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Q9NYG8

- KCNK4_HUMAN

UniProt

Q9NYG8 - KCNK4_HUMAN

Protein

Potassium channel subfamily K member 4

Gene

KCNK4

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (21 Feb 2001)
      Previous versions | rss
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    Functioni

    Voltage insensitive, instantaneous, outwardly rectifying potassium channel. Outward rectification is reversed at high external K+ concentrations By similarity.By similarity

    GO - Molecular functioni

    1. potassium channel activity Source: ProtInc
    2. voltage-gated ion channel activity Source: UniProtKB-KW

    GO - Biological processi

    1. potassium ion transport Source: ProtInc
    2. synaptic transmission Source: Reactome

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_75819. TWIK related potassium channel (TREK).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel subfamily K member 4
    Alternative name(s):
    TWIK-related arachidonic acid-stimulated potassium channel protein
    Short name:
    TRAAK
    Two pore potassium channel KT4.1
    Short name:
    Two pore K(+) channel KT4.1
    Gene namesi
    Name:KCNK4
    Synonyms:TRAAK
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:6279. KCNK4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA30061.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393Potassium channel subfamily K member 4PRO_0000101747Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi52 – 52Interchain1 Publication
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9NYG8.
    PRIDEiQ9NYG8.

    PTM databases

    PhosphoSiteiQ9NYG8.

    Expressioni

    Gene expression databases

    BgeeiQ9NYG8.
    CleanExiHS_KCNK4.
    GenevestigatoriQ9NYG8.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.1 Publication

    Protein-protein interaction databases

    BioGridi119122. 2 interactions.
    IntActiQ9NYG8. 2 interactions.
    STRINGi9606.ENSP00000378033.

    Structurei

    Secondary structure

    1
    393
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 2826
    Helixi29 – 313
    Helixi34 – 4916
    Helixi55 – 7016
    Helixi90 – 10112
    Helixi114 – 15946
    Helixi164 – 18320
    Helixi185 – 19511
    Helixi199 – 21012
    Beta strandi216 – 2183
    Helixi231 – 25929

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3UM7X-ray3.31A/B1-274[»]
    4I9WX-ray2.75A/B1-274[»]
    ProteinModelPortaliQ9NYG8.
    SMRiQ9NYG8. Positions 2-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 33CytoplasmicSequence Analysis
    Topological domaini139 – 17133CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini255 – 393139CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei89 – 11325Pore-forming; Name=Pore-forming 1Sequence AnalysisAdd
    BLAST
    Intramembranei197 – 22125Pore-forming; Name=Pore-forming 2Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei234 – 25421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG280717.
    HOGENOMiHOG000013106.
    HOVERGENiHBG052234.
    InParanoidiQ9NYG8.
    KOiK04915.
    OMAiAYQPLVW.
    PhylomeDBiQ9NYG8.
    TreeFamiTF313947.

    Family and domain databases

    InterProiIPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR008074. 2pore_dom_K_chnl_TRAAK.
    [Graphical view]
    PfamiPF07885. Ion_trans_2. 2 hits.
    [Graphical view]
    PRINTSiPR01333. 2POREKCHANEL.
    PR01691. TRAAKCHANNEL.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NYG8-1) [UniParc]FASTAAdd to Basket

    Also known as: KT4.1a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRSTTLLALL ALVLLYLVSG ALVFRALEQP HEQQAQRELG EVREKFLRAH    50
    PCVSDQELGL LIKEVADALG GGADPETNST SNSSHSAWDL GSAFFFSGTI 100
    ITTIGYGNVA LRTDAGRLFC IFYALVGIPL FGILLAGVGD RLGSSLRHGI 150
    GHIEAIFLKW HVPPELVRVL SAMLFLLIGC LLFVLTPTFV FCYMEDWSKL 200
    EAIYFVIVTL TTVGFGDYVA GADPRQDSPA YQPLVWFWIL LGLAYFASVL 250
    TTIGNWLRVV SRRTRAEMGG LTAQAASWTG TVTARVTQRA GPAAPPPEKE 300
    QPLLPPPPCP AQPLGRPRSP SPPEKAQPPS PPTASALDYP SENLAFIDES 350
    SDTQSERGCP LPRAPRGRRR PNPPRKPVRP RGPGRPRDKG VPV 393
    Length:393
    Mass (Da):42,704
    Last modified:February 21, 2001 - v2
    Checksum:i7F18E53A0A9AD57D
    GO
    Isoform 2 (identifier: Q9NYG8-2) [UniParc]FASTAAdd to Basket

    Also known as: KT4.1b

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MTTAPQEPPARPLQAGSGAGPAPGRAM

    Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Show »
    Length:419
    Mass (Da):45,173
    Checksum:iE7DEDE17B30C0FBF
    GO

    Sequence cautioni

    The sequence AAF64062.1 differs from that shown. Reason: Erroneous initiation.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti328 – 3281P → L.1 Publication
    Corresponds to variant rs953778 [ dbSNP | Ensembl ].
    VAR_020206

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MTTAPQEPPARPLQAGSGAG PAPGRAM in isoform 2. 1 PublicationVSP_006689

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF248242 mRNA. Translation: AAG31731.1.
    AF247042 mRNA. Translation: AAF64062.1. Different initiation.
    AF259500 mRNA. Translation: AAK49389.1.
    AF259501 mRNA. Translation: AAK49390.1.
    EU978935 mRNA. Translation: ACH86094.1.
    CH471076 Genomic DNA. Translation: EAW74242.1.
    CCDSiCCDS8067.1. [Q9NYG8-1]
    RefSeqiNP_201567.1. NM_033310.2. [Q9NYG8-1]
    UniGeneiHs.647233.

    Genome annotation databases

    EnsembliENST00000394525; ENSP00000378033; ENSG00000182450. [Q9NYG8-1]
    ENST00000422670; ENSP00000402797; ENSG00000182450. [Q9NYG8-1]
    ENST00000539216; ENSP00000444948; ENSG00000182450. [Q9NYG8-1]
    GeneIDi50801.
    KEGGihsa:50801.
    UCSCiuc001nzj.1. human. [Q9NYG8-1]

    Polymorphism databases

    DMDMi13124080.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF248242 mRNA. Translation: AAG31731.1 .
    AF247042 mRNA. Translation: AAF64062.1 . Different initiation.
    AF259500 mRNA. Translation: AAK49389.1 .
    AF259501 mRNA. Translation: AAK49390.1 .
    EU978935 mRNA. Translation: ACH86094.1 .
    CH471076 Genomic DNA. Translation: EAW74242.1 .
    CCDSi CCDS8067.1. [Q9NYG8-1 ]
    RefSeqi NP_201567.1. NM_033310.2. [Q9NYG8-1 ]
    UniGenei Hs.647233.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3UM7 X-ray 3.31 A/B 1-274 [» ]
    4I9W X-ray 2.75 A/B 1-274 [» ]
    ProteinModelPortali Q9NYG8.
    SMRi Q9NYG8. Positions 2-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119122. 2 interactions.
    IntActi Q9NYG8. 2 interactions.
    STRINGi 9606.ENSP00000378033.

    Chemistry

    GuidetoPHARMACOLOGYi 516.

    PTM databases

    PhosphoSitei Q9NYG8.

    Polymorphism databases

    DMDMi 13124080.

    Proteomic databases

    PaxDbi Q9NYG8.
    PRIDEi Q9NYG8.

    Protocols and materials databases

    DNASUi 50801.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000394525 ; ENSP00000378033 ; ENSG00000182450 . [Q9NYG8-1 ]
    ENST00000422670 ; ENSP00000402797 ; ENSG00000182450 . [Q9NYG8-1 ]
    ENST00000539216 ; ENSP00000444948 ; ENSG00000182450 . [Q9NYG8-1 ]
    GeneIDi 50801.
    KEGGi hsa:50801.
    UCSCi uc001nzj.1. human. [Q9NYG8-1 ]

    Organism-specific databases

    CTDi 50801.
    GeneCardsi GC11P064058.
    HGNCi HGNC:6279. KCNK4.
    MIMi 605720. gene.
    neXtProti NX_Q9NYG8.
    PharmGKBi PA30061.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG280717.
    HOGENOMi HOG000013106.
    HOVERGENi HBG052234.
    InParanoidi Q9NYG8.
    KOi K04915.
    OMAi AYQPLVW.
    PhylomeDBi Q9NYG8.
    TreeFami TF313947.

    Enzyme and pathway databases

    Reactomei REACT_75819. TWIK related potassium channel (TREK).

    Miscellaneous databases

    GeneWikii KCNK4.
    GenomeRNAii 50801.
    NextBioi 53228.
    PROi Q9NYG8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9NYG8.
    CleanExi HS_KCNK4.
    Genevestigatori Q9NYG8.

    Family and domain databases

    InterProi IPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR008074. 2pore_dom_K_chnl_TRAAK.
    [Graphical view ]
    Pfami PF07885. Ion_trans_2. 2 hits.
    [Graphical view ]
    PRINTSi PR01333. 2POREKCHANEL.
    PR01691. TRAAKCHANNEL.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, localisation and functional expression of a novel human, cerebellum specific, two pore domain potassium channel."
      Chapman C.G., Meadows H.J., Godden R.J., Campbell D.A., Duckworth M., Kelsell R.E., Murdock P.R., Randall A.D., Rennie G.I., Gloger I.S.
      Brain Res. Mol. Brain Res. 82:74-83(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "Assignment of KCNK4 encoding the human potassium channel TRAAK to chromosome 11."
      Gray A.T.
      Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT LEU-328.
      Tissue: Frontal cortex.
    3. "Cloning and expression of human TRAAK, a polyunsaturated fatty acids-activated and mechano-sensitive K(+) channel."
      Lesage F., Maingret F., Lazdunski M.
      FEBS Lett. 471:137-140(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Cloning of two transcripts, HKT4.1a and HKT4.1b, from the human two-pore K+ channel gene KCNK4. Chromosomal localization, tissue distribution and functional expression."
      Ozaita A., Vega-Saenz de Miera E.
      Brain Res. Mol. Brain Res. 102:18-27(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 1).
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
    8. "Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel."
      Brohawn S.G., del Marmol J., MacKinnon R.
      Science 335:436-441(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.31 ANGSTROMS) OF 1-274, DISULFIDE BOND.

    Entry informationi

    Entry nameiKCNK4_HUMAN
    AccessioniPrimary (citable) accession number: Q9NYG8
    Secondary accession number(s): B5TJL1, Q96T94
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: February 21, 2001
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3