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Protein

NF-kappa-B inhibitor-interacting Ras-like protein 1

Gene

NKIRAS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18GTPBy similarity8
Nucleotide bindingi61 – 65GTPBy similarity5
Nucleotide bindingi120 – 123GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • I-kappaB kinase/NF-kappaB signaling Source: UniProtKB

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1810476. RIP-mediated NFkB activation via ZBP1.
R-HSA-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-HSA-933542. TRAF6 mediated NF-kB activation.
SignaLinkiQ9NYS0.

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor-interacting Ras-like protein 1
Alternative name(s):
I-kappa-B-interacting Ras-like protein 1
Short name:
Kappa B-Ras protein 1
Short name:
KappaB-Ras1
Gene namesi
Name:NKIRAS1
Synonyms:KBRAS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17899. NKIRAS1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38T → A: Loss of function. 1 Publication1

Organism-specific databases

DisGeNETi28512.
OpenTargetsiENSG00000197885.
PharmGKBiPA134958823.

Polymorphism and mutation databases

BioMutaiNKIRAS1.
DMDMi74753075.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002256751 – 192NF-kappa-B inhibitor-interacting Ras-like protein 1Add BLAST192

Proteomic databases

EPDiQ9NYS0.
MaxQBiQ9NYS0.
PaxDbiQ9NYS0.
PeptideAtlasiQ9NYS0.
PRIDEiQ9NYS0.

PTM databases

iPTMnetiQ9NYS0.
PhosphoSitePlusiQ9NYS0.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000197885.
CleanExiHS_NKIRAS1.
ExpressionAtlasiQ9NYS0. baseline and differential.
GenevisibleiQ9NYS0. HS.

Organism-specific databases

HPAiHPA062521.

Interactioni

Subunit structurei

Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo. Forms a complex with NFKBIB and NF-kappa-B heterodimer (p50/NFKB1 and p65/RELA). Also interacts with c-Rel (REL).3 Publications

Protein-protein interaction databases

BioGridi118389. 7 interactors.
IntActiQ9NYS0. 1 interactor.
STRINGi9606.ENSP00000373411.

Structurei

3D structure databases

ProteinModelPortaliQ9NYS0.
SMRiQ9NYS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 93Interactions with NFKBIA and NFKBIBAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi35 – 43Effector region9

Domaini

In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Leu residues, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3883. Eukaryota.
ENOG4110VES. LUCA.
GeneTreeiENSGT00390000006495.
HOGENOMiHOG000230991.
HOVERGENiHBG105089.
InParanoidiQ9NYS0.
KOiK17197.
OMAiQWARGEK.
OrthoDBiEOG091G0IH8.
PhylomeDBiQ9NYS0.
TreeFamiTF314483.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51419. RAB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9NYS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKGCKVVVC GLLSVGKTAI LEQLLYGNHT IGMEDCETME DVYMASVETD
60 70 80 90 100
RGVKEQLHLY DTRGLQEGVE LPKHYFSFAD GFVLVYSVNN LESFQRVELL
110 120 130 140 150
KKEIDKFKDK KEVAIVVLGN KIDLSEQRQV DAEVAQQWAK SEKVRLWEVT
160 170 180 190
VTDRKTLIEP FTLLASKLSQ PQSKSSFPLP GRKNKGNSNS EN
Length:192
Mass (Da):21,643
Last modified:October 1, 2000 - v1
Checksum:i84948448BE0853B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95Q → R in BAB55341 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229839 mRNA. Translation: AAF34998.1.
AK027749 mRNA. Translation: BAB55341.1.
DQ314881 Genomic DNA. Translation: ABC40740.1.
BC066940 mRNA. Translation: AAH66940.1.
BC012145 mRNA. Translation: AAH12145.1.
CCDSiCCDS33717.1.
RefSeqiNP_065078.1. NM_020345.3.
XP_005265133.1. XM_005265076.3.
XP_005265134.1. XM_005265077.4.
XP_005265135.1. XM_005265078.3.
UniGeneiHs.173202.

Genome annotation databases

EnsembliENST00000388759; ENSP00000373411; ENSG00000197885.
ENST00000416026; ENSP00000394214; ENSG00000197885.
ENST00000421515; ENSP00000392307; ENSG00000197885.
ENST00000425478; ENSP00000400385; ENSG00000197885.
ENST00000443659; ENSP00000393785; ENSG00000197885.
ENST00000614374; ENSP00000483749; ENSG00000197885.
GeneIDi28512.
KEGGihsa:28512.
UCSCiuc003ccj.4. human.

Similar proteinsi

Entry informationi

Entry nameiKBRS1_HUMAN
AccessioniPrimary (citable) accession number: Q9NYS0
Secondary accession number(s): Q96K18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families