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Protein

Taste receptor type 2 member 4

Gene

TAS2R4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Gustducin-coupled receptor for denatonium and N(6)-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. In airway epithelial cells, binding of denatonium increases the intracellular calcium ion concentration and stimulates ciliary beat frequency.1 Publication

GO - Molecular functioni

  1. bitter taste receptor activity Source: UniProtKB
  2. taste receptor activity Source: UniProtKB

GO - Biological processi

  1. detection of chemical stimulus involved in sensory perception of bitter taste Source: UniProtKB
  2. respiratory gaseous exchange Source: Ensembl
  3. sensory perception of taste Source: ProtInc
  4. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Sensory transduction, Taste

Enzyme and pathway databases

ReactomeiREACT_18319. Class C/3 (Metabotropic glutamate/pheromone receptors).
REACT_19231. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Taste receptor type 2 member 4
Short name:
T2R4
Gene namesi
Name:TAS2R4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:14911. TAS2R4.

Subcellular locationi

Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell projectioncilium membrane 1 Publication
Note: In airway epithelial cells, localizes to motile cilia.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99ExtracellularSequence Analysis
Transmembranei10 – 3021Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini31 – 4616CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei47 – 6721Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini68 – 8114ExtracellularSequence AnalysisAdd
BLAST
Transmembranei82 – 10221Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini103 – 13129CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei132 – 15221Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini153 – 17220ExtracellularSequence AnalysisAdd
BLAST
Transmembranei173 – 19321Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini194 – 23037CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei231 – 25121Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini252 – 26211ExtracellularSequence AnalysisAdd
BLAST
Transmembranei263 – 28321Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini284 – 29916CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. ciliary membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37923.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Taste receptor type 2 member 4PRO_0000082205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9NYW5.
PRIDEiQ9NYW5.

Expressioni

Tissue specificityi

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed on airway ciliated epithelium.1 Publication

Gene expression databases

BgeeiQ9NYW5.
CleanExiHS_TAS2R4.
ExpressionAtlasiQ9NYW5. baseline.
GenevestigatoriQ9NYW5.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000247881.

Structurei

3D structure databases

ProteinModelPortaliQ9NYW5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG42218.
GeneTreeiENSGT00760000119096.
HOGENOMiHOG000126900.
HOVERGENiHBG098740.
InParanoidiQ9NYW5.
KOiK08474.
OMAiVERSVYL.
OrthoDBiEOG7HXCR9.
PhylomeDBiQ9NYW5.
TreeFamiTF335891.

Family and domain databases

InterProiIPR007960. T2R.
IPR030055. TAS2R4.
[Graphical view]
PANTHERiPTHR11394. PTHR11394. 1 hit.
PTHR11394:SF55. PTHR11394:SF55. 1 hit.
PfamiPF05296. TAS2R. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NYW5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRLFYFSAI IASVILNFVG IIMNLFITVV NCKTWVKSHR ISSSDRILFS
60 70 80 90 100
LGITRFLMLG LFLVNTIYFV SSNTERSVYL SAFFVLCFMF LDSSSVWFVT
110 120 130 140 150
LLNILYCVKI TNFQHSVFLL LKRNISPKIP RLLLACVLIS AFTTCLYITL
160 170 180 190 200
SQASPFPELV TTRNNTSFNI SEGILSLVVS LVLSSSLQFI INVTSASLLI
210 220 230 240 250
HSLRRHIQKM QKNATGFWNP QTEAHVGAMK LMVYFLILYI PYSVATLVQY
260 270 280 290
LPFYAGMDMG TKSICLIFAT LYSPGHSVLI IITHPKLKTT AKKILCFKK
Length:299
Mass (Da):33,841
Last modified:October 1, 2000 - v1
Checksum:i8265610092B19945
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31R → Q.
Corresponds to variant rs2233995 [ dbSNP | Ensembl ].
VAR_034535
Natural varianti7 – 71F → S.
Corresponds to variant rs2233998 [ dbSNP | Ensembl ].
VAR_034536
Natural varianti62 – 621F → L.
Corresponds to variant rs2233999 [ dbSNP | Ensembl ].
VAR_053341
Natural varianti74 – 741T → M.
Corresponds to variant rs2234000 [ dbSNP | Ensembl ].
VAR_020200
Natural varianti96 – 961V → L.
Corresponds to variant rs2234001 [ dbSNP | Ensembl ].
VAR_020201
Natural varianti171 – 1711S → N.
Corresponds to variant rs2234002 [ dbSNP | Ensembl ].
VAR_020202
Natural varianti191 – 1911I → V.
Corresponds to variant rs2234003 [ dbSNP | Ensembl ].
VAR_053342

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF227131 Genomic DNA. Translation: AAF43904.1.
AY724958 Genomic DNA. Translation: AAU21154.1.
AC004979 Genomic DNA. Translation: AAS02039.1.
CCDSiCCDS5868.1.
RefSeqiNP_058640.1. NM_016944.1.
UniGeneiHs.735690.

Genome annotation databases

EnsembliENST00000247881; ENSP00000247881; ENSG00000127364.
GeneIDi50832.
KEGGihsa:50832.
UCSCiuc003vwq.1. human.

Polymorphism databases

DMDMi29839665.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

A tail of protection - Issue 119 of July 2010

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF227131 Genomic DNA. Translation: AAF43904.1.
AY724958 Genomic DNA. Translation: AAU21154.1.
AC004979 Genomic DNA. Translation: AAS02039.1.
CCDSiCCDS5868.1.
RefSeqiNP_058640.1. NM_016944.1.
UniGeneiHs.735690.

3D structure databases

ProteinModelPortaliQ9NYW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000247881.

Protein family/group databases

GPCRDBiSearch...

Polymorphism databases

DMDMi29839665.

Proteomic databases

PaxDbiQ9NYW5.
PRIDEiQ9NYW5.

Protocols and materials databases

DNASUi50832.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000247881; ENSP00000247881; ENSG00000127364.
GeneIDi50832.
KEGGihsa:50832.
UCSCiuc003vwq.1. human.

Organism-specific databases

CTDi50832.
GeneCardsiGC07P141479.
HGNCiHGNC:14911. TAS2R4.
MIMi604869. gene.
neXtProtiNX_Q9NYW5.
PharmGKBiPA37923.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG42218.
GeneTreeiENSGT00760000119096.
HOGENOMiHOG000126900.
HOVERGENiHBG098740.
InParanoidiQ9NYW5.
KOiK08474.
OMAiVERSVYL.
OrthoDBiEOG7HXCR9.
PhylomeDBiQ9NYW5.
TreeFamiTF335891.

Enzyme and pathway databases

ReactomeiREACT_18319. Class C/3 (Metabotropic glutamate/pheromone receptors).
REACT_19231. G alpha (i) signalling events.

Miscellaneous databases

GeneWikiiTAS2R4.
GenomeRNAii50832.
NextBioi53277.
PROiQ9NYW5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NYW5.
CleanExiHS_TAS2R4.
ExpressionAtlasiQ9NYW5. baseline.
GenevestigatoriQ9NYW5.

Family and domain databases

InterProiIPR007960. T2R.
IPR030055. TAS2R4.
[Graphical view]
PANTHERiPTHR11394. PTHR11394. 1 hit.
PTHR11394:SF55. PTHR11394:SF55. 1 hit.
PfamiPF05296. TAS2R. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TOPOLOGY.
  2. "Evolution of bitter taste receptors in humans and apes."
    Fischer A., Gilad Y., Man O., Paeaebo S.
    Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: CHARACTERIZATION.
  5. Cited for: REVIEW.
  6. "Molecular mechanisms of bitter and sweet taste transduction."
    Margolskee R.F.
    J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. "Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
    Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
    Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  8. "Motile cilia of human airway epithelia are chemosensory."
    Shah A.S., Ben-Shahar Y., Moninger T.O., Kline J.N., Welsh M.J.
    Science 325:1131-1134(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiTA2R4_HUMAN
AccessioniPrimary (citable) accession number: Q9NYW5
Secondary accession number(s): Q645W5, Q75MV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Several bitter taste receptors are expressed in a single taste receptor cell.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.