Q9NYK1 (TLR7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toll-like receptor 7

Gene names

Name:	TLR7
ORF Names:	UNQ248/PRO285

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1049 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response By similarity. Ref.6
Subunit structure	Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1 By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Endosome By similarity. Lysosome By similarity. Cytoplasmic vesicle › phagosome. Note: Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist By similarity.
Tissue specificity	Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.
Sequence similarities	Belongs to the Toll-like receptor family. Contains 27 LRR (leucine-rich) repeats. Contains 1 TIR domain.

Ontologies

Keywords
Biological process	Immunity Inflammatory response Innate immunity
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Endosome Lysosome Membrane
Coding sequence diversity	Polymorphism
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	I-kappaB phosphorylation Inferred from direct assay PubMed 19740627. Source: BHF-UCL MyD88-dependent toll-like receptor signaling pathway Inferred from electronic annotation. Source: InterPro cellular response to mechanical stimulus Inferred from expression pattern PubMed 19593445. Source: UniProtKB defense response to virus Inferred from mutant phenotype PubMed 16188996. Source: UniProtKB inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Traceable author statement. Source: Reactome microglial cell activation involved in immune response Inferred from electronic annotation. Source: Compara pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response Inferred from electronic annotation. Source: Compara positive regulation of NF-kappaB import into nucleus Inferred from direct assay PubMed 19740627. Source: BHF-UCL positive regulation of chemokine production Inferred from direct assay PubMed 19740627. Source: BHF-UCL positive regulation of inflammatory response Inferred by curator PubMed 19740627. Source: BHF-UCL positive regulation of interferon-alpha biosynthetic process Inferred from direct assay PubMed 16286015. Source: UniProtKB positive regulation of interferon-beta biosynthetic process Inferred from direct assay PubMed 16286015. Source: UniProtKB positive regulation of interferon-gamma biosynthetic process Inferred from direct assay PubMed 16286015. Source: UniProtKB positive regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-8 biosynthetic process Inferred from mutant phenotype PubMed 16188996. Source: UniProtKB positive regulation of interleukin-8 production Inferred from direct assay PubMed 19740627. Source: BHF-UCL response to chemical stimulus Inferred from electronic annotation. Source: Compara toll-like receptor 7 signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome early phagosome Inferred from sequence or structural similarity. Source: UniProtKB endolysosome membrane Traceable author statement. Source: Reactome endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 19740627. Source: BHF-UCL
Molecular_function	double-stranded RNA binding Inferred from direct assay PubMed 16111635. Source: UniProtKB drug binding Inferred from electronic annotation. Source: Compara siRNA binding Inferred from sequence or structural similarity. Source: UniProtKB single-stranded RNA binding Inferred from sequence or structural similarity. Source: UniProtKB transmembrane signaling receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 1049	1023	Toll-like receptor 7		PRO_0000034733
Regions
	Topological domain	27 – 839	813	Extracellular Potential
	Transmembrane	840 – 860	21	Helical; Potential
	Topological domain	861 – 1049	189	Cytoplasmic Potential
	Repeat	43 – 64	22	LRR 1
	Repeat	65 – 87	23	LRR 2
	Repeat	110 – 126	17	LRR 3
	Repeat	127 – 149	23	LRR 4
	Repeat	151 – 170	20	LRR 5
	Repeat	171 – 195	25	LRR 6
	Repeat	203 – 226	24	LRR 7
	Repeat	228 – 247	20	LRR 8
	Repeat	248 – 275	28	LRR 9
	Repeat	289 – 312	24	LRR 10
	Repeat	314 – 337	24	LRR 11
	Repeat	339 – 368	30	LRR 12
	Repeat	369 – 392	24	LRR 13
	Repeat	396 – 419	24	LRR 14
	Repeat	421 – 443	23	LRR 15
	Repeat	492 – 515	24	LRR 16
	Repeat	516 – 540	25	LRR 17
	Repeat	541 – 564	24	LRR 18
	Repeat	566 – 588	23	LRR 19
	Repeat	595 – 618	24	LRR 20
	Repeat	619 – 644	26	LRR 21
	Repeat	649 – 672	24	LRR 22
	Repeat	674 – 697	24	LRR 23
	Repeat	698 – 721	24	LRR 24
	Repeat	723 – 745	23	LRR 25
	Repeat	746 – 769	24	LRR 26
	Repeat	772 – 795	24	LRR 27
	Domain	889 – 1036	148	TIR
Amino acid modifications
	Modified residue	1010	1	Phosphoserine By similarity
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	167	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Glycosylation	361	1	N-linked (GlcNAc...) Potential
	Glycosylation	413	1	N-linked (GlcNAc...) Potential
	Glycosylation	488	1	N-linked (GlcNAc...) Potential
	Glycosylation	523	1	N-linked (GlcNAc...) Potential
	Glycosylation	534	1	N-linked (GlcNAc...) Potential
	Glycosylation	590	1	N-linked (GlcNAc...) Potential
	Glycosylation	679	1	N-linked (GlcNAc...) Potential
	Glycosylation	720	1	N-linked (GlcNAc...) Potential
	Glycosylation	799	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	11	1	Q → L. Ref.3 Corresponds to variant rs179008 [ dbSNP | Ensembl ].		VAR_034554
	Natural variant	448	1	A → V. Corresponds to variant rs5743781 [ dbSNP | Ensembl ].		VAR_024665
Experimental info
	Sequence conflict	725	1	L → H in AAF78035. Ref.2
	Sequence conflict	738	1	L → P in AAF78035. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9NYK1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8C701E9E437F2721	FASTA	1,049	120,922
	10 20 30 40 50 60 MVFPMWTLKR QILILFNIIL ISKLLGARWF PKTLPCDVTL DVPKNHVIVD CTDKHLTEIP 70 80 90 100 110 120 GGIPTNTTNL TLTINHIPDI SPASFHRLDH LVEIDFRCNC VPIPLGSKNN MCIKRLQIKP 130 140 150 160 170 180 RSFSGLTYLK SLYLDGNQLL EIPQGLPPSL QLLSLEANNI FSIRKENLTE LANIEILYLG 190 200 210 220 230 240 QNCYYRNPCY VSYSIEKDAF LNLTKLKVLS LKDNNVTAVP TVLPSTLTEL YLYNNMIAKI 250 260 270 280 290 300 QEDDFNNLNQ LQILDLSGNC PRCYNAPFPC APCKNNSPLQ IPVNAFDALT ELKVLRLHSN 310 320 330 340 350 360 SLQHVPPRWF KNINKLQELD LSQNFLAKEI GDAKFLHFLP SLIQLDLSFN FELQVYRASM 370 380 390 400 410 420 NLSQAFSSLK SLKILRIRGY VFKELKSFNL SPLHNLQNLE VLDLGTNFIK IANLSMFKQF 430 440 450 460 470 480 KRLKVIDLSV NKISPSGDSS EVGFCSNART SVESYEPQVL EQLHYFRYDK YARSCRFKNK 490 500 510 520 530 540 EASFMSVNES CYKYGQTLDL SKNSIFFVKS SDFQHLSFLK CLNLSGNLIS QTLNGSEFQP 550 560 570 580 590 600 LAELRYLDFS NNRLDLLHST AFEELHKLEV LDISSNSHYF QSEGITHMLN FTKNLKVLQK 610 620 630 640 650 660 LMMNDNDISS STSRTMESES LRTLEFRGNH LDVLWREGDN RYLQLFKNLL KLEELDISKN 670 680 690 700 710 720 SLSFLPSGVF DGMPPNLKNL SLAKNGLKSF SWKKLQCLKN LETLDLSHNQ LTTVPERLSN 730 740 750 760 770 780 CSRSLKNLIL KNNQIRSLTK YFLQDAFQLR YLDLSSNKIQ MIQKTSFPEN VLNNLKMLLL 790 800 810 820 830 840 HHNRFLCTCD AVWFVWWVNH TEVTIPYLAT DVTCVGPGAH KGQSVISLDL YTCELDLTNL 850 860 870 880 890 900 ILFSLSISVS LFLMVMMTAS HLYFWDVWYI YHFCKAKIKG YQRLISPDCC YDAFIVYDTK 910 920 930 940 950 960 DPAVTEWVLA ELVAKLEDPR EKHFNLCLEE RDWLPGQPVL ENLSQSIQLS KKTVFVMTDK 970 980 990 1000 1010 1020 YAKTENFKIA FYLSHQRLMD EKVDVIILIF LEKPFQKSKF LQLRKRLCGS SVLEWPTNPQ 1030 1040 AHPYFWQCLK NALATDNHVA YSQVFKETV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three novel mammalian Toll-like receptors: gene structure, expression, and evolution." Du X., Poltorak A., Wei Y., Beutler B. Eur. Cytokine Netw. 11:362-371(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Cloning and characterization of a sub-family of human Toll-like receptors: hTLR7, hTLR8 and hTLR9." Chuang T.-H., Ulevitch R.J. Eur. Cytokine Netw. 11:372-378(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	"The heterogeneous allelic repertoire of human Toll-Like receptor (TLR) genes." Georgel P., Macquin C., Bahram S. PLoS ONE 4:E7803-E7803(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-11.
[4]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[6]	"TLR7 is involved in sequence-specific sensing of single-stranded RNAs in human macrophages." Gantier M.P., Tong S., Behlke M.A., Xu D., Phipps S., Foster P.S., Williams B.R. J. Immunol. 180:2117-2124(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF240467 mRNA. Translation: AAF60188.1. AF245702 mRNA. Translation: AAF78035.1. DQ022185 Genomic DNA. Translation: AAZ99026.1. AY358292 mRNA. Translation: AAQ88659.1. BC033651 mRNA. Translation: AAH33651.1.
IPI	IPI00009812.
RefSeq	NP_057646.1. NM_016562.3.
UniGene	Hs.659215.
3D structure databases
ProteinModelPortal	Q9NYK1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NYK1. 3 interactions.
STRING	9606.ENSP00000370034.
PTM databases
PhosphoSite	Q9NYK1.
Polymorphism databases
DMDM	20140876.
Proteomic databases
PaxDb	Q9NYK1.
PeptideAtlas	Q9NYK1.
PRIDE	Q9NYK1.
Protocols and materials databases
DNASU	51284.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000380659; ENSP00000370034; ENSG00000196664.
GeneID	51284.
KEGG	hsa:51284.
UCSC	uc004cvc.3. human.
Organism-specific databases
CTD	51284.
GeneCards	GC0XP012885.
HGNC	HGNC:15631. TLR7.
MIM	300365. gene.
neXtProt	NX_Q9NYK1.
PharmGKB	PA38008.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG286662.
HOGENOM	HOG000230468.
HOVERGEN	HBG018601.
InParanoid	Q9NYK1.
KO	K05404.
OMA	AFYLSHQ.
OrthoDB	EOG4BCDM5.
PhylomeDB	Q9NYK1.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q9NYK1.
Bgee	Q9NYK1.
CleanEx	HS_TLR7.
Genevestigator	Q9NYK1.
GermOnline	ENSG00000196664. Homo sapiens.
Family and domain databases
InterPro	IPR000483. Cys-rich_flank_reg_C. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000372. LRR-contain_N. IPR026906. LRR_5. IPR000157. TIR_dom. IPR026880. TLR7. [Graphical view]
PANTHER	PTHR24365:SF1. PTHR24365:SF1. 1 hit.
Pfam	PF00560. LRR_1. 3 hits. PF13306. LRR_5. 1 hit. PF01582. TIR. 1 hit. [Graphical view]
SMART	SM00369. LRR_TYP. 3 hits. SM00082. LRRCT. 1 hit. SM00013. LRRNT. 1 hit. [Graphical view]
SUPFAM	SSF52200. TIR. 1 hit.
PROSITE	PS51450. LRR. 18 hits. PS50104. TIR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9NYK1.
ChEMBL	CHEMBL5936.
DrugBank	DB00724. Imiquimod.
GenomeRNAi	51284.
NextBio	35489043.
SOURCE	Search...

Entry information

Entry name

TLR7_HUMAN

Accession

Primary (citable) accession number: Q9NYK1
Secondary accession number(s): D1CS69, Q9NR98

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.