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Q9NYU1

- UGGG2_HUMAN

UniProt

Q9NYU1 - UGGG2_HUMAN

Protein

UDP-glucose:glycoprotein glucosyltransferase 2

Gene

UGGT2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 4 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation By similarity.By similarity

    Cofactori

    Calcium or manganese.

    Pathwayi

    GO - Molecular functioni

    1. UDP-glucose:glycoprotein glucosyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. cellular protein metabolic process Source: Reactome
    2. post-translational protein modification Source: Reactome
    3. protein folding Source: Reactome
    4. protein N-linked glycosylation via asparagine Source: Reactome
    5. UDP-glucosylation Source: GOC

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_25091. ER Quality Control Compartment (ERQC).
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT24. Glycosyltransferase Family 24.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose:glycoprotein glucosyltransferase 2 (EC:2.4.1.-)
    Short name:
    UGT2
    Short name:
    hUGT2
    Alternative name(s):
    UDP--Glc:glycoprotein glucosyltransferase 2
    UDP-glucose ceramide glucosyltransferase-like 1
    Gene namesi
    Name:UGGT2
    Synonyms:UGCGL2, UGT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 13

    Organism-specific databases

    HGNCiHGNC:15664. UGGT2.

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation. Endoplasmic reticulum-Golgi intermediate compartment 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB-SubCell
    2. endoplasmic reticulum lumen Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA38015.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 15161489UDP-glucose:glycoprotein glucosyltransferase 2PRO_0000012274Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi286 – 2861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi920 – 9201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi950 – 9501N-linked (GlcNAc...)Sequence Analysis
    Modified residuei1289 – 12891Phosphotyrosine1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ9NYU1.
    PaxDbiQ9NYU1.
    PRIDEiQ9NYU1.

    PTM databases

    PhosphoSiteiQ9NYU1.

    Expressioni

    Tissue specificityi

    Higher levels in kidney, pancreas, heart, and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiQ9NYU1.
    BgeeiQ9NYU1.
    CleanExiHS_UGCGL2.
    GenevestigatoriQ9NYU1.

    Organism-specific databases

    HPAiHPA047955.

    Interactioni

    Subunit structurei

    Interacts with METTL23.1 Publication

    Protein-protein interaction databases

    BioGridi120875. 7 interactions.
    IntActiQ9NYU1. 2 interactions.
    STRINGi9606.ENSP00000365938.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9NYU1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1220 – 1516297GlucosyltransferaseAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1513 – 15164Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the glycosyltransferase 8 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG320899.
    HOGENOMiHOG000184622.
    HOVERGENiHBG079469.
    InParanoidiQ9NYU1.
    KOiK11718.
    OMAiVEYDAEI.
    OrthoDBiEOG75J0M7.
    PhylomeDBiQ9NYU1.
    TreeFamiTF300320.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR002495. Glyco_trans_8.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR009448. UDP-g_GGtrans.
    [Graphical view]
    PANTHERiPTHR11226. PTHR11226. 1 hit.
    PfamiPF01501. Glyco_transf_8. 1 hit.
    PF06427. UDP-g_GGTase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NYU1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAPAKATNVV RLLLGSTALW LSQLGSGTVA ASKSVTAHLA AKWPETPLLL     50
    EASEFMAEES NEKFWQFLET VQELAIYKQT ESDYSYYNLI LKKAGQFLDN 100
    LHINLLKFAF SIRAYSPAIQ MFQQIAADEP PPDGCNAFVV IHKKHTCKIN 150
    EIKKLLKKAA SRTRPYLFKG DHKFPTNKEN LPVVILYAEM GTRTFSAFHK 200
    VLSEKAQNEE ILYVLRHYIQ KPSSRKMYLS GYGVELAIKS TEYKALDDTQ 250
    VKTVTNTTVE DETETNEVQG FLFGKLKEIY SDLRDNLTAF QKYLIESNKQ 300
    MMPLKVWELQ DLSFQAASQI MSAPVYDSIK LMKDISQNFP IKARSLTRIA 350
    VNQHMREEIK ENQKDLQVRF KIQPGDARLF INGLRVDMDV YDAFSILDML 400
    KLEGKMMNGL RNLGINGEDM SKFLKLNSHI WEYTYVLDIR HSSIMWINDL 450
    ENDDLYITWP TSCQKLLKPV FPGSVPSIRR NFHNLVLFID PAQEYTLDFI 500
    KLADVFYSHE VPLRIGFVFI LNTDDEVDGA NDAGVALWRA FNYIAEEFDI 550
    SEAFISIVHM YQKVKKDQNI LTVDNVKSVL QNTFPHANIW DILGIHSKYD 600
    EERKAGASFY KMTGLGPLPQ ALYNGEPFKH EEMNIKELKM AVLQRMMDAS 650
    VYLQREVFLG TLNDRTNAID FLMDRNNVVP RINTLILRTN QQYLNLISTS 700
    VTADVEDFST FFFLDSQDKS AVIAKNMYYL TQDDESIISA VTLWIIADFD 750
    KPSGRKLLFN ALKHMKTSVH SRLGIIYNPT SKINEENTAI SRGILAAFLT 800
    QKNMFLRSFL GQLAKEEIAT AIYSGDKIKT FLIEGMDKNA FEKKYNTVGV 850
    NIFRTHQLFC QDVLKLRPGE MGIVSNGRFL GPLDEDFYAE DFYLLEKITF 900
    SNLGEKIKGI VENMGINANN MSDFIMKVDA LMSSVPKRAS RYDVTFLREN 950
    HSVIKTNPQE NDMFFNVIAI VDPLTREAQK MAQLLVVLGK IINMKIKLFM 1000
    NCRGRLSEAP LESFYRFVLE PELMSGANDV SSLGPVAKFL DIPESPLLIL 1050
    NMITPEGWLV ETVHSNCDLD NIHLKDTEKT VTAEYELEYL LLEGQCFDKV 1100
    TEQPPRGLQF TLGTKNKPAV VDTIVMAHHG YFQLKANPGA WILRLHQGKS 1150
    EDIYQIVGHE GTDSQADLED IIVVLNSFKS KILKVKVKKE TDKIKEDILT 1200
    DEDEKTKGLW DSIKSFTVSL HKENKKEKDV LNIFSVASGH LYERFLRIMM 1250
    LSVLRNTKTP VKFWLLKNYL SPTFKEVIPH MAKEYGFRYE LVQYRWPRWL 1300
    RQQTERQRII WGYKILFLDV LFPLAVDKII FVDADQIVRH DLKELRDFDL 1350
    DGAPYGYTPF CDSRREMDGY RFWKTGYWAS HLLRRKYHIS ALYVVDLKKF 1400
    RRIGAGDRLR SQYQALSQDP NSLSNLDQDL PNNMIYQVAI KSLPQDWLWC 1450
    ETWCDDESKQ RAKTIDLCNN PKTKESKLKA AARIVPEWVE YDAEIRQLLD 1500
    HLENKKQDTI LTHDEL 1516
    Length:1,516
    Mass (Da):174,735
    Last modified:November 2, 2010 - v4
    Checksum:iBD216896ECA54E4F
    GO
    Isoform 2 (identifier: Q9NYU1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         278-278: E → S
         279-1516: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:278
    Mass (Da):31,425
    Checksum:i8B2126D4F7309389
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti323 – 3231A → T.1 Publication
    Corresponds to variant rs12863903 [ dbSNP | Ensembl ].
    VAR_030006
    Natural varianti328 – 3281S → A.2 Publications
    Corresponds to variant rs816142 [ dbSNP | Ensembl ].
    VAR_030007
    Natural varianti821 – 8211A → T.1 Publication
    Corresponds to variant rs33949518 [ dbSNP | Ensembl ].
    VAR_055849
    Natural varianti865 – 8651K → R.
    Corresponds to variant rs35060832 [ dbSNP | Ensembl ].
    VAR_061196
    Natural varianti924 – 9241F → I.
    Corresponds to variant rs35780499 [ dbSNP | Ensembl ].
    VAR_055850
    Natural varianti994 – 9941M → L.2 Publications
    Corresponds to variant rs12876018 [ dbSNP | Ensembl ].
    VAR_030008
    Natural varianti1274 – 12741F → L.
    Corresponds to variant rs9525072 [ dbSNP | Ensembl ].
    VAR_055851
    Natural varianti1285 – 12851Y → F.
    Corresponds to variant rs35123499 [ dbSNP | Ensembl ].
    VAR_061197

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei278 – 2781E → S in isoform 2. 1 PublicationVSP_056319
    Alternative sequencei279 – 15161238Missing in isoform 2. 1 PublicationVSP_056320Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF227906 mRNA. Translation: AAF66233.2.
    AL136104
    , AL158192, AL162500, AL607038 Genomic DNA. Translation: CAH72447.1.
    AL158192
    , AL136104, AL162500, AL607038 Genomic DNA. Translation: CAI13708.1.
    AL607038
    , AL136104, AL158192, AL162500 Genomic DNA. Translation: CAI39962.1.
    AL162500
    , AL136104, AL158192, AL607038 Genomic DNA. Translation: CAI40146.1.
    BC032302 mRNA. Translation: AAH32302.1.
    BC125233 mRNA. Translation: AAI25234.1.
    AL133051 mRNA. Translation: CAB61378.1.
    CCDSiCCDS9480.1.
    PIRiT42654.
    RefSeqiNP_064506.3. NM_020121.3.
    UniGeneiHs.193226.
    Hs.656444.

    Genome annotation databases

    EnsembliENST00000376714; ENSP00000365904; ENSG00000102595.
    ENST00000376747; ENSP00000365938; ENSG00000102595.
    GeneIDi55757.
    KEGGihsa:55757.
    UCSCiuc001vmt.3. human.

    Polymorphism databases

    DMDMi311033544.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF227906 mRNA. Translation: AAF66233.2 .
    AL136104
    , AL158192 , AL162500 , AL607038 Genomic DNA. Translation: CAH72447.1 .
    AL158192
    , AL136104 , AL162500 , AL607038 Genomic DNA. Translation: CAI13708.1 .
    AL607038
    , AL136104 , AL158192 , AL162500 Genomic DNA. Translation: CAI39962.1 .
    AL162500
    , AL136104 , AL158192 , AL607038 Genomic DNA. Translation: CAI40146.1 .
    BC032302 mRNA. Translation: AAH32302.1 .
    BC125233 mRNA. Translation: AAI25234.1 .
    AL133051 mRNA. Translation: CAB61378.1 .
    CCDSi CCDS9480.1.
    PIRi T42654.
    RefSeqi NP_064506.3. NM_020121.3.
    UniGenei Hs.193226.
    Hs.656444.

    3D structure databases

    ProteinModelPortali Q9NYU1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120875. 7 interactions.
    IntActi Q9NYU1. 2 interactions.
    STRINGi 9606.ENSP00000365938.

    Protein family/group databases

    CAZyi GT24. Glycosyltransferase Family 24.

    PTM databases

    PhosphoSitei Q9NYU1.

    Polymorphism databases

    DMDMi 311033544.

    Proteomic databases

    MaxQBi Q9NYU1.
    PaxDbi Q9NYU1.
    PRIDEi Q9NYU1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000376714 ; ENSP00000365904 ; ENSG00000102595 .
    ENST00000376747 ; ENSP00000365938 ; ENSG00000102595 .
    GeneIDi 55757.
    KEGGi hsa:55757.
    UCSCi uc001vmt.3. human.

    Organism-specific databases

    CTDi 55757.
    GeneCardsi GC13M096453.
    H-InvDB HIX0011410.
    HGNCi HGNC:15664. UGGT2.
    HPAi HPA047955.
    MIMi 605898. gene.
    neXtProti NX_Q9NYU1.
    PharmGKBi PA38015.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG320899.
    HOGENOMi HOG000184622.
    HOVERGENi HBG079469.
    InParanoidi Q9NYU1.
    KOi K11718.
    OMAi VEYDAEI.
    OrthoDBi EOG75J0M7.
    PhylomeDBi Q9NYU1.
    TreeFami TF300320.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_25091. ER Quality Control Compartment (ERQC).

    Miscellaneous databases

    GenomeRNAii 55757.
    NextBioi 60767.
    PROi Q9NYU1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9NYU1.
    Bgeei Q9NYU1.
    CleanExi HS_UGCGL2.
    Genevestigatori Q9NYU1.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR002495. Glyco_trans_8.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR009448. UDP-g_GGtrans.
    [Graphical view ]
    PANTHERi PTHR11226. PTHR11226. 1 hit.
    Pfami PF01501. Glyco_transf_8. 1 hit.
    PF06427. UDP-g_GGTase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two homologues encoding human UDP-glucose:glycoprotein glucosyltransferase differ in mRNA expression and enzymatic activity."
      Arnold S.M., Fessler L.I., Fessler J.H., Kaufman R.J.
      Biochemistry 39:2149-2163(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANTS THR-323; ALA-328; THR-821 AND LEU-994.
      Tissue: Fetal liver.
    2. "The DNA sequence and analysis of human chromosome 13."
      Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
      Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS ALA-328 AND LEU-994.
      Tissue: Brain.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 151-1516 (ISOFORM 1).
      Tissue: Testis.
    5. "The noncatalytic portion of human UDP-glucose: glycoprotein glucosyltransferase I confers UDP-glucose binding and transferase function to the catalytic domain."
      Arnold S.M., Kaufman R.J.
      J. Biol. Chem. 278:43320-43328(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity."
      Cloutier P., Lavallee-Adam M., Faubert D., Blanchette M., Coulombe B.
      PLoS Genet. 9:E1003210-E1003210(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH METTL23.

    Entry informationi

    Entry nameiUGGG2_HUMAN
    AccessioniPrimary (citable) accession number: Q9NYU1
    Secondary accession number(s): A6NKL4
    , Q08AD0, Q5JQR8, Q8N5K0, Q9UFC4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 12, 2003
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 119 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 13
      Human chromosome 13: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3