRNA. Translation: AAI36677.1.
IPI	IPI00302755.
RefSeq	NP_066986.1.
UniGene	Hs.396178
3D structure databases
HSSP	HSSP built from PDB template 1ZNF based on UniProtKB P08045.
ModBase	Search...
PTM databases
PhosphoSite	Q9NYW8.
Proteomic databases
PRIDE	Q9NYW8.
Genome annotation databases
Ensembl	ENSG00000146587. Homo sapiens. [Contig view]
GeneID	57786.
KEGG	hsa:57786.
Organism-specific databases
GeneCards	GC07P004990.
HGNC	HGNC:17680. RBAK.
MIM	608191. gene.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q9NYW8.
HOVERGEN	Q9NYW8.
Gene expression databases
ArrayExpress	Q9NYW8.
Bgee	Q9NYW8.
CleanEx	HS_RBAK.
Family and domain databases
InterPro	IPR001909. Krueppel-associated_box. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 14 hits.
Pfam	PF01352. KRAB. 1 hit. PF00096. zf-C2H2. 14 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 14 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00349. KRAB. 1 hit. SM00355. ZnF_C2H2. 16 hits. [Graphical view]
PROSITE	PS50805. KRAB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 14 hits. PS50157. ZINC_FINGER_C2H2_2. 15 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	64717.
SOURCE	Search...

Entry information

Entry name

RBAK_HUMAN

Accession

Primary (citable) accession number: Q9NYW8
Secondary accession number(s): A6NDF2, A8KAK4, B2RN44

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)