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Protein

N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2

Gene

B3GNT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Shows a marked preference for Gal(beta1-4)Glc(NAc)-based acceptors (PubMed:9892646). Probably constitutes the main polylactosamine synthase.3 Publications

Caution

Was indicated as B3Gal-T6 in submitted DNA entries.Curated

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R.3 Publications

Cofactori

Mn2+1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese

Enzyme and pathway databases

BioCyciMetaCyc:ENSG00000170340-MONOMER
ReactomeiR-HSA-2022854 Keratan sulfate biosynthesis
R-HSA-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2 (EC:2.4.1.1493 Publications)
Alternative name(s):
Beta-1,3-N-acetylglucosaminyltransferase 1
Short name:
BGnT-1
Short name:
Beta-1,3-Gn-T1
Short name:
Beta3Gn-T1
Beta-1,3-galactosyltransferase 7
Short name:
Beta-1,3-GalTase 7
Short name:
Beta3Gal-T7
Short name:
Beta3GalT7
Short name:
b3Gal-T7
Beta-3-Gx-T7
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Short name:
BGnT-2
Short name:
Beta-1,3-Gn-T2
Short name:
Beta-1,3-N-acetylglucosaminyltransferase 2
Short name:
Beta3Gn-T2
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7
Gene namesi
Name:B3GNT2
Synonyms:B3GALT7, B3GNT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000170340.10
HGNCiHGNC:15629 B3GNT2
MIMi605581 gene
neXtProtiNX_Q9NY97

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 397LumenalSequence analysisAdd BLAST369

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi245D → A: Loss of enzymatic activity, no loss of B3GNT8-binding. 1 Publication1

Organism-specific databases

DisGeNETi10678
OpenTargetsiENSG00000170340
PharmGKBiPA25218

Polymorphism and mutation databases

BioMutaiB3GNT2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191701 – 397N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 2Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi79N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi127N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9NY97
MaxQBiQ9NY97
PaxDbiQ9NY97
PeptideAtlasiQ9NY97
PRIDEiQ9NY97

PTM databases

iPTMnetiQ9NY97
PhosphoSitePlusiQ9NY97

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSG00000170340
CleanExiHS_B3GNT1
HS_B3GNT2
GenevisibleiQ9NY97 HS

Organism-specific databases

HPAiHPA005997

Interactioni

Subunit structurei

Interacts with B3GNT8; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.1 Publication

Protein-protein interaction databases

BioGridi11591937 interactors.
IntActiQ9NY97 1 interactor.
STRINGi9606.ENSP00000305595

Structurei

3D structure databases

ProteinModelPortaliQ9NY97
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2287 Eukaryota
ENOG410ZZ1B LUCA
GeneTreeiENSGT00760000118879
HOGENOMiHOG000232195
HOVERGENiHBG050653
InParanoidiQ9NY97
KOiK00741
OMAiMDQPDIC
OrthoDBiEOG091G0AXM
PhylomeDBiQ9NY97
TreeFamiTF318639

Family and domain databases

InterProiView protein in InterPro
IPR002659 Glyco_trans_31
PANTHERiPTHR11214 PTHR11214, 1 hit
PfamiView protein in Pfam
PF01762 Galactosyl_T, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NY97-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVGRRRIKL LGILMMANVF IYFIMEVSKS SSQEKNGKGE VIIPKEKFWK
60 70 80 90 100
ISTPPEAYWN REQEKLNRQY NPILSMLTNQ TGEAGRLSNI SHLNYCEPDL
110 120 130 140 150
RVTSVVTGFN NLPDRFKDFL LYLRCRNYSL LIDQPDKCAK KPFLLLAIKS
160 170 180 190 200
LTPHFARRQA IRESWGQESN AGNQTVVRVF LLGQTPPEDN HPDLSDMLKF
210 220 230 240 250
ESEKHQDILM WNYRDTFFNL SLKEVLFLRW VSTSCPDTEF VFKGDDDVFV
260 270 280 290 300
NTHHILNYLN SLSKTKAKDL FIGDVIHNAG PHRDKKLKYY IPEVVYSGLY
310 320 330 340 350
PPYAGGGGFL YSGHLALRLY HITDQVHLYP IDDVYTGMCL QKLGLVPEKH
360 370 380 390
KGFRTFDIEE KNKNNICSYV DLMLVHSRKP QEMIDIWSQL QSAHLKC
Length:397
Mass (Da):46,022
Last modified:April 11, 2003 - v2
Checksum:iB104ECCAE26DC4AC
GO
Isoform 2 (identifier: Q9NY97-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MSVGRRRIKLL → MVSRSLV

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:393
Mass (Da):45,484
Checksum:iDA6ECD0416167FEF
GO

Sequence cautioni

The sequence BAA92031 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11L → LL in CAB91546 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0017911 – 11MSVGRRRIKLL → MVSRSLV in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF092051 mRNA Translation: AAD09764.2
AJ006077 mRNA Translation: CAB91546.1
AF288208 mRNA Translation: AAF97253.1
AF288209 mRNA Translation: AAF97254.1
AB049584 mRNA Translation: BAB21530.1
BC030579 mRNA Translation: AAH30579.1
AC093401 Genomic DNA Translation: AAX93271.1
CH471053 Genomic DNA Translation: EAW99977.1
CH471053 Genomic DNA Translation: EAW99978.1
CH471053 Genomic DNA Translation: EAW99979.1
BC047933 mRNA Translation: AAH47933.1
AK002009 mRNA Translation: BAA92031.1 Different initiation.
CCDSiCCDS1870.1 [Q9NY97-1]
RefSeqiNP_001306004.1, NM_001319075.1 [Q9NY97-1]
NP_006568.2, NM_006577.5 [Q9NY97-1]
UniGeneiHs.173203

Genome annotation databases

EnsembliENST00000301998; ENSP00000305595; ENSG00000170340 [Q9NY97-1]
ENST00000405767; ENSP00000384692; ENSG00000170340 [Q9NY97-1]
GeneIDi10678
KEGGihsa:10678
UCSCiuc002sbs.4 human [Q9NY97-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiB3GN2_HUMAN
AccessioniPrimary (citable) accession number: Q9NY97
Secondary accession number(s): Q54AC1
, Q9NQQ9, Q9NQR0, Q9NUT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: April 11, 2003
Last modified: February 28, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome