Q9NYR9 (KBRS2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NF-kappa-B inhibitor-interacting Ras-like protein 2

Alternative name(s):
I-kappa-B-interacting Ras-like protein 2
Short name=Kappa B-Ras protein 2
Short name=KappaB-Ras2

Gene names

Name:	NKIRAS2
Synonyms:	KBRAS2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB By similarity.
Subunit structure	Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo. Ref.1
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Widely expressed. Ref.1
Domain	In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
Sequence caution	The sequence BAB55006.1 differs from that shown. Reason: Absence of residues from position 133 within an exon that change the frame which is not the result of an alternative splicing.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	I-kappaB kinase/NF-kappaB cascade Non-traceable author statement Ref.1. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NYR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NYR9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     33-34: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9NYR9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     57-112: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q9NYR9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     57-97: VRFYDTRGLR...TDSRESFQRV → IAESLFSVWS...TSVTYRSSGV
     98-191: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	NF-kappa-B inhibitor-interacting Ras-like protein 2		PRO_0000225679
Regions
	Nucleotide binding	11 – 18	8	GTP By similarity
	Nucleotide binding	61 – 65	5	GTP By similarity
	Nucleotide binding	120 – 123	4	GTP By similarity
	Region	1 – 191	191	Small GTPase-like
	Motif	35 – 43	9	Effector region
Natural variations
	Alternative sequence	33 – 34	2	Missing in isoform 2.		VSP_017412
	Alternative sequence	57 – 112	56	Missing in isoform 3.		VSP_043151
	Alternative sequence	57 – 97	41	VRFYD…SFQRV → IAESLFSVWSCSRRRLTNPR TRRRSPSWSLATSVTYRSSG V in isoform 4.		VSP_044873
	Alternative sequence	98 – 191	94	Missing in isoform 4.		VSP_044874

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 27429230DA563342	FASTA	191	21,508
	10 20 30 40 50 60 MGKSCKVVVC GQASVGKTSI LEQLLYGNHV VGSEMIETQE DIYVGSIETD RGVREQVRFY 70 80 90 100 110 120 DTRGLRDGAE LPRHCFSCTD GYVLVYSTDS RESFQRVELL KKEIDKSKDK KEVTIVVLGN 130 140 150 160 170 180 KCDLQEQRRV DPDVAQHWAK SEKVKLWEVS VADRRSLLEP FVYLASKMTQ PQSKSAFPLS 190 RKNKGSGSLD G
	Isoform 2 [UniParc]. Checksum: 4857EEA02F788F38	FASTA	189	21,292
	10 20 30 40 50 60 MGKSCKVVVC GQASVGKTSI LEQLLYGNHV VGMIETQEDI YVGSIETDRG VREQVRFYDT 70 80 90 100 110 120 RGLRDGAELP RHCFSCTDGY VLVYSTDSRE SFQRVELLKK EIDKSKDKKE VTIVVLGNKC 130 140 150 160 170 180 DLQEQRRVDP DVAQHWAKSE KVKLWEVSVA DRRSLLEPFV YLASKMTQPQ SKSAFPLSRK NKGSGSLDG
	Isoform 3 [UniParc]. Checksum: 8B874EC8C7EF28E4	FASTA	135	14,910
	10 20 30 40 50 60 MGKSCKVVVC GQASVGKTSI LEQLLYGNHV VGSEMIETQE DIYVGSIETD RGVREQVTIV 70 80 90 100 110 120 VLGNKCDLQE QRRVDPDVAQ HWAKSEKVKL WEVSVADRRS LLEPFVYLAS KMTQPQSKSA 130 FPLSRKNKGS GSLDG
	Isoform 4 [UniParc]. Checksum: 4049375BE44AF8A5	FASTA	97	10,770
	10 20 30 40 50 60 MGKSCKVVVC GQASVGKTSI LEQLLYGNHV VGSEMIETQE DIYVGSIETD RGVREQIAES 70 80 90 LFSVWSCSRR RLTNPRTRRR SPSWSLATSV TYRSSGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A subclass of Ras proteins that regulate the degradation of IkappaB." Fenwick C., Na S.-Y., Voll R.E., Zhong H., Im S.-Y., Lee J.W., Ghosh S. Science 287:869-873(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH NFKBIA AND NFKBIB.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4). Tissue: Embryo and Thymus.
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[4]	NHLBI resequencing and genotyping service (RS&G) Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Muscle.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF229840 mRNA. Translation: AAF34999.1. AK027265 mRNA. Translation: BAB55006.1. Sequence problems. AK054571 mRNA. Translation: BAG51392.1. AK297975 mRNA. Translation: BAG60285.1. DB126967 mRNA. No translation available. AL137682 mRNA. Translation: CAB70873.2. DQ314882 Genomic DNA. Translation: ABC40741.1. AC105024 Genomic DNA. No translation available. CH471152 Genomic DNA. Translation: EAW60791.1. BC007450 mRNA. Translation: AAH07450.1. BC063498 mRNA. Translation: AAH63498.1.
IPI	IPI00021124. IPI00738016. IPI00789320. IPI00921434.
PIR	T46440.
RefSeq	NP_001001349.1. NM_001001349.2. NP_001138399.1. NM_001144927.1. NP_001138400.1. NM_001144928.1. NP_001138401.1. NM_001144929.1. NP_060065.2. NM_017595.5.
UniGene	Hs.632252.
3D structure databases
ProteinModelPortal	Q9NYR9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NYR9. 1 interaction.
STRING	9606.ENSP00000303580.
PTM databases
PhosphoSite	Q9NYR9.
Polymorphism databases
DMDM	74734716.
Proteomic databases
PaxDb	Q9NYR9.
PRIDE	Q9NYR9.
Protocols and materials databases
DNASU	28511.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307641; ENSP00000303580; ENSG00000168256. ENST00000393880; ENSP00000377458; ENSG00000168256. ENST00000393881; ENSP00000377459; ENSG00000168256. ENST00000393885; ENSP00000377463; ENSG00000168256. ENST00000449471; ENSP00000401976; ENSG00000168256. ENST00000479407; ENSP00000465633; ENSG00000168256. ENST00000561861; ENSP00000455221; ENSG00000260770. ENST00000562265; ENSP00000457014; ENSG00000260770. ENST00000563653; ENSP00000462262; ENSG00000260770. ENST00000568696; ENSP00000456949; ENSG00000260770. ENST00000569942; ENSP00000457286; ENSG00000260770. ENST00000570113; ENSP00000458054; ENSG00000260770.
GeneID	28511.
KEGG	hsa:28511.
UCSC	uc002hyq.3. human.
Organism-specific databases
CTD	28511.
GeneCards	GC17P040170.
HGNC	HGNC:17898. NKIRAS2.
MIM	604497. gene.
neXtProt	NX_Q9NYR9.
PharmGKB	PA134968907.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG302793.
HOGENOM	HOG000230991.
HOVERGEN	HBG105089.
OrthoDB	EOG4RV2SH.
PhylomeDB	Q9NYR9.
Gene expression databases
ArrayExpress	Q9NYR9.
Bgee	Q9NYR9.
CleanEx	HS_NKIRAS2.
Genevestigator	Q9NYR9.
GermOnline	ENSG00000168256. Homo sapiens.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NKIRAS2. human.
GenomeRNAi	28511.
NextBio	50970.
SOURCE	Search...

Entry information

Entry name

KBRS2_HUMAN

Accession

Primary (citable) accession number: Q9NYR9
Secondary accession number(s): A6NCZ5 , B3KNN0, B4DNM3, Q6PK52, Q96KC7, Q9NSX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.