Q9NYW2 (TA2R8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taste receptor type 2 member 8
Short name=T2R8

Alternative name(s):
Taste receptor family B member 5
Short name=TRB5

Gene names

Name:

TAS2R8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.
Miscellaneous	Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.
Sequence similarities	Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Non-traceable author statement. Source: UniProtKB
Molecular_function	taste receptor activity Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Taste receptor type 2 member 8		PRO_0000082227
Regions
	Topological domain	1 – 7	7	Extracellular Potential
	Transmembrane	8 – 28	21	Helical; Name=1; Potential
	Topological domain	29 – 50	22	Cytoplasmic Potential
	Transmembrane	51 – 71	21	Helical; Name=2; Potential
	Topological domain	72 – 82	11	Extracellular Potential
	Transmembrane	83 – 103	21	Helical; Name=3; Potential
	Topological domain	104 – 131	28	Cytoplasmic Potential
	Transmembrane	132 – 152	21	Helical; Name=4; Potential
	Topological domain	153 – 184	32	Extracellular Potential
	Transmembrane	185 – 205	21	Helical; Name=5; Potential
	Topological domain	206 – 239	34	Cytoplasmic Potential
	Transmembrane	240 – 260	21	Helical; Name=6; Potential
	Topological domain	261 – 266	6	Extracellular Potential
	Transmembrane	267 – 287	21	Helical; Name=7; Potential
	Topological domain	288 – 309	22	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	308	1	M → V. Ref.2 Corresponds to variant rs2537817 [ dbSNP | Ensembl ].		VAR_024186

Sequences

	Sequence		Length	Mass (Da)
	Q9NYW2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5E3D94B726A52413	FASTA	309	35,877
	10 20 30 40 50 60 MFSPADNIFI ILITGEFILG ILGNGYIALV NWIDWIKKKK ISTVDYILTN LVIARICLIS 70 80 90 100 110 120 VMVVNGIVIV LNPDVYTKNK QQIVIFTFWT FANYLNMWIT TCLNVFYFLK IASSSHPLFL 130 140 150 160 170 180 WLKWKIDMVV HWILLGCFAI SLLVSLIAAI VLSCDYRFHA IAKHKRNITE MFHVSKIPYF 190 200 210 220 230 240 EPLTLFNLFA IVPFIVSLIS FFLLVRSLWR HTKQIKLYAT GSRDPSTEVH VRAIKTMTSF 250 260 270 280 290 300 IFFFFLYYIS SILMTFSYLM TKYKLAVEFG EIAAILYPLG HSLILIVLNN KLRQTFVRML TCRKIACMI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel family of mammalian taste receptors." Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., Zuker C.S. Cell 100:693-702(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TOPOLOGY.
[2]	"Evolution of bitter taste receptors in humans and apes." Fischer A., Gilad Y., Man O., Paeaebo S. Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-308.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"T2Rs function as bitter taste receptors." Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., Zuker C.S., Ryba N.J.P. Cell 100:703-711(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Receptors for bitter and sweet taste." Montmayeur J.-P., Matsunami H. Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[6]	"Molecular mechanisms of bitter and sweet taste transduction." Margolskee R.F. J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J. Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF227134 Genomic DNA. Translation: AAF43907.1. AY724935 Genomic DNA. Translation: AAU21137.1. BC069084 mRNA. Translation: AAH69084.1. BC069551 mRNA. Translation: AAH69551.1. BC096701 mRNA. Translation: AAH96701.1. BC096735 mRNA. Translation: AAH96735.1. BC098353 mRNA. Translation: AAH98353.1.
IPI	IPI00028325.
RefSeq	NP_076407.1. NM_023918.1.
UniGene	Hs.533755.
3D structure databases
ProteinModelPortal	Q9NYW2.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9NYW2.
Polymorphism databases
DMDM	29839662.
Proteomic databases
PaxDb	Q9NYW2.
PRIDE	Q9NYW2.
Protocols and materials databases
DNASU	50836.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000240615; ENSP00000240615; ENSG00000121314. ENST00000572823; ENSP00000461158; ENSG00000269828. ENST00000573426; ENSP00000459504; ENSG00000268701.
GeneID	50836.
KEGG	hsa:50836.
UCSC	uc010shh.2. human.
Organism-specific databases
CTD	50836.
GeneCards	GC12M010858.
HGNC	HGNC:14915. TAS2R8.
MIM	604794. gene.
neXtProt	NX_Q9NYW2.
PharmGKB	PA37926.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG249394.
HOGENOM	HOG000234595.
HOVERGEN	HBG060698.
InParanoid	Q9NYW2.
KO	K08474.
OMA	SPADNIF.
OrthoDB	EOG4NZTTR.
PhylomeDB	Q9NYW2.
Gene expression databases
ArrayExpress	Q9NYW2.
CleanEx	HS_TAS2R8.
Genevestigator	Q9NYW2.
GermOnline	ENSG00000121314. Homo sapiens.
Family and domain databases
InterPro	IPR017452. GPCR_Rhodpsn_7TM. IPR007960. TAS2_rcpt. [Graphical view]
Pfam	PF05296. TAS2R. 1 hit. [Graphical view]
PROSITE	PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	50836.
NextBio	53293.
SOURCE	Search...

Entry information

Entry name

TA2R8_HUMAN

Accession

Primary (citable) accession number: Q9NYW2
Secondary accession number(s): Q4KN29, Q645Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.