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Q9NYW2 (TA2R8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Taste receptor type 2 member 8

Short name=T2R8
Alternative name(s):
Taste receptor family B member 5
Short name=TRB5
Gene names
Name:TAS2R8
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.

Miscellaneous

Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.

Sequence similarities

Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
   Biological processSensory transduction
Taste
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdetection of chemical stimulus involved in sensory perception of taste

Traceable author statement Ref.1. Source: GOC

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functiontaste receptor activity

Traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Taste receptor type 2 member 8
PRO_0000082227

Regions

Topological domain1 – 77Extracellular Potential
Transmembrane8 – 2821Helical; Name=1; Potential
Topological domain29 – 5022Cytoplasmic Potential
Transmembrane51 – 7121Helical; Name=2; Potential
Topological domain72 – 8211Extracellular Potential
Transmembrane83 – 10321Helical; Name=3; Potential
Topological domain104 – 13128Cytoplasmic Potential
Transmembrane132 – 15221Helical; Name=4; Potential
Topological domain153 – 18432Extracellular Potential
Transmembrane185 – 20521Helical; Name=5; Potential
Topological domain206 – 23934Cytoplasmic Potential
Transmembrane240 – 26021Helical; Name=6; Potential
Topological domain261 – 2666Extracellular Potential
Transmembrane267 – 28721Helical; Name=7; Potential
Topological domain288 – 30922Cytoplasmic Potential

Amino acid modifications

Glycosylation1671N-linked (GlcNAc...) Potential

Natural variations

Natural variant3081M → V. Ref.2
Corresponds to variant rs2537817 [ dbSNP | Ensembl ].
VAR_024186

Sequences

Sequence LengthMass (Da)Tools
Q9NYW2 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 5E3D94B726A52413

FASTA30935,877
        10         20         30         40         50         60 
MFSPADNIFI ILITGEFILG ILGNGYIALV NWIDWIKKKK ISTVDYILTN LVIARICLIS 

        70         80         90        100        110        120 
VMVVNGIVIV LNPDVYTKNK QQIVIFTFWT FANYLNMWIT TCLNVFYFLK IASSSHPLFL 

       130        140        150        160        170        180 
WLKWKIDMVV HWILLGCFAI SLLVSLIAAI VLSCDYRFHA IAKHKRNITE MFHVSKIPYF 

       190        200        210        220        230        240 
EPLTLFNLFA IVPFIVSLIS FFLLVRSLWR HTKQIKLYAT GSRDPSTEVH VRAIKTMTSF 

       250        260        270        280        290        300 
IFFFFLYYIS SILMTFSYLM TKYKLAVEFG EIAAILYPLG HSLILIVLNN KLRQTFVRML 


TCRKIACMI 

« Hide

References

« Hide 'large scale' references
[1]"A novel family of mammalian taste receptors."
Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., Zuker C.S.
Cell 100:693-702(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TOPOLOGY.
[2]"Evolution of bitter taste receptors in humans and apes."
Fischer A., Gilad Y., Man O., Paeaebo S.
Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-308.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"T2Rs function as bitter taste receptors."
Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., Zuker C.S., Ryba N.J.P.
Cell 100:703-711(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Receptors for bitter and sweet taste."
Montmayeur J.-P., Matsunami H.
Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[6]"Molecular mechanisms of bitter and sweet taste transduction."
Margolskee R.F.
J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[7]"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways."
Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J.
Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF227134 Genomic DNA. Translation: AAF43907.1.
AY724935 Genomic DNA. Translation: AAU21137.1.
BC069084 mRNA. Translation: AAH69084.1.
BC069551 mRNA. Translation: AAH69551.1.
BC096701 mRNA. Translation: AAH96701.1.
BC096735 mRNA. Translation: AAH96735.1.
BC098353 mRNA. Translation: AAH98353.1.
RefSeqNP_076407.1. NM_023918.1.
UniGeneHs.533755.

3D structure databases

ProteinModelPortalQ9NYW2.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9NYW2.

Polymorphism databases

DMDM29839662.

Proteomic databases

PaxDbQ9NYW2.
PRIDEQ9NYW2.

Protocols and materials databases

DNASU50836.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000240615; ENSP00000240615; ENSG00000121314.
ENST00000572823; ENSP00000461158; ENSG00000272712.
ENST00000573426; ENSP00000459504; ENSG00000273359.
GeneID50836.
KEGGhsa:50836.
UCSCuc010shh.2. human.

Organism-specific databases

CTD50836.
GeneCardsGC12M010858.
HGNCHGNC:14915. TAS2R8.
MIM604794. gene.
neXtProtNX_Q9NYW2.
PharmGKBPA37926.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG249394.
HOGENOMHOG000234595.
HOVERGENHBG060698.
InParanoidQ9NYW2.
KOK08474.
OMASPADNIF.
OrthoDBEOG72C512.
PhylomeDBQ9NYW2.
TreeFamTF335891.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

CleanExHS_TAS2R8.
GenevestigatorQ9NYW2.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR017452. GPCR_Rhodpsn_7TM.
IPR007960. TAS2_rcpt.
[Graphical view]
PfamPF05296. TAS2R. 1 hit.
[Graphical view]
PROSITEPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTAS2R8.
GenomeRNAi50836.
NextBio53293.
PROQ9NYW2.
SOURCESearch...

Entry information

Entry nameTA2R8_HUMAN
AccessionPrimary (citable) accession number: Q9NYW2
Secondary accession number(s): Q4KN29, Q645Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries