Q9NY64 (GTR8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 2, facilitated glucose transporter member 8

Alternative name(s):
Glucose transporter type 8
Short name=GLUT-8
Glucose transporter type X1

Gene names

Name:	SLC2A8
Synonyms:	GLUT8, GLUTX1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Insulin-regulated facilitative glucose transporter. Binds cytochalasin B in a glucose-inhibitable manner. Seems to be a dual-specific sugar transporter as it is inhibitable by fructose By similarity.
Subunit structure	Interacts with AP2B1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity. Note: Principally intracellular. May move between intracellular vesicles and the plasma membrane. The dileucine internalization motif is critical for intracellular sequestration By similarity.
Tissue specificity	Highly expressed in testis, but not in testicular carcinoma. Lower amounts present in most other tissues.
Induction	In testis, down-regulated by estrogen.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement Ref.2. Source: ProtInc insulin receptor signaling pathway Inferred from electronic annotation. Source: Compara male meiosis I Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc intracellular membrane-bounded organelle Inferred from direct assay. Source: HPA synaptic vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	glucose binding Inferred from electronic annotation. Source: Compara glucose transmembrane transporter activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Solute carrier family 2, facilitated glucose transporter member 8		PRO_0000050375
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 46	21	Helical; Name=1; Potential
	Topological domain	47 – 70	24	Extracellular Potential
	Transmembrane	71 – 91	21	Helical; Name=2; Potential
	Topological domain	92 – 96	5	Cytoplasmic Potential
	Transmembrane	97 – 117	21	Helical; Name=3; Potential
	Topological domain	118 – 127	10	Extracellular Potential
	Transmembrane	128 – 148	21	Helical; Name=4; Potential
	Topological domain	149 – 156	8	Cytoplasmic Potential
	Transmembrane	157 – 177	21	Helical; Name=5; Potential
	Topological domain	178 – 182	5	Extracellular Potential
	Transmembrane	183 – 203	21	Helical; Name=6; Potential
	Topological domain	204 – 256	53	Cytoplasmic Potential
	Transmembrane	257 – 277	21	Helical; Name=7; Potential
	Topological domain	278 – 292	15	Extracellular Potential
	Transmembrane	293 – 313	21	Helical; Name=8; Potential
	Topological domain	314 – 319	6	Cytoplasmic Potential
	Transmembrane	320 – 340	21	Helical; Name=9; Potential
	Topological domain	341 – 367	27	Extracellular Potential
	Transmembrane	368 – 388	21	Helical; Name=10; Potential
	Topological domain	389 – 404	16	Cytoplasmic Potential
	Transmembrane	405 – 425	21	Helical; Name=11; Potential
	Topological domain	426 – 438	13	Extracellular Potential
	Transmembrane	439 – 459	21	Helical; Name=12; Potential
	Topological domain	460 – 477	18	Cytoplasmic Potential
	Motif	12 – 13	2	Dileucine internalization motif Potential
Amino acid modifications
	Glycosylation	349	1	N-linked (GlcNAc...) By similarity
Natural variations
	Natural variant	253	1	I → T. Corresponds to variant rs34064803 [ dbSNP | Ensembl ].		VAR_061880
Experimental info
	Sequence conflict	377	1	S → N in CAB75702. Ref.2
	Sequence conflict	456 – 457	2	LF → FS in CAB89809. Ref.1
	Sequence conflict	462	1	T → I in CAB75702. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9NY64 [UniParc]. Last modified April 17, 2007. Version 3. Checksum: 0B480F94AF063316	FASTA	477	50,819
	10 20 30 40 50 60 MTPEDPEETQ PLLGPPGGSA PRGRRVFLAA FAAALGPLSF GFALGYSSPA IPSLQRAAPP 70 80 90 100 110 120 APRLDDAAAS WFGAVVTLGA AAGGVLGGWL VDRAGRKLSL LLCSVPFVAG FAVITAAQDV 130 140 150 160 170 180 WMLLGGRLLT GLACGVASLV APVYISEIAY PAVRGLLGSC VQLMVVVGIL LAYLAGWVLE 190 200 210 220 230 240 WRWLAVLGCV PPSLMLLLMC FMPETPRFLL TQHRRQEAMA ALRFLWGSEQ GWEDPPIGAE 250 260 270 280 290 300 QSFHLALLRQ PGIYKPFIIG VSLMAFQQLS GVNAVMFYAE TIFEEAKFKD SSLASVVVGV 310 320 330 340 350 360 IQVLFTAVAA LIMDRAGRRL LLVLSGVVMV FSTSAFGAYF KLTQGGPGNS SHVAISAPVS 370 380 390 400 410 420 AQPVDASVGL AWLAVGSMCL FIAGFAVGWG PIPWLLMSEI FPLHVKGVAT GICVLTNWLM 430 440 450 460 470 AFLVTKEFSS LMEVLRPYGA FWLASAFCIF SVLFTLFCVP ETKGKTLEQI TAHFEGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GLUT8, a novel member of the sugar transport facilitator family with glucose transport activity." Doege H., Schuermann A., Bahrenberg G., Brauers A., Joost H.-G. J. Biol. Chem. 275:16275-16280(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"GLUTX1, a novel mammalian glucose transporter expressed in the central nervous system and insulin-sensitive tissues." Ibberson M.R., Uldry M.A., Thorens B. J. Biol. Chem. 275:4607-4612(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y17801 mRNA. Translation: CAB89809.1. AJ245937 mRNA. Translation: CAB75702.1. AL445222 Genomic DNA. Translation: CAH72918.1. CH471090 Genomic DNA. Translation: EAW87660.1. BC019043 mRNA. Translation: AAH19043.1.
IPI	IPI00302242.
RefSeq	NP_001258640.1. NM_001271711.1. NP_001258641.1. NM_001271712.1. NP_055395.2. NM_014580.4.
UniGene	Hs.179522.
3D structure databases
ProteinModelPortal	Q9NY64.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000362469.
PTM databases
PhosphoSite	Q9NY64.
Polymorphism databases
DMDM	145559479.
Proteomic databases
PaxDb	Q9NY64.
PRIDE	Q9NY64.
Protocols and materials databases
DNASU	29988.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000373371; ENSP00000362469; ENSG00000136856.
GeneID	29988.
KEGG	hsa:29988.
UCSC	uc004bqu.3. human.
Organism-specific databases
CTD	29988.
GeneCards	GC09P130159.
HGNC	HGNC:13812. SLC2A8.
HPA	HPA011935.
MIM	605245. gene.
neXtProt	NX_Q9NY64.
PharmGKB	PA37813.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOGENOM	HOG000202868.
HOVERGEN	HBG104335.
InParanoid	Q9NY64.
KO	K08145.
OMA	WEDPPIG.
OrthoDB	EOG41G349.
PhylomeDB	Q9NY64.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q9NY64.
Bgee	Q9NY64.
CleanEx	HS_SLC2A8.
Genevestigator	Q9NY64.
GermOnline	ENSG00000136856. Homo sapiens.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR003663. Sugar/inositol_transpt. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
PRINTS	PR00171. SUGRTRNSPORT.
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00879. SP. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 2 hits. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	29988.
NextBio	52760.
SOURCE	Search...

Entry information

Entry name

GTR8_HUMAN

Accession

Primary (citable) accession number: Q9NY64
Secondary accession number(s): Q8WUZ9, Q9NSC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	April 17, 2007
Last modified:	April 3, 2013
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.