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Protein

Probable ATP-dependent RNA helicase DDX43

Gene

DDX43

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi286 – 2938ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: ProtInc
  • RNA binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX43 (EC:3.6.4.13)
Alternative name(s):
Cancer/testis antigen 13
Short name:
CT13
DEAD box protein 43
DEAD box protein HAGE
Helical antigen
Gene namesi
Name:DDX43
Synonyms:HAGE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:18677. DDX43.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134988734.

Polymorphism and mutation databases

BioMutaiDDX43.
DMDMi145559466.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Probable ATP-dependent RNA helicase DDX43PRO_0000054972Add
BLAST

Proteomic databases

EPDiQ9NXZ2.
MaxQBiQ9NXZ2.
PaxDbiQ9NXZ2.
PeptideAtlasiQ9NXZ2.
PRIDEiQ9NXZ2.

PTM databases

iPTMnetiQ9NXZ2.
PhosphoSiteiQ9NXZ2.

Expressioni

Tissue specificityi

Expressed in testis. Expressed in many tumors of various histological types at a level that is 100-fold higher than the level observed in normal tissues except testis.1 Publication

Gene expression databases

BgeeiENSG00000080007.
CleanExiHS_DDX43.
GenevisibleiQ9NXZ2. HS.

Organism-specific databases

HPAiHPA031380.
HPA031381.

Interactioni

Protein-protein interaction databases

BioGridi120690. 10 interactions.
MINTiMINT-3076443.
STRINGi9606.ENSP00000359361.

Structurei

3D structure databases

ProteinModelPortaliQ9NXZ2.
SMRiQ9NXZ2. Positions 190-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 12862KHPROSITE-ProRule annotationAdd
BLAST
Domaini273 – 448176Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini460 – 621162Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi242 – 27029Q motifAdd
BLAST
Motifi396 – 3994DEAD box

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 KH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0336. Eukaryota.
ENOG410XR3V. LUCA.
GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268804.
HOVERGENiHBG015893.
InParanoidiQ9NXZ2.
KOiK17043.
OMAiHTEYQST.
OrthoDBiEOG091G05VF.
PhylomeDBiQ9NXZ2.
TreeFamiTF312949.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00013. KH_1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54791. SSF54791. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50084. KH_TYPE_1. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NXZ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSHHGGAPKA STWVVASRRS STVSRAPERR PAEELNRTGP EGYSVGRGGR
60 70 80 90 100
WRGTSRPPEA VAAGHEELPL CFALKSHFVG AVIGRGGSKI KNIQSTTNTT
110 120 130 140 150
IQIIQEQPES LVKIFGSKAM QTKAKAVIDN FVKKLEENYN SECGIDTAFQ
160 170 180 190 200
PSVGKDGSTD NNVVAGDRPL IDWDQIREEG LKWQKTKWAD LPPIKKNFYK
210 220 230 240 250
ESTATSAMSK VEADSWRKEN FNITWDDLKD GEKRPIPNPT CTFDDAFQCY
260 270 280 290 300
PEVMENIKKA GFQKPTPIQS QAWPIVLQGI DLIGVAQTGT GKTLCYLMPG
310 320 330 340 350
FIHLVLQPSL KGQRNRPGML VLTPTRELAL QVEGECCKYS YKGLRSVCVY
360 370 380 390 400
GGGNRDEQIE ELKKGVDIII ATPGRLNDLQ MSNFVNLKNI TYLVLDEADK
410 420 430 440 450
MLDMGFEPQI MKILLDVRPD RQTVMTSATW PHSVHRLAQS YLKEPMIVYV
460 470 480 490 500
GTLDLVAVSS VKQNIIVTTE EEKWSHMQTF LQSMSSTDKV IVFVSRKAVA
510 520 530 540 550
DHLSSDLILG NISVESLHGD REQRDREKAL ENFKTGKVRI LIATDLASRG
560 570 580 590 600
LDVHDVTHVY NFDFPRNIEE YVHRIGRTGR AGRTGVSITT LTRNDWRVAS
610 620 630 640
ELINILERAN QSIPEELVSM AERFKAHQQK REMERKMERP QGRPKKFH
Length:648
Mass (Da):72,844
Last modified:April 17, 2007 - v2
Checksum:i4E09F15D36EFC3E3
GO
Isoform 2 (identifier: Q9NXZ2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     190-195: DLPPIK → GKKILI
     196-648: Missing.

Note: No experimental confirmation available.
Show »
Length:195
Mass (Da):21,295
Checksum:i88A7BC21560E27D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601A → D in AAH66938 (PubMed:15489334).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti625 – 6251K → E.2 Publications
Corresponds to variant rs311686 [ dbSNP | Ensembl ].
VAR_057234
Natural varianti629 – 6291Q → R.2 Publications
Corresponds to variant rs311685 [ dbSNP | Ensembl ].
VAR_057235

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei190 – 1956DLPPIK → GKKILI in isoform 2. 1 PublicationVSP_056955
Alternative sequencei196 – 648453Missing in isoform 2. 1 PublicationVSP_056956Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278110 mRNA. Translation: CAB92442.1.
AL136751 mRNA. Translation: CAB66685.1.
AK303324 mRNA. Translation: BAG64390.1.
AC019205 Genomic DNA. No translation available.
BC066938 mRNA. Translation: AAH66938.1.
CCDSiCCDS4977.1. [Q9NXZ2-1]
RefSeqiNP_061135.2. NM_018665.2. [Q9NXZ2-1]
UniGeneiHs.125507.

Genome annotation databases

EnsembliENST00000370336; ENSP00000359361; ENSG00000080007. [Q9NXZ2-1]
GeneIDi55510.
KEGGihsa:55510.
UCSCiuc003pgw.4. human. [Q9NXZ2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278110 mRNA. Translation: CAB92442.1.
AL136751 mRNA. Translation: CAB66685.1.
AK303324 mRNA. Translation: BAG64390.1.
AC019205 Genomic DNA. No translation available.
BC066938 mRNA. Translation: AAH66938.1.
CCDSiCCDS4977.1. [Q9NXZ2-1]
RefSeqiNP_061135.2. NM_018665.2. [Q9NXZ2-1]
UniGeneiHs.125507.

3D structure databases

ProteinModelPortaliQ9NXZ2.
SMRiQ9NXZ2. Positions 190-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120690. 10 interactions.
MINTiMINT-3076443.
STRINGi9606.ENSP00000359361.

PTM databases

iPTMnetiQ9NXZ2.
PhosphoSiteiQ9NXZ2.

Polymorphism and mutation databases

BioMutaiDDX43.
DMDMi145559466.

Proteomic databases

EPDiQ9NXZ2.
MaxQBiQ9NXZ2.
PaxDbiQ9NXZ2.
PeptideAtlasiQ9NXZ2.
PRIDEiQ9NXZ2.

Protocols and materials databases

DNASUi55510.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370336; ENSP00000359361; ENSG00000080007. [Q9NXZ2-1]
GeneIDi55510.
KEGGihsa:55510.
UCSCiuc003pgw.4. human. [Q9NXZ2-1]

Organism-specific databases

CTDi55510.
GeneCardsiDDX43.
HGNCiHGNC:18677. DDX43.
HPAiHPA031380.
HPA031381.
MIMi606286. gene.
neXtProtiNX_Q9NXZ2.
PharmGKBiPA134988734.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0336. Eukaryota.
ENOG410XR3V. LUCA.
GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268804.
HOVERGENiHBG015893.
InParanoidiQ9NXZ2.
KOiK17043.
OMAiHTEYQST.
OrthoDBiEOG091G05VF.
PhylomeDBiQ9NXZ2.
TreeFamiTF312949.

Miscellaneous databases

ChiTaRSiDDX43. human.
GeneWikiiDDX43.
GenomeRNAii55510.
PROiQ9NXZ2.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000080007.
CleanExiHS_DDX43.
GenevisibleiQ9NXZ2. HS.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00013. KH_1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54791. SSF54791. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50084. KH_TYPE_1. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX43_HUMAN
AccessioniPrimary (citable) accession number: Q9NXZ2
Secondary accession number(s): B4E0C8, Q6NXR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.