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Q9NYK1 (TLR7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toll-like receptor 7
Gene names
Name:TLR7
ORF Names:UNQ248/PRO285
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1049 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response By similarity. Ref.6

Subunit structure

Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1 By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity. Endosome By similarity. Lysosome By similarity. Cytoplasmic vesiclephagosome. Note: Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist By similarity.

Tissue specificity

Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

Sequence similarities

Belongs to the Toll-like receptor family.

Contains 27 LRR (leucine-rich) repeats.

Contains 1 TIR domain.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentCytoplasmic vesicle
Endoplasmic reticulum
Endosome
Lysosome
Membrane
   Coding sequence diversityPolymorphism
   DomainLeucine-rich repeat
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processI-kappaB phosphorylation

Inferred from direct assay PubMed 19740627. Source: BHF-UCL

MyD88-dependent toll-like receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

cellular response to mechanical stimulus

Inferred from expression pattern PubMed 19593445. Source: UniProtKB

defense response to virus

Inferred from mutant phenotype PubMed 16188996. Source: UniProtKB

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Traceable author statement. Source: Reactome

microglial cell activation involved in immune response

Inferred from electronic annotation. Source: Ensembl

pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response

Inferred from electronic annotation. Source: Ensembl

positive regulation of NF-kappaB import into nucleus

Inferred from direct assay PubMed 19740627. Source: BHF-UCL

positive regulation of chemokine production

Inferred from direct assay PubMed 19740627. Source: BHF-UCL

positive regulation of inflammatory response

Inferred by curator PubMed 19740627. Source: BHF-UCL

positive regulation of interferon-alpha biosynthetic process

Inferred from direct assay PubMed 16286015. Source: UniProtKB

positive regulation of interferon-beta biosynthetic process

Inferred from direct assay PubMed 16286015. Source: UniProtKB

positive regulation of interferon-gamma biosynthetic process

Inferred from direct assay PubMed 16286015. Source: UniProtKB

positive regulation of interleukin-6 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of interleukin-8 biosynthetic process

Inferred from mutant phenotype PubMed 16188996. Source: UniProtKB

positive regulation of interleukin-8 production

Inferred from direct assay PubMed 19740627. Source: BHF-UCL

toll-like receptor 7 signaling pathway

Inferred from electronic annotation. Source: Ensembl

toll-like receptor 9 signaling pathway

Traceable author statement. Source: Reactome

toll-like receptor signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentGolgi membrane

Traceable author statement. Source: Reactome

cytoplasm

Inferred from direct assay PubMed 19740627. Source: BHF-UCL

early phagosome

Inferred from sequence or structural similarity. Source: UniProtKB

endolysosome membrane

Traceable author statement. Source: Reactome

endoplasmic reticulum

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

endosome

Inferred from sequence or structural similarity. Source: UniProtKB

endosome membrane

Traceable author statement. Source: Reactome

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lysosome

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from direct assay PubMed 19740627. Source: BHF-UCL

receptor complex

Inferred from direct assay PubMed 23382219. Source: MGI

   Molecular_functiondouble-stranded RNA binding

Inferred from direct assay PubMed 16111635. Source: UniProtKB

drug binding

Inferred from electronic annotation. Source: Ensembl

siRNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

single-stranded RNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

transmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 10491023Toll-like receptor 7
PRO_0000034733

Regions

Topological domain27 – 839813Extracellular Potential
Transmembrane840 – 86021Helical; Potential
Topological domain861 – 1049189Cytoplasmic Potential
Repeat43 – 6422LRR 1
Repeat65 – 8723LRR 2
Repeat110 – 12617LRR 3
Repeat127 – 14923LRR 4
Repeat151 – 17020LRR 5
Repeat171 – 19525LRR 6
Repeat203 – 22624LRR 7
Repeat228 – 24720LRR 8
Repeat248 – 27528LRR 9
Repeat289 – 31224LRR 10
Repeat314 – 33724LRR 11
Repeat339 – 36830LRR 12
Repeat369 – 39224LRR 13
Repeat396 – 41924LRR 14
Repeat421 – 44323LRR 15
Repeat492 – 51524LRR 16
Repeat516 – 54025LRR 17
Repeat541 – 56424LRR 18
Repeat566 – 58823LRR 19
Repeat595 – 61824LRR 20
Repeat619 – 64426LRR 21
Repeat649 – 67224LRR 22
Repeat674 – 69724LRR 23
Repeat698 – 72124LRR 24
Repeat723 – 74523LRR 25
Repeat746 – 76924LRR 26
Repeat772 – 79524LRR 27
Domain889 – 1036148TIR

Amino acid modifications

Glycosylation661N-linked (GlcNAc...) Potential
Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation1671N-linked (GlcNAc...) Potential
Glycosylation2021N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Glycosylation4131N-linked (GlcNAc...) Potential
Glycosylation4881N-linked (GlcNAc...) Potential
Glycosylation5231N-linked (GlcNAc...) Potential
Glycosylation5341N-linked (GlcNAc...) Potential
Glycosylation5901N-linked (GlcNAc...) Potential
Glycosylation6791N-linked (GlcNAc...) Potential
Glycosylation7201N-linked (GlcNAc...) Potential
Glycosylation7991N-linked (GlcNAc...) Potential

Natural variations

Natural variant111Q → L. Ref.3
Corresponds to variant rs179008 [ dbSNP | Ensembl ].
VAR_034554
Natural variant4481A → V.
Corresponds to variant rs5743781 [ dbSNP | Ensembl ].
VAR_024665

Experimental info

Sequence conflict7251L → H in AAF78035. Ref.2
Sequence conflict7381L → P in AAF78035. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9NYK1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 8C701E9E437F2721

FASTA1,049120,922
        10         20         30         40         50         60 
MVFPMWTLKR QILILFNIIL ISKLLGARWF PKTLPCDVTL DVPKNHVIVD CTDKHLTEIP 

        70         80         90        100        110        120 
GGIPTNTTNL TLTINHIPDI SPASFHRLDH LVEIDFRCNC VPIPLGSKNN MCIKRLQIKP 

       130        140        150        160        170        180 
RSFSGLTYLK SLYLDGNQLL EIPQGLPPSL QLLSLEANNI FSIRKENLTE LANIEILYLG 

       190        200        210        220        230        240 
QNCYYRNPCY VSYSIEKDAF LNLTKLKVLS LKDNNVTAVP TVLPSTLTEL YLYNNMIAKI 

       250        260        270        280        290        300 
QEDDFNNLNQ LQILDLSGNC PRCYNAPFPC APCKNNSPLQ IPVNAFDALT ELKVLRLHSN 

       310        320        330        340        350        360 
SLQHVPPRWF KNINKLQELD LSQNFLAKEI GDAKFLHFLP SLIQLDLSFN FELQVYRASM 

       370        380        390        400        410        420 
NLSQAFSSLK SLKILRIRGY VFKELKSFNL SPLHNLQNLE VLDLGTNFIK IANLSMFKQF 

       430        440        450        460        470        480 
KRLKVIDLSV NKISPSGDSS EVGFCSNART SVESYEPQVL EQLHYFRYDK YARSCRFKNK 

       490        500        510        520        530        540 
EASFMSVNES CYKYGQTLDL SKNSIFFVKS SDFQHLSFLK CLNLSGNLIS QTLNGSEFQP 

       550        560        570        580        590        600 
LAELRYLDFS NNRLDLLHST AFEELHKLEV LDISSNSHYF QSEGITHMLN FTKNLKVLQK 

       610        620        630        640        650        660 
LMMNDNDISS STSRTMESES LRTLEFRGNH LDVLWREGDN RYLQLFKNLL KLEELDISKN 

       670        680        690        700        710        720 
SLSFLPSGVF DGMPPNLKNL SLAKNGLKSF SWKKLQCLKN LETLDLSHNQ LTTVPERLSN 

       730        740        750        760        770        780 
CSRSLKNLIL KNNQIRSLTK YFLQDAFQLR YLDLSSNKIQ MIQKTSFPEN VLNNLKMLLL 

       790        800        810        820        830        840 
HHNRFLCTCD AVWFVWWVNH TEVTIPYLAT DVTCVGPGAH KGQSVISLDL YTCELDLTNL 

       850        860        870        880        890        900 
ILFSLSISVS LFLMVMMTAS HLYFWDVWYI YHFCKAKIKG YQRLISPDCC YDAFIVYDTK 

       910        920        930        940        950        960 
DPAVTEWVLA ELVAKLEDPR EKHFNLCLEE RDWLPGQPVL ENLSQSIQLS KKTVFVMTDK 

       970        980        990       1000       1010       1020 
YAKTENFKIA FYLSHQRLMD EKVDVIILIF LEKPFQKSKF LQLRKRLCGS SVLEWPTNPQ 

      1030       1040 
AHPYFWQCLK NALATDNHVA YSQVFKETV 

« Hide

References

« Hide 'large scale' references
[1]"Three novel mammalian Toll-like receptors: gene structure, expression, and evolution."
Du X., Poltorak A., Wei Y., Beutler B.
Eur. Cytokine Netw. 11:362-371(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Cloning and characterization of a sub-family of human Toll-like receptors: hTLR7, hTLR8 and hTLR9."
Chuang T.-H., Ulevitch R.J.
Eur. Cytokine Netw. 11:372-378(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[3]"The heterogeneous allelic repertoire of human Toll-Like receptor (TLR) genes."
Georgel P., Macquin C., Bahram S.
PLoS ONE 4:E7803-E7803(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-11.
[4]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[6]"TLR7 is involved in sequence-specific sensing of single-stranded RNAs in human macrophages."
Gantier M.P., Tong S., Behlke M.A., Xu D., Phipps S., Foster P.S., Williams B.R.
J. Immunol. 180:2117-2124(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF240467 mRNA. Translation: AAF60188.1.
AF245702 mRNA. Translation: AAF78035.1.
DQ022185 Genomic DNA. Translation: AAZ99026.1.
AY358292 mRNA. Translation: AAQ88659.1.
BC033651 mRNA. Translation: AAH33651.1.
RefSeqNP_057646.1. NM_016562.3.
UniGeneHs.659215.

3D structure databases

ProteinModelPortalQ9NYK1.
SMRQ9NYK1. Positions 32-847, 891-1035.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119436. 2 interactions.
IntActQ9NYK1. 3 interactions.
STRING9606.ENSP00000370034.

Chemistry

BindingDBQ9NYK1.
ChEMBLCHEMBL2111471.
DrugBankDB00724. Imiquimod.
GuidetoPHARMACOLOGY1757.

PTM databases

PhosphoSiteQ9NYK1.

Polymorphism databases

DMDM20140876.

Proteomic databases

PaxDbQ9NYK1.
PeptideAtlasQ9NYK1.
PRIDEQ9NYK1.

Protocols and materials databases

DNASU51284.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000380659; ENSP00000370034; ENSG00000196664.
GeneID51284.
KEGGhsa:51284.
UCSCuc004cvc.3. human.

Organism-specific databases

CTD51284.
GeneCardsGC0XP012885.
HGNCHGNC:15631. TLR7.
HPAHPA059613.
MIM300365. gene.
neXtProtNX_Q9NYK1.
PharmGKBPA38008.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG286662.
HOGENOMHOG000230468.
HOVERGENHBG018601.
InParanoidQ9NYK1.
KOK05404.
OMAAFYLSHQ.
OrthoDBEOG7VB2DM.
PhylomeDBQ9NYK1.
TreeFamTF325595.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressQ9NYK1.
BgeeQ9NYK1.
CleanExHS_TLR7.
GenevestigatorQ9NYK1.

Family and domain databases

Gene3D3.40.50.10140. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR026880. TLR7.
[Graphical view]
PANTHERPTHR24365:SF176. PTHR24365:SF176. 1 hit.
PfamPF00560. LRR_1. 3 hits.
PF13306. LRR_5. 1 hit.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMSSF52200. SSF52200. 1 hit.
PROSITEPS51450. LRR. 18 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTLR_7.
GenomeRNAi51284.
NextBio35489043.
PROQ9NYK1.
SOURCESearch...

Entry information

Entry nameTLR7_HUMAN
AccessionPrimary (citable) accession number: Q9NYK1
Secondary accession number(s): D1CS69, Q9NR98
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM