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Protein

Protein Mis18-alpha

Gene

MIS18A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851ZincPROSITE-ProRule annotation
Metal bindingi88 – 881ZincPROSITE-ProRule annotation
Metal bindingi141 – 1411ZincPROSITE-ProRule annotation
Metal bindingi144 – 1441ZincPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Mis18-alpha
Alternative name(s):
FAPP1-associated protein 1
Gene namesi
Name:MIS18A
Synonyms:C21orf45, C21orf46, FASP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

HGNCiHGNC:1286. MIS18A.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome 1 Publication
  • Chromosomecentromere 1 Publication

  • Note: Associated with centromeres in interphase cells, from late anaphase to the G1 phase. Not detected on centromeres during earlier phases of mitosis. Associated with chromatin.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi82 – 821V → E: Abolishes interaction with OIP5; when associated with D-176. 1 Publication
Mutagenesisi85 – 851C → A: Abolishes location at the centromere. 1 Publication
Mutagenesisi88 – 881C → A: Abolishes location at the centromere. 1 Publication
Mutagenesisi134 – 1341C → A: No effect. 1 Publication
Mutagenesisi141 – 1411C → A: Abolishes location at the centromere. 1 Publication
Mutagenesisi144 – 1441C → A: Abolishes location at the centromere. 1 Publication
Mutagenesisi176 – 1761Y → D: Abolishes interaction with OIP5; when associated with E-82. 1 Publication

Organism-specific databases

PharmGKBiPA25840.

Polymorphism and mutation databases

BioMutaiMIS18A.
DMDMi12229716.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Protein Mis18-alphaPRO_0000079515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361PhosphoserineCombined sources
Modified residuei39 – 391PhosphoserineCombined sources
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei233 – 2331PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NYP9.
MaxQBiQ9NYP9.
PaxDbiQ9NYP9.
PeptideAtlasiQ9NYP9.
PRIDEiQ9NYP9.

PTM databases

iPTMnetiQ9NYP9.
PhosphoSiteiQ9NYP9.

Expressioni

Tissue specificityi

Detected in testis.1 Publication

Gene expression databases

BgeeiQ9NYP9.
CleanExiHS_C21orf45.
GenevisibleiQ9NYP9. HS.

Organism-specific databases

HPAiHPA018286.
HPA020585.

Interactioni

Subunit structurei

Homodimer, and heterodimer with OIP5/MIS18B (PubMed:17199038, PubMed:26921242). Identified in a complex containing MIS18A, OIP5/MIS18B, MIS18BP1, RBBP7 and RBBP4 (PubMed:17199038).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AIMP2Q131553EBI-1104552,EBI-745226
BRD8Q9H0E9-23EBI-1104552,EBI-10304361
MBIPQ9NS73-53EBI-1104552,EBI-10182361
OIP5O4348211EBI-1104552,EBI-536879

Protein-protein interaction databases

BioGridi119875. 47 interactions.
IntActiQ9NYP9. 17 interactions.
STRINGi9606.ENSP00000290130.

Structurei

3D structure databases

ProteinModelPortaliQ9NYP9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 17899Mis18PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the mis18 family.PROSITE-ProRule annotation
Contains 1 Mis18 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IKYV. Eukaryota.
ENOG41125JV. LUCA.
GeneTreeiENSGT00390000009777.
HOGENOMiHOG000059535.
HOVERGENiHBG108147.
InParanoidiQ9NYP9.
KOiK11564.
OMAiEDSSRHQ.
OrthoDBiEOG70KGSQ.
PhylomeDBiQ9NYP9.
TreeFamiTF333200.

Family and domain databases

InterProiIPR004910. Yippee/Mis18/Cereblon.
[Graphical view]
PfamiPF03226. Yippee-Mis18. 1 hit.
[Graphical view]
PROSITEiPS51793. MIS18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9NYP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVRSLRCS RGCAGGCECG DKGKCSDSSL LGKRLSEDSS RHQLLQKWAS
60 70 80 90 100
MWSSMSEDAS VADMERAQLE EEAAAAEERP LVFLCSGCRR PLGDSLSWVA
110 120 130 140 150
SQEDTNCILL RCVSCNVSVD KEQKLSKREK ENGCVLETLC CAGCSLNLGY
160 170 180 190 200
VYRCTPKNLD YKRDLFCLSV EAIESYVLGS SEKQIVSEDK ELFNLESRVE
210 220 230
IEKSLTQMED VLKALQMKLW EAESKLSFAT CKS
Length:233
Mass (Da):25,863
Last modified:October 1, 2000 - v1
Checksum:iA07522806C4B6221
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF387845 mRNA. Translation: AAK70498.1.
AF231921 mRNA. Translation: AAF72945.1.
AK075281 mRNA. Translation: BAC11517.1.
AK312073 mRNA. Translation: BAG35009.1.
CH471079 Genomic DNA. Translation: EAX09881.1.
BC042917 mRNA. Translation: AAH42917.1.
CCDSiCCDS13611.1.
RefSeqiNP_061817.1. NM_018944.2.
UniGeneiHs.190518.

Genome annotation databases

EnsembliENST00000290130; ENSP00000290130; ENSG00000159055.
GeneIDi54069.
KEGGihsa:54069.
UCSCiuc002ypi.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF387845 mRNA. Translation: AAK70498.1.
AF231921 mRNA. Translation: AAF72945.1.
AK075281 mRNA. Translation: BAC11517.1.
AK312073 mRNA. Translation: BAG35009.1.
CH471079 Genomic DNA. Translation: EAX09881.1.
BC042917 mRNA. Translation: AAH42917.1.
CCDSiCCDS13611.1.
RefSeqiNP_061817.1. NM_018944.2.
UniGeneiHs.190518.

3D structure databases

ProteinModelPortaliQ9NYP9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119875. 47 interactions.
IntActiQ9NYP9. 17 interactions.
STRINGi9606.ENSP00000290130.

PTM databases

iPTMnetiQ9NYP9.
PhosphoSiteiQ9NYP9.

Polymorphism and mutation databases

BioMutaiMIS18A.
DMDMi12229716.

Proteomic databases

EPDiQ9NYP9.
MaxQBiQ9NYP9.
PaxDbiQ9NYP9.
PeptideAtlasiQ9NYP9.
PRIDEiQ9NYP9.

Protocols and materials databases

DNASUi54069.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000290130; ENSP00000290130; ENSG00000159055.
GeneIDi54069.
KEGGihsa:54069.
UCSCiuc002ypi.4. human.

Organism-specific databases

CTDi54069.
GeneCardsiMIS18A.
HGNCiHGNC:1286. MIS18A.
HPAiHPA018286.
HPA020585.
neXtProtiNX_Q9NYP9.
PharmGKBiPA25840.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKYV. Eukaryota.
ENOG41125JV. LUCA.
GeneTreeiENSGT00390000009777.
HOGENOMiHOG000059535.
HOVERGENiHBG108147.
InParanoidiQ9NYP9.
KOiK11564.
OMAiEDSSRHQ.
OrthoDBiEOG70KGSQ.
PhylomeDBiQ9NYP9.
TreeFamiTF333200.

Enzyme and pathway databases

ReactomeiR-HSA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.

Miscellaneous databases

ChiTaRSiMIS18A. human.
GeneWikiiC21orf45.
GenomeRNAii54069.
PROiQ9NYP9.

Gene expression databases

BgeeiQ9NYP9.
CleanExiHS_C21orf45.
GenevisibleiQ9NYP9. HS.

Family and domain databases

InterProiIPR004910. Yippee/Mis18/Cereblon.
[Graphical view]
PfamiPF03226. Yippee-Mis18. 1 hit.
[Graphical view]
PROSITEiPS51793. MIS18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of FAPP1 interacting proteins."
    Dowler S.J.
    Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Criteria for gene identification and features of genome organization: analysis of 6.5 Mb of DNA sequence from human chromosome 21."
    Slavov D., Hattori M., Sakaki Y., Rosenthal A., Shimizu N., Minoshima S., Kudoh J., Yaspo M.-L., Ramser J., Reinhardt R., Reimer C., Clancy K., Rynditch A., Gardiner K.
    Gene 247:215-232(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Blood.
  7. "Priming of centromere for CENP-A recruitment by human hMis18alpha, hMis18beta, and M18BP1."
    Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M.
    Dev. Cell 12:17-30(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH OIP5 AND MIS18BP1, IDENTIFICATION IN A COMPLEX CONTAINING MIS18A; OIP5; MIS18BP1; RBBP7 AND RBBP4, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF CYS-85; CYS-88; CYS-134; CYS-141 AND CYS-144, TISSUE SPECIFICITY.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-233, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36; SER-39 AND SER-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  11. "Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization."
    Subramanian L., Medina-Pritchard B., Barton R., Spiller F., Kulasegaran-Shylini R., Radaviciute G., Allshire R.C., Arockia Jeyaprakash A.
    EMBO Rep. 17:496-507(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, MUTAGENESIS OF VAL-82 AND TYR-176.

Entry informationi

Entry nameiMS18A_HUMAN
AccessioniPrimary (citable) accession number: Q9NYP9
Secondary accession number(s): B2R562, Q542Z0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.