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Reviewed, UniProtKB/Swiss-Prot Q9NYV4 (CD2L7_HUMAN)

Last modified July 7, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cell division cycle 2-related protein kinase 7
      Short name=CDC2-related protein kinase 7
    EC=2.7.11.22
Alternative name(s):
    Cdc2-related kinase, arginine/serine-rich
      Short name=CrkRS
Gene names
Name: CRKRS
Synonyms: CRK7, KIAA0904
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length1490 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in RNA splicing. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with CCNL1 and CCNL2 By similarity.

Subcellular location

Nucleus. Nucleus speckle. Note: Colocalized with nuclear speckles throughout interphase. Ref.1

Tissue specificity

Ubiquitously expressed. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9NYV4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9NYV4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1254-1262: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14901490Cell division cycle 2-related protein kinase 7
PRO_0000085715

Regions

Domain727 – 1020294Protein kinase
Nucleotide binding733 – 7419ATP By similarity
Compositional bias407 – 4137Poly-Ala
Compositional bias535 – 5406Poly-Pro
Compositional bias1266 – 128015Poly-Pro

Sites

Active site8591Proton acceptor By similarity
Binding site7561ATP By similarity

Amino acid modifications

Modified residue241Phosphoserine Ref.9
Modified residue251Phosphoserine Ref.9
Modified residue321Phosphoserine Ref.9
Modified residue571Phosphothreonine Ref.9
Modified residue731Phosphotyrosine Ref.9
Modified residue771Phosphoserine Ref.9 Ref.6
Modified residue781Phosphoserine Ref.9 Ref.6
Modified residue801Phosphoserine Ref.9 Ref.6
Modified residue841Phosphoserine Ref.9 Ref.6
Modified residue2151Phosphoserine Ref.5
Modified residue2361Phosphoserine Ref.9
Modified residue2381Phosphoserine Ref.9
Modified residue2491Phosphoserine Ref.9 Ref.5 Ref.10
Modified residue2651Phosphoserine Ref.5
Modified residue2741Phosphoserine Ref.9 Ref.6 Ref.8
Modified residue2761Phosphoserine Ref.9 Ref.6 Ref.8
Modified residue2791Phosphotyrosine By similarity
Modified residue2911Phosphoserine Ref.9
Modified residue2931Phosphoserine Ref.9 Ref.5
Modified residue3011Phosphoserine Ref.9 Ref.10
Modified residue3031Phosphoserine Ref.9 Ref.5 Ref.10
Modified residue3181Phosphoserine Ref.10
Modified residue3191Phosphotyrosine By similarity
Modified residue3201Phosphoserine Ref.10
Modified residue3231Phosphoserine Ref.10
Modified residue3251Phosphoserine Ref.10
Modified residue3321Phosphoserine Ref.10
Modified residue3331Phosphoserine Ref.10
Modified residue3341Phosphoserine Ref.10
Modified residue3411Phosphoserine Ref.9
Modified residue3431Phosphoserine Ref.9 Ref.10
Modified residue3451Phosphoserine Ref.10
Modified residue3821Phosphoserine Ref.10
Modified residue3831Phosphoserine Ref.9 Ref.10 Ref.8
Modified residue3851Phosphoserine Ref.9 Ref.10 Ref.8
Modified residue4001Phosphoserine Ref.9 Ref.10
Modified residue4231Phosphoserine Ref.9 Ref.6 Ref.5 Ref.10 Ref.8 Ref.7
Modified residue5141Phosphothreonine Ref.9
Modified residue6811Phosphoserine Ref.9 Ref.6 Ref.10 Ref.8
Modified residue6851Phosphoserine Ref.9 Ref.6 Ref.10 Ref.8
Modified residue6921Phosphothreonine Ref.9 Ref.5 Ref.10 Ref.7
Modified residue8931Phosphothreonine Ref.9 Ref.8
Modified residue10831Phosphoserine Ref.9 Ref.5
Modified residue12441Phosphothreonine Ref.9

Natural variations

Alternative sequence1254 – 12629Missing in isoform 2.
VSP_030284
Natural variant5301P → A Ref.12
VAR_041968
Natural variant9121R → H in a colorectal adenocarcinoma sample; somatic mutation. Ref.12
VAR_041969
Natural variant11891L → Q Ref.12
VAR_041970
Natural variant12751P → L Ref.12
VAR_041971

Experimental info

Sequence conflict6391G → D in BAA74927. Ref.2
Sequence conflict6391G → D in AAI50266. Ref.4
Sequence conflict7451R → K in BAA74927. Ref.2
Sequence conflict7451R → K in AAI50266. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 851E18DF3BD2B1A1

FASTA1,490164,155
        10         20         30         40         50         60 
MPNSERHGGK KDGSGGASGT LQPSSGGGSS NSRERHRLVS KHKRHKSKHS KDMGLVTPEA 

        70         80         90        100        110        120 
ASLGTVIKPL VEYDDISSDS DTFSDDMAFK LDRRENDERR GSDRSDRLHK HRHHQHRRSR 

       130        140        150        160        170        180 
DLLKAKQTEK EKSQEVSSKS GSMKDRISGS SKRSNEETDD YGKAQVAKSS SKESRSSKLH 

       190        200        210        220        230        240 
KEKTRKEREL KSGHKDRSKS HRKRETPKSY KTVDSPKRRS RSPHRKWSDS SKQDDSPSGA 

       250        260        270        280        290        300 
SYGQDYDLSP SRSHTSSNYD SYKKSPGSTS RRQSVSPPYK EPSAYQSSTR SPSPYSRRQR 

       310        320        330        340        350        360 
SVSPYSRRRS SSYERSGSYS GRSPSPYGRR RSSSPFLSKR SLSRSPLPSR KSMKSRSRSP 

       370        380        390        400        410        420 
AYSRHSSSHS KKKRSSSRSR HSSISPVRLP LNSSLGAELS RKKKERAAAA AAAKMDGKES 

       430        440        450        460        470        480 
KGSPVFLPRK ENSSVEAKDS GLESKKLPRS VKLEKSAPDT ELVNVTHLNT EVKNSSDTGK 

       490        500        510        520        530        540 
VKLDENSEKH LVKDLKAQGT RDSKPIALKE EIVTPKETET SEKETPPPLP TIASPPPPLP 

       550        560        570        580        590        600 
TTTPPPQTPP LPPLPPIPAL PQQPPLPPSQ PAFSQVPASS TSTLPPSTHS KTSAVSSQAN 

       610        620        630        640        650        660 
SQPPVQVSVK TQVSVTAAIP HLKTSTLPPL PLPPLLPGGD DMDSPKETLP SKPVKKEKEQ 

       670        680        690        700        710        720 
RTRHLLTDLP LPPELPGGDL SPPDSPEPKA ITPPQQPYKK RPKICCPRYG ERRQTESDWG 

       730        740        750        760        770        780 
KRCVDKFDII GIIGEGTYGQ VYKARDKDTG ELVALKKVRL DNEKEGFPIT AIREIKILRQ 

       790        800        810        820        830        840 
LIHRSVVNMK EIVTDKQDAL DFKKDKGAFY LVFEYMDHDL MGLLESGLVH FSEDHIKSFM 

       850        860        870        880        890        900 
KQLMEGLEYC HKKNFLHRDI KCSNILLNNS GQIKLADFGL ARLYNSEESR PYTNKVITLW 

       910        920        930        940        950        960 
YRPPELLLGE ERYTPAIDVW SCGCILGELF TKKPIFQANL ELAQLELISR LCGSPCPAVW 

       970        980        990       1000       1010       1020 
PDVIKLPYFN TMKPKKQYRR RLREEFSFIP SAALDLLDHM LTLDPSKRCT AEQTLQSDFL 

      1030       1040       1050       1060       1070       1080 
KDVELSKMAP PDLPHWQDCH ELWSKKRRRQ RQSGVVVEEP PPSKTSRKET TSGTSTEPVK 

      1090       1100       1110       1120       1130       1140 
NSSPAPPQPA PGKVESGAGD AIGLADITQQ LNQSELAVLL NLLQSQTDLS IPQMAQLLNI 

      1150       1160       1170       1180       1190       1200 
HSNPEMQQQL EALNQSISAL TEATSQQQDS ETMAPEESLK EAPSAPVILP SAEQMTLEAS 

      1210       1220       1230       1240       1250       1260 
STPADMQNIL AVLLSQLMKT QEPAGSLEEN NSDKNSGPQG PRRTPTMPQE EAAACPPHIL 

      1270       1280       1290       1300       1310       1320 
PPEKRPPEPP GPPPPPPPPP LVEGDLSSAP QELNPAVTAA LLQLLSQPEA EPPGHLPHEH 

      1330       1340       1350       1360       1370       1380 
QALRPMEYST RPRPNRTYGN TDGPETGFSA IDTDERNSGP ALTESLVQTL VKNRTFSGSL 

      1390       1400       1410       1420       1430       1440 
SHLGESSSYQ GTGSVQFPGD QDLRFARVPL ALHPVVGQPF LKAEGSSNSV VHAETKLQNY 

      1450       1460       1470       1480       1490 
GELGPGTTGA SSSGAGLHWG GPTQSSAYGK LYRGPTRVPP RGGRGRGVPY

« Hide

Isoform 2.

Checksum: 3E98226CCF138F20
Show »

FASTA1,481163,229

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References

« Hide 'large scale' references
[1]"CrkRS: a novel conserved Cdc2-related protein kinase that colocalises with SC35 speckles."
Ko T.K., Kelly E., Pines J.
J. Cell Sci. 114:2591-2603(2001) [PubMed: 11683387] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
[2]"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:355-364(1998) [PubMed: 10048485] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed: 12168954] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]"Large-scale characterization of HeLa cell nuclear phosphoproteins."
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215; SER-249; SER-265; SER-293; SER-303; SER-423; THR-692 AND SER-1083, MASS SPECTROMETRY.
Tissue: Epithelium.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-78; SER-80; SER-84; SER-274; SER-276; SER-423; SER-681 AND SER-685, MASS SPECTROMETRY.
Tissue: Epithelium.
[7]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-423 AND THR-692, MASS SPECTROMETRY.
Tissue: Epithelium.
[8]"Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry."
Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R., Keri G., Wehland J., Daub H.
Mol. Cell. Proteomics 6:537-547(2007) [PubMed: 17192257] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-274; SER-276; SER-383; SER-385; SER-423; SER-681; SER-685 AND THR-893, MASS SPECTROMETRY.
[9]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-25; SER-32; THR-57; TYR-73; SER-77; SER-78; SER-80; SER-84; SER-236; SER-238; SER-249; SER-274; SER-276; SER-291; SER-293; SER-301; SER-303; SER-341; SER-343; SER-383; SER-385; SER-400; SER-423; THR-514; SER-681; SER-685; THR-692; THR-893; SER-1083 AND THR-1244, MASS SPECTROMETRY.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-249; SER-301; SER-303; SER-318; SER-320; SER-323; SER-325; SER-332; SER-333; SER-334; SER-343; SER-345; SER-382; SER-383; SER-385; SER-400; SER-423; SER-681; SER-685 AND THR-692, MASS SPECTROMETRY.
[11]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[12]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed: 17344846] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ALA-530; HIS-912; GLN-1189 AND LEU-1275.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF227198 mRNA. Translation: AAF36401.1.
AB020711 mRNA. Translation: BAA74927.2. Different initiation.
BC150265 mRNA. Translation: AAI50266.1.
IPIIPI00021175.
IPI00868781.
RefSeqNP_055898.1.
NP_057591.2.
UniGeneHs.416108

3D structure databases

HSSPHSSP built from PDB template 1P2A based on UniProtKB P24941.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9NYV4. 6 interactions.

PTM databases

PhosphoSiteQ9NYV4.

Proteomic databases

PRIDEQ9NYV4.

Genome annotation databases

EnsemblENSG00000167258. Homo sapiens. [Contig view]
GeneID51755.
KEGGhsa:51755.
UCSCuc002hrw.2. human.
uc010cvv.1. human.

Organism-specific databases

GeneCardsGC17P034872.
H-InvDBHIX0013777.
HGNCHGNC:24224. CRKRS.
HPAHPA008038.
PharmGKBPA142672078.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ9NYV4.
HOVERGENQ9NYV4.

Enzyme and pathway databases

BRENDA2.7.11.22. 247.

Gene expression databases

ArrayExpressQ9NYV4.
BgeeQ9NYV4.
CleanExHS_CRKRS.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio55858.

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Entry information

Entry nameCD2L7_HUMAN
AccessionPrimary (citable) accession number: Q9NYV4
Secondary accession number(s): A7E2B2, O94978
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: July 7, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents