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Protein

Toll-like receptor 7

Gene

TLR7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).By similarity

GO - Molecular functioni

  1. double-stranded RNA binding Source: UniProtKB
  2. drug binding Source: Ensembl
  3. single-stranded RNA binding Source: UniProtKB
  4. siRNA binding Source: UniProtKB
  5. transmembrane signaling receptor activity Source: InterPro

GO - Biological processi

  1. cellular response to mechanical stimulus Source: UniProtKB
  2. defense response to virus Source: UniProtKB
  3. I-kappaB phosphorylation Source: BHF-UCL
  4. inflammatory response Source: UniProtKB-KW
  5. innate immune response Source: Reactome
  6. microglial cell activation Source: Ensembl
  7. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
  8. positive regulation of chemokine production Source: BHF-UCL
  9. positive regulation of inflammatory response Source: BHF-UCL
  10. positive regulation of interferon-alpha biosynthetic process Source: UniProtKB
  11. positive regulation of interferon-beta biosynthetic process Source: UniProtKB
  12. positive regulation of interferon-gamma biosynthetic process Source: UniProtKB
  13. positive regulation of interleukin-6 production Source: Ensembl
  14. positive regulation of interleukin-8 biosynthetic process Source: UniProtKB
  15. positive regulation of interleukin-8 production Source: BHF-UCL
  16. positive regulation of NF-kappaB import into nucleus Source: BHF-UCL
  17. toll-like receptor 7 signaling pathway Source: Ensembl
  18. toll-like receptor 9 signaling pathway Source: Reactome
  19. toll-like receptor signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Immunity, Inflammatory response, Innate immunity

Enzyme and pathway databases

ReactomeiREACT_118632. Trafficking and processing of endosomal TLR.
REACT_25024. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
REACT_25120. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
REACT_25222. MyD88 dependent cascade initiated on endosome.
REACT_9020. Toll Like Receptor 7/8 (TLR7/8) Cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Toll-like receptor 7
Gene namesi
Name:TLR7
ORF Names:UNQ248/PRO285
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome X

Organism-specific databases

HGNCiHGNC:15631. TLR7.

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity. Endosome By similarity. Lysosome By similarity. Cytoplasmic vesiclephagosome
Note: Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 839813ExtracellularSequence AnalysisAdd
BLAST
Transmembranei840 – 86021HelicalSequence AnalysisAdd
BLAST
Topological domaini861 – 1049189CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: BHF-UCL
  2. early phagosome Source: UniProtKB
  3. endolysosome membrane Source: Reactome
  4. endoplasmic reticulum Source: UniProtKB
  5. endoplasmic reticulum membrane Source: Reactome
  6. endosome Source: UniProtKB
  7. endosome membrane Source: Reactome
  8. Golgi membrane Source: Reactome
  9. integral component of membrane Source: UniProtKB-KW
  10. lysosome Source: UniProtKB
  11. plasma membrane Source: BHF-UCL
  12. receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Lysosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38008.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 10491023Toll-like receptor 7PRO_0000034733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi66 – 661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi167 – 1671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi202 – 2021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi413 – 4131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi488 – 4881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi534 – 5341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi590 – 5901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi679 – 6791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi720 – 7201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi799 – 7991N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9NYK1.
PeptideAtlasiQ9NYK1.
PRIDEiQ9NYK1.

PTM databases

PhosphoSiteiQ9NYK1.

Expressioni

Tissue specificityi

Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells.

Gene expression databases

BgeeiQ9NYK1.
CleanExiHS_TLR7.
ExpressionAtlasiQ9NYK1. baseline and differential.
GenevestigatoriQ9NYK1.

Organism-specific databases

HPAiHPA059613.

Interactioni

Subunit structurei

Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi119436. 2 interactions.
IntActiQ9NYK1. 8 interactions.
STRINGi9606.ENSP00000370034.

Structurei

3D structure databases

ProteinModelPortaliQ9NYK1.
SMRiQ9NYK1. Positions 32-829, 891-1035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati43 – 6422LRR 1Add
BLAST
Repeati65 – 8723LRR 2Add
BLAST
Repeati110 – 12617LRR 3Add
BLAST
Repeati127 – 14923LRR 4Add
BLAST
Repeati151 – 17020LRR 5Add
BLAST
Repeati171 – 19525LRR 6Add
BLAST
Repeati203 – 22624LRR 7Add
BLAST
Repeati228 – 24720LRR 8Add
BLAST
Repeati248 – 27528LRR 9Add
BLAST
Repeati289 – 31224LRR 10Add
BLAST
Repeati314 – 33724LRR 11Add
BLAST
Repeati339 – 36830LRR 12Add
BLAST
Repeati369 – 39224LRR 13Add
BLAST
Repeati396 – 41924LRR 14Add
BLAST
Repeati421 – 44323LRR 15Add
BLAST
Repeati492 – 51524LRR 16Add
BLAST
Repeati516 – 54025LRR 17Add
BLAST
Repeati541 – 56424LRR 18Add
BLAST
Repeati566 – 58823LRR 19Add
BLAST
Repeati595 – 61824LRR 20Add
BLAST
Repeati619 – 64426LRR 21Add
BLAST
Repeati649 – 67224LRR 22Add
BLAST
Repeati674 – 69724LRR 23Add
BLAST
Repeati698 – 72124LRR 24Add
BLAST
Repeati723 – 74523LRR 25Add
BLAST
Repeati746 – 76924LRR 26Add
BLAST
Repeati772 – 79524LRR 27Add
BLAST
Domaini889 – 1036148TIRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Toll-like receptor family.Curated
Contains 27 LRR (leucine-rich) repeats.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG286662.
GeneTreeiENSGT00760000119006.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiQ9NYK1.
KOiK05404.
OMAiAFYLSHQ.
OrthoDBiEOG7VB2DM.
PhylomeDBiQ9NYK1.
TreeFamiTF325595.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR026880. TLR7.
[Graphical view]
PANTHERiPTHR24365:SF222. PTHR24365:SF222. 1 hit.
PfamiPF00560. LRR_1. 3 hits.
PF13306. LRR_5. 1 hit.
PF13855. LRR_8. 2 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 18 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NYK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVFPMWTLKR QILILFNIIL ISKLLGARWF PKTLPCDVTL DVPKNHVIVD
60 70 80 90 100
CTDKHLTEIP GGIPTNTTNL TLTINHIPDI SPASFHRLDH LVEIDFRCNC
110 120 130 140 150
VPIPLGSKNN MCIKRLQIKP RSFSGLTYLK SLYLDGNQLL EIPQGLPPSL
160 170 180 190 200
QLLSLEANNI FSIRKENLTE LANIEILYLG QNCYYRNPCY VSYSIEKDAF
210 220 230 240 250
LNLTKLKVLS LKDNNVTAVP TVLPSTLTEL YLYNNMIAKI QEDDFNNLNQ
260 270 280 290 300
LQILDLSGNC PRCYNAPFPC APCKNNSPLQ IPVNAFDALT ELKVLRLHSN
310 320 330 340 350
SLQHVPPRWF KNINKLQELD LSQNFLAKEI GDAKFLHFLP SLIQLDLSFN
360 370 380 390 400
FELQVYRASM NLSQAFSSLK SLKILRIRGY VFKELKSFNL SPLHNLQNLE
410 420 430 440 450
VLDLGTNFIK IANLSMFKQF KRLKVIDLSV NKISPSGDSS EVGFCSNART
460 470 480 490 500
SVESYEPQVL EQLHYFRYDK YARSCRFKNK EASFMSVNES CYKYGQTLDL
510 520 530 540 550
SKNSIFFVKS SDFQHLSFLK CLNLSGNLIS QTLNGSEFQP LAELRYLDFS
560 570 580 590 600
NNRLDLLHST AFEELHKLEV LDISSNSHYF QSEGITHMLN FTKNLKVLQK
610 620 630 640 650
LMMNDNDISS STSRTMESES LRTLEFRGNH LDVLWREGDN RYLQLFKNLL
660 670 680 690 700
KLEELDISKN SLSFLPSGVF DGMPPNLKNL SLAKNGLKSF SWKKLQCLKN
710 720 730 740 750
LETLDLSHNQ LTTVPERLSN CSRSLKNLIL KNNQIRSLTK YFLQDAFQLR
760 770 780 790 800
YLDLSSNKIQ MIQKTSFPEN VLNNLKMLLL HHNRFLCTCD AVWFVWWVNH
810 820 830 840 850
TEVTIPYLAT DVTCVGPGAH KGQSVISLDL YTCELDLTNL ILFSLSISVS
860 870 880 890 900
LFLMVMMTAS HLYFWDVWYI YHFCKAKIKG YQRLISPDCC YDAFIVYDTK
910 920 930 940 950
DPAVTEWVLA ELVAKLEDPR EKHFNLCLEE RDWLPGQPVL ENLSQSIQLS
960 970 980 990 1000
KKTVFVMTDK YAKTENFKIA FYLSHQRLMD EKVDVIILIF LEKPFQKSKF
1010 1020 1030 1040
LQLRKRLCGS SVLEWPTNPQ AHPYFWQCLK NALATDNHVA YSQVFKETV
Length:1,049
Mass (Da):120,922
Last modified:October 1, 2000 - v1
Checksum:i8C701E9E437F2721
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti725 – 7251L → H in AAF78035 (PubMed:11022120).Curated
Sequence conflicti738 – 7381L → P in AAF78035 (PubMed:11022120).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti11 – 111Q → L.1 Publication
Corresponds to variant rs179008 [ dbSNP | Ensembl ].
VAR_034554
Natural varianti448 – 4481A → V.
Corresponds to variant rs5743781 [ dbSNP | Ensembl ].
VAR_024665

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240467 mRNA. Translation: AAF60188.1.
AF245702 mRNA. Translation: AAF78035.1.
DQ022185 Genomic DNA. Translation: AAZ99026.1.
AY358292 mRNA. Translation: AAQ88659.1.
BC033651 mRNA. Translation: AAH33651.1.
CCDSiCCDS14151.1.
RefSeqiNP_057646.1. NM_016562.3.
UniGeneiHs.659215.

Genome annotation databases

EnsembliENST00000380659; ENSP00000370034; ENSG00000196664.
GeneIDi51284.
KEGGihsa:51284.
UCSCiuc004cvc.3. human.

Polymorphism databases

DMDMi20140876.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240467 mRNA. Translation: AAF60188.1.
AF245702 mRNA. Translation: AAF78035.1.
DQ022185 Genomic DNA. Translation: AAZ99026.1.
AY358292 mRNA. Translation: AAQ88659.1.
BC033651 mRNA. Translation: AAH33651.1.
CCDSiCCDS14151.1.
RefSeqiNP_057646.1. NM_016562.3.
UniGeneiHs.659215.

3D structure databases

ProteinModelPortaliQ9NYK1.
SMRiQ9NYK1. Positions 32-829, 891-1035.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119436. 2 interactions.
IntActiQ9NYK1. 8 interactions.
STRINGi9606.ENSP00000370034.

Chemistry

BindingDBiQ9NYK1.
ChEMBLiCHEMBL3137288.
DrugBankiDB01611. Hydroxychloroquine.
DB00724. Imiquimod.
GuidetoPHARMACOLOGYi1757.

PTM databases

PhosphoSiteiQ9NYK1.

Polymorphism databases

DMDMi20140876.

Proteomic databases

PaxDbiQ9NYK1.
PeptideAtlasiQ9NYK1.
PRIDEiQ9NYK1.

Protocols and materials databases

DNASUi51284.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380659; ENSP00000370034; ENSG00000196664.
GeneIDi51284.
KEGGihsa:51284.
UCSCiuc004cvc.3. human.

Organism-specific databases

CTDi51284.
GeneCardsiGC0XP012885.
HGNCiHGNC:15631. TLR7.
HPAiHPA059613.
MIMi300365. gene.
neXtProtiNX_Q9NYK1.
PharmGKBiPA38008.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG286662.
GeneTreeiENSGT00760000119006.
HOGENOMiHOG000230468.
HOVERGENiHBG018601.
InParanoidiQ9NYK1.
KOiK05404.
OMAiAFYLSHQ.
OrthoDBiEOG7VB2DM.
PhylomeDBiQ9NYK1.
TreeFamiTF325595.

Enzyme and pathway databases

ReactomeiREACT_118632. Trafficking and processing of endosomal TLR.
REACT_25024. TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation.
REACT_25120. TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling.
REACT_25222. MyD88 dependent cascade initiated on endosome.
REACT_9020. Toll Like Receptor 7/8 (TLR7/8) Cascade.

Miscellaneous databases

ChiTaRSiTLR7. human.
GeneWikiiTLR_7.
GenomeRNAii51284.
NextBioi35489043.
PROiQ9NYK1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NYK1.
CleanExiHS_TLR7.
ExpressionAtlasiQ9NYK1. baseline and differential.
GenevestigatoriQ9NYK1.

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
IPR026906. LRR_5.
IPR000157. TIR_dom.
IPR026880. TLR7.
[Graphical view]
PANTHERiPTHR24365:SF222. PTHR24365:SF222. 1 hit.
PfamiPF00560. LRR_1. 3 hits.
PF13306. LRR_5. 1 hit.
PF13855. LRR_8. 2 hits.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52200. SSF52200. 1 hit.
PROSITEiPS51450. LRR. 18 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three novel mammalian Toll-like receptors: gene structure, expression, and evolution."
    Du X., Poltorak A., Wei Y., Beutler B.
    Eur. Cytokine Netw. 11:362-371(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Cloning and characterization of a sub-family of human Toll-like receptors: hTLR7, hTLR8 and hTLR9."
    Chuang T.-H., Ulevitch R.J.
    Eur. Cytokine Netw. 11:372-378(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  3. "The heterogeneous allelic repertoire of human Toll-Like receptor (TLR) genes."
    Georgel P., Macquin C., Bahram S.
    PLoS ONE 4:E7803-E7803(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LEU-11.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  6. "TLR7 is involved in sequence-specific sensing of single-stranded RNAs in human macrophages."
    Gantier M.P., Tong S., Behlke M.A., Xu D., Phipps S., Foster P.S., Williams B.R.
    J. Immunol. 180:2117-2124(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTLR7_HUMAN
AccessioniPrimary (citable) accession number: Q9NYK1
Secondary accession number(s): D1CS69, Q9NR98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.