Q9NYW6 (TA2R3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taste receptor type 2 member 3
Short name=T2R3

Gene names

Name:

TAS2R3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Gustducin-coupled receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. Ref.1 Ref.7
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in the antrum and fundus (part of the stomach), duodenum and in gastric endocrine cells. Ref.1
Miscellaneous	Several bitter taste receptors are expressed in a single taste receptor cell.
Sequence similarities	Belongs to the G-protein coupled receptor T2R family.
Sequence caution	The sequence AAU21157.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred by curator Ref.1. Source: UniProtKB
Molecular_function	taste receptor activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Taste receptor type 2 member 3		PRO_0000082197
Regions
	Topological domain	1 – 6	6	Extracellular Potential
	Transmembrane	7 – 27	21	Helical; Name=1; Potential
	Topological domain	28 – 42	15	Cytoplasmic Potential
	Transmembrane	43 – 63	21	Helical; Name=2; Potential
	Topological domain	64 – 94	31	Extracellular Potential
	Transmembrane	95 – 115	21	Helical; Name=3; Potential
	Topological domain	116 – 128	13	Cytoplasmic Potential
	Transmembrane	129 – 149	21	Helical; Name=4; Potential
	Topological domain	150 – 186	37	Extracellular Potential
	Transmembrane	187 – 207	21	Helical; Name=5; Potential
	Topological domain	208 – 234	27	Cytoplasmic Potential
	Transmembrane	235 – 255	21	Helical; Name=6; Potential
	Topological domain	256 – 266	11	Extracellular Potential
	Transmembrane	267 – 287	21	Helical; Name=7; Potential
	Topological domain	288 – 316	29	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	166	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9NYW6 [UniParc]. Last modified September 5, 2006. Version 3. Checksum: 6A7BA3C6497ABC5B	FASTA	316	35,915
	10 20 30 40 50 60 MMGLTEGVFL ILSGTQFTLG ILVNCFIELV NGSSWFKTKR MSLSDFIITT LALLRIILLC 70 80 90 100 110 120 IILTDSFLIE FSPNTHDSGI IMQIIDVSWT FTNHLSIWLA TCLGVLYCLK IASFSHPTFL 130 140 150 160 170 180 WLKWRVSRVM VWMLLGALLL SCGSTASLIN EFKLYSVFRG IEATRNVTEH FRKKRSEYYL 190 200 210 220 230 240 IHVLGTLWYL PPLIVSLASY SLLIFSLGRH TRQMLQNGTS SRDPTTEAHK RAIRIILSFF 250 260 270 280 290 300 FLFLLYFLAF LIASFGNFLP KTKMAKMIGE VMTMFYPAGH SFILILGNSK LKQTFVVMLR 310 CESGHLKPGS KGPIFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel family of mammalian taste receptors." Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., Zuker C.S. Cell 100:693-702(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"Evolution of bitter taste receptors in humans and apes." Fischer A., Gilad Y., Man O., Paeaebo S. Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"T2Rs function as bitter taste receptors." Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., Zuker C.S., Ryba N.J.P. Cell 100:703-711(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Receptors for bitter and sweet taste." Montmayeur J.-P., Matsunami H. Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[9]	"Molecular mechanisms of bitter and sweet taste transduction." Margolskee R.F. J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[10]	"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J. Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF227130 Genomic DNA. Translation: AAF43903.1. AY724961 Genomic DNA. Translation: AAU21157.1. Different initiation. AC004979 Genomic DNA. Translation: AAS02038.1. BC069337 mRNA. Translation: AAH69337.1. CH236950 Genomic DNA. Translation: EAL24017.1. CH471070 Genomic DNA. Translation: EAW83982.1. BC095523 mRNA. Translation: AAH95523.1.
IPI	IPI00027205.
RefSeq	NP_058639.1. NM_016943.2.
UniGene	Hs.676011.
3D structure databases
ProteinModelPortal	Q9NYW6.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9NYW6.
Polymorphism databases
DMDM	114152888.
Proteomic databases
PaxDb	Q9NYW6.
PRIDE	Q9NYW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000247879; ENSP00000247879; ENSG00000127362.
GeneID	50831.
KEGG	hsa:50831.
UCSC	uc003vwp.1. human.
Organism-specific databases
CTD	50831.
GeneCards	GC07P141463.
HGNC	HGNC:14910. TAS2R3.
MIM	604868. gene.
neXtProt	NX_Q9NYW6.
PharmGKB	PA37922.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG27838.
HOGENOM	HOG000234595.
HOVERGEN	HBG060698.
InParanoid	Q9NYW6.
KO	K08474.
OMA	DSGIIMQ.
OrthoDB	EOG4CJVHP.
PhylomeDB	Q9NYW6.
Gene expression databases
ArrayExpress	Q9NYW6.
Bgee	Q9NYW6.
CleanEx	HS_TAS2R3.
Genevestigator	Q9NYW6.
GermOnline	ENSG00000127362. Homo sapiens.
Family and domain databases
InterPro	IPR007960. TAS2_rcpt. [Graphical view]
Pfam	PF05296. TAS2R. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	50831.
NextBio	53273.
SOURCE	Search...

Entry information

Entry name

TA2R3_HUMAN

Accession

Primary (citable) accession number: Q9NYW6
Secondary accession number(s): A4D1U2, Q645W2, Q75MV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	September 5, 2006
Last modified:	April 3, 2013
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.