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Q9NXS2

- QPCTL_HUMAN

UniProt

Q9NXS2 - QPCTL_HUMAN

Protein

Glutaminyl-peptide cyclotransferase-like protein

Gene

QPCTL

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (18 May 2010)
      Previous versions | rss
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    Functioni

    Responsible for the biosynthesis of pyroglutamyl peptides.2 Publications

    Catalytic activityi

    L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3.2 Publications

    Cofactori

    Binds 1 zinc ion per subunit.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi186 – 1861Zinc; catalytic
    Active sitei225 – 2251Proton acceptor
    Metal bindingi226 – 2261Zinc; catalytic
    Active sitei269 – 2691Proton acceptor
    Metal bindingi351 – 3511Zinc; catalytic

    GO - Molecular functioni

    1. glutaminyl-peptide cyclotransferase activity Source: UniProtKB
    2. peptidase activity Source: InterPro
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM28.016.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminyl-peptide cyclotransferase-like protein (EC:2.3.2.5)
    Alternative name(s):
    Golgi-resident glutaminyl-peptide cyclotransferase
    isoQC
    Short name:
    gQC
    Gene namesi
    Name:QPCTL
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:25952. QPCTL.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Single-pass type I membrane protein 1 Publication

    GO - Cellular componenti

    1. Golgi apparatus Source: MGI
    2. Golgi membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134922163.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382Glutaminyl-peptide cyclotransferase-like proteinPRO_0000302002Add
    BLAST

    Proteomic databases

    MaxQBiQ9NXS2.
    PaxDbiQ9NXS2.
    PRIDEiQ9NXS2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9NXS2.
    BgeeiQ9NXS2.
    CleanExiHS_QPCTL.
    GenevestigatoriQ9NXS2.

    Organism-specific databases

    HPAiHPA040797.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    POLR1EQ9GZS11EBI-1052839,EBI-359458

    Protein-protein interaction databases

    BioGridi120171. 14 interactions.
    IntActiQ9NXS2. 5 interactions.
    STRINGi9606.ENSP00000012049.

    Structurei

    Secondary structure

    1
    382
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi78 – 869
    Helixi90 – 967
    Helixi99 – 1013
    Helixi110 – 12516
    Beta strandi131 – 14111
    Beta strandi144 – 15512
    Beta strandi159 – 16810
    Beta strandi176 – 1794
    Turni184 – 1874
    Helixi188 – 20013
    Helixi202 – 2109
    Beta strandi214 – 22310
    Beta strandi228 – 2303
    Helixi238 – 24912
    Beta strandi253 – 2575
    Turni258 – 2614
    Beta strandi262 – 2709
    Beta strandi272 – 2754
    Helixi283 – 2853
    Helixi286 – 30116
    Beta strandi313 – 3153
    Helixi329 – 3324
    Turni333 – 3353
    Beta strandi338 – 3425
    Turni348 – 3514
    Helixi357 – 3593
    Helixi362 – 38019

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3PB4X-ray1.13X53-382[»]
    3PB6X-ray1.05X53-382[»]
    3PB7X-ray1.40X53-382[»]
    3PB8X-ray1.13X53-382[»]
    3PB9X-ray1.12X53-382[»]
    ProteinModelPortaliQ9NXS2.
    SMRiQ9NXS2. Positions 70-382.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9NXS2.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei35 – 5521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG298226.
    HOGENOMiHOG000189291.
    HOVERGENiHBG009812.
    InParanoidiQ9NXS2.
    OMAiQTYFRKD.
    OrthoDBiEOG76472W.
    PhylomeDBiQ9NXS2.
    TreeFamiTF315071.

    Family and domain databases

    InterProiIPR007484. Peptidase_M28.
    [Graphical view]
    PfamiPF04389. Peptidase_M28. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9NXS2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRSGGRGRPR LRLGERGLME PLLPPKRRLL PRVRLLPLLL ALAVGSAFYT    50
    IWSGWHRRTE ELPLGRELRV PLIGSLPEAR LRRVVGQLDP QRLWSTYLRP 100
    LLVVRTPGSP GNLQVRKFLE ATLRSLTAGW HVELDPFTAS TPLGPVDFGN 150
    VVATLDPRAA RHLTLACHYD SKLFPPGSTP FVGATDSAVP CALLLELAQA 200
    LDLELSRAKK QAAPVTLQLL FLDGEEALKE WGPKDSLYGS RHLAQLMESI 250
    PHSPGPTRIQ AIELFMLLDL LGAPNPTFYS HFPRTVRWFH RLRSIEKRLH 300
    RLNLLQSHPQ EVMYFQPGEP FGSVEDDHIP FLRRGVPVLH LISTPFPAVW 350
    HTPADTEVNL HPPTVHNLCR ILAVFLAEYL GL 382
    Length:382
    Mass (Da):42,924
    Last modified:May 18, 2010 - v2
    Checksum:i2DCA79BED029DD4B
    GO
    Isoform 2 (identifier: Q9NXS2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         118-211: Missing.

    Show »
    Length:288
    Mass (Da):32,912
    Checksum:i9F23194B62336AD3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti295 – 2951I → T in BAD96356. 1 PublicationCurated
    Sequence conflicti321 – 3211F → S in BAA90938. (PubMed:14702039)Curated
    Sequence conflicti321 – 3211F → S in BAD18747. (PubMed:14702039)Curated
    Sequence conflicti334 – 3341R → G in BAD96356. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti214 – 2141P → L.
    Corresponds to variant rs28708996 [ dbSNP | Ensembl ].
    VAR_034937

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei118 – 21194Missing in isoform 2. 1 PublicationVSP_054066Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK000091 mRNA. Translation: BAA90938.1.
    AK172764 mRNA. Translation: BAD18747.1.
    AK222636 mRNA. Translation: BAD96356.1.
    AC007191 Genomic DNA. No translation available.
    BC011553 mRNA. Translation: AAH11553.1.
    CCDSiCCDS12672.1. [Q9NXS2-1]
    CCDS54282.1. [Q9NXS2-3]
    RefSeqiNP_001156849.1. NM_001163377.1. [Q9NXS2-3]
    NP_060129.2. NM_017659.3. [Q9NXS2-1]
    UniGeneiHs.631556.

    Genome annotation databases

    EnsembliENST00000012049; ENSP00000012049; ENSG00000011478. [Q9NXS2-1]
    ENST00000366382; ENSP00000387944; ENSG00000011478. [Q9NXS2-3]
    GeneIDi54814.
    KEGGihsa:54814.
    UCSCiuc010ekn.3. human. [Q9NXS2-1]

    Polymorphism databases

    DMDMi296452875.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK000091 mRNA. Translation: BAA90938.1 .
    AK172764 mRNA. Translation: BAD18747.1 .
    AK222636 mRNA. Translation: BAD96356.1 .
    AC007191 Genomic DNA. No translation available.
    BC011553 mRNA. Translation: AAH11553.1 .
    CCDSi CCDS12672.1. [Q9NXS2-1 ]
    CCDS54282.1. [Q9NXS2-3 ]
    RefSeqi NP_001156849.1. NM_001163377.1. [Q9NXS2-3 ]
    NP_060129.2. NM_017659.3. [Q9NXS2-1 ]
    UniGenei Hs.631556.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3PB4 X-ray 1.13 X 53-382 [» ]
    3PB6 X-ray 1.05 X 53-382 [» ]
    3PB7 X-ray 1.40 X 53-382 [» ]
    3PB8 X-ray 1.13 X 53-382 [» ]
    3PB9 X-ray 1.12 X 53-382 [» ]
    ProteinModelPortali Q9NXS2.
    SMRi Q9NXS2. Positions 70-382.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120171. 14 interactions.
    IntActi Q9NXS2. 5 interactions.
    STRINGi 9606.ENSP00000012049.

    Protein family/group databases

    MEROPSi M28.016.

    Polymorphism databases

    DMDMi 296452875.

    Proteomic databases

    MaxQBi Q9NXS2.
    PaxDbi Q9NXS2.
    PRIDEi Q9NXS2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000012049 ; ENSP00000012049 ; ENSG00000011478 . [Q9NXS2-1 ]
    ENST00000366382 ; ENSP00000387944 ; ENSG00000011478 . [Q9NXS2-3 ]
    GeneIDi 54814.
    KEGGi hsa:54814.
    UCSCi uc010ekn.3. human. [Q9NXS2-1 ]

    Organism-specific databases

    CTDi 54814.
    GeneCardsi GC19P046195.
    H-InvDB HIX0015239.
    HGNCi HGNC:25952. QPCTL.
    HPAi HPA040797.
    neXtProti NX_Q9NXS2.
    PharmGKBi PA134922163.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG298226.
    HOGENOMi HOG000189291.
    HOVERGENi HBG009812.
    InParanoidi Q9NXS2.
    OMAi QTYFRKD.
    OrthoDBi EOG76472W.
    PhylomeDBi Q9NXS2.
    TreeFami TF315071.

    Miscellaneous databases

    EvolutionaryTracei Q9NXS2.
    GenomeRNAii 54814.
    NextBioi 57549.
    PROi Q9NXS2.

    Gene expression databases

    ArrayExpressi Q9NXS2.
    Bgeei Q9NXS2.
    CleanExi HS_QPCTL.
    Genevestigatori Q9NXS2.

    Family and domain databases

    InterProi IPR007484. Peptidase_M28.
    [Graphical view ]
    Pfami PF04389. Peptidase_M28. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Colon.
    2. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Cerebellum.
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Kidney.
    5. "Isolation of an isoenzyme of human glutaminyl cyclase: retention in the Golgi complex suggests involvement in the protein maturation machinery."
      Cynis H., Rahfeld J.U., Stephan A., Kehlen A., Koch B., Wermann M., Demuth H.U., Schilling S.
      J. Mol. Biol. 379:966-980(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Structures of human Golgi-resident glutaminyl cyclase and its complexes with inhibitors reveal a large loop movement upon inhibitor binding."
      Huang K.F., Liaw S.S., Huang W.L., Chia C.Y., Lo Y.C., Chen Y.L., Wang A.H.
      J. Biol. Chem. 286:12439-12449(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) OF 53-382 IN COMPLEXES WITH SYNTHETIC INHIBITORS PBD150 AND ZINC IONS, FUNCTION, CATALYTIC ACTIVITY, COFACTOR.

    Entry informationi

    Entry nameiQPCTL_HUMAN
    AccessioniPrimary (citable) accession number: Q9NXS2
    Secondary accession number(s): Q53HE4, Q96F74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3