Q9NY97 (B3GN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Short name=BGnT-2
Short name=Beta-1,3-Gn-T2
Short name=Beta-1,3-N-acetylglucosaminyltransferase 2
Short name=Beta3Gn-T2
EC=2.4.1.-

Alternative name(s):
Beta-1,3-N-acetylglucosaminyltransferase 1
Short name=BGnT-1
Short name=Beta-1,3-Gn-T1
Short name=Beta3Gn-T1
Beta-1,3-galactosyltransferase 7
Short name=Beta-1,3-GalTase 7
Short name=Beta3Gal-T7
Short name=Beta3GalT7
Short name=b3Gal-T7
Beta-3-Gx-T7
UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7
UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7

Gene names

Name:	B3GNT2
Synonyms:	B3GALT7, B3GNT1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the initiation and elongation of poly-N-acetyllactosamine chains. Ref.1
Cofactor	Manganese. Ref.1
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the glycosyltransferase 31 family.
Caution	Was indicated as B3Gal-T6 in submitted DNA entries.
Sequence caution	The sequence BAA92031.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement. Source: UniProtKB
Molecular function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9NY97-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9NY97-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MSVGRRRIKLL → MVSRSLV

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2		PRO_0000219170
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 397	369	Lumenal Potential
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Ref.12
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 11	11	MSVGRRRIKLL → MVSRSLV in isoform 2.		VSP_001791
Experimental info
	Sequence conflict	11	1	L → LL in CAB91546. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 11, 2003. Version 2. Checksum: B104ECCAE26DC4AC	FASTA	397	46,022
	10 20 30 40 50 60 MSVGRRRIKL LGILMMANVF IYFIMEVSKS SSQEKNGKGE VIIPKEKFWK ISTPPEAYWN 70 80 90 100 110 120 REQEKLNRQY NPILSMLTNQ TGEAGRLSNI SHLNYCEPDL RVTSVVTGFN NLPDRFKDFL 130 140 150 160 170 180 LYLRCRNYSL LIDQPDKCAK KPFLLLAIKS LTPHFARRQA IRESWGQESN AGNQTVVRVF 190 200 210 220 230 240 LLGQTPPEDN HPDLSDMLKF ESEKHQDILM WNYRDTFFNL SLKEVLFLRW VSTSCPDTEF 250 260 270 280 290 300 VFKGDDDVFV NTHHILNYLN SLSKTKAKDL FIGDVIHNAG PHRDKKLKYY IPEVVYSGLY 310 320 330 340 350 360 PPYAGGGGFL YSGHLALRLY HITDQVHLYP IDDVYTGMCL QKLGLVPEKH KGFRTFDIEE 370 380 390 KNKNNICSYV DLMLVHSRKP QEMIDIWSQL QSAHLKC
	Isoform 2 [UniParc]. Checksum: DA6ECD0416167FEF	FASTA	393	45,484
	10 20 30 40 50 60 MVSRSLVGIL MMANVFIYFI MEVSKSSSQE KNGKGEVIIP KEKFWKISTP PEAYWNREQE 70 80 90 100 110 120 KLNRQYNPIL SMLTNQTGEA GRLSNISHLN YCEPDLRVTS VVTGFNNLPD RFKDFLLYLR 130 140 150 160 170 180 CRNYSLLIDQ PDKCAKKPFL LLAIKSLTPH FARRQAIRES WGQESNAGNQ TVVRVFLLGQ 190 200 210 220 230 240 TPPEDNHPDL SDMLKFESEK HQDILMWNYR DTFFNLSLKE VLFLRWVSTS CPDTEFVFKG 250 260 270 280 290 300 DDDVFVNTHH ILNYLNSLSK TKAKDLFIGD VIHNAGPHRD KKLKYYIPEV VYSGLYPPYA 310 320 330 340 350 360 GGGGFLYSGH LALRLYHITD QVHLYPIDDV YTGMCLQKLG LVPEKHKGFR TFDIEEKNKN 370 380 390 NICSYVDLML VHSRKPQEMI DIWSQLQSAH LKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta-1,3-galactosyltransferases." Zhou D., Dinter A., Gutierrez Gallego R., Kamerling J.P., Vliegenthart J.F.G., Berger E.G., Hennet T. Proc. Natl. Acad. Sci. U.S.A. 96:406-411(1999) [PubMed: 9892646] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, COFACTOR. Tissue: Brain.
[2]	Zhou D., Berger E.G., Hennet T. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Cloning and expression of two beta-1,3-galactosyltransferases: beta3gal-T5 and beta3gal-T6." Amado M., Carneiro F., Clausen H. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"A novel member of beta-1,3-galactosyltransferase family is down regulated during bladder TCC progression." Gromova I., Gromov P., Celis J.E. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Urinary bladder.
[5]	"Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family." Shiraishi N., Natsume A., Togayachi A., Endo T., Akashima T., Yamada Y., Imai N., Nakagawa S., Koizumi S., Sekine S., Narimatsu H., Sasaki K. J. Biol. Chem. 276:3498-3507(2001) [PubMed: 11042166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Testis.
[9]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 25-397. Tissue: Placenta.
[10]	"Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions." Amado M., Almeida R., Schwientek T., Clausen H. Biochim. Biophys. Acta 1473:35-53(1999) [PubMed: 10580128] [Abstract] Cited for: REVIEW.
[11]	"An unappreciated role for RNA surveillance." Hillman R.T., Green R.E., Brenner S.E. Genome Biol. 5:R8.1-R8.16(2004) [PubMed: 14759258] [Abstract] Cited for: SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
[12]	"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry." Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D. J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-79, MASS SPECTROMETRY. Tissue: Plasma.

Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF092051 mRNA. Translation: AAD09764.2. AJ006077 mRNA. Translation: CAB91546.1. AF288208 mRNA. Translation: AAF97253.1. AF288209 mRNA. Translation: AAF97254.1. AB049584 mRNA. Translation: BAB21530.1. BC030579 mRNA. Translation: AAH30579.1. AC093401 Genomic DNA. Translation: AAX93271.1. CH471053 Genomic DNA. Translation: EAW99977.1. CH471053 Genomic DNA. Translation: EAW99978.1. CH471053 Genomic DNA. Translation: EAW99979.1. BC047933 mRNA. Translation: AAH47933.1. AK002009 mRNA. Translation: BAA92031.1. Different initiation.
IPI	IPI00217345. IPI00257239.
RefSeq	NP_006568.2. NM_006577.5.
UniGene	Hs.173203.
3D structure databases
ProteinModelPortal	Q9NY97.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9NY97. 1 interaction.
STRING	Q9NY97.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
PTM databases
PhosphoSite	Q9NY97.
Polymorphism databases
DMDM	29840874.
Proteomic databases
PRIDE	Q9NY97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301998; ENSP00000305595; ENSG00000170340. ENST00000405767; ENSP00000384692; ENSG00000170340.
GeneID	10678.
KEGG	hsa:10678.
UCSC	uc002sbs.1. human.
Organism-specific databases
CTD	10678.
GeneCards	GC02P062423.
H-InvDB	HIX0002087.
HGNC	HGNC:15629. B3GNT2.
HPA	HPA005997.
MIM	605581. gene.
neXtProt	NX_Q9NY97.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00560000076821.
HOGENOM	HBG714704.
HOVERGEN	HBG050653.
InParanoid	Q9NY97.
OMA	MMVNVFI.
OrthoDB	EOG4RFKT0.
PhylomeDB	Q9NY97.
Gene expression databases
ArrayExpress	Q9NY97.
Bgee	Q9NY97.
CleanEx	HS_B3GNT1. HS_B3GNT2.
Genevestigator	Q9NY97.
GermOnline	ENSG00000170340. Homo sapiens.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
KO	K00741.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	40601.
SOURCE	Search...

Entry information

Entry name

B3GN2_HUMAN

Accession

Primary (citable) accession number: Q9NY97
Secondary accession number(s): Q54AC1 , Q9NQQ9, Q9NQR0, Q9NUT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	April 11, 2003
Last modified:	January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.