ID   TA2R5_HUMAN             Reviewed;         299 AA.
AC   Q9NYW4; Q645W0; Q75MV7;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   24-JAN-2024, entry version 165.
DE   RecName: Full=Taste receptor type 2 member 5;
DE            Short=T2R5;
GN   Name=TAS2R5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TOPOLOGY.
RX   PubMed=10761934; DOI=10.1016/s0092-8674(00)80705-9;
RA   Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P.,
RA   Zuker C.S.;
RT   "A novel family of mammalian taste receptors.";
RL   Cell 100:693-702(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15496549; DOI=10.1093/molbev/msi027;
RA   Fischer A., Gilad Y., Man O., Paeaebo S.;
RT   "Evolution of bitter taste receptors in humans and apes.";
RL   Mol. Biol. Evol. 22:432-436(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=10761935; DOI=10.1016/s0092-8674(00)80706-0;
RA   Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W.,
RA   Zuker C.S., Ryba N.J.P.;
RT   "T2Rs function as bitter taste receptors.";
RL   Cell 100:703-711(2000).
RN   [6]
RP   REVIEW.
RX   PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA   Montmayeur J.-P., Matsunami H.;
RT   "Receptors for bitter and sweet taste.";
RL   Curr. Opin. Neurobiol. 12:366-371(2002).
RN   [7]
RP   REVIEW.
RX   PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA   Margolskee R.F.;
RT   "Molecular mechanisms of bitter and sweet taste transduction.";
RL   J. Biol. Chem. 277:1-4(2002).
RN   [8]
RP   REVIEW.
RX   PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA   Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA   Zuker C.S., Ryba N.J.;
RT   "Coding of sweet, bitter, and umami tastes: different receptor cells
RT   sharing similar signaling pathways.";
RL   Cell 112:293-301(2003).
CC   -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC       and is gustducin-linked. May play a role in sensing the chemical
CC       composition of the gastrointestinal content. The activity of this
CC       receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC       and lead to the gating of TRPM5.
CC   -!- INTERACTION:
CC       Q9NYW4; P13236: CCL4; NbExp=3; IntAct=EBI-17933167, EBI-2873970;
CC       Q9NYW4; Q5QGT7: RTP2; NbExp=3; IntAct=EBI-17933167, EBI-10244780;
CC       Q9NYW4; O95562: SFT2D2; NbExp=3; IntAct=EBI-17933167, EBI-4402330;
CC       Q9NYW4; Q9BRI3: SLC30A2; NbExp=3; IntAct=EBI-17933167, EBI-8644112;
CC       Q9NYW4; Q14656: TMEM187; NbExp=3; IntAct=EBI-17933167, EBI-13046724;
CC       Q9NYW4; O95183: VAMP5; NbExp=3; IntAct=EBI-17933167, EBI-10191195;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC       tongue and palate epithelium and exclusively in gustducin-positive
CC       cells.
CC   -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC       bitter compounds; individual taste cells can discriminate among bitter
CC       stimuli.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF227132; AAF43905.1; -; Genomic_DNA.
DR   EMBL; AY724966; AAU21159.1; -; Genomic_DNA.
DR   EMBL; AC004979; AAS02040.1; -; Genomic_DNA.
DR   EMBL; BC069509; AAH69509.1; -; mRNA.
DR   EMBL; BC101586; AAI01587.1; -; mRNA.
DR   EMBL; BC101588; AAI01589.1; -; mRNA.
DR   CCDS; CCDS5869.1; -.
DR   RefSeq; NP_061853.1; NM_018980.2.
DR   AlphaFoldDB; Q9NYW4; -.
DR   SMR; Q9NYW4; -.
DR   BioGRID; 119946; 7.
DR   IntAct; Q9NYW4; 6.
DR   STRING; 9606.ENSP00000247883; -.
DR   ChEMBL; CHEMBL4523473; -.
DR   GuidetoPHARMACOLOGY; 662; -.
DR   GlyCosmos; Q9NYW4; 1 site, No reported glycans.
DR   GlyGen; Q9NYW4; 1 site.
DR   iPTMnet; Q9NYW4; -.
DR   PhosphoSitePlus; Q9NYW4; -.
DR   BioMuta; TAS2R5; -.
DR   DMDM; 29839664; -.
DR   PaxDb; 9606-ENSP00000247883; -.
DR   Antibodypedia; 32509; 99 antibodies from 17 providers.
DR   DNASU; 54429; -.
DR   Ensembl; ENST00000247883.5; ENSP00000247883.4; ENSG00000127366.5.
DR   GeneID; 54429; -.
DR   KEGG; hsa:54429; -.
DR   MANE-Select; ENST00000247883.5; ENSP00000247883.4; NM_018980.3; NP_061853.1.
DR   UCSC; uc003vwr.2; human.
DR   AGR; HGNC:14912; -.
DR   CTD; 54429; -.
DR   DisGeNET; 54429; -.
DR   GeneCards; TAS2R5; -.
DR   HGNC; HGNC:14912; TAS2R5.
DR   HPA; ENSG00000127366; Low tissue specificity.
DR   MIM; 605062; gene.
DR   neXtProt; NX_Q9NYW4; -.
DR   OpenTargets; ENSG00000127366; -.
DR   PharmGKB; PA37924; -.
DR   VEuPathDB; HostDB:ENSG00000127366; -.
DR   eggNOG; ENOG502S2SI; Eukaryota.
DR   GeneTree; ENSGT01100000263477; -.
DR   HOGENOM; CLU_072337_1_1_1; -.
DR   InParanoid; Q9NYW4; -.
DR   OMA; CRFLLQW; -.
DR   OrthoDB; 5263908at2759; -.
DR   PhylomeDB; Q9NYW4; -.
DR   TreeFam; TF335891; -.
DR   PathwayCommons; Q9NYW4; -.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR   Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR   SignaLink; Q9NYW4; -.
DR   BioGRID-ORCS; 54429; 9 hits in 1151 CRISPR screens.
DR   GeneWiki; TAS2R5; -.
DR   GenomeRNAi; 54429; -.
DR   Pharos; Q9NYW4; Tchem.
DR   PRO; PR:Q9NYW4; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q9NYW4; Protein.
DR   Bgee; ENSG00000127366; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 96 other cell types or tissues.
DR   ExpressionAtlas; Q9NYW4; baseline and differential.
DR   GO; GO:0016020; C:membrane; IC:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0008527; F:taste receptor activity; TAS:UniProtKB.
DR   GO; GO:0007635; P:chemosensory behavior; TAS:ProtInc.
DR   GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB.
DR   CDD; cd13950; 7tm_TAS2R; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR007960; TAS2R.
DR   PANTHER; PTHR11394; TASTE RECEPTOR TYPE 2; 1.
DR   PANTHER; PTHR11394:SF8; TASTE RECEPTOR TYPE 2 MEMBER 5; 1.
DR   Pfam; PF05296; TAS2R; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   Genevisible; Q9NYW4; HS.
PE   1: Evidence at protein level;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..299
FT                   /note="Taste receptor type 2 member 5"
FT                   /id="PRO_0000082212"
FT   TOPO_DOM        1
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        2..22
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..45
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        67..82
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        104..127
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..148
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        149..175
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        197..223
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        245..253
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        254..274
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        275..299
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        155
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         20
FT                   /note="G -> S (in dbSNP:rs2234013)"
FT                   /id="VAR_053343"
FT   VARIANT         26
FT                   /note="S -> I (in dbSNP:rs2227264)"
FT                   /id="VAR_020203"
FT   VARIANT         113
FT                   /note="P -> L (in dbSNP:rs2234014)"
FT                   /id="VAR_034537"
FT   VARIANT         167
FT                   /note="Y -> C (in dbSNP:rs34529840)"
FT                   /id="VAR_034538"
FT   VARIANT         213
FT                   /note="R -> Q (in dbSNP:rs2234015)"
FT                   /id="VAR_024184"
FT   VARIANT         294
FT                   /note="R -> L (in dbSNP:rs2234016)"
FT                   /id="VAR_053344"
SQ   SEQUENCE   299 AA;  34505 MW;  F85064A07E9018C7 CRC64;
     MLSAGLGLLM LVAVVEFLIG LIGNGSLVVW SFREWIRKFN WSSYNLIILG LAGCRFLLQW
     LIILDLSLFP LFQSSRWLRY LSIFWVLVSQ ASLWFATFLS VFYCKKITTF DRPAYLWLKQ
     RAYNLSLWCL LGYFIINLLL TVQIGLTFYH PPQGNSSIRY PFESWQYLYA FQLNSGSYLP
     LVVFLVSSGM LIVSLYTHHK KMKVHSAGRR DVRAKAHITA LKSLGCFLLL HLVYIMASPF
     SITSKTYPPD LTSVFIWETL MAAYPSLHSL ILIMGIPRVK QTCQKILWKT VCARRCWGP
//