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Q9NYG8

- KCNK4_HUMAN

UniProt

Q9NYG8 - KCNK4_HUMAN

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Protein

Potassium channel subfamily K member 4

Gene

KCNK4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Voltage insensitive, instantaneous, outwardly rectifying potassium channel. Outward rectification is reversed at high external K+ concentrations (By similarity).By similarity

GO - Molecular functioni

  1. potassium channel activity Source: ProtInc
  2. voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

  1. potassium ion transport Source: ProtInc
  2. synaptic transmission Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_75819. TWIK related potassium channel (TREK).

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel subfamily K member 4
Alternative name(s):
TWIK-related arachidonic acid-stimulated potassium channel protein
Short name:
TRAAK
Two pore potassium channel KT4.1
Short name:
Two pore K(+) channel KT4.1
Gene namesi
Name:KCNK4
Synonyms:TRAAK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:6279. KCNK4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 33CytoplasmicSequence Analysis
Transmembranei4 – 2421HelicalSequence AnalysisAdd
BLAST
Intramembranei89 – 11325Pore-forming; Name=Pore-forming 1Sequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Topological domaini139 – 17133CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST
Intramembranei197 – 22125Pore-forming; Name=Pore-forming 2Sequence AnalysisAdd
BLAST
Transmembranei234 – 25421HelicalSequence AnalysisAdd
BLAST
Topological domaini255 – 393139CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30061.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Potassium channel subfamily K member 4PRO_0000101747Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 – 52Interchain1 Publication
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NYG8.
PRIDEiQ9NYG8.

PTM databases

PhosphoSiteiQ9NYG8.

Expressioni

Gene expression databases

BgeeiQ9NYG8.
CleanExiHS_KCNK4.
ExpressionAtlasiQ9NYG8. baseline.
GenevestigatoriQ9NYG8.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.1 Publication

Protein-protein interaction databases

BioGridi119122. 2 interactions.
IntActiQ9NYG8. 2 interactions.
STRINGi9606.ENSP00000378033.

Structurei

Secondary structure

1
393
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2826Combined sources
Helixi29 – 313Combined sources
Helixi34 – 4916Combined sources
Helixi55 – 7016Combined sources
Helixi90 – 10112Combined sources
Helixi114 – 15946Combined sources
Helixi164 – 18320Combined sources
Helixi185 – 19511Combined sources
Helixi199 – 21012Combined sources
Beta strandi216 – 2183Combined sources
Helixi231 – 25929Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UM7X-ray3.31A/B1-274[»]
4I9WX-ray2.75A/B1-274[»]
ProteinModelPortaliQ9NYG8.
SMRiQ9NYG8. Positions 2-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG280717.
GeneTreeiENSGT00760000118858.
HOGENOMiHOG000013106.
HOVERGENiHBG052234.
InParanoidiQ9NYG8.
KOiK04915.
OMAiAYQPLVW.
PhylomeDBiQ9NYG8.
TreeFamiTF313947.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR008074. 2pore_dom_K_chnl_TRAAK.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9NYG8-1) [UniParc]FASTAAdd to Basket

Also known as: KT4.1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSTTLLALL ALVLLYLVSG ALVFRALEQP HEQQAQRELG EVREKFLRAH
60 70 80 90 100
PCVSDQELGL LIKEVADALG GGADPETNST SNSSHSAWDL GSAFFFSGTI
110 120 130 140 150
ITTIGYGNVA LRTDAGRLFC IFYALVGIPL FGILLAGVGD RLGSSLRHGI
160 170 180 190 200
GHIEAIFLKW HVPPELVRVL SAMLFLLIGC LLFVLTPTFV FCYMEDWSKL
210 220 230 240 250
EAIYFVIVTL TTVGFGDYVA GADPRQDSPA YQPLVWFWIL LGLAYFASVL
260 270 280 290 300
TTIGNWLRVV SRRTRAEMGG LTAQAASWTG TVTARVTQRA GPAAPPPEKE
310 320 330 340 350
QPLLPPPPCP AQPLGRPRSP SPPEKAQPPS PPTASALDYP SENLAFIDES
360 370 380 390
SDTQSERGCP LPRAPRGRRR PNPPRKPVRP RGPGRPRDKG VPV
Length:393
Mass (Da):42,704
Last modified:February 21, 2001 - v2
Checksum:i7F18E53A0A9AD57D
GO
Isoform 2 (identifier: Q9NYG8-2) [UniParc]FASTAAdd to Basket

Also known as: KT4.1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTTAPQEPPARPLQAGSGAGPAPGRAM

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Show »
Length:419
Mass (Da):45,173
Checksum:iE7DEDE17B30C0FBF
GO

Sequence cautioni

The sequence AAF64062.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti328 – 3281P → L.1 Publication
Corresponds to variant rs953778 [ dbSNP | Ensembl ].
VAR_020206

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MTTAPQEPPARPLQAGSGAG PAPGRAM in isoform 2. 1 PublicationVSP_006689

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248242 mRNA. Translation: AAG31731.1.
AF247042 mRNA. Translation: AAF64062.1. Different initiation.
AF259500 mRNA. Translation: AAK49389.1.
AF259501 mRNA. Translation: AAK49390.1.
EU978935 mRNA. Translation: ACH86094.1.
CH471076 Genomic DNA. Translation: EAW74242.1.
CCDSiCCDS8067.1. [Q9NYG8-1]
RefSeqiNP_201567.1. NM_033310.2. [Q9NYG8-1]
UniGeneiHs.647233.

Genome annotation databases

EnsembliENST00000394525; ENSP00000378033; ENSG00000182450. [Q9NYG8-1]
ENST00000422670; ENSP00000402797; ENSG00000182450. [Q9NYG8-1]
ENST00000539216; ENSP00000444948; ENSG00000182450. [Q9NYG8-1]
GeneIDi50801.
KEGGihsa:50801.
UCSCiuc001nzj.1. human. [Q9NYG8-1]

Polymorphism databases

DMDMi13124080.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF248242 mRNA. Translation: AAG31731.1 .
AF247042 mRNA. Translation: AAF64062.1 . Different initiation.
AF259500 mRNA. Translation: AAK49389.1 .
AF259501 mRNA. Translation: AAK49390.1 .
EU978935 mRNA. Translation: ACH86094.1 .
CH471076 Genomic DNA. Translation: EAW74242.1 .
CCDSi CCDS8067.1. [Q9NYG8-1 ]
RefSeqi NP_201567.1. NM_033310.2. [Q9NYG8-1 ]
UniGenei Hs.647233.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3UM7 X-ray 3.31 A/B 1-274 [» ]
4I9W X-ray 2.75 A/B 1-274 [» ]
ProteinModelPortali Q9NYG8.
SMRi Q9NYG8. Positions 2-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119122. 2 interactions.
IntActi Q9NYG8. 2 interactions.
STRINGi 9606.ENSP00000378033.

Chemistry

GuidetoPHARMACOLOGYi 516.

PTM databases

PhosphoSitei Q9NYG8.

Polymorphism databases

DMDMi 13124080.

Proteomic databases

PaxDbi Q9NYG8.
PRIDEi Q9NYG8.

Protocols and materials databases

DNASUi 50801.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000394525 ; ENSP00000378033 ; ENSG00000182450 . [Q9NYG8-1 ]
ENST00000422670 ; ENSP00000402797 ; ENSG00000182450 . [Q9NYG8-1 ]
ENST00000539216 ; ENSP00000444948 ; ENSG00000182450 . [Q9NYG8-1 ]
GeneIDi 50801.
KEGGi hsa:50801.
UCSCi uc001nzj.1. human. [Q9NYG8-1 ]

Organism-specific databases

CTDi 50801.
GeneCardsi GC11P064058.
HGNCi HGNC:6279. KCNK4.
MIMi 605720. gene.
neXtProti NX_Q9NYG8.
PharmGKBi PA30061.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG280717.
GeneTreei ENSGT00760000118858.
HOGENOMi HOG000013106.
HOVERGENi HBG052234.
InParanoidi Q9NYG8.
KOi K04915.
OMAi AYQPLVW.
PhylomeDBi Q9NYG8.
TreeFami TF313947.

Enzyme and pathway databases

Reactomei REACT_75819. TWIK related potassium channel (TREK).

Miscellaneous databases

GeneWikii KCNK4.
GenomeRNAii 50801.
NextBioi 53228.
PROi Q9NYG8.
SOURCEi Search...

Gene expression databases

Bgeei Q9NYG8.
CleanExi HS_KCNK4.
ExpressionAtlasi Q9NYG8. baseline.
Genevestigatori Q9NYG8.

Family and domain databases

InterProi IPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR008074. 2pore_dom_K_chnl_TRAAK.
[Graphical view ]
Pfami PF07885. Ion_trans_2. 2 hits.
[Graphical view ]
PRINTSi PR01333. 2POREKCHANEL.
PR01691. TRAAKCHANNEL.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, localisation and functional expression of a novel human, cerebellum specific, two pore domain potassium channel."
    Chapman C.G., Meadows H.J., Godden R.J., Campbell D.A., Duckworth M., Kelsell R.E., Murdock P.R., Randall A.D., Rennie G.I., Gloger I.S.
    Brain Res. Mol. Brain Res. 82:74-83(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Assignment of KCNK4 encoding the human potassium channel TRAAK to chromosome 11."
    Gray A.T.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-328.
    Tissue: Frontal cortex.
  3. "Cloning and expression of human TRAAK, a polyunsaturated fatty acids-activated and mechano-sensitive K(+) channel."
    Lesage F., Maingret F., Lazdunski M.
    FEBS Lett. 471:137-140(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Cloning of two transcripts, HKT4.1a and HKT4.1b, from the human two-pore K+ channel gene KCNK4. Chromosomal localization, tissue distribution and functional expression."
    Ozaita A., Vega-Saenz de Miera E.
    Brain Res. Mol. Brain Res. 102:18-27(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1).
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
  8. "Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel."
    Brohawn S.G., del Marmol J., MacKinnon R.
    Science 335:436-441(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.31 ANGSTROMS) OF 1-274, DISULFIDE BOND.

Entry informationi

Entry nameiKCNK4_HUMAN
AccessioniPrimary (citable) accession number: Q9NYG8
Secondary accession number(s): B5TJL1, Q96T94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 26, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3