Q9NYV8 (T2R14_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taste receptor type 2 member 14
Short name=T2R14

Alternative name(s):
Taste receptor family B member 1
Short name=TRB1

Gene names

Name:

TAS2R14

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells.
Miscellaneous	Most taste cells may be activated by a limited number of bitter compounds; individual taste cells can discriminate among bitter stimuli.
Sequence similarities	Belongs to the G-protein coupled receptor T2R family.

Ontologies

Keywords
Biological process	Sensory transduction Taste
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred by curator PubMed 16720576. Source: HGNC
Molecular_function	bitter taste receptor activity Inferred from direct assay PubMed 16720576. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Taste receptor type 2 member 14		PRO_0000082255
Regions
	Topological domain	1 – 7	7	Extracellular Potential
	Transmembrane	8 – 28	21	Helical; Name=1; Potential
	Topological domain	29 – 55	27	Cytoplasmic Potential
	Transmembrane	56 – 76	21	Helical; Name=2; Potential
	Topological domain	77 – 87	11	Extracellular Potential
	Transmembrane	88 – 108	21	Helical; Name=3; Potential
	Topological domain	109 – 129	21	Cytoplasmic Potential
	Transmembrane	130 – 150	21	Helical; Name=4; Potential
	Topological domain	151 – 184	34	Extracellular Potential
	Transmembrane	185 – 205	21	Helical; Name=5; Potential
	Topological domain	206 – 232	27	Cytoplasmic Potential
	Transmembrane	233 – 253	21	Helical; Name=6; Potential
	Topological domain	254 – 261	8	Extracellular Potential
	Transmembrane	262 – 282	21	Helical; Name=7; Potential
	Topological domain	283 – 317	35	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	153	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	171	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	86	1	T → A. Corresponds to variant rs16925868 [ dbSNP | Ensembl ].		VAR_053347
	Natural variant	201	1	L → F. Corresponds to variant rs35804287 [ dbSNP | Ensembl ].		VAR_062085

Sequences

	Sequence		Length	Mass (Da)
	Q9NYV8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C79184343D679BE5	FASTA	317	36,160
	10 20 30 40 50 60 MGGVIKSIFT FVLIVEFIIG NLGNSFIALV NCIDWVKGRK ISSVDRILTA LAISRISLVW 70 80 90 100 110 120 LIFGSWCVSV FFPALFATEK MFRMLTNIWT VINHFSVWLA TGLGTFYFLK IANFSNSIFL 130 140 150 160 170 180 YLKWRVKKVV LVLLLVTSVF LFLNIALINI HINASINGYR RNKTCSSDSS NFTRFSSLIV 190 200 210 220 230 240 LTSTVFIFIP FTLSLAMFLL LIFSMWKHRK KMQHTVKISG DASTKAHRGV KSVITFFLLY 250 260 270 280 290 300 AIFSLSFFIS VWTSERLEEN LIILSQVMGM AYPSCHSCVL ILGNKKLRQA SLSVLLWLRY 310 MFKDGEPSGH KEFRESS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel family of mammalian taste receptors." Adler E., Hoon M.A., Mueller K.L., Chandrashekar J., Ryba N.J.P., Zuker C.S. Cell 100:693-702(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TOPOLOGY.
[2]	"Evolution of bitter taste receptors in humans and apes." Fischer A., Gilad Y., Man O., Paeaebo S. Mol. Biol. Evol. 22:432-436(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"T2Rs function as bitter taste receptors." Chandrashekar J., Mueller K.L., Hoon M.A., Adler E., Feng L., Guo W., Zuker C.S., Ryba N.J.P. Cell 100:703-711(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Receptors for bitter and sweet taste." Montmayeur J.-P., Matsunami H. Curr. Opin. Neurobiol. 12:366-371(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[6]	"Molecular mechanisms of bitter and sweet taste transduction." Margolskee R.F. J. Biol. Chem. 277:1-4(2002) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[7]	"Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways." Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D., Zuker C.S., Ryba N.J. Cell 112:293-301(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[8]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-153, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF227138 Genomic DNA. Translation: AAF43911.1. AY724944 Genomic DNA. Translation: AAU21146.1. BC069148 mRNA. Translation: AAH69148.1. BC101993 mRNA. Translation: AAI01994.1. BC101994 mRNA. Translation: AAI01995.1. BC103699 mRNA. Translation: AAI03700.1.
IPI	IPI00028350.
RefSeq	NP_076411.1. NM_023922.1.
UniGene	Hs.679406. Hs.731575.
3D structure databases
ProteinModelPortal	Q9NYV8.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000375094.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9NYV8.
Polymorphism databases
DMDM	29839658.
Proteomic databases
PaxDb	Q9NYV8.
PRIDE	Q9NYV8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000537503; ENSP00000441949; ENSG00000212127. ENST00000574901; ENSP00000460620; ENSG00000262103. ENST00000575008; ENSP00000458544; ENSG00000261984.
GeneID	50840.
KEGG	hsa:50840.
UCSC	uc010shi.2. human.
Organism-specific databases
CTD	50840.
GeneCards	GC12M011090.
HGNC	HGNC:14920. TAS2R14.
MIM	604790. gene.
neXtProt	NX_Q9NYV8.
PharmGKB	PA37930.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46292.
HOGENOM	HOG000234595.
HOVERGEN	HBG060698.
InParanoid	Q9NYV8.
KO	K08474.
OMA	LINIHIN.
OrthoDB	EOG42822D.
PhylomeDB	Q9NYV8.
Gene expression databases
ArrayExpress	Q9NYV8.
Bgee	Q9NYV8.
CleanEx	HS_TAS2R14.
Genevestigator	Q9NYV8.
Family and domain databases
InterPro	IPR007960. TAS2_rcpt. [Graphical view]
Pfam	PF05296. TAS2R. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	50840.
NextBio	53309.
SOURCE	Search...

Entry information

Entry name

T2R14_HUMAN

Accession

Primary (citable) accession number: Q9NYV8
Secondary accession number(s): Q645X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.