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Q9NYW5 (TA2R4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 2 member 4

Short name=T2R4
Gene names
Name:TAS2R4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Gustducin-coupled receptor for denatonium and N(6)-propyl-2-thiouracil implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5. In airway epithelial cells, binding of denatonium increases the intracellular calcium ion concentration and stimulates ciliary beat frequency. Ref.8

Subcellular location

Membrane; Multi-pass membrane protein. Cell projectioncilium membrane. Note: In airway epithelial cells, localizes to motile cilia. Ref.8

Tissue specificity

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed on airway ciliated epithelium. Ref.8

Miscellaneous

Several bitter taste receptors are expressed in a single taste receptor cell.

Sequence similarities

Belongs to the G-protein coupled receptor T2R family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Taste receptor type 2 member 4
PRO_0000082205

Regions

Topological domain1 – 99Extracellular Potential
Transmembrane10 – 3021Helical; Name=1; Potential
Topological domain31 – 4616Cytoplasmic Potential
Transmembrane47 – 6721Helical; Name=2; Potential
Topological domain68 – 8114Extracellular Potential
Transmembrane82 – 10221Helical; Name=3; Potential
Topological domain103 – 13129Cytoplasmic Potential
Transmembrane132 – 15221Helical; Name=4; Potential
Topological domain153 – 17220Extracellular Potential
Transmembrane173 – 19321Helical; Name=5; Potential
Topological domain194 – 23037Cytoplasmic Potential
Transmembrane231 – 25121Helical; Name=6; Potential
Topological domain252 – 26211Extracellular Potential
Transmembrane263 – 28321Helical; Name=7; Potential
Topological domain284 – 29916Cytoplasmic Potential

Amino acid modifications

Glycosylation1641N-linked (GlcNAc...) Potential
Glycosylation1651N-linked (GlcNAc...) Potential
Glycosylation1691N-linked (GlcNAc...) Potential

Natural variations

Natural variant31R → Q.
Corresponds to variant rs2233995 [ dbSNP | Ensembl ].
VAR_034535
Natural variant71F → S.
Corresponds to variant rs2233998 [ dbSNP | Ensembl ].
VAR_034536
Natural variant621F → L.
Corresponds to variant rs2233999 [ dbSNP | Ensembl ].
VAR_053341
Natural variant741T → M.
Corresponds to variant rs2234000 [ dbSNP | Ensembl ].
VAR_020200
Natural variant961V → L.
Corresponds to variant rs2234001 [ dbSNP | Ensembl ].
VAR_020201
Natural variant1711S → N.
Corresponds to variant rs2234002 [ dbSNP | Ensembl ].
VAR_020202
Natural variant1911I → V.
Corresponds to variant rs2234003 [ dbSNP | Ensembl ].
VAR_053342

Sequences

Sequence LengthMass (Da)Tools
Q9NYW5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 8265610092B19945

FASTA29933,841
        10         20         30         40         50         60 
MLRLFYFSAI IASVILNFVG IIMNLFITVV NCKTWVKSHR ISSSDRILFS LGITRFLMLG 

        70         80         90        100        110        120 
LFLVNTIYFV SSNTERSVYL SAFFVLCFMF LDSSSVWFVT LLNILYCVKI TNFQHSVFLL 

       130        140        150        160        170        180 
LKRNISPKIP RLLLACVLIS AFTTCLYITL SQASPFPELV TTRNNTSFNI SEGILSLVVS 

       190        200        210        220        230        240 
LVLSSSLQFI INVTSASLLI HSLRRHIQKM QKNATGFWNP QTEAHVGAMK LMVYFLILYI 

       250        260        270        280        290 
PYSVATLVQY LPFYAGMDMG TKSICLIFAT LYSPGHSVLI IITHPKLKTT AKKILCFKK 

« Hide

References

« Hide 'large scale' references
[1]"A novel family of mammalian taste receptors."
Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., Zuker C.S.
Cell 100:693-702(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TOPOLOGY.
[2]"Evolution of bitter taste receptors in humans and apes."
Fischer A., Gilad Y., Man O., Paeaebo S.
Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"T2Rs function as bitter taste receptors."
Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., Zuker C.S., Ryba N.J.P.
Cell 100:703-711(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Receptors for bitter and sweet taste."
Montmayeur J.-P., Matsunami H.
Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[6]"Molecular mechanisms of bitter and sweet taste transduction."
Margolskee R.F.
J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[7]"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[8]"Motile cilia of human airway epithelia are chemosensory."
Shah A.S., Ben-Shahar Y., Moninger T.O., Kline J.N., Welsh M.J.
Science 325:1131-1134(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Web resources

Protein Spotlight

A tail of protection - Issue 119 of July 2010

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF227131 Genomic DNA. Translation: AAF43904.1.
AY724958 Genomic DNA. Translation: AAU21154.1.
AC004979 Genomic DNA. Translation: AAS02039.1.
RefSeqNP_058640.1. NM_016944.1.
UniGeneHs.735690.

3D structure databases

ProteinModelPortalQ9NYW5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000247881.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM29839665.

Proteomic databases

PaxDbQ9NYW5.
PRIDEQ9NYW5.

Protocols and materials databases

DNASU50832.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000247881; ENSP00000247881; ENSG00000127364.
GeneID50832.
KEGGhsa:50832.
UCSCuc003vwq.1. human.

Organism-specific databases

CTD50832.
GeneCardsGC07P141479.
HGNCHGNC:14911. TAS2R4.
MIM604869. gene.
neXtProtNX_Q9NYW5.
PharmGKBPA37923.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42218.
HOGENOMHOG000126900.
HOVERGENHBG098740.
InParanoidQ9NYW5.
KOK08474.
OMAVERSVYL.
OrthoDBEOG7HXCR9.
PhylomeDBQ9NYW5.
TreeFamTF335891.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressQ9NYW5.
BgeeQ9NYW5.
CleanExHS_TAS2R4.
GenevestigatorQ9NYW5.

Family and domain databases

InterProIPR007960. TAS2_rcpt.
[Graphical view]
PfamPF05296. TAS2R. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAS2R4.
GenomeRNAi50832.
NextBio53277.
PROQ9NYW5.
SOURCESearch...

Entry information

Entry nameTA2R4_HUMAN
AccessionPrimary (citable) accession number: Q9NYW5
Secondary accession number(s): Q645W5, Q75MV8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries