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Q9NYV6

- RRN3_HUMAN

UniProt

Q9NYV6 - RRN3_HUMAN

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Protein
RNA polymerase I-specific transcription initiation factor RRN3
Gene
RRN3, TIFIA
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction By similarity.4 Publications

GO - Biological processi

  1. cell proliferation Source: Ensembl
  2. cytoplasm organization Source: Ensembl
  3. gene expression Source: Reactome
  4. homeostasis of number of cells Source: Ensembl
  5. in utero embryonic development Source: Ensembl
  6. negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
  7. nucleolus organization Source: Ensembl
  8. positive regulation of transcription, DNA-templated Source: Ensembl
  9. regulation of DNA-templated transcription, initiation Source: Ensembl
  10. ribosome biogenesis Source: Ensembl
  11. transcription from RNA polymerase I promoter Source: Reactome
  12. transcription initiation from RNA polymerase I promoter Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_1913. RNA Polymerase I Promoter Escape.
REACT_953. RNA Polymerase I Transcription Initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase I-specific transcription initiation factor RRN3
Alternative name(s):
Transcription initiation factor IA
Short name:
TIF-IA
Gene namesi
Name:RRN3
Synonyms:TIFIA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:30346. RRN3.

Subcellular locationi

Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. nucleolus Source: HPA
  2. nucleoplasm Source: Reactome
  3. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi411 – 4155Missing: Abolishes interaction with TAF1B and TAF1C. 1 Publication

Organism-specific databases

PharmGKBiPA134878601.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 651651RNA polymerase I-specific transcription initiation factor RRN3
PRO_0000211426Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei170 – 1701Phosphoserine1 Publication
Modified residuei172 – 1721Phosphoserine1 Publication
Modified residuei200 – 2001Phosphothreonine; by MAPK91 Publication
Modified residuei640 – 6401Phosphoserine2 Publications

Post-translational modificationi

Phosphorylation is required for participation in rDNA transcription By similarity. Phosphorylated at Thr-200 by MAPK9/JNK2, which abrogates initiation complex formation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9NYV6.
PaxDbiQ9NYV6.
PRIDEiQ9NYV6.

PTM databases

PhosphoSiteiQ9NYV6.

Expressioni

Gene expression databases

ArrayExpressiQ9NYV6.
BgeeiQ9NYV6.
CleanExiHS_RRN3.
GenevestigatoriQ9NYV6.

Organism-specific databases

HPAiHPA049837.

Interactioni

Subunit structurei

Interacts with TWISTNB, EIF3L, TAF1B and TAF1C.2 Publications

Protein-protein interaction databases

BioGridi120102. 23 interactions.
IntActiQ9NYV6. 4 interactions.
MINTiMINT-249163.
STRINGi9606.ENSP00000198767.

Structurei

3D structure databases

ProteinModelPortaliQ9NYV6.
SMRiQ9NYV6. Positions 179-585.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni500 – 651152Interaction with TWISTNB
Add
BLAST
Regioni557 – 65195Interaction with EIF3L
Add
BLAST

Sequence similaritiesi

Belongs to the RRN3 family.

Phylogenomic databases

eggNOGiNOG275643.
HOGENOMiHOG000236344.
HOVERGENiHBG056994.
InParanoidiQ9NYV6.
KOiK15216.
OMAiFRWRDLT.
OrthoDBiEOG7ZGX2R.
PhylomeDBiQ9NYV6.
TreeFamiTF312979.

Family and domain databases

InterProiIPR007991. RNA_pol_I_trans_ini_fac_RRN3.
[Graphical view]
PANTHERiPTHR12790. PTHR12790. 1 hit.
PfamiPF05327. RRN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NYV6-1 [UniParc]FASTAAdd to Basket

« Hide

MAAPLLHTRL PGDAAASSSA VKKLGASRTG ISNMRALEND FFNSPPRKTV    50
RFGGTVTEVL LKYKKGETND FELLKNQLLD PDIKDDQIIN WLLEFRSSIM 100
YLTKDFEQLI SIILRLPWLN RSQTVVEEYL AFLGNLVSAQ TVFLRPCLSM 150
IASHFVPPRV IIKEGDVDVS DSDDEDDNLP ANFDTCHRAL QIIARYVPST 200
PWFLMPILVE KFPFVRKSER TLECYVHNLL RISVYFPTLR HEILELIIEK 250
LLKLDVNASR QGIEDAEETA TQTCGGTDST EGLFNMDEDE ETEHETKAGP 300
ERLDQMVHPV AERLDILMSL VLSYMKDVCY VDGKVDNGKT KDLYRDLINI 350
FDKLLLPTHA SCHVQFFMFY LCSFKLGFAE AFLEHLWKKL QDPSNPAIIR 400
QAAGNYIGSF LARAKFIPLI TVKSCLDLLV NWLHIYLNNQ DSGTKAFCDV 450
ALHGPFYSAC QAVFYTFVFR HKQLLSGNLK EGLQYLQSLN FERIVMSQLN 500
PLKICLPSVV NFFAAITNKY QLVFCYTIIE RNNRQMLPVI RSTAGGDSVQ 550
ICTNPLDTFF PFDPCVLKRS KKFIDPIYQV WEDMSAEELQ EFKKPMKKDI 600
VEDEDDDFLK GEVPQNDTVI GITPSSFDTH FRSPSSSVGS PPVLYMQPSP 650
L 651
Length:651
Mass (Da):74,107
Last modified:October 1, 2000 - v1
Checksum:iC646DC6677C49895
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti348 – 3481I → M.
Corresponds to variant rs2941256 [ dbSNP | Ensembl ].
VAR_051886

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ272050 mRNA. Translation: CAC07955.1.
AF227156 mRNA. Translation: AAF66160.1.
AK314769 mRNA. Translation: BAG37307.1.
CH471301 Genomic DNA. Translation: EAW54754.1.
BC071868 mRNA. Translation: AAH71868.1.
BC132688 mRNA. Translation: AAI32689.1.
BC132690 mRNA. Translation: AAI32691.1.
CCDSiCCDS10559.1.
RefSeqiNP_060897.3. NM_018427.3.
UniGeneiHs.460078.

Genome annotation databases

EnsembliENST00000198767; ENSP00000198767; ENSG00000085721.
GeneIDi54700.
KEGGihsa:54700.
UCSCiuc002dde.3. human.

Polymorphism databases

DMDMi74719591.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ272050 mRNA. Translation: CAC07955.1 .
AF227156 mRNA. Translation: AAF66160.1 .
AK314769 mRNA. Translation: BAG37307.1 .
CH471301 Genomic DNA. Translation: EAW54754.1 .
BC071868 mRNA. Translation: AAH71868.1 .
BC132688 mRNA. Translation: AAI32689.1 .
BC132690 mRNA. Translation: AAI32691.1 .
CCDSi CCDS10559.1.
RefSeqi NP_060897.3. NM_018427.3.
UniGenei Hs.460078.

3D structure databases

ProteinModelPortali Q9NYV6.
SMRi Q9NYV6. Positions 179-585.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120102. 23 interactions.
IntActi Q9NYV6. 4 interactions.
MINTi MINT-249163.
STRINGi 9606.ENSP00000198767.

PTM databases

PhosphoSitei Q9NYV6.

Polymorphism databases

DMDMi 74719591.

Proteomic databases

MaxQBi Q9NYV6.
PaxDbi Q9NYV6.
PRIDEi Q9NYV6.

Protocols and materials databases

DNASUi 54700.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000198767 ; ENSP00000198767 ; ENSG00000085721 .
GeneIDi 54700.
KEGGi hsa:54700.
UCSCi uc002dde.3. human.

Organism-specific databases

CTDi 54700.
GeneCardsi GC16M015153.
H-InvDB HIX0012886.
HIX0038660.
HIX0038739.
HGNCi HGNC:30346. RRN3.
HPAi HPA049837.
MIMi 605121. gene.
neXtProti NX_Q9NYV6.
PharmGKBi PA134878601.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG275643.
HOGENOMi HOG000236344.
HOVERGENi HBG056994.
InParanoidi Q9NYV6.
KOi K15216.
OMAi FRWRDLT.
OrthoDBi EOG7ZGX2R.
PhylomeDBi Q9NYV6.
TreeFami TF312979.

Enzyme and pathway databases

Reactomei REACT_1913. RNA Polymerase I Promoter Escape.
REACT_953. RNA Polymerase I Transcription Initiation.

Miscellaneous databases

GeneWikii RRN3.
GenomeRNAii 54700.
NextBioi 57257.
PROi Q9NYV6.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9NYV6.
Bgeei Q9NYV6.
CleanExi HS_RRN3.
Genevestigatori Q9NYV6.

Family and domain databases

InterProi IPR007991. RNA_pol_I_trans_ini_fac_RRN3.
[Graphical view ]
PANTHERi PTHR12790. PTHR12790. 1 hit.
Pfami PF05327. RRN3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "TIF-IA, the factor mediating growth-dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p."
    Bodem J., Dobreva G., Hoffmann-Rohrer U., Iben S., Zentgraf H., Delius H., Vingron M., Grummt I.
    EMBO Rep. 1:171-175(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human."
    Moorefield B., Greene E.A., Reeder R.H.
    Proc. Natl. Acad. Sci. U.S.A. 97:4724-4729(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Hippocampus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  6. "hRRN3 is essential in the SL1-mediated recruitment of RNA polymerase I to rRNA gene promoters."
    Miller G., Panov K.I., Friedrich J.K., Trinkle-Mulcahy L., Lamond A.I., Zomerdijk J.C.B.M.
    EMBO J. 20:1373-1382(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TAF1B AND TAF1C.
  7. "Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter."
    Yuan X., Zhao J., Zentgraf H., Hoffmann-Rohrer U., Grummt I.
    EMBO Rep. 3:1082-1087(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TWISTNB; EIF3L; TAF1B AND TAF1C, MUTAGENESIS OF 411-LEU--LYS-415.
  8. "The nucleolus as a stress sensor: JNK2 inactivates the transcription factor TIF-IA and down-regulates rRNA synthesis."
    Mayer C., Bierhoff H., Grummt I.
    Genes Dev. 19:933-941(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-200 BY MAPK9/JNK2, FUNCTION.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170; SER-172 AND SER-640, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-640, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  12. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRRN3_HUMAN
AccessioniPrimary (citable) accession number: Q9NYV6
Secondary accession number(s): A2RTY9, Q6IPL4, Q9H4F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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