Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA polymerase I-specific transcription initiation factor RRN3

Gene

RRN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity).By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
SIGNORiQ9NYV6

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase I-specific transcription initiation factor RRN3
Alternative name(s):
Transcription initiation factor IA
Short name:
TIF-IA
Gene namesi
Name:RRN3
Synonyms:TIFIA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000085721.12
HGNCiHGNC:30346 RRN3
MIMi605121 gene
neXtProtiNX_Q9NYV6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi411 – 415Missing : Abolishes interaction with TAF1B and TAF1C. 1 Publication5

Organism-specific databases

DisGeNETi54700
OpenTargetsiENSG00000085721
PharmGKBiPA134878601

Polymorphism and mutation databases

BioMutaiRRN3
DMDMi74719591

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002114261 – 651RNA polymerase I-specific transcription initiation factor RRN3Add BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170PhosphoserineCombined sources1
Modified residuei172PhosphoserineCombined sources1
Modified residuei200Phosphothreonine; by MAPK91 Publication1
Modified residuei640PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation is required for participation in rDNA transcription (By similarity). Phosphorylated at Thr-200 by MAPK9/JNK2, which abrogates initiation complex formation.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NYV6
MaxQBiQ9NYV6
PaxDbiQ9NYV6
PeptideAtlasiQ9NYV6
PRIDEiQ9NYV6
ProteomicsDBi83282

PTM databases

iPTMnetiQ9NYV6
PhosphoSitePlusiQ9NYV6

Expressioni

Gene expression databases

BgeeiENSG00000085721
CleanExiHS_RRN3
ExpressionAtlasiQ9NYV6 baseline and differential
GenevisibleiQ9NYV6 HS

Organism-specific databases

HPAiHPA049837

Interactioni

Subunit structurei

Interacts with TWISTNB, EIF3L, TAF1B and TAF1C.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi120102, 31 interactors
CORUMiQ9NYV6
IntActiQ9NYV6, 4 interactors
MINTiQ9NYV6
STRINGi9606.ENSP00000198767

Structurei

3D structure databases

ProteinModelPortaliQ9NYV6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni500 – 651Interaction with TWISTNB1 PublicationAdd BLAST152
Regioni557 – 651Interaction with EIF3L1 PublicationAdd BLAST95

Sequence similaritiesi

Belongs to the RRN3 family.Curated

Phylogenomic databases

eggNOGiKOG2434 Eukaryota
ENOG410XRJJ LUCA
GeneTreeiENSGT00390000001488
HOGENOMiHOG000132846
HOVERGENiHBG056994
InParanoidiQ9NYV6
KOiK15216
OMAiPLKVCSP
OrthoDBiEOG091G089T
PhylomeDBiQ9NYV6
TreeFamiTF312979

Family and domain databases

InterProiView protein in InterPro
IPR007991 RNA_pol_I_trans_ini_fac_RRN3
PANTHERiPTHR12790 PTHR12790, 1 hit
PfamiView protein in Pfam
PF05327 RRN3, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NYV6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPLLHTRL PGDAAASSSA VKKLGASRTG ISNMRALEND FFNSPPRKTV
60 70 80 90 100
RFGGTVTEVL LKYKKGETND FELLKNQLLD PDIKDDQIIN WLLEFRSSIM
110 120 130 140 150
YLTKDFEQLI SIILRLPWLN RSQTVVEEYL AFLGNLVSAQ TVFLRPCLSM
160 170 180 190 200
IASHFVPPRV IIKEGDVDVS DSDDEDDNLP ANFDTCHRAL QIIARYVPST
210 220 230 240 250
PWFLMPILVE KFPFVRKSER TLECYVHNLL RISVYFPTLR HEILELIIEK
260 270 280 290 300
LLKLDVNASR QGIEDAEETA TQTCGGTDST EGLFNMDEDE ETEHETKAGP
310 320 330 340 350
ERLDQMVHPV AERLDILMSL VLSYMKDVCY VDGKVDNGKT KDLYRDLINI
360 370 380 390 400
FDKLLLPTHA SCHVQFFMFY LCSFKLGFAE AFLEHLWKKL QDPSNPAIIR
410 420 430 440 450
QAAGNYIGSF LARAKFIPLI TVKSCLDLLV NWLHIYLNNQ DSGTKAFCDV
460 470 480 490 500
ALHGPFYSAC QAVFYTFVFR HKQLLSGNLK EGLQYLQSLN FERIVMSQLN
510 520 530 540 550
PLKICLPSVV NFFAAITNKY QLVFCYTIIE RNNRQMLPVI RSTAGGDSVQ
560 570 580 590 600
ICTNPLDTFF PFDPCVLKRS KKFIDPIYQV WEDMSAEELQ EFKKPMKKDI
610 620 630 640 650
VEDEDDDFLK GEVPQNDTVI GITPSSFDTH FRSPSSSVGS PPVLYMQPSP

L
Length:651
Mass (Da):74,107
Last modified:October 1, 2000 - v1
Checksum:iC646DC6677C49895
GO
Isoform 2 (identifier: Q9NYV6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     287-305: DEDEETEHETKAGPERLDQ → VSSLLMKVEMKFIIKGGNS
     306-651: Missing.

Note: No experimental confirmation available.
Show »
Length:305
Mass (Da):34,472
Checksum:i1745373E5CC8CE7B
GO
Isoform 3 (identifier: Q9NYV6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Note: No experimental confirmation available.
Show »
Length:618
Mass (Da):70,876
Checksum:i1D119D4C3DADB659
GO
Isoform 4 (identifier: Q9NYV6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.
     223-255: Missing.

Note: No experimental confirmation available.
Show »
Length:469
Mass (Da):53,315
Checksum:iFCAE200CD69B3663
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051886348I → M. Corresponds to variant dbSNP:rs2941256Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0565191 – 149Missing in isoform 4. 1 PublicationAdd BLAST149
Alternative sequenceiVSP_0565201 – 33Missing in isoform 3. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_056521223 – 255Missing in isoform 4. 1 PublicationAdd BLAST33
Alternative sequenceiVSP_056338287 – 305DEDEE…ERLDQ → VSSLLMKVEMKFIIKGGNS in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_056339306 – 651Missing in isoform 2. 1 PublicationAdd BLAST346

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272050 mRNA Translation: CAC07955.1
AF227156 mRNA Translation: AAF66160.1
AK303405 mRNA Translation: BAG64459.1
AK304856 mRNA Translation: BAG65594.1
AK314769 mRNA Translation: BAG37307.1
AC139256 Genomic DNA No translation available.
CH471301 Genomic DNA Translation: EAW54754.1
BC071868 mRNA Translation: AAH71868.1
BC104660 mRNA Translation: AAI04661.1
BC132688 mRNA Translation: AAI32689.1
BC132690 mRNA Translation: AAI32691.1
CCDSiCCDS10559.1 [Q9NYV6-1]
RefSeqiNP_060897.3, NM_018427.4 [Q9NYV6-1]
UniGeneiHs.460078
Hs.720348

Genome annotation databases

EnsembliENST00000198767; ENSP00000198767; ENSG00000085721 [Q9NYV6-1]
ENST00000327307; ENSP00000318484; ENSG00000085721 [Q9NYV6-3]
ENST00000564131; ENSP00000454238; ENSG00000085721 [Q9NYV6-2]
ENST00000616334; ENSP00000480743; ENSG00000278494 [Q9NYV6-1]
ENST00000632541; ENSP00000488651; ENSG00000278494 [Q9NYV6-3]
ENST00000634151; ENSP00000487821; ENSG00000278494 [Q9NYV6-2]
GeneIDi54700
KEGGihsa:54700
UCSCiuc002dde.4 human [Q9NYV6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRRN3_HUMAN
AccessioniPrimary (citable) accession number: Q9NYV6
Secondary accession number(s): A2RTY9
, B4E0J7, B4E3T2, Q3MHU9, Q6IPL4, Q9H4F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health