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Protein

Dipeptidyl peptidase 3

Gene

DPP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves and degrades of the opioid peptide enkephalin. Also cleaves Arg-Arg-beta-naphthylamide.1 Publication

Catalytic activityi

Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Enzyme regulationi

Inhibited by spinorphin, an opioid peptide derived from hemoglobin.1 Publication

pH dependencei

Optimum pH is 8.8.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi450Zinc; catalytic1 Publication1
Active sitei451By similarity1
Metal bindingi455Zinc; catalytic1 Publication1
Metal bindingi508Zinc; catalytic1 Publication1

GO - Molecular functioni

  • dipeptidyl-peptidase activity Source: UniProtKB
  • metallopeptidase activity Source: UniProtKB-KW
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS10803-MONOMER.
BRENDAi3.4.14.4. 2681.

Protein family/group databases

MEROPSiM49.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidyl peptidase 3 (EC:3.4.14.4)
Alternative name(s):
Dipeptidyl aminopeptidase III
Dipeptidyl arylamidase III
Dipeptidyl peptidase III
Short name:
DPP III
Enkephalinase B
Gene namesi
Name:DPP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:3008. DPP3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: HPA
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000254986.
PharmGKBiPA27466.

Chemistry databases

ChEMBLiCHEMBL4520.

Polymorphism and mutation databases

BioMutaiDPP3.
DMDMi20532389.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000782382 – 737Dipeptidyl peptidase 3Add BLAST736

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9NY33.
MaxQBiQ9NY33.
PaxDbiQ9NY33.
PeptideAtlasiQ9NY33.
PRIDEiQ9NY33.

PTM databases

iPTMnetiQ9NY33.
PhosphoSitePlusiQ9NY33.
SwissPalmiQ9NY33.

Expressioni

Gene expression databases

BgeeiENSG00000254986.
CleanExiHS_DPP3.
ExpressionAtlasiQ9NY33. baseline and differential.
GenevisibleiQ9NY33. HS.

Organism-specific databases

HPAiHPA035780.
HPA035781.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KEAP1Q141455EBI-718333,EBI-751001

Protein-protein interaction databases

BioGridi115383. 23 interactors.
DIPiDIP-53793N.
IntActiQ9NY33. 8 interactors.
STRINGi9606.ENSP00000353701.

Chemistry databases

BindingDBiQ9NY33.

Structurei

Secondary structure

1737
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi14 – 16Combined sources3
Helixi20 – 25Combined sources6
Helixi28 – 50Combined sources23
Helixi56 – 69Combined sources14
Helixi72 – 81Combined sources10
Helixi86 – 102Combined sources17
Beta strandi104 – 106Combined sources3
Turni108 – 110Combined sources3
Helixi120 – 128Combined sources9
Helixi131 – 135Combined sources5
Helixi137 – 152Combined sources16
Helixi156 – 158Combined sources3
Beta strandi159 – 161Combined sources3
Helixi164 – 166Combined sources3
Beta strandi170 – 172Combined sources3
Helixi178 – 190Combined sources13
Beta strandi198 – 204Combined sources7
Beta strandi206 – 208Combined sources3
Beta strandi210 – 217Combined sources8
Beta strandi222 – 224Combined sources3
Helixi231 – 233Combined sources3
Beta strandi235 – 239Combined sources5
Beta strandi242 – 250Combined sources9
Helixi252 – 266Combined sources15
Helixi272 – 287Combined sources16
Helixi290 – 302Combined sources13
Beta strandi307 – 316Combined sources10
Beta strandi318 – 323Combined sources6
Beta strandi327 – 335Combined sources9
Helixi337 – 346Combined sources10
Helixi350 – 353Combined sources4
Helixi354 – 356Combined sources3
Beta strandi357 – 359Combined sources3
Helixi361 – 363Combined sources3
Beta strandi372 – 384Combined sources13
Beta strandi388 – 392Combined sources5
Helixi396 – 401Combined sources6
Beta strandi405 – 409Combined sources5
Helixi410 – 416Combined sources7
Helixi421 – 423Combined sources3
Helixi429 – 452Combined sources24
Turni453 – 456Combined sources4
Beta strandi465 – 467Combined sources3
Beta strandi469 – 471Combined sources3
Turni473 – 475Combined sources3
Turni479 – 481Combined sources3
Beta strandi482 – 484Combined sources3
Helixi495 – 499Combined sources5
Helixi500 – 502Combined sources3
Helixi503 – 518Combined sources16
Helixi524 – 527Combined sources4
Helixi533 – 552Combined sources20
Helixi553 – 556Combined sources4
Turni559 – 562Combined sources4
Beta strandi563 – 565Combined sources3
Helixi567 – 581Combined sources15
Beta strandi586 – 593Combined sources8
Beta strandi597 – 605Combined sources9
Helixi607 – 609Combined sources3
Turni610 – 613Combined sources4
Helixi614 – 630Combined sources17
Helixi634 – 644Combined sources11
Turni651 – 653Combined sources3
Helixi655 – 664Combined sources10
Beta strandi671 – 673Combined sources3
Beta strandi676 – 680Combined sources5
Beta strandi683 – 687Combined sources5
Helixi693 – 701Combined sources9
Turni705 – 707Combined sources3
Helixi708 – 721Combined sources14
Helixi722 – 725Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FVYX-ray1.90A1-726[»]
3T6BX-ray2.40A/B1-726[»]
3T6JX-ray2.98A1-726[»]
5E2QX-ray2.40A1-726[»]
5E33X-ray1.84A1-726[»]
5E3AX-ray2.05A1-726[»]
5E3CX-ray2.77A1-726[»]
5EGYX-ray2.74A1-726[»]
5EHHX-ray2.38A1-726[»]
ProteinModelPortaliQ9NY33.
SMRiQ9NY33.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NY33.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M49 family.Curated

Phylogenomic databases

eggNOGiKOG3675. Eukaryota.
ENOG410XRK4. LUCA.
GeneTreeiENSGT00390000007335.
HOGENOMiHOG000187784.
HOVERGENiHBG025680.
InParanoidiQ9NY33.
KOiK01277.
PhylomeDBiQ9NY33.
TreeFamiTF300598.

Family and domain databases

InterProiIPR005317. Dipeptidyl-peptase3.
[Graphical view]
PIRSFiPIRSF007828. Dipeptidyl-peptidase_III. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NY33-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADTQYILPN DIGVSSLDCR EAFRLLSPTE RLYAYHLSRA AWYGGLAVLL
60 70 80 90 100
QTSPEAPYIY ALLSRLFRAQ DPDQLRQHAL AEGLTEEEYQ AFLVYAAGVY
110 120 130 140 150
SNMGNYKSFG DTKFVPNLPK EKLERVILGS EAAQQHPEEV RGLWQTCGEL
160 170 180 190 200
MFSLEPRLRH LGLGKEGITT YFSGNCTMED AKLAQDFLDS QNLSAYNTRL
210 220 230 240 250
FKEVDGEGKP YYEVRLASVL GSEPSLDSEV TSKLKSYEFR GSPFQVTRGD
260 270 280 290 300
YAPILQKVVE QLEKAKAYAA NSHQGQMLAQ YIESFTQGSI EAHKRGSRFW
310 320 330 340 350
IQDKGPIVES YIGFIESYRD PFGSRGEFEG FVAVVNKAMS AKFERLVASA
360 370 380 390 400
EQLLKELPWP PTFEKDKFLT PDFTSLDVLT FAGSGIPAGI NIPNYDDLRQ
410 420 430 440 450
TEGFKNVSLG NVLAVAYATQ REKLTFLEED DKDLYILWKG PSFDVQVGLH
460 470 480 490 500
ELLGHGSGKL FVQDEKGAFN FDQETVINPE TGEQIQSWYR SGETWDSKFS
510 520 530 540 550
TIASSYEECR AESVGLYLCL HPQVLEIFGF EGADAEDVIY VNWLNMVRAG
560 570 580 590 600
LLALEFYTPE AFNWRQAHMQ ARFVILRVLL EAGEGLVTIT PTTGSDGRPD
610 620 630 640 650
ARVRLDRSKI RSVGKPALER FLRRLQVLKS TGDVAGGRAL YEGYATVTDA
660 670 680 690 700
PPECFLTLRD TVLLRKESRK LIVQPNTRLE GSDVQLLEYE ASAAGLIRSF
710 720 730
SERFPEDGPE LEEILTQLAT ADARFWKGPS EAPSGQA
Length:737
Mass (Da):82,589
Last modified:May 10, 2002 - v2
Checksum:iE2BB1923C9CAFDEC
GO
Isoform 2 (identifier: Q9NY33-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     182-601: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:317
Mass (Da):35,489
Checksum:iA172153E757C72E5
GO
Isoform 3 (identifier: Q8NFJ9-2) [UniParc]FASTAAdd to basket
Also known as: DPP3-BBS1
The sequence of this isoform can be found in the external entry Q8NFJ9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a readthrough transcript which may produce a DPP3-BBS1 fusion protein. No experimental confirmation available.
Length:630
Mass (Da):69,658
GO
Isoform 4 (identifier: Q9NY33-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     91-120: Missing.

Note: No experimental confirmation available.
Show »
Length:707
Mass (Da):79,307
Checksum:i52EAAAFC59AB9AFA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224P → S in BAG59686 (PubMed:14702039).Curated1
Sequence conflicti417 – 419YAT → TA in BAA75785 (PubMed:9425109).Curated3
Sequence conflicti697I → Y in BAA75785 (PubMed:9425109).Curated1

Mass spectrometryi

Isoform 1 : Molecular mass is 82500±60 Da from positions 1 - 737. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03349476R → H.1 PublicationCorresponds to variant rs11826683dbSNPEnsembl.1
Natural variantiVAR_033495145Q → H.1 PublicationCorresponds to variant rs11550299dbSNPEnsembl.1
Natural variantiVAR_021850678R → H.2 PublicationsCorresponds to variant rs2305535dbSNPEnsembl.1
Natural variantiVAR_051597690E → K.Corresponds to variant rs12421620dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04469691 – 120Missing in isoform 4. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_005510182 – 601Missing in isoform 2. 1 PublicationAdd BLAST420

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271216 mRNA. Translation: CAB72433.1.
AK021449 mRNA. Translation: BAB13828.1.
AK297199 mRNA. Translation: BAG59686.1.
AK315478 mRNA. Translation: BAG37862.1.
AP002748 Genomic DNA. No translation available.
BC001446 mRNA. Translation: AAH01446.1.
BC007221 mRNA. Translation: AAH07221.1.
BC014038 mRNA. Translation: AAH14038.1.
BC024271 mRNA. Translation: AAH24271.1.
AB017970 mRNA. Translation: BAA75785.1.
CCDSiCCDS58147.1. [Q9NY33-4]
CCDS8141.1. [Q9NY33-1]
RefSeqiNP_001243599.1. NM_001256670.1. [Q9NY33-4]
NP_005691.2. NM_005700.4. [Q9NY33-1]
NP_569710.2. NM_130443.3. [Q9NY33-1]
UniGeneiHs.502914.
Hs.740670.

Genome annotation databases

EnsembliENST00000530165; ENSP00000436941; ENSG00000254986. [Q9NY33-4]
ENST00000541961; ENSP00000440502; ENSG00000254986. [Q9NY33-1]
GeneIDi10072.
KEGGihsa:10072.
UCSCiuc010rpe.3. human. [Q9NY33-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271216 mRNA. Translation: CAB72433.1.
AK021449 mRNA. Translation: BAB13828.1.
AK297199 mRNA. Translation: BAG59686.1.
AK315478 mRNA. Translation: BAG37862.1.
AP002748 Genomic DNA. No translation available.
BC001446 mRNA. Translation: AAH01446.1.
BC007221 mRNA. Translation: AAH07221.1.
BC014038 mRNA. Translation: AAH14038.1.
BC024271 mRNA. Translation: AAH24271.1.
AB017970 mRNA. Translation: BAA75785.1.
CCDSiCCDS58147.1. [Q9NY33-4]
CCDS8141.1. [Q9NY33-1]
RefSeqiNP_001243599.1. NM_001256670.1. [Q9NY33-4]
NP_005691.2. NM_005700.4. [Q9NY33-1]
NP_569710.2. NM_130443.3. [Q9NY33-1]
UniGeneiHs.502914.
Hs.740670.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FVYX-ray1.90A1-726[»]
3T6BX-ray2.40A/B1-726[»]
3T6JX-ray2.98A1-726[»]
5E2QX-ray2.40A1-726[»]
5E33X-ray1.84A1-726[»]
5E3AX-ray2.05A1-726[»]
5E3CX-ray2.77A1-726[»]
5EGYX-ray2.74A1-726[»]
5EHHX-ray2.38A1-726[»]
ProteinModelPortaliQ9NY33.
SMRiQ9NY33.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115383. 23 interactors.
DIPiDIP-53793N.
IntActiQ9NY33. 8 interactors.
STRINGi9606.ENSP00000353701.

Chemistry databases

BindingDBiQ9NY33.
ChEMBLiCHEMBL4520.

Protein family/group databases

MEROPSiM49.001.

PTM databases

iPTMnetiQ9NY33.
PhosphoSitePlusiQ9NY33.
SwissPalmiQ9NY33.

Polymorphism and mutation databases

BioMutaiDPP3.
DMDMi20532389.

Proteomic databases

EPDiQ9NY33.
MaxQBiQ9NY33.
PaxDbiQ9NY33.
PeptideAtlasiQ9NY33.
PRIDEiQ9NY33.

Protocols and materials databases

DNASUi10072.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000530165; ENSP00000436941; ENSG00000254986. [Q9NY33-4]
ENST00000541961; ENSP00000440502; ENSG00000254986. [Q9NY33-1]
GeneIDi10072.
KEGGihsa:10072.
UCSCiuc010rpe.3. human. [Q9NY33-1]

Organism-specific databases

CTDi10072.
GeneCardsiDPP3.
HGNCiHGNC:3008. DPP3.
HPAiHPA035780.
HPA035781.
MIMi606818. gene.
neXtProtiNX_Q9NY33.
OpenTargetsiENSG00000254986.
PharmGKBiPA27466.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3675. Eukaryota.
ENOG410XRK4. LUCA.
GeneTreeiENSGT00390000007335.
HOGENOMiHOG000187784.
HOVERGENiHBG025680.
InParanoidiQ9NY33.
KOiK01277.
PhylomeDBiQ9NY33.
TreeFamiTF300598.

Enzyme and pathway databases

BioCyciZFISH:HS10803-MONOMER.
BRENDAi3.4.14.4. 2681.

Miscellaneous databases

ChiTaRSiDPP3. human.
EvolutionaryTraceiQ9NY33.
GeneWikiiDPP3.
GenomeRNAii10072.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000254986.
CleanExiHS_DPP3.
ExpressionAtlasiQ9NY33. baseline and differential.
GenevisibleiQ9NY33. HS.

Family and domain databases

InterProiIPR005317. Dipeptidyl-peptase3.
[Graphical view]
PIRSFiPIRSF007828. Dipeptidyl-peptidase_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPP3_HUMAN
AccessioniPrimary (citable) accession number: Q9NY33
Secondary accession number(s): B2RDB5
, B4DLX4, F5H8L6, O95748, Q969H2, Q9BV67, Q9HAL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 10, 2002
Last modified: November 2, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.