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Protein

DnaJ homolog subfamily B member 12

Gene

DNAJB12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 12
Gene namesi
Name:DNAJB12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:14891. DNAJB12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei244 – 26421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27414.

Polymorphism and mutation databases

BioMutaiDNAJB12.
DMDMi294862531.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375DnaJ homolog subfamily B member 12PRO_0000071037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9NXW2.
MaxQBiQ9NXW2.
PaxDbiQ9NXW2.
PeptideAtlasiQ9NXW2.
PRIDEiQ9NXW2.

PTM databases

iPTMnetiQ9NXW2.
PhosphoSiteiQ9NXW2.

Expressioni

Gene expression databases

BgeeiQ9NXW2.
CleanExiHS_DNAJB12.
ExpressionAtlasiQ9NXW2. baseline and differential.
GenevisibleiQ9NXW2. HS.

Organism-specific databases

HPAiHPA010642.

Interactioni

Protein-protein interaction databases

BioGridi120150. 10 interactions.
IntActiQ9NXW2. 6 interactions.
MINTiMINT-1145606.
STRINGi9606.ENSP00000345575.

Structurei

Secondary structure

1
375
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi111 – 1155Combined sources
Helixi123 – 13412Combined sources
Turni139 – 1413Combined sources
Helixi145 – 16117Combined sources
Helixi164 – 1729Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CTPNMR-A110-174[»]
ProteinModelPortaliQ9NXW2.
SMRiQ9NXW2. Positions 109-212.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NXW2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini112 – 17665JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ9NXW2.
KOiK09518.
OrthoDBiEOG7G4QF8.
PhylomeDBiQ9NXW2.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9NXW2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESNKDEAER CISIALKAIQ SNQPDRALRF LEKAQRLYPT PRVRALIESL
60 70 80 90 100
NQKPQTAGDQ PPPTDTTHAT HRKAGGTDAP SANGEAGGES TKGYTAEQVA
110 120 130 140 150
AVKRVKQCKD YYEILGVSRG ASDEDLKKAY RRLALKFHPD KNHAPGATEA
160 170 180 190 200
FKAIGTAYAV LSNPEKRKQY DQFGDDKSQA ARHGHGHGDF HRGFEADISP
210 220 230 240 250
EDLFNMFFGG GFPSSNVHVY SNGRMRYTYQ QRQDRRDNQG DGGLGVFVQL
260 270 280 290 300
MPILILILVS ALSQLMVSSP PYSLSPRPSV GHIHRRVTDH LGVVYYVGDT
310 320 330 340 350
FSEEYTGSSL KTVERNVEDD YIANLRNNCW KEKQQSEGLL YRARYFGDTD
360 370
MYHRAQKMGT PSCSRLSEVQ ASLHG
Length:375
Mass (Da):41,819
Last modified:April 20, 2010 - v4
Checksum:i27893FF3E22EF778
GO
Isoform 2 (identifier: Q9NXW2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     369-375: VQASLHG → TMKSLENFW

Show »
Length:377
Mass (Da):42,263
Checksum:i80AF0BB3CFF3ECDE
GO

Sequence cautioni

The sequence BAB15289.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAH13619.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701T → S in BAA90896 (PubMed:14702039).Curated
Sequence conflicti212 – 2121F → S in BAA90896 (PubMed:14702039).Curated
Sequence conflicti215 – 2151S → G in BAH13619 (PubMed:14702039).Curated
Sequence conflicti336 – 3361S → K in BAA90896 (PubMed:14702039).Curated
Sequence conflicti336 – 3361S → K in BAB15289 (PubMed:14702039).Curated
Sequence conflicti336 – 3361S → K in BAH13619 (PubMed:14702039).Curated
Sequence conflicti336 – 3361S → K in AAH11812 (PubMed:15164054).Curated
Sequence conflicti336 – 3361S → K in AAH64920 (PubMed:15164054).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti304 – 3041E → K.
Corresponds to variant rs3750784 [ dbSNP | Ensembl ].
VAR_017864

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei369 – 3757VQASLHG → TMKSLENFW in isoform 2. 1 PublicationVSP_039040

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000034 mRNA. Translation: BAA90896.1.
AK025942 mRNA. Translation: BAB15289.1. Different initiation.
AK302071 mRNA. Translation: BAH13619.1. Different initiation.
AC091769 Genomic DNA. No translation available.
BC011812 mRNA. Translation: AAH11812.2.
BC064920 mRNA. Translation: AAH64920.1.
RefSeqiNP_001002762.2. NM_001002762.2.
NP_060096.3. NM_017626.4.
UniGeneiHs.500013.

Genome annotation databases

EnsembliENST00000444643; ENSP00000403313; ENSG00000148719.
GeneIDi54788.
KEGGihsa:54788.
UCSCiuc057tyc.1. human. [Q9NXW2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000034 mRNA. Translation: BAA90896.1.
AK025942 mRNA. Translation: BAB15289.1. Different initiation.
AK302071 mRNA. Translation: BAH13619.1. Different initiation.
AC091769 Genomic DNA. No translation available.
BC011812 mRNA. Translation: AAH11812.2.
BC064920 mRNA. Translation: AAH64920.1.
RefSeqiNP_001002762.2. NM_001002762.2.
NP_060096.3. NM_017626.4.
UniGeneiHs.500013.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CTPNMR-A110-174[»]
ProteinModelPortaliQ9NXW2.
SMRiQ9NXW2. Positions 109-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120150. 10 interactions.
IntActiQ9NXW2. 6 interactions.
MINTiMINT-1145606.
STRINGi9606.ENSP00000345575.

PTM databases

iPTMnetiQ9NXW2.
PhosphoSiteiQ9NXW2.

Polymorphism and mutation databases

BioMutaiDNAJB12.
DMDMi294862531.

Proteomic databases

EPDiQ9NXW2.
MaxQBiQ9NXW2.
PaxDbiQ9NXW2.
PeptideAtlasiQ9NXW2.
PRIDEiQ9NXW2.

Protocols and materials databases

DNASUi54788.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000444643; ENSP00000403313; ENSG00000148719.
GeneIDi54788.
KEGGihsa:54788.
UCSCiuc057tyc.1. human. [Q9NXW2-1]

Organism-specific databases

CTDi54788.
GeneCardsiDNAJB12.
H-InvDBHIX0127298.
HGNCiHGNC:14891. DNAJB12.
HPAiHPA010642.
MIMi608376. gene.
neXtProtiNX_Q9NXW2.
PharmGKBiPA27414.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
HOGENOMiHOG000233608.
HOVERGENiHBG051373.
InParanoidiQ9NXW2.
KOiK09518.
OrthoDBiEOG7G4QF8.
PhylomeDBiQ9NXW2.

Miscellaneous databases

ChiTaRSiDNAJB12. human.
EvolutionaryTraceiQ9NXW2.
GenomeRNAii54788.
PROiQ9NXW2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9NXW2.
CleanExiHS_DNAJB12.
ExpressionAtlasiQ9NXW2. baseline and differential.
GenevisibleiQ9NXW2. HS.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR015399. DUF1977_DnaJ-like.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF09320. DUF1977. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Adipose tissue, Kidney epithelium and Testis.
  2. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Colon and Pancreas.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Solution structure of J-domain from human DnaJ subfamily B member 12."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 110-174.

Entry informationi

Entry nameiDJB12_HUMAN
AccessioniPrimary (citable) accession number: Q9NXW2
Secondary accession number(s): B7Z7I3, Q9H6H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: April 20, 2010
Last modified: July 6, 2016
This is version 141 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.